Download - 2013 HID University Introduction
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Introduction: Increasing Success Rates & Efficiency from Crime Scene to Courtroom Ariana Wheaton Senior Market Development Manager - Americas Life Technologies
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Increased Efficiency
Ease of Use Success with Difficult Samples
Simplified Interpreta;on
Higher Throughput
Streamlined Workflow
Technology Improvements Over Last 7 Years…
Increased Integra9on and Efficiency
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Time to Result
(hrs/per 24 samples) ~1-‐3.5 hrs ~0-‐2 hrs ~4 hrs ~1.5 hrs ~1 hrs
Improved Efficiency for Single Source Sample Processing
Time to Result
(per 24 samples) 0 0 ~40 mins ~35 mins ~5-‐10 mins
Total Time to Result = ~7-‐12 hrs
Collec;on Extrac;on Quan;ta;on/ Normaliza;on Amplifica;on Detec;on Analysis
Total Time to Result = ~1.5 hrs
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Total Time to Result = ~10-‐58 hrs
Sample Prepara;on
Quan;ta;on/ Normaliza;on Amplifica;on Detec;on Analysis
Time to Result
(hrs/per 6-‐16 samples) ~1-‐48 hrs ~2 hrs ~3.5 hrs ~0.75 hrs ~2-‐3 hrs Time to Result
(hrs/per 13 samples) ~1-‐3 hrs ~1.5 hrs ~1.5 hrs ~0.55 hrs ~1-‐2 hrs
Total Time to Result = ~6-‐9 hrs
Dependent on sample type and method
Improved Efficiency for Casework Sample Processing
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A lot of progress… But how much have we increased your casework success rates?
Increased Efficiency
Ease of Use Success with Difficult Samples
Simplified Interpreta;on
Higher Throughput
Streamlined Workflow
Technology Improvements Over Last 7 Years…
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Results from recent Forensic Magazine survey… Failure rate following Amplification/CE
Overall average from North American Crime Labs (n=126)
§ 20% - Note: Labs processing higher % of property crimes and touch DNA have much higher failure rates.
Ø We did a similar survey in 2006, and the average failure rate was also 20%!
Ø Survey indicated failure rates have not been reduced, but this is due to increase in property crime processing and more challenging samples (i.e. touch samples)
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What is the cost of failed samples?
§ 2006 study* in the USA estimates processing a single piece of evidence through a DNA workflow takes on average: § 157.6 minutes of analyst and labor time § Anywhere between $425 and $1,720
§ In addition, outsourcing labs typically charge the same flat fee for service whether or not the submitted sample yields probative or useful information
*Source: Cost Study of DNA Testing and Analysis North Carolina DOJ OSBM March 2006
Increasing success rates in the lab means more efficient use of investigative and laboratory resources
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What contributes to the total processing cost for a DNA evidence sample?
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
Typical Cost Breakout*
Labor Cost/Time Facility Overhead Shipping Reagents/Supplies
Most impacted by 1st Pass Success Rate
*Actual cost breakout will vary by laboratory
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The “Hidden Cost” of Low First Pass Success Rates
Higher 1st Pass Success Rate = Less Re-‐runs
Lower 1st Pass Success Rate = More Re-‐runs
Reduced Labor & Consumable Cost
Increased Labor & Consumable Cost
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Detection & Analysis
Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info.
LIFE Offers Complete Integrated HID Workflow
GeneMapper® ID-X Expert System
Software
Next Generation STR Kits
3500/3500xL Genetic Analyzer
3130/3130xl Genetic Analyzer
Extraction Quantification Amplification
7500 Real-Time PCR Instrument
Next Generation Quantifiler® Kits
PrepFiler™ Forensic DNA Extraction kit
Veriti® 96-Well Thermal Cycler
GeneAmp® PCR System 9700 Cycler
AutoMate Express™
Collection
Copan 4N6FLOQSwab™
& NUCLEIC-CARD™
Screening
ParaDNA®
Converge™ Software DNA Workflow
Kinship and Paternity
Direct Search Familial Search
NEW
NEW
NEW
NEW
Converge™
Software
NEW
NEW
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Strategies for Increased Downstream Success Rates:
Improve Collection & Sample Selection Collection
4N6FLOQSwabs™ Patented technology to maximize DNA
collection and elution efficiency; enables significantly improved profile success
rates compared to cotton swabs
Screening Sample to Answer Casework Screening Device to help determine which samples
contain the highest likelihood of generating a probative DNA profile in a
laboratory workflow
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NUCLEIC-CARD™ System—Now Available Globally
§ Scheduled to A reliable device for collection, preservation, and long-term storage of nucleic acids
Chemically-treated matrix to enable cell lysis and protein denaturation
NUCLEIC –CARD™ NUCLEIC –CARD™ COLOR
NUCLEIC –CARD™ Collection Device
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Quantification – Areas for Improvement
Predic9on of M:F Mixture Ra9o
Improve Sensi9vity and Reproducibility
Predict Sample Quality
Match Performance to Newer Genera9on STR kits
Improve DNA Standard Consistency
Faster Time to Result
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Quantifiler® Kits Product Family
Quantifiler® Duo Kit Quantifiler® Human Kit
Quantifiler® Y Human Kit
Three kits – for different applications • Quantifies DNA from wide variety of sources • Works with wide variety of DNA input • Sample Extraction chemistry compatible • Guides selection of optimal AmpFSTR®
PCR Amplification Kit • Accurate, Sensitive & Specific
Next Gen Quantification
Two new Quantifiler® Kits in 2013 +
Flexible workflow software
To integrate with Next Generation STR kits
Quantifiler® HP Kit Quantifiler® Trio Kit
HID RT v1.2 Software
NEW
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The GlobalFiler™ Kit: Going Further for Casework
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Additional 6-dye Application: Yfiler® Plus Kit
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Fall 2013
Yfiler® Plus: The First 6-Dye, 27 Marker Y-STR Multiplex
Plus in Power of Discrimination
• All 17 loci amplified by the Yfiler® kit • 10 new Y-STR loci including 7 Rapidly Mutating1 Y-STRs for ability to
discriminate between close male relatives • 11 mini STRs below 220bp
Plus in Performance
• Improved sensitivity & results with challenging casework samples • Improved recovery of alleles and intracolor balance in male/female
mixtures containing high female background • Improved resolution capacity for male/male mixtures • Expanded allelic ladder • Shorter amplification times • Direct amplification protocol with single-source samples
1. Rapidly Mutating Y-STRs have mutation rates above 1 x 10-2 per marker per generation. 17 Yfiler Y-STRs show mutation rates between 1 x 10-3 and 1 x 10-4 per marker per generation. The content provided herein may relate to products that have not been officially released and is subject to change without notice.
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Ion Torrent™ Semiconductor Sequencing for Forensic Applications
Casework Single Source
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Mitochondrial DNA Sequencing
SNP Genotyping Paternity, Mixtures, Missing
Persons, DVI
Ancestral & Phenotypic SNPs for Inves;ga;ve Leads
Eye Color
Hair Color
Missing Persons, DVI Facial Reconstruc;on
Looking ahead… Poten9al PGM™ Human Iden9fica9on Applica9ons
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Webinar hosted by Forensic Magazine Ion Torrent™ Semiconductor Sequencing for Forensic Applica9ons
Lisa Calandro Director of Product Management Life Technologies
Dr. Walther Parson Institute of Legal Medicine University of Innsbruck, Austria
Available to view “on-demand”: www1.gotomeeting.com/register/134635184
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HID Workflow Software Solutions
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Converge™ Software - Integrate & Manage All Your Data, Analysis & Reporting Tools
§ Modular system means easier deployment of new: − Analysis tools (i.e. Paternity/Kinship, Database & Familial Searching) − Lab management tools − Data management tools − Reporting tools
Long term goal of Converge™ Centralize and integrate data, analysis and reporting across all points of the forensic DNA workflow including:
− At the crime scene − At the police agency − At the crime lab − In the court room
Converge™
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Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info.
More Workflow Efficiency
Higher Success
Rates
More
Information
4N6FLOQSwab™ &
NUCLEIC-CARD™ Collection Devices
ü
ParaDNA® Screening System ü
Next Gen Quantifiler® Kits ü ü ü
GlobalFiler® Kits ü ü ü
Yfiler® Plus Kit ü ü ü
Converge™ Software ü ü
PGM™ for Forensics ü
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Today’s Agenda
Most of the presentations will be posted to SlideShare: http://www.slideshare.net/Lifetech_HID
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THANK YOU!!
Ariana Wheaton Senior Market Development Manager-Americas Life Technologies Phone: 650.638.6317 Email: [email protected]
FOR FORENSIC OR PATERNITY USE ONLY. ©2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. The content provided herein may relate to products that have not been officially released and is subject to change without notice.