Regulation of Ca++

Homeostasis in the Hearts of Female Rats

Sarah-Neïel Barama

October 19th 2006

Supervisor: Dr. Chalifour (LDI)

Background & Hypothesis Relationship b/w ♥ & hormones Ca++ Homeostasis proteins (NCX1, SERCA2a, PLB, CSQ,

CRT…) Contract = Ca++ → cytosol Relax = Ca++ → SR & ECM

Acute Stress: exercise

Oxidative stress : Doxorubicin ± Dexrazoxane

Hyp: Expression of Ca++ regulating proteins is

. Hormone-dep and is correlated to ♥ fct

Method Intact VS Ovariectomy

Sedentary or Exercised

PBS or DOX or DOX:DEX

♥ Extraction & Homogenisation in RIPA

Western Blot, densitometer