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ACQUITY UPLC I-CLASS

ACQUITY UPLC H-CLASS

nanoACQUITY UPLC

PATROL UPLC

From routine to research, always the right answers.

The analytical laboratory has come a long way. For years, while the world of technology took great leaps forward, analytical chemistry lagged behind. hPlc couldn’t keep pace with the urgency of business demands to analyze more samples, faster, and with better results.

CHANGE STARTED HERECHANGE STARTED HERE

Our ACQUITY UPLC Systems are more than innovative. They are proven. scientists worldwide have used uPlc to eliminate laboratory bottlenecks – with one acQuity uPlc system typically displacing multiple hPlc instruments. with more work being accomplished on fewer instruments, acQuity uPlc is also saving energy, using less solvent, and producing less waste. uPlc is driving a wide range of scientific achievements, from standard laboratory testing to breakthrough discoveries.

CHANGE STARTED HERECHANGE STARTED HERE

at waters, we saw the need for change – and responded with the invention of an entirely new category of chromatographic performance. Leveraging what we learned from hundreds of customer conversations, we pioneered holistic innovation that simultaneously addressed lc particle technology and instrument design. we engineered advanced fluidics modules in tandem with sub-2-µm hybrid particle columns to deliver superior performance at higher pressures. system volumes were minimized. Flow paths, optimized. and the result was ultraPerformance lc,® better known today as uPlc.®

today, waters provides an ever-growing family of acQuity uPlc® systems with one goal in mind – delivering improvements in analytical resolution and sensitivity that give you the confidence that your scientific, operational, sustainability, and profitability goals can be met. our systems, chemistries, software, and services are optimized for your specific applications to efficiently deliver more information per unit of work. so that every laboratory can leverage the very best in analytical performance.

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TRANSFORMING LIVES AND LABORATORIESTRANSFORMING LIVES AND LABORATORIES

TRANSFORMING LIVES AND LABORATORIES

And today, UPLC technology is transforming lives and laboratories, creating new opportunities for business profitability, and bringing new meaning to quality.

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TRANSFORMING LIVES AND LABORATORIES

Because your benchmark is beyond the bench.

ACQUITY UPLC’s impact goes far beyond a

laboratory report. It reaches our everyday lives.

UPLC resolution and sensitivity enables scientists

to identify trace contaminants in water, grain, meat,

and milk – up and down the food chain. It sets new

benchmarks in every laboratory, whether your goal is to

discover and identify novel pharmaceutical ingredients

or to have more confidence in developing drug

therapies. Scientists can now understand more about a

sample than ever before. They can produce more reliable

and reproducible information. And, in the end, bring

about a safer and better world.

BEYOND THE BENCHBEYOND THE BENCH

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ACQUITY UPLC I-CLASS

since its invention, acQuity uPlc technology has enabled analytical chemists to reach far beyond conventional hPlc separations. acQuity uPlc set new standards in resolution, sensitivity, and throughput by being the first holistically-designed system that maximizes the performance of sub-2-μm particles. the acQuity uPlc i-class system is designed for complex analyses: delivering the highest peak capacity, the system keeps pace with advances in mass spectrometry technologies.

ACQUITY UPLC I-Class is the ultimate separations tool, providing more power to resolve even highly complex and dilute samples. you gain more information about your sample in a single run than ever possible before.

Where UPLC inherently improves MS sensitivity, the acQuity uPlc i-class system provides such advances in peak definition and concentration that ionization capabilities will take another leap forward. For triple quadrupole ms quantitation, new limits of detection and sensitivity are within reach for compounds with high efficacy and low dosage; for qualitative time-of-flight ms, more signal scan is available for accurate and exact mass identification and quantification of all components within a compound.

Laboratories are making tremendous upgrades in productivity with uPlc. with a full suite of acQuity uPlc columns in five particle substrates and 20 chemistries, and the versatility to analyze a single sample or automate runs in the thousands, it’s ready for your application.

Improves laboratory efficiency by optimizing instrument utilization; on average one uPlc system displaces three hPlc instruments.

Proven to be the most valuable analytical asset in laboratories around the world, uPlc has fueled more than 500 peer-reviewed papers, and has served the needs of regulatory agencies around the globe.

Reduces solvent consumption up to 95%, for a greener laboratory.

BEYOND THE BENCHBEYOND THE BENCH100

0

MRM of 1 Channel ES+346.083 > 198.068 (Omeprazole)

7.06e7

MRM of 1 Channel ES+346.083 > 198.068 (Omeprazole)

4.42e3

Omeprazole 10 ng 0403

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The ACQUITY UPLC I-Class System is an ideal match for mass spectrometry. Here, the system was paired with a Xevo® TQ-S for the analysis of omeprazole, achieving a quantification range of 500 ag to 10 pg on-column. No detectable carryover within 20% of the limit of quantitation was observed after the injection of the highest concentration standard.

The low dispersion characteristics of the ACQUITY UPLC I-Class System allow you to push your separations to a new level. This ballistic gradient for a series of anesthetics achieved standard deviations for retention time as low as 12 ms for six replicates. The separation achieved a peak capacity of 46 in 10 s with a 1/2 height peak width as low as 88 ms.

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System: ACQUITY UPLC I-Class (SM-FL)Column: 2.1 x 30 mm BEH C18 1.7 µmTemperature: 90 °CFlow rate: 2.0 mL/minMobile phase A: 10 mM ammonium bicarbonate pH 10Mobile phase B: AcetonitrileGradient: 35% to 95% B over 10 s (2 s pre-inject delay)Data rate: 80 Hz

ACQUITY UPLC H-CLASS

if you’re an hPlc user, now you can take advantage of uPlc for routine analytical tasks. the acQuity uPlc h-class system is specifically designed to accelerate method development and allow for seamless method transfer to and from any hPlc. you will achieve significantly better resolution and improved throughput compared to traditional hPlc, without sacrificing sensitivity and precision. acQuity uPlc h-class works like hPlc – but performs like uPlc. uPlc is now accessible to everyone.

From a single analytical instrument to hundreds of screening systems, from development through Qa/Qc, the acQuity uPlc h-class system is a scalable solution that keeps up with your organization as it changes and grows.

With a low-dispersion quaternary solvent delivery system, the acQuity uPlc h-class delivers pulse-free gradients resulting in the most precise and flexible eluent composition for your analytes.

Now the standard in hundreds of laboratories, our quaternary system has been adapted for specific applications. the acQuity uPlc h-class Bio system features a bio-inert flow path and our patented Auto•Blend Plus™ Technology, which are perfectly suited to challenging biopharmaceutical applications such as ion exchange (ieX) and size exclusion (sec) chromatography. the acQuity uPlc h-class method Development system incorporates specialized Quality by Design (QbD) software that automates a statistical approach to determining a fully-optimized method.

Pre-configured method development and method transfer column kits ensure hPlc and uPlc methods can be developed and transferred with ease.

The ACQUITY UPLC H-Class System is proven to significantly improve laboratory productivity, enabling faster time-to-market, increasing profitability by reducing manufacturing costs, all while improving the quality of information produced.

CONFIDENCECONFIDENCE

The innovations of UPLC are now within reach

for chemists who go to work to keep business

moving as usual.

You know that your colleagues in labs

upstream and downstream depend on you,

and that a single peak confirms all is well.

Too many peaks in a separation make people

start to worry. With the ACQUITY UPLC

H-Class System, you’ll get through more

samples faster. You’ll know earlier if the

analysis is not right – and you’ll have the data

that indicates why. There’s more confidence in

QA, QC, and in any lab where routine really

means business-critical. And those other labs?

Sure, you can transfer your methods.

You’ve got ACQUITY UPLC H-Class.

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Solvent composition is easily adjusted using the Auto•Blend™ functions of the ACQUITY UPLC H-Class System. In this example, the optimum concentration of TFA for a peptide map is identified simply by varying the percentage of flow taken from the D line. There is no need to make extra bottles of solvent, and intermediate values can be tested with minimal effort.

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0.025% TFA – 2.5% D

0.050% TFA – 5.0% D

0.100% TFA – 10.0% D

Enolase Peptide Digestion StandardColumn: Peptide Separation Technology BEH300 C18, 2.1 x 100 mm, 1.7 µmSolvents: A: Water, B: Acetonitrile, C: Isopropanol, D: 1% TFA

CONFIDENCECONFIDENCE

nanoACQUITY UPLC

when it comes to the most challenging applications, you no longer have limits on performance. labs on the forefront of discovery often have very small amounts of complex, irreplaceable samples and are challenged to quantify small changes over several orders of magnitude. the nanoacQuity uPlc® system optimizes all the scientific advantages of uPlc, so you can trust it to analyze the most precious specimen.

Direct nano-flow design enables you to attain the highest levels of chromatographic resolution, sensitivity, and reproducibility that are fundamental for reliable qualitative and quantitative proteomics studies.

Designed for microbore- to nano-scale separations, nanoacQuity uPlc yields higher peak capacity and peak shape without flow-splitting, and increases the number of molecules that can be detected per separation.

Available in two-dimensional (2D) UPLC to push the boundaries of chromatographic resolution of complex proteomic samples. its proprietary online, dual-reversed-phase design provides a new approach to orthogonal separations.

industry-leading inlet to mass spectrometry demonstrates improved sensitivity, spectral quality, and productivity. nanoacQuity uPlc provides flexible instrument method editing and sample set execution for any mass spectrometer.

DREAM MACHINEDREAM MACHINE

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65.0% Acetonitrile

20.8% Acetonitrile

17.4% Acetonitrile

14.5% Acetonitrile

11.1% Acetonitrile

See what scientific dreams are made of.

Pair nano-scale UPLC with top-of-the-line High

Definition Mass Spectrometry™ (HDMS™)

to analyze disease states molecule by molecule.

You’ll fully explore all protein characteristics.

And see your ideas become reality. Waters works

hand-in-hand with world-leading laboratories

in academia, biotechnology, and pharmaceutical

companies to ensure that UPLC/MS/MS technology

and software provide the tools and techniques that

enable you to perform the most challenging scientific

tasks. nanoACQUITY UPLC is focused on your

analytical needs, so that you can focus on pure

science. After all, the possibility that the cures

for cancer, autism, or Alzheimer’s might start

with your discovery is a dream we also believe in.

High-peak-capacity UPLC separations of rat brain cellular lysate using the proprietary RP/RP approach of the nanoACQUITY UPLC 2D System.

DREAM MACHINEDREAM MACHINE

Quality will always have a market.

In the world marketplace, a product’s

reputation is built on quality. When UPLC is

used on the manufacturing floor, it provides

companies with the confidence to ensure

that a product – from biopharmaceuticals, to

cosmetics, to beverages – will stand up to its

promise of quality. This translates directly to

consumer confidence and product success.

brand GUardIanbrand GUardIan

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Mapping the progress of an API and its impurities in a manufacturing reaction vessel

with the PATROL UPLC System provides a better understanding of the reaction’s progress, so you can optimize product yield and purity.

brand GUardIanbrand GUardIan

PATROL UPLC

the real-time process analytical technology (Pat) system, the Patrol uPlc® Process analyzer system analyzes and quantifies complex multi-component manufacturing samples and final product directly on the production floor for maximum production efficiency. the Patrol uPlc laboratory analyzer system is designed for reaction monitoring and optimization experiments. It integrates sample prep and Real-TIME LC™ analysis to automate fast and accurate quantitative results that define a process method.

Applies Quality by Design data and metrics directly to the manufacturing process.

UPLC methods can be directly transferred from the process development laboratory to the manufacturing floor, for live feedback supported by historical reaction characterization data.

Enables a comprehensive understanding of process efficiency and quality.

Assures end-product quality, including final release testing.

Reduces process cycle times and waste, so more product can be produced with existing resources.

Integrates Waters’ best-in-class technologies, delivering the right information at the right time in a system optimized for continuous uptime.

ALL SYSTEMS GOALL SYSTEMS GOUPLC Technology – ready to grow with you.

acQuity uPlc systems can dramatically transform your laboratory’s capabilities and your organization’s accomplishments.

uPlc techniques refined on limited sample amounts can more easily pass forward to biomarker identification to determine the potential for therapeutic success.

Quality testing protocols for commercial products can readily be shared by global organizations to achieve localized regulatory standards, no matter what country an end product is shipped to.

acQuity uPlc methods will seamlessly transfer from development through quality control and assurance and on to manufacturing – enabling process efficiencies such as just-in-time manufacturing.

ACQUITY UPLC I-Class System

ACQUITY UPLC H-Class System

thousands of our customers worldwide are already reaping business benefits that they can measure against their investment in UPLC technology. Our systems continue to evolve, and are ready to grow with you.

Waters software – ready to turn results into knowledge. waters informatics integrate all of your lab resources – for a consistent, scalable, and seamless application environment. uPlc can be driven with either the industry-leading empower® software for instrument control and data management, masslynx® software for advanced mass spectrometry applications, or our next-generation platform, the uniFi® scientific information system, which comprehensively integrates uPlc and ms with informatics and compliance tools.

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Waters Global Services – ready to help your team succeed. at waters, we don’t stop at systems. we bring you superior service, support, upgrades, and training through waters Global services. we can optimize your use of waters products with predictive system support with connections insiGht® remote services. waters ensures that your organization has the resources, knowledge, and support necessary to achieve more with uPlc technology.

ACQUITY UPLC H-Class System

nanoACQUITY UPLC System

PATROL UPLC Laboratory Analyzer System

PATROL UPLC Process Analyzer System

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Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 508 478 2000 F: 508 872 1990 www.waters.com

Waters, UPLC, ACQUITY UPLC, nanoACQUITY UPLC, PATROL UPLC, Xevo, UNIFI, Empower, MassLynx, and Connections INSIGHT are registered trademarks of Waters Corporation. The Science of What’s Possible, Auto•Blend, Auto•Blend Plus, High Definition Mass Spectrometry, and HDMS are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2013 Waters Corporation. Printed in the U.S.A. January 2013 720003267EN LB_AO-IGS

See how our customers are succeeding with ACQUITY UPLC:www.waters.com/voice

ACQUITY UPLC I-Class www.waters.com/iclass

ACQUITY UPLC H-Class www.waters.com/hclass

nanoACQUITY UPLC www.waters.com/nano

PATROL UPLC www.waters.com/patrol