enzymatic synthesis of valuable compounds using microbial

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Enzymatic Synthesis of Valuable Compounds Using Microbial Cells Step 1: Isolation and selection of microorganisms · Screening of target enzyme · Evaluation of enzyme activity · Enhancement of the activity Step 2: Enzyme characterization and gene cloning Natural environment Pure cultures Functional analysis Structural analysis · Analyses of enzyme’s function and structure · Enzyme engineering · Construction of recombinant microbe Genetic recombination Isolation of microorganisms Enzyme purification Gene cloning Step 3: Enzymatic production of valuable compounds Microbial cells of wild strain or recombinant Raw materials Enzymatic conversion Valuable products · Enzymatic synthesis under mild conditions without by-products · Environmentally friendly system Over-expressing cells

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Page 1: Enzymatic Synthesis of Valuable Compounds Using Microbial

Enzymatic Synthesis of Valuable Compounds Using Microbial CellsStep 1: Isolation and selection of microorganisms

· Screening of target enzyme· Evaluation of enzyme activity· Enhancement of the activity

Step 2: Enzyme characterization and gene cloning

Natural environment

Purecultures

Functional analysis Structural analysis

· Analyses of enzyme’s function and structure· Enzyme engineering· Construction of recombinant microbe

Geneticrecombination

Isolation ofmicroorganisms

Enzyme purification

Genecloning

Step 3: Enzymatic production of valuable compoundsMicrobial cellsof wild strainor recombinant

Rawmaterials

Enzymatic conversion

Valuableproducts

· Enzymatic synthesis under mild conditions　　　　　without by-products· Environmentally friendly system

Over-expressing cells

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