RESEARCH POSTER PRESENTATION DESIGN © 2011

www.PosterPresentations.com

QUICK DESIGN GUIDE (--THIS SECTION DOES NOT PRINT--)

This PowerPoint 2007 template produces a 36”x56”

professional poster. It will save you valuable time

placing titles, subtitles, text, and graphics.

Use it to create your presentation. Then send it to

PosterPresentations.com for premium quality, same

day affordable printing.

We provide a series of online tutorials that will guide

you through the poster design process and answer

your poster production questions.

View our online tutorials at:

http://bit.ly/Poster_creation_help

(copy and paste the link into your web browser).

For assistance and to order your printed poster call

PosterPresentations.com at 1.866.649.3004

Object Placeholders

Use the placeholders provided below to add new

elements to your poster: Drag a placeholder onto the

poster area, size it, and click it to edit.

Section Header placeholder

Use section headers to separate topics or concepts

within your presentation.

Text placeholder

Move this preformatted text placeholder to the poster

to add a new body of text.

Picture placeholder

Move this graphic placeholder onto your poster, size it

first, and then click it to add a picture to the poster.

QUICK TIPS (--THIS SECTION DOES NOT PRINT--)

This PowerPoint template requires basic PowerPoint (version 2007 or newer) skills. Below is a list of commonly asked questions specific to this template. If you are using an older version of PowerPoint some template features may not work properly.

Using the template Verifying the quality of your graphics Go to the VIEW menu and click on ZOOM to set your preferred magnification. This template is at 100% the size of the final poster. All text and graphics will be printed at 100% their size. To see what your poster will look like when printed, set the zoom to 100% and evaluate the quality of all your graphics before you submit your poster for printing. Using the placeholders To add text to this template click inside a placeholder and type in or paste your text. To move a placeholder, click on it once (to select it), place your cursor on its frame and your cursor will change to this symbol: Then, click once and drag it to its new location where you can resize it as needed. Additional placeholders can be found on the left side of this template. Modifying the layout This template has four different column layouts. Right-click your mouse on the background and click on “Layout” to see the layout options. The columns in the provided layouts are fixed and cannot be moved but advanced users can modify any layout by going to VIEW and then SLIDE MASTER. Importing text and graphics from external sources TEXT: Paste or type your text into a pre-existing placeholder or drag in a new placeholder from the left side of the template. Move it anywhere as needed. PHOTOS: Drag in a picture placeholder, size it first, click in it and insert a photo from the menu. TABLES: You can copy and paste a table from an external document onto this poster template. To make the text fit better in the cells of an imported table, right-click on the table, click FORMAT SHAPE then click on TEXT BOX and change the INTERNAL MARGIN values to 0.25

Modifying the color scheme

To change the color scheme of this template go to the

“Design” menu and click on “Colors”. You can choose

from the provide color combinations or you can

create your own.

© 2011 PosterPresentations.com 2117 Fourth Street , Unit C Berkeley CA 94710 posterpresenter@gmail.com

Student discounts are available on our Facebook page.

Go to PosterPresentations.com and click on the FB icon.

DNA EXTRACTION FROM SOIL IN THE FRAME WORK OF GENDER-SPECIFIC ENVIRONMENTAL EXPOSURE TO

Coccidioides spp.

Coccidioidomycosis, also known as valley fever, is an

infection caused by inhalation of Coccidioides spp. spores.

Coccidioides spp. are dimorphic, soil-dwelling fungi, known

to cause a broad spectrum of disease ranging from a mild

febrile illness to severe pulmonary manifestations or

disseminated disease.

The genus Coccidioides is comprised of two genetically

distinct species: Coccidioides immitis and C. posadasii.

These two species cause similar clinical symptoms;

however, they are present in different geographic regions.

C. immitis is found in central and southern California, with

the San Joaquin Valley being the region of greatest

endemicity.

The most concentrated region for C. posadasii is in Arizona

and sporadic sites in southern Utah and Nevada, Oregon,

and El Paso, TX. Beyond the USA, C. posadasii is present in

parts of Mexico and Central and South America. Members of

our group has been working for the last year in identifying

the epidemiologic and clinical/radiographical presentations

of Coccidiomycosis in the Permian Basin.

This is of great concern to women of childbearing age that

become infected prior to conception, as the risk of

dissemination increases during pregnancy and requires

increased vigilance.1

Introduction

Irene Schwaninger1, Stacy Martinez1, Maira Carrillo2, Natalia Schlabritz-Loutsevitch2 and Javier Flores-Guardado 3

1 Office of Medical Education, TTUHSC at the PB 2 Department of Obstetrics and Gynecology and 3 Department of Internal Medicine TTUHSC at the Permian Basin

The goal of this study was to evaluate the soil

microbiome, including those of Coccidiodes spp. in soil

from the counties served by Texas Tech University Health

Sciences Center at the Permian Basin.

OBJECTIVES

Six soil samples were collected at Loop 250 and Lamesa

Rd, Midland, TX Midland County. Using an N-99 respirator

and sterile techniques, approximately 10 grams of soil at

a depth of 10 inches was collected from an established

Prairie Dog colony. DNA was then isolated using Mo-Bio

PowerLyzer Power Soil kit (Carlsbad, CA, U.S.A.).

Methods and Materials

RESULTS

CONCLUSIONS

The DNA extraction from the soil presents a more

challenging methodology compared to human tissue

DNA extraction.

The soil pH and matrix should be taken in

consideration during extraction process, which may

require additional processing.

While the DNA controls worked for the Q-RT PCR, all

soil samples were negative for the gene used as a

known control. REFERENCES

1 Arch Pathol Lab Med. 2007; 131,:652-655. Disseminated Coccidioidomycosis in Pregnancy.

Hooper, JE, Lu, Q, Pepkowitz, S.

2 Litvintseva et al. 2015. Investigating the expanding geographic range of coccidioides

[PowerPoint Slides].

ACKNOWLEDGEMENT

This study was supported by the Regional Dean funds to Dr. Guardado.

Additional support and funding was provided by the Department of Obstetrics and Gynecology

TTUHSC, Permian Basin.

From: Litvintseva et al, CID 2014 2

From: Litvintseva et al, CID 2014 2

Real- time Quantitation PCR was performed on the

isolated DNA samples. The samples were quantified

using a Standard Curve and detected utilizing FastStart

Essential DNA Green Master (Roche, Indianapolis,

Indiana, U.S.A.).

Figure 2a) This is the amplification curve for sample 1. The negative sample is a bit noisy but does not pass the minimal EPF to be considered positive. The amplification is late in the cycle indicating how little of coccidiosis’s species gene is in the sample.

Figure 2b) This is the melting peak for sample 1. The expected melting temperature the product is about 85˚C. The sample has a melting temperature of about 85˚C showing this is our expected product.

Figure 1 shows the Standard Curve 10- Fold Change of the Coccidioides immitus control (Vircell, Granada, Spain) . The dilutions were 1:1 (60,000 copies/ rxn), 1:10 (6,000 copies/ rxn), 1:100 (600 copies/ rxn), and 1:1000 (6 copies / rxn).

A B