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Enzymes In Depth With pH!

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Page 1: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

Enzymes In Depth

With pH!

Page 2: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

pH

Page 3: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both
Page 4: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both
Page 5: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both
Page 6: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

Enzymes

• Globular proteins• Built from amino

acids that have acidic and basic characteristics– Amino acids have

both a carboxylic acid (COOH) and amine (NH3) groups

Page 7: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

Enzymes

• R-COOH C-COO -1 + H+1• R-NH3 +1 R-NH2 + H+1

• Ionic bonds• Increase solution pH can shift equilibrium

Page 8: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

• Active sites have R groups• R groups help substrates attach to active site• Only works if the arrangement of R groups

interact properly

Page 9: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

• +/-• H = Hydrogen bonding• R = Van der Waals bonding

Page 10: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

At optimal pH• Enzyme attaches to substrate with 2 ionic

bonds • Groups allow for ionic bonding (caused by a

transfer of H ion from COOH in the side chain of one amino acid to a NH2 group

Page 11: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

Acidic pH

• Nothing will bother the NH3 + but the COO will want a H ion– pH (acid = more H ions; basic = more OH ions)

• No ionic bond can form

Page 12: Enzymes In Depth With pH!. pH Enzymes Globular proteins Built from amino acids that have acidic and basic characteristics – Amino acids have both

Basic pH

• COO- will not be affected• But NH3+ will lose a H• No ionic bond will form


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