experience with results
TRANSCRIPT
![Page 1: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/1.jpg)
Kristy D. Wendt
Material Surface Characterization: Partnership Building and Characterization Experience
![Page 2: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/2.jpg)
Nano Research Facility Core Laboratories
Fabrication Laboratory
Particle Technology Laboratory
Surface Characterization Laboratory
Bio-Imaging Laboratory
FEI NovaTM 230 FE NanoSEM (EDS) Elemental Mapping
FEI TecnaiTM Spirit TEM
Leica UC7 Ultramicrotome
Veeco NanomanTM AFM
Zeiss LSM 710 Confocal Microscope
![Page 3: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/3.jpg)
Starting On Campus: Training Protocol Development EM Core Facility Collaboration
![Page 4: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/4.jpg)
Image and Technique Portfolio: Image Processing FEI Tecnai SpiritTM Quantifying Size Dispersion
Client: Dr. Younan Xia, Washington University
Sample: ”Bottom up” shape controlled synthesis silver and gold nanoparticles
Expectations: Quantification of size dispersion
500 nm 500 nm
TEM of ~200 nm Ag Nanocubes Binary Image with Global Threshold
![Page 5: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/5.jpg)
Client: Dr. Brent Riggs, NRF
Sample: Photolithography, Fabrication
Expectations: Measure the thickness, density, and composition of films, identify profile shape, identify contamination and trace impurities
Image and Technique Portfolio: FEI NovaNanoTM SEM Finding the Right Angle
![Page 6: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/6.jpg)
Image and Technique Portfolio: FEI NovaNanoTM SEM Troubleshooting Difficult Samples
Client: Dr. Pratim Biswas, Washington University
Sample: TiO2 crystal films
Expectations: Difficult to image sample, high-contrast/charging
Solution: Sputter coating current, reduced voltage, immersion mode, increased WD
![Page 7: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/7.jpg)
Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Surface Characterization
Client: Dr. Akira Ohnuma, Washington University
Sample: Asymmetric hybrid colloidal particles by precipitation polymerization
Expectations: Characterize a surface modified polystyrene bead
![Page 8: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/8.jpg)
Image and Technique Portfolio: FEI NovaNanoTM SEM Murine Kidney Glomerulus
Client: Dr. Jeff Miner, Washington University
Sample: Kidney organogenesis in murine models
Expectations: Processing protocol development, basement membrane preservation
![Page 9: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/9.jpg)
Image and Technique Portfolio: FEI NovaNanoTM SEM Aluminum Polarization Filters
Client: Dr. Victor Gruev, Washington University
Representative Research Area: Nanofabrication of aluminum polarization filters
Expectations: Side wall analysis
![Page 10: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/10.jpg)
Image and Technique Portfolio: FEI Tecnai SpiritTM TEM Instrument Limit
Client: Dr. Byungkwon Lim, Washington University
Sample: Palladium-platinum bimetallic nanocrystals
Expectations: Ultrahigh magnification, show dendritic morphology; enhanced activity toward oxygen reduction in the fuel cell industry.
![Page 11: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/11.jpg)
Image and Technique Portfolio: Efficient Sessions for Large Group Projects
Client: Dr. Kathy Hafer, Washington University
Sample: lambda phage
Expectations: Provide large scale learning experiment, including processing and imaging for lambda phage
![Page 12: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/12.jpg)
Image and Technique Portfolio: Nanotoxicity in Yeast
Client: Dr. Yujie Xiong, Mark Brunson (REU) Washington University
Sample: Saccharomyces cerevisiae
Expectations: Processing protocol development for saccharomyces cerevisiae
![Page 13: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/13.jpg)
Image and Technique Portfolio: FEI NovaNanoTM SEM Silica Chips on Silicon Substrate
Client: Dr. Lan Yang, Lina He Washington University
Sample: Silica microresinators on silicon substrates
Expectations: Lower contrast image on difficult sample, ETD, longer working distance
![Page 14: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/14.jpg)
Image and Technique Portfolio: Qualitative Analysis by EDS Elemental Mapping/Overlay
Secondary Electrons Platinum Overlay 1 µm
Tin balls Overlay
![Page 15: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/15.jpg)
Image and Technique Portfolio: Line Mapping Ag and Pt Nanoparticles
Silver Nanoparticles on Carbon
Gold on Carbon Platinum
![Page 16: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/16.jpg)
Image and Technique Portfolio: Nanoskiving
Conventional Top Down Approach
Useful Versatile Highly Developed
High capital Operating costs No non-planar surfaces Material class restrictions
![Page 17: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/17.jpg)
Conventional to Unconventional Techniques: Conventional Processing of Biological Tissue
High Contrast -Osmium tetroxide -Lead Citrate -Uranyl Acetate
Thin -Resin Infiltration -Thermal Polymerization -Section 65 nm
Dry -Ethanol Gradient
Fixation
![Page 18: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/18.jpg)
Image and Technique Portfolio: Nanoskiving
PDMS EPOXY
EPOXY
PDMS
EPOXY
Cast and cure epoxy
Separate epoxy
EPOXY
Deposit metal
Embed in epoxy and cure
EPOXY
Ultramicrotomy
Thin-section ~70nm
Knife blade
![Page 19: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/19.jpg)
Image and Technique Portfolio: Nanoskiving
Epoxy
Au on epoxy
Epoxy
PDMS
Knife
Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577
![Page 20: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/20.jpg)
Image and Technique Portfolio: Nanoskiving
Xu Q et al. Acc. Chem. Res., 2008, 41 (12), pp 1566–1577
1. Nanostructure manipulation 2. 3D Stacking 3. No clean room
![Page 21: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/21.jpg)
Collaborating with Nanoscale Informal Science Education Network Participating in WU’s Educational Research Group Organizing and Hosting Public Outreach Festivals at WU
![Page 22: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/22.jpg)
Developing Outreach: Nanodays at the Saint Louis Science Museum
![Page 23: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/23.jpg)
Managing Research Experience for Undergraduates (REU) Program
![Page 24: Experience with Results](https://reader035.vdocument.in/reader035/viewer/2022062419/55a37da51a28abfb158b46bc/html5/thumbnails/24.jpg)
Kristy D. Wendt
-Broad range of material and biological EM processing -Enjoys addressing unique challenges
-Novel protocol development -Fabrication and particle analysis -Training and partnership building
-Works well with a broad range of clients to get beautiful results