DNA Denaturation by:

-Heat

-Chemicals (e.g. formamide)

-High pH

Melting Temperature is dependent on:

-Length of DNA (for short fragments)

-Base composition

Topic 2, Nucleic acids, genes, and genomes

Kinetics of Renaturation

*

*

highly repetitive DNA –very low Cot

repetitive DNA –low Cot

non-repetitive DNA –higher Cot

0 2 4

Yeast artificial chromosome

Bacterial artificial chromosome

Sequence tagged sites

Mapping with STSs

Figure 24.11

“Shotgun sequencing and BAC walking”

Physical distance versus genetic map distance

What explains the conservation of gene order over large evolutionary distance?

Figure 1: Neurospora has a low proportion of genes in multigene families.

Figure 2: Neurospora possesses few highly similar genes.

Figure 3: Example of lack of recent duplications in a Neurospora gene family (MFS sugar transporters).

Figure 4: Correspondence between predicted RIP, methylation and repetitive DNA.

Figure 5: Overview of major intracellular signaling pathways in Neurospora.