NRL-Salmonella The Netherlands Kirsten Mooijman

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Dutch NRL-Salmonella at RIVM/Z&O ● Situated at the Centre for Zoonoses and Environmental

Microbiology (Z&O: approx. 60 staff members) ● Z&O is part of the National Institute for Public Health and

the Environment (RIVM), Bilthoven, the Netherlands (approx. 1500 employees)

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Organogram RIVM

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Organogram Z&O

Departments

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Biological NRLs at RIVM-Z&O

● Salmonella (since ca 1993) ● Parasites (since ca 2005) ● Bacteriological and viral contaminants of bivalve

molluscs (since ca 2005) ● Escherichia coli, including VTEC (since 2011) ● Listeria monocytogenes (since 2011) ● Coagulase pos Staphylococci, including Staphyloccocus

aureus (since 2011)

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Tasks and duties NRLs

Defined in European legislation: For food and feed in: ‘Regulation (EC) 882/2004 on official

control performed to ensure the verification of compliance with feed and food law, animal health and animal welfare rules’ (April 2004)

changed to Regulation (EU) 2017/625 (March 2017)

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Tasks and duties of NRLs

● Collaborate with EURL in relevant area ● Coordinate activities of official national

laboratories for analysis of samples ● Organise comparative tests between official

national laboratories, including follow-up ● Disseminate information supplied by the EURLs

to authorities and national laboratories ● Provide scientific and technical assistance to the

national competent authority ● Quality system accredited according to ISO

17025 in relevant workfield

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‘Collaborate with EURL in relevant area’

● EURL and NRL for Salmonella situated at the same institute

● Important to separate activities for EURL and for NRL, especially for interlaboratory studies of the EURL. The technician who will participate in the interlaboratory study as NRL will receive blind samples, similar as for the other NRLs.

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‘Coordinate activities of official national laboratories for analysis of samples’

● NRL does not perform sample analysis for monitoring programmes; this is done by the official laboratories

● NRL gives advices/ visits/ trainings for official labs when needed

● NRL performs confirmation/ additional typing, e.g.: – Occasionally analyse samples for second opinion – (sero)typing of Salmonella isolates which official laboratories

are not able to type to serovar level – Confirmation of isolates typed as ‘monophasic Salmonella

Typhimurium’ by official laboratories

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‘Organise comparative tests’

● For analysis of Salmonella in food samples only one official national laboratory in NL: Netherlands Food and Consumer Product Safety Authority (NVWA). Both NRL and NVWA participate in PT (food) schemes of EURL.

● For analysis of Salmonella in samples from primary production stage (pps) and serotyping of isolates from these samples NL has 22 officially recognised (private) laboratories, of which 13 also perform serotyping of Salmonella.

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Organisation comparative tests for pps ● Up to approx. 2009, NRL organised PT schemes itself (one

study per year for detection of Salmonella pps). Since 2009 the ministry did not longer offered budget for organisation PT schemes for commercial (private) laboratories.

● Discussion of options with ministry and official laboratories (OLs): a) continue organising PTs by NRL and ask fee from OLs, or b) select a commercial PT scheme in which all OLs have to

participate (for own costs) ● Agreed to go for option b) (less expensive than option a.).

Labcodes of each OL have been forwarded to the NRL so that performance in PT schemes of each lab can be checked by NRL; NRL-Salmonella participates in the same PTs.

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Comparative tests ● Prescribed PT schemes: VetQas of APHA, UK; accredited by

UKAS: – PT 0088: Salmonella in poultry – PT 0084: Salmonella serotyping – Each study: 4x 5 freeze dried samples per year

● Together with competent authority agreement on judgement of results of official laboratories: – Per study, per lab:

› 5 samples okay: good; 4 okay: moderate; ≤ 3 okay: unsatisfactory

– Trend analysis of successive studies, per lab: › Trend should (at least) be (over 4 studies): unsatisfactory moderate moderate good

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Examples summarised results per PT scheme

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Samenvatting Salmonella detectie ringtest augustus 2016

Monsteroverzichtnummer pos / neg serotype (facultatief) en overige info16/1628 +ve Salmonella Typhimurium (group B; > 1E5 cfu/vial) + Citrobacter youngae16/1629 +ve Salmonella Enteritidis (group D; approx. 30 000 cfu/vial) + Pseudomonas aeruginosa16/1630 -ve Citrobacter youngae16/1631 +ve Salmonella Typhimurium monophasic (group B; approx. 10 000 cfu/vial) + E. coli16/1632 +ve Salmonella Kedougou (group G; approx. 50 cfu/vial)

Aug 2016Code # correct opmerkingen1076 - Geen deelname1115 51121 51158 51235 51387 51436 5 16/1628 en 16/1631 afgegeven als monofasisch S. Typhimurium ipv S. Typhimurium1559 51647 51673 51770 51947 52096 52111 4 Monsters detectiestudie en typeringsstudie verwisseld. Resultaten in typeringsstudie

voldoen aan resultaten detectiestudie, inclusief serotypering.2143 52158 - Geen deelname2204 52212 52229 5 16/1631 afgegeven als 'other' ipv S. Typhimurium2240 52274 52426 52472 - Nil return2904 51948 5

Rood: onvoldoendeBlauw: matig

opmerking

Samenvatting Salmonella serotypering ringtest mei 2016

Monsteroverzichtnummer antigenen groep serovar16/1370 1,4,[5],12:i:1,2 B S. Typhimurium16/1371 1,6,14,25:y:e,n,x H S. Fischerkietz16/1372 1,13,23:f,g,[s]:- G S. Havana16/1373 1,4,[5],12:i:- B monofasisch S. Typhimurium16/1374 1,13,23:i:l,w G S. Kedougou

Mei 2016Code # OK VetQas # OK NVWA Opmerkingen:1115 5 51121 2 51158 5 51436 5 51559 2 51647 - - Geen deelname1947 2 52111 2 52143 5 52212 2 5 Nil return, maar NRL geïnformeerd en ruwe data toegestuurd.2229 1

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16/1373 afgegeven als 'other', terwijl monofasisch S. Typhimurium onderdeel is van erkende serotypes; 16/1374 onterecht afgegeven als monofasisch S. Typhimurium.

2240 2 4 16/1373 onterecht afgegeven als S. Gloucester.2274 1 4 16/1373 niet volledig uit getypeerd (afgegeven als groep B), terwijl

monofasisch S. Typhimurium onderdeel is van erkende serotypes.2426 2 5

Rood: onvoldoendeBlauw: matigGeel: attendering

Example summarised results, trends in PT schemes

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Results comparative tests

● In case of unsatisfactory results in one study NRL contacts OL: explanation for poor results? Advices needed?

● In case of unsatisfactory trend results of several successive PTs NRL contacts OL and Competent Authority (CA).

● CA can decide to (temporary) suspend the recogniction of an official laboratory.

● Actions for improvement: e.g. training at NRL, audit by NRL

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‘Disseminate information supplied by EURL to authorities and national laboratories’

● EURL-Salmonella reports are forwarded to Competent Authority

● Newsletters EURL-Salmonella are also sent to Competent Authority

● Technical information from EURL-Salmonella forwarded to official laboratories

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‘Assist the national competent authority’

● Cooperate with Netherlands Food and Consumer Product Safety Authority (NVWA), e.g. for recognition of official laboratories

● Discuss/ give advice for diagnostics and monitoring in relation to national programmes

● Discuss/ give advice on new/amended EC legislations ● Participate in committees at national level ● Assist in case of outbreaks

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‘Quality system accredited according to ISO 17025 in relevant workfield’

● RIVM-Z&O accredited since 2005; annual visit by the Dutch Accreditation Council

● Since 2005 scope has been enlarged until currently 12 activities

● For NRL-Salmonella (and EURL): detection of Salmonella in food, animal feed and pps conform EN ISO 6579:2002 and Annex D: 2007. Next year conform EN ISO 6579-1

● Registration under: www.rva.nl L421

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Thank you for your attention! Questions?

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