regulation of ca ++ homeostasis in the hearts of female rats
DESCRIPTION
Regulation of Ca ++ Homeostasis in the Hearts of Female Rats. Sarah-Ne ïel Barama October 19 th 2006 Supervisor: Dr. Chalifour (LDI). Background & Hypothesis. Relationship b/w ♥ & hormones Ca ++ Homeostasis proteins ( NCX1, SERCA2a, PLB, CSQ, CRT…) Contract = Ca ++ → cytosol - PowerPoint PPT PresentationTRANSCRIPT
Regulation of Ca++
Homeostasis in the Hearts of Female Rats
Sarah-Neïel Barama
October 19th 2006
Supervisor: Dr. Chalifour (LDI)
Background & Hypothesis Relationship b/w ♥ & hormones Ca++ Homeostasis proteins (NCX1, SERCA2a, PLB, CSQ,
CRT…) Contract = Ca++ → cytosol Relax = Ca++ → SR & ECM
Acute Stress: exercise
Oxidative stress : Doxorubicin ± Dexrazoxane
Hyp: Expression of Ca++ regulating proteins is
. Hormone-dep and is correlated to ♥ fct
Method Intact VS Ovariectomy
Sedentary or Exercised
PBS or DOX or DOX:DEX
♥ Extraction & Homogenisation in RIPA
Western Blot, densitometer