Download - Lecture 37 Vesicle Transport in Neurons
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8/17/2019 Lecture 37 Vesicle Transport in Neurons
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B. Katz
Lecture 37:
vesicle transport
and the
neuromuscular
junction
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Announcements! And other important info!
• Last lecture quiz today
• Lab presentations today
•
Lab notebooks, hard copy andvirtual, due today
• Creative Biology assignment 5/
• evie" session poll "ill be posted
• #inal $%am &onday 5/'', ( am
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Lodish et) al, Molecular Cell Biology)
*sing mutant yeast to identi+y proteins involved in the
secretory path"ay
For each class of mutant:
A. Determine which part of the pathway is defective
B. Identify multiple proteins that could be defective in each case
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Non-peptide
(e.g. Ach)
Peptide (e.g.
endorphins)
n a motor neuron, "hat types o+ cargo need to be
transported to the a%on terminus-
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Coupled transport
Molecular Biology of the Cell (© Garland Science 2008)
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.econdary active transport
Molecular Biology of the Cell (© Garland Science 2008)
∆G’ > 0 for transport of X
∆G’ < 0 for another reaction
∆G’ < 0
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e%tracellular space
cytosol
.econdary active transport 0a1 / glucose symporter
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Na+
Na+Choline/Na+ symporter
Post-synaptic
muscle cell
Acetylcholinereceptor
Vesicular acetylcholine/H+ antiporter
Acetylcholine
H+H+H+
Vesicle containingacetylcholine
H+
ATP
ADP
Axon terminus
Pre-synapticmotor neuron
Glucosetransporter
H+
H+
H+
ATPasepump H
+
H+
ADP ATP
H+
Vesicle
Microtuule-
+
-
+
Voltage-gate!Ca"+ channel
Ca"+
Ca"+
Ca"+
Ca"+
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Copyright © 2011 Wolters Kluwer Health ippincott Willia!s " Wil#ins
&odel 23 acetylcholine packaging and release
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Na+
Na+Choline/Na+ symporter
Post-synapticmuscle cell
Acetylcholinereceptor
Vesicular acetylcholine/H+ antiporter
Acetylcholine
H+H+H+
Vesicle containingacetylcholine
H+
ATP
ADP
Axon terminus
Pre-synapticmotor neuron
Glucose
transporter
H+
H+H+ ATPasepump
H+H+
ADP ATP
H+
Vesicle
Microtuule-
+
-
+
Voltage-gate!Ca"+ channel
Ca"+
Ca"+
Ca"+
Ca"+
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Copyright © 2011 Wolters Kluwer Health ippincott Willia!s " Wil#ins
4hich o+ the +ollo"ing "ould result +rom inhibition o+ the
0a1/ 1 A67ase-
A. Acetylcholine could not
be synthesized
B. H+ would not move
through the
acetylcholine
antiporterC. Acetylcholine would
not be transported into
the synaptic vesicle
D. Choline could not betransported back into
the axon terminus
E. More than one of the
above is correct
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egulation o+ vesicle docking and +usion .0A$s
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&odel 2' Ca81, .0A$s, and vesicle +usion
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Botulism to%in is a protease that cleaves synta%in and
.0A7) 9o" might this a++ect synaptic vesicles-
A. Vesicles could fuse with plasma membranes in the absence of Ca2+
B. Vesicles would be unable to stably interact with the plasma membrane
C. Ca2+ would be unable to bind synaptotagmin
D. T-SNAREs would be unable to interact with v-SNAREs
E. More than one of the above is correct
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Na+
Na+Choline/Na+ symporter
Post-synapticmuscle cell
Acetylcholinereceptor
Vesicular acetylcholine/H+ antiporter
Acetylcholine
H+H+
H+
Vesicle containingacetylcholine
H+
ATPADP
Axon terminus
Pre-synapticmotor neuron
Glucosetransporter
H+
H+H+ ATPase
pumpH+
H+
ADPATP
H+
Vesicle
Microtuule-
+
-
+
Voltage-gate!Ca"+ channel
Ca"+
Ca"+
Ca"+
Ca"+
6here is an inactivating mutation in the 91/acetylcholine
antiporter in the a%on terminal o+ a motor neuron) 4hich
o+ the +ollo"ing is true-
A) 6here "ill be a lack o+ 91 in the
secretory vesicles)
B) Acetylcholine cannot be
transported inside a vesicle)
C) 6here "ill be less release o+acetylcholine +rom the motor
neuron in response to an
upstream action potential)
:) 6here "ill be decreased +usion o+
vesicles "ith the plasma
membrane o+ the motor neuron in
response to calcium in+lu% into
the cytosol)
$) &ore than one o+ these is correct)
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:r) #rankenstein +ound a "ay to reanimate a monster
assembled +rom dead body parts he +ound "hile sneaking
around in graveyards) 9e believes that i+ he adds a mutant
synaptotagmin that is al"ays activated in the presence orabsence o+ Ca81 ions, the monster "ill be able to move)
4hy is he "rong, +ocusing entirely on the interactions
bet"een the motor neuron and the muscle cell-
Na+
Na+Choline/Na+ symporter
Post-synapticmuscle cell
Acetylcholinereceptor
Vesicular acetylcholine/H+ antiporter
Acetylcholine
H+H+H+
Vesicle containingacetylcholine
H+
ATP
ADP
Axon terminus
Pre-synapticmotor neuron
Glucosetransporter
H+
H+H+ ATPasepump
H+
H+
ADP ATP
H+
Vesicle
Microtuule
-
+
-
+
Voltage-gate!Ca"+ channel
Ca"+
Ca"+
Ca"+
Ca"+
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