drosophila genome how does it differ?. differences drosophila lacks canonical telomeres and the...
Post on 22-Dec-2015
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Drosophila Genome
How does it differ?
Differences
• Drosophila lacks canonical telomeres and the ortholog of vertebrate telomerase.
• Instead it has a unique transposition mechanism.– Two non-LTR (long terminal repeat)
retrotransposable elements, HeT-A and TART telomere-associated retrotransposons are attached to the chromosome ends.
Telomere function
• Prevent the end of the chromosome from being treated as dsDNA break.
• In mammals, loss of telomeres results in cell-cycle arrest and eventual apoptosis.
• In Drosophila, terminal deletions can be recovered and maintained in Drosophila. These ends don’t contain HeT-A or TART sequences thus erode over time, but no cell cycle arrest.– End protection in Drosophila may be sequence
independently mediated by heterochromatin protein HP1.
ALT-alternative telomere maintenance
• Suspected through homologous recombination-telomere-repeat elongation to lengths longer than telomerase creates; larger repeated sequences may be interpersed within telomeres.
• Drosophila-an occational transposition event drives the extension of the telomeric sequences.
Evolution of Transposition
• Loss of sequence dependence of capping• Loss of telomerase• Use of an ALT• Transposable element recruitment to maintain
telomeric activity.
• OR
• Telomerase diverged from transposable elements.
Het-A and TART
How does the retrotransposition take place?
• The colocalization suggests that these two telomeric transposons may have coevolved into symbiotes, with TART supplying the reverse transcriptase and HeT-A the nuclear targeting.
How does the retrotransposition take place?
• For most non-LTR elements, the reverse transcription is primed by a 3' hydroxyl exposed at a nick in chromosomal DNA. Reverse transcription of HeT-A and TART is hypothesized to be primed by the 3' hydroxyl on the extreme end of the chromosome.
Drosophila, worm, mammals
• Size of organism is not correlated with size of genome.
• Smaller worm has 35% more genes 62% more paralogs than flies.
• Half of fly genes have orthologs in mammals, only 1/3 of worm genes has.
Odor receptor genes
• Flies have 57
• Fish has 100
• Mice and worms have 1000.
Transcription factors
• Flies have about 700 (4.5%)
• 500 in worm (3.5%)
Polytene Chromosomes
• Polytene chromosomes are giant chromosomes common to many dipteran (two-winged) flies. They begin as normal chromosomes, but through repeated rounds of DNA replication without any cell division (called endoreplication), they become large, banded chromosomes
Polytene Chromosomes
• Size of each band is an average length of 26.2 kb.
• X chromosome puff- a series of 3.5 (each 350 bp) inverted repeats flaking 154 kb region-these repeats alters the chromosomes macrostructure.