Drosophila melanogaster Staining Lab

Matthew LeeKeenan Mahan

Basic Drosophila melanogaster larva Anatomy

Image courtesy of Morgellons-UK

Adult Fly-C62.8LZ Type

• The lower abdomen of the fly was completely marked with blue stain.

• We believe that this shows the neural pathways within the leg muscles of the fly.

• The diagram next to our images shows the muscle development that occurs within Drosophila.

Adult Fly-C62.8LZ Type

Image courtesy of PhysOrg.com

Larva-C62.8LZ Type

• In adult flies the C62.8LZ Type had highlighted regions in the abdomen that linked to the legs at one point.

• In the larva the C62.8LZ has the stain in areas where the legs begin to develop as well as in what appears to be a neural pathway from the head of the larva to the anus.

• The C62.8LZ Type is stained mostly in motor neuron pathways in the legs and in some other areas of the body in both the adult fly and larva.

Larva-C62.8LZ Type

Image courtesy of Dr. Brian

Staveley

Larva-en Lac Z Type

• For en Lac Z Type larva the midintenstine, or midgut, was the part most stained.

• When compared to the picture on the right of our pictures one can see how in both pictures the larva’s intestines are exposed and stained a blue/purple color.

Larva-en Lac Z Type

Image courtesy of Indranil Mukhopadhyay, Daya Krishna Saxena,

and Debapratim Kar Chowdhuri

Larva-C362.14 UAS1 Type

• The part of the larva stained for this type appears to be the salivary glands.

• On each side of the larva there is a sac that is clearly stained in blue, and that sac is located at the same region as the salivary glands in Drosophila.

• When compared to the highlighted salivary glands in the three larvae in the picture on the right, it appears as though salivary glands are clearly stained in both pictures.

Larva-C362.14 UAS1 Type

Image courtesy of TriTech Research

Image Sources• "BIOL3530: Developmental Biology, Drosophila Development." Memorial University. Web.

07 Dec. 2010. <http://www.mun.ca/biology/desmid/brian/BIOL3530/DB_02/DBNDros.html>.

• "Close Associations: Micro-Myiasis & Morgellons Disease." Morgellons UK - Campaign 2010 - Raising Awareness to the Public and Professionals. Web. 07 Dec. 2010. <http://www.morgellonsuk.org.uk/micromyiasis.htm>.

• "Drosophila Vials, Bottles, Incubators, Fly Pads, CO2 Anasthesia Stations and Other Products - Tritech Research, Inc." Specialists in Microinjectors, Incubators and Electroporators - Tritech Research, Inc. Web. 07 Dec. 2010. <http://www.tritechresearch.com/dros.html>.

• Mukhopadhyay, Indranil, Daya Krishna Saxena, and Debapratim Kar Chowdhuri. "Hazardous Effects of Effluent from the Chrome Plating Industry: 70 KDa Heat Shock Protein Expression as a Marker of Cellular Damage in Transgenic Drosophila Melanogaster (hsp70-lacZ)." EHP Online: Home of Environmental Health Perspectives. Web. 07 Dec. 2010. <http://ehpnet1.niehs.nih.gov/members/2003/6339/6339.html>.

• Staveley, Brian. "Fruit Flies -- A Model for Bodybuilders." PhysOrg.com - Science News, Technology, Physics, Nanotechnology, Space Science, Earth Science, Medicine. Web. 07 Dec. 2010. <http://www.physorg.com/news187540044.html>.