emma l. aronson allison lab uc irvine may 17, 2012
DESCRIPTION
Impact of varied precipitation and N deposition on grassland soil microbial communities and GHG flux. Emma L. Aronson Allison lab UC Irvine May 17, 2012. Precipitation hypotheses. Control. Increased Rainfall. Decreased Rainfall. - PowerPoint PPT PresentationTRANSCRIPT
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Impact of varied precipitation and N deposition on grassland soil
microbial communities and GHG flux
Emma L. AronsonAllison labUC Irvine
May 17, 2012
![Page 3: Emma L. Aronson Allison lab UC Irvine May 17, 2012](https://reader035.vdocument.in/reader035/viewer/2022062517/56812eed550346895d94897f/html5/thumbnails/3.jpg)
Precipitation hypotheses
Indicates level of soil bacterial biodiversity
Decreased Rainfall Control Increased Rainfall
Indicates rate of CO2 and N2O release, CH4 consumption
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N amendment hypotheses
Control
Indicates level of soil bacterial biodiversity
Indicates rate of CO2 and N2O release, CH4 consumption
N Added
N N N N N N
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In March, N and H2O- increased CH4 uptake
production
uptake
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H2O- increased CH4 uptake
production
uptake
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N increased CH4 uptake
production
uptake
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Rain decreased, then increased uptakeproduction
uptake
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CH4 flux correlates with initial [CH4]
500 1000 1500 2000 2500 3000 3500
80
60
40
20
0
-20
-40
-60
-80
CH
4 F
lux
(ppb
min
-1)
production
uptake
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H2O- decreased respiration
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Time interacts with precipitation
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Time interacts with precipitation
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Rainfall increases respiration
Courtesy of Greg Winston
CO2 F
lux
(mg
m-2
h-1
) Precipitation (in d-1)
Day of Study
Precipitation
CO2 Flux
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N increases N2O release
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Time interacts with N treatment
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Time interacts with N treatment
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Rain Effect?
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Drought changed bacterial community
Ambient Rainfall Reduced Rainfall
Significantly Different Phyla
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Reduced rainfall decreases variability in microbial richness at OTU level
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Drought decreased respiration and variability in bacterial diversity, increased CH4 uptake
Decreased Rainfall Control Increased Rainfall
Indicates rate of CO2 release, CH4 consumption
microbial community variability
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N amendment increased N2O release, CH4 uptake
N Added Control
N N N N N N
Indicates rate of N2O release, CH4 consumption
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Research in progress
• Further 16S 454 sequencing – for all dates of gas analysis
• Shotgun metagenomic DNA sequencing • GeoChip functional microarrays or qPCR• Gene expression of soil N and C cycling
functions
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Questions?Questions?