Download - Lab equipment
![Page 1: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/1.jpg)
b
Laboratory equipment
Prepared bySamira fattah
Assis. Lec.College of health sciences-HMU
Lecture 2
![Page 2: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/2.jpg)
What Is a Laboratory?
• A laboratory is a room or a place equipped for the performance of tests, experimentation, investigative processes .
![Page 3: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/3.jpg)
Medical laboratory
• A medical laboratory or clinical laboratory is a laboratory where tests are done on clinical specimens in order to get information about the health of a patient as pertaining to the diagnosis, treatment, and prevention of disease.
![Page 4: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/4.jpg)
Lab equipment
Lab. equipment refers to the various tools used by scientists working in a laboratory.
![Page 5: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/5.jpg)
MicroscopyWhat is a microscope?• Is an optical instrument consisting of a combination
of lenses which magnifies the image of the object seen through it.
• It is used for the morphological study of a very small organisms which are not visible by naked eye.
Micro= small scope=to view
![Page 6: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/6.jpg)
Types of microscopes
• Simple• Compound• Electron
![Page 7: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/7.jpg)
Incubator
• Is a device used to grow and maintain microbiological cultures.
• The incubator maintains optimal temperature,
humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.
![Page 8: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/8.jpg)
Incubator
![Page 9: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/9.jpg)
Autoclave
• An autoclave is a pressure chamber used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the load and the contents.
• Used to sterilize culture media, discard, and other equipments.
![Page 10: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/10.jpg)
Autoclave
![Page 11: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/11.jpg)
Oven
• device used in sterilization.• oven uses dry heat to sterilize.• It used to sterilize items that might be damaged by
moist heat (e.g., glasswares, powders, oils).
![Page 12: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/12.jpg)
![Page 13: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/13.jpg)
Laboratory refrigerator
Is used for a wide variety of purposes such as:
• maintenance and storage of stock culture between subculturing periods.
• storage of sterile media to prevent dehydration.
• also used as repository for thermolable solutions, antibiotics and serums.
![Page 14: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/14.jpg)
Lab. refrigerator
![Page 15: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/15.jpg)
Centrifuge
• is an apparatus that rotates at high speed and separates substances of different densities.
![Page 16: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/16.jpg)
![Page 17: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/17.jpg)
Balance
• used to measure an object’s mass to a very high degree of precision.
![Page 18: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/18.jpg)
hot plate / stir plate
• used to heat and stir substances.
Magnetic stirring bars
![Page 19: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/19.jpg)
Water bath
• is a device that maintains water at a constant temperature.
• It is used in the microbiological laboratory for incubations.
![Page 20: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/20.jpg)
Biological Safety Cabinets
• is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with pathogens.
![Page 21: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/21.jpg)
![Page 22: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/22.jpg)
Bunsen burner
is a common piece of laboratory equipment that produces a single open gas flame, which is used for heating and sterilization.
![Page 23: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/23.jpg)
Inoculating loops and needles• Inoculating loops are used to transfer microorganisms to
growth media or for staining slides.
• The wire forms a small loop with a diameter of about 5 mm.• The loop of wire at the tip may be made of platinum or
nichrome.
![Page 24: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/24.jpg)
• Needles are straight wires (no loop) used to pick up bacteria from closely packed colonies or to inoculate in a very defined area.
• needles commonly used to inoculate semi-soft media.
![Page 25: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/25.jpg)
![Page 26: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/26.jpg)
Anaerobic jar
• is an instrument used in the production of an anaerobic environment.
• This method of anaerobiosis is used to culture bacteria which die or fail to grow in presence of oxygen.
![Page 27: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/27.jpg)
![Page 28: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/28.jpg)
Glassware Glass slide and cover slip
• Glass slide: used to place specimens on to observe under the microscope.
• Cover slip: used to cover specimens on a microscope slide.
![Page 29: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/29.jpg)
slide and coverslip
![Page 30: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/30.jpg)
Glassware Petri dishes
• often used to make agar plates for microbiology studies.• The dish is partially filled with warm liquid containing agar and a mixture of
specific ingredients that may include:• nutrients• blood• salts• Carbohydrates• dyes• indicators• amino acids or• antibiotics
![Page 31: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/31.jpg)
Glassware
• Pipet and Graduated Cylinders
• Glass or plastic• Used to measure liquid volume.• Graduated in ml.
![Page 32: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/32.jpg)
Graduated Cylinders Pipet
![Page 33: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/33.jpg)
Glassware
Beaker • Glass or plastic• Used to stir, heat(if glass) and measure liquid volume
in ml.
![Page 34: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/34.jpg)
Glassware
Flask • Glassware used to heat and store substances.
![Page 35: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/35.jpg)
Glassware
Funnel aids in pouring liquids into small openings without
spilling them.
![Page 36: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/36.jpg)
Glassware • Test tube used to mix, heat or store substances.
• Test tube rack used to hold test tubes.
![Page 37: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/37.jpg)
wash Bottles
• used to rinse various pieces of laboratory glassware.
![Page 38: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/38.jpg)
Filter paper
• special paper used to separate solids from liquids.
![Page 39: Lab equipment](https://reader034.vdocument.in/reader034/viewer/2022042907/5883b2521a28ab3b488b739d/html5/thumbnails/39.jpg)
End