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Detec%onofSubstancesofForensicSignificanceUsingMicrofluidicsand
SERSFunding
Rustin Mirsafavi BMSE
Carl Meinhart Mechanical Engineering
Valentina Hällefors Physics SBCC UCSB
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Real world applications Homeland security, medicine, drug trafficking
Our Research
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The devices Inexpensive and reproducible
Real world applications Homeland security, medicine, drug trafficking
Our Research
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The devices Inexpensive and reproducible
Microfluidics Control small amounts of fluid in a predictable way
Surface-enhanced Raman spectroscopy (SERS) Analyze composition, small sample volume
Real world applications Homeland security, medicine, drug trafficking
Our Research
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This Summer:
• Relate Raman spectra to concentration
Knownconcentra%on
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This Summer:
• Relate Raman spectra to concentration
Knownconcentra%on
• Get Raman spectra for different concentrations
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This Summer
• Relate Raman spectra to concentration
Knownconcentra%on
• Get Raman spectra for different concentrations
• Build devices
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Data Collection
1
2
3
Sample preparation
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Data Collection
1
2
3
Sample preparation
• 0.1-100 µM sample • Flow salt (LiCl) • 20 nm AgNPs
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Data Collection
1
2
3
Microscope & fluidic processes
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Data Collection
• Low velocity creates separated flow
• Mixture of fluids purely driven by diffusion
• Salt induces aggregation of silver necessary for strong signal
Fentanyl
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Data Collection
1
2
3 Laser and SERS
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Data Collection
5 min
• Map channel section with 633 nm laser
• Run device 4-5 times
• Stop after 10-12 minutes 10 min
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Data – Noscapine/Papaverine
(100µM)
(100µM)
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Data – References
Heroin
6-Monoacetylmorphine
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Progress to date
Lab work Microfluidic devices, Raman microscope
Papaverine and noscapine Gathered data for 100uM
Reference spectra Heroin, morphine, 6-MAM
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! Test broader range of concentrations
! Obtain data for other substances
! Spectra-concentration relationship
Future work
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Acknowledgments
Thank You!