Background References

1. Ericksen B. et al. Antibacterial activity

and specificity of the six human

alpha-defensins. Antimicrob. Agents

Chemother. 49:269-275(2005).

2. Zou G. et al. Toward understanding the

cationicity of defensins. Arg and Lys

versus their noncoded analogs. J. Biol.

Chem. 282:19653-19665(2007).

Product Type: Mouse monoclonal IgG1, primary antibodies

Species reactivity: Human, Rat

Applications： WB, IHC

Molecular Wt.： 10 kDa

Clone number: 2D2

Description： Defensins are a family of antimicrobial and cytotoxic

peptides thought to be involved in host defense. They are abundant

in the granules of neutrophils and also found in the epithelia of

mucosal surfaces such as those of the intestine, respiratory tract,

urinary tract, and vagina. Members of the defensin family are highly

similar in protein sequence and distinguished by a conserved

cysteine motif. The protein encoded by this gene, defensin, alpha

1, is found in the microbicidal granules of neutrophils and likely

plays a role in phagocyte-mediated host defense. Several alpha

defensin genes are clustered on chromosome 8. This gene differs from

defensin, alpha 3 by only one amino acid. This gene and the gene

encoding defensin, alpha 3 are both subject to copy number

variation.

Immunogen：

Peptide.

Positive control：

Rat spleen tissue, human spleen tissue.

Subcellular location：

Secreted.

Database links：

SwissProt: P59665(Human)

Recommended Dilutions：

WB: 1:500-1:2,000 IHC: 1:50-1:200

Storage Buffer：

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium

Azide.

Storage Instruction：

Store at +4°C after thawing. Aliquot store at -20°C or -80°C.

Avoid repeated freeze/thaw cycles.

Purity：

ProG affinity purified.

HNP-1

Fig1: Western blot analysis of HNP-1 on rat

spleen tissue lysates using anti-HNP-1

antibody at 1/500 dilution.

Fig2: Immunohistochemical analysis of

paraffin-embedded human spleen tissue using

anti-HNP-1 antibody. Counter stained with

hematoxylin.

rev. 01/03/18

Cat#: EM1701-52