MFSH MFSH 20032003
PRESS F1F1 FOR GUIDEANCE
Enterobacteria, Vibrio, Campylobacter & Enterobacteria, Vibrio, Campylobacter & Pseudomonas Pseudomonas
EnterobacteriaEnterobacteria 33Station 1: Gram stain of Enterobacteria 4Station 2: NLF + LF on Mc Conkey 5Station 3: Klebsiella on Mc Conkey 6Station 4: E.coli on Mc Conkey 7Station 5: Pure LF & Pure NLF on both Mc Conkey & DCA 8Station 6: Proteus on Blood agar 9Station 7: Selenite F broth 10Station 8: Indole production 11Station 9: H2S Production 12Station 10: Widal test 13Station 11: Treatment & diseases 14VibrioVibrio 1515Station 11: Gram stain of Vibrio cholera 16Station 12: Growth on TCBS 17Station 13: Growth on TCBS & alkaline peptone water 18CampylobacterCampylobacter 1919Station 14: Gram stain of Campylobacter 20Station 15: Urease Production 21PseudomonasPseudomonas 2222Station 16: Growth on Nutrient agar 23Station 17: Sensitivity test 24Station 18: Growth on blood media 25Station 20: Treatment and diseases 26
E.g.E.g.
E.coliE.coli
KlebsiellaKlebsiella
ProteusProteus
ShigellaShigella
SalmonellaSalmonella
and others.and others.
Non lactose Non lactose fermentersfermenters
Station 1Station 1
NLF= non lactose fermenters
LF= lactose fermenters
NLF
LF
Station 2Station 2
Large mucoid colonies Large mucoid colonies (pinkish / reddish)(pinkish / reddish)
Station 3Station 3
Pink coloniesPink colonies
Station 4Station 4
PinkishPinkish
PinkishPinkish
PalePale
PalePale
LF (e.g. E-coli & Klepsilla)LF (e.g. E-coli & Klepsilla)
N.B. it is E-coli here N.B. it is E-coli here
““Shape of colonies”Shape of colonies”
LFLF
NLF (e.g. Salmonella, Shigella)NLF (e.g. Salmonella, Shigella)
NLFNLF
Station 5Station 5
Station 6Station 6
Samonella Samonella
andand
ShigellaShigella
Station 7Station 7
Station 8Station 8
Blacke.g. shigella
Station 9Station 9
1/10 1/20 1/40 ……… 1/640
+ test+ test- test- test
1/10 1/20 1/401/40
++ --
1/10 1/20 1/40 1/80 1/160 1/3201/320++ --
There is more than 4 folds increase in the titer of antibodies against Salmonella typhi “320/40= 8”, this means acute infection.
Station 10Station 10
Escherichis coli
Infection:-
1- urinary tract infection
2- diarrhea
Treatment:-
requires antibiotic testing, but most common are
1-”Ampicilin”
2-”Aminglycosides”
Klebsiella
Infection:-
urinary tract infection
Treatment:-
beta lactamase producing
“Aminglycosides” is used
Proteus
Infection:-
urinary tract infection, by the urease the urea is split to produce ammonia “alkaline urine”
Salmonella
Infection:-
1- typhoid fever
2- food poising “meat, poultry & egg” diarrhea
This usually happens when people eat in public places “Pakistan, Sudan & Egypt”
Treatment:-
In enteric fever “chloramphenicol”, if resistant “ciprofloxacilin”
Shigilla
Infection:-
dysenteriae (enterotoxin, cytotoxic & neurotoxic) bloody, mucoid & small stools with fever
Treatment:-
Fluid replacement & “Ampicillin”
Station 11Station 11
E.g.E.g.
Vibrio CholeraeVibrio Cholerae
Station 12Station 12
Station 13Station 13
aureus
Mentioned Mentioned previouslypreviously
Clear Clear
TurbidTurbid
Station 14Station 14
)vibrio(
E.g.E.g.
Campylobacter jejuni Campylobacter jejuni
Station 15Station 15
Helicobacter pyloriHelicobacter pylori
Station 16Station 16
No pigment
Station 17Station 17
SensitiveSensitive
SensitiveSensitive
Pseudomonas is resistant to Pseudomonas is resistant to many antibiotics “but many antibiotics “but sensitive to amino glycosides sensitive to amino glycosides (e.g. gentamicin) and some (e.g. gentamicin) and some others” others”
Station 18Station 18
Note that Pseudomonas is strictly aerobic
Green orange
Station 19Station 19
Vibrio cholera
Infection:-
cholera (exotoxin persistent and excessive secretion of isotonic fluid by intestinal mucosa)
Treatment:-
“Erythromycin”
Campylobacter jejuni
Infection:-
Food poisoning “Chicken” diarrhea.
Treatment:-
“Erythromycin”
Helicobacter pylori
Infection:-
Gastric and duodenal ulcerations
Treatment:-
Triple therapy: “Omeprazole” + “Amoxycilin” + “Metronidazole”
Quadruple therapy: Same + proton pump inhibitors
Pseudomonas
Infection:-
Gastric and duodenal ulcerations
Treatment:-
“Erythromycin”, “Amoxycilin”
Station 20Station 20
يا يا دعواتكم دعواتكم
شبابشباب
A word can change the outcome of A word can change the outcome of
many things. So, if you either liked or many things. So, if you either liked or
didn’t like this project, why keep your didn’t like this project, why keep your
thoughts to yourself ?thoughts to yourself ?
Send us your comments and ideas Send us your comments and ideas
to to [email protected], It will , It will
mean too much to us, mean too much to us, “even if it was “even if it was
just one word”just one word”
THANXTHANX
M. F. ShaheenM. F. Shaheen 2003 2003