dna isolation. onion dna isolation dna thought for the day: “the capacity to blunder slightly is...
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Onion DNA Isolation
DNA Thought for the day:“The capacity to blunder slightly is the real marvel of DNA. Without this special attribute, we would still be anaerobic bacteria and there would be no music.” ~Lewis Thomas
Human cheek Cells
Why should the DNA extraction procedure be universal and able to be used on all
types of cells?
What Electrical Charge Does DNA Have?
When we visualize DNA we need thousands of molecules of DNA to come together.
Why would charge be important?
The knobs do not correspond to any temperature!
600c is critical!
Chopped Onion
Note consistency: The liquid should become thicker
DNA Isolation Procedure
Step 1: Mash piece of onion well for 2 minutes. Place in Ziploc bag
Step 2: Pour ~20mL of detergent solution into the bag.
Step 3: Add two shakes of meat tenderizer to the bag. Mash together for 1 minute.
Step 4: Place bag in hot water bath at 55-60O C for 15 min.
Caution – Do not let temp of water bath exceed 60O C and no less than 50O C.
Step 5: Place bag in ice water bath for 5-10 min to get the Temperature of the mixture down to 10O C.
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