regulation of ca ++ homeostasis in the hearts of female rats

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Regulation of Ca ++ Homeostasis in the Hearts of Female Rats Sarah-Neïel Barama October 19 th 2006 Supervisor: Dr. Chalifour (LDI)

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Regulation of Ca ++ Homeostasis in the Hearts of Female Rats. Sarah-Ne ïel Barama October 19 th 2006 Supervisor: Dr. Chalifour (LDI). Background & Hypothesis. Relationship b/w ♥ & hormones Ca ++ Homeostasis proteins ( NCX1, SERCA2a, PLB, CSQ, CRT…) Contract = Ca ++ → cytosol - PowerPoint PPT Presentation

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Page 1: Regulation of Ca ++  Homeostasis      in the Hearts of Female Rats

Regulation of Ca++

Homeostasis in the Hearts of Female Rats

Sarah-Neïel Barama

October 19th 2006

Supervisor: Dr. Chalifour (LDI)

Page 2: Regulation of Ca ++  Homeostasis      in the Hearts of Female Rats

Background & Hypothesis Relationship b/w ♥ & hormones Ca++ Homeostasis proteins (NCX1, SERCA2a, PLB, CSQ,

CRT…) Contract = Ca++ → cytosol Relax = Ca++ → SR & ECM

Acute Stress: exercise

Oxidative stress : Doxorubicin ± Dexrazoxane

Hyp: Expression of Ca++ regulating proteins is

. Hormone-dep and is correlated to ♥ fct

Page 3: Regulation of Ca ++  Homeostasis      in the Hearts of Female Rats

Method Intact VS Ovariectomy

Sedentary or Exercised

PBS or DOX or DOX:DEX

♥ Extraction & Homogenisation in RIPA

Western Blot, densitometer