ITEM NO. 22 COPY NO.FILE NO. XXVIH-33

EXPERIMENTAL PRODUCTION OF PENICILLINAT THE ST. JACOB’S HOSPITAL, LEIPZIG

COMBINED INTELLIGENCE OBJECTIVESSUB-COMMITTEE

Report on

EXFER IIvENTiJL PRODUCT JON CF PENICILLIN AT THE sT. JACOB'SHOSPITkL, LEIPZIG

Reported by t

jMt. P.L, PAVCEK, ToI.I.C

CIOS Target No. 22/1289Food and Agriculture

COMBINED INTELLIGENCE OBJECTIVES) SUB-COMMITTEEG-2 Division, SHAEF (Rear) APO 1+ 13

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Department of Dermatology.

Dr# Joseph Kimmig started research on penicillinudder Prof. Joseph von Kennel in Kiel in 1942. In 1943the firm of Sobering A.G., supporting the work, trans-ferred the project to St. Jacob’s Hospital where it hasbeen in progress ever since. The Hospital is a very newbuilding and has beautiful laboratories and equipment.

penicillin was being.grown on a rather smallscale in one of the laboratories in bottles containing300 ccs. of Chopeh-D oz medium. No other adjuncts wereadded. The mold, of which a culture was obtained, wasisolated by Kimmig from the flower of Blue Berries andwas identified as P. Chryseum flavum. ns high as 40 unitsof Penicillin per cc. were produced in a ten day incuba-tion at 26°C. but for continuous operation the figure was10-15 units/cc,

A special procedure was adopted to build up theinoculum. The spore suspension gorwn on a standardnutrient Kuczynski agar (sample obtained) was diluted andplated out until h culture originating from 1 spore wasobtained. 12-15 plates of such spore cultures wereinvestigated separately for ability to produce penicillin,and in this manner the most potent culture was selected.

For testing the unitage in the final solution,1 cc. of the test solution was inoculated with 3. aureusincubated at 37° for 24 hours. Dilutions were maaeaccording to the attached protocol (Table 1).

The total weekly capacity of the laboratory set-upwas 200,000 units. Some experiments had been conductedwith submerged cultures but maximum unitage obtained wasl/4 Unit/oc. Kimmig felt that this culture was notsuited to this type bf culturing.

Other workers on Penicillin in Germany included;■Bernhauer in Prague, R. Kuhn (Heidelberg) and the firmSchatt & Genossen (Jena).

Dr. Kimmig demonstrated a new observation oninhibition of oxygen uptake by S. aureus in the presenceof Penicillin or malonic acid. On a Ringer phosphatemedium 1:10,000 malonic acid produced the same inhibition

2

as did Penicillin. According to Kimuiig the effect ofPenicillin may be due to a disturbance in the succinicacid cycle.

Dr. Hermann Fox also associated with SoberingA.G-* had done research on sulfonamides.

A sulfonamide like compound had been produced byI.G-. Farben (Leverkusen) and had very powerful bactericidaleffects. The compound, tetra-brom#-benzil, had not beenclinically tested as yet.

Several reprints from Kimmig were obtained. Oneon the effect of para amino benzo acid in preventing sunburnwas especially interesting.

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