fusion cell gene expression profile molecular mechanism of target gene regulation by dys::tgo...
Post on 20-Jan-2016
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![Page 1: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/1.jpg)
•Fusion cell gene expression profile
•Molecular mechanism of target gene regulation by Dys::Tgo
•Identify enhancer of dys targets: CG13196, CG15252 and mbo
•Identify dys fusion cell enhancer
dys-gal4::UAS-CD8-GFP btl-gal4::UAS-CD8-GFP
dys-gal4::UAS-CD8-GFP dys-gal4::UAS-CD8-GFP ; dys/dys
•Identify dys target gene
•Co-activator or repressor of Dys::Tgo?
![Page 2: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/2.jpg)
LP05454
Exon1 2 3 4 5 6 7 8 9 10
dys 2 (1.7kb)
dys1 (2.7kb)
dys un (3.8kb)
dys intron 2a (3.8kb)
dys intron2b (3.8kb)
Identify dys enhancer
dys1a (1.7kb)
dys1b (1.0kb)
dys intron1 (1.4kb)
dys1b-A (500bp) dys1b-B (500bp)
![Page 3: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/3.jpg)
Enhancer for brain, hindgut and anal pad
dys intron-1 dys intron-2b
dys intron-2a
![Page 4: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/4.jpg)
dys1 fragment contains dys fusion cell enhancer
dys un (S15-17)
dys1 (S12-17)
dys2 ( S15-17)
DT
DT
LT
DB
Leading edge?
DB
DT
LT
S14
S17S16
S14
![Page 5: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/5.jpg)
dys1b (S12-17)
S14
S16
dys1a (S14-17)
S15
S14
S12 S12
![Page 6: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/6.jpg)
dys target gene: CG15252
Exon1
CG15252 con (3.2kb)
9.0 kB 5’ upstream
CG15252 TCGTG (0.8 kb)
TCGTG TCGTG TCGTG
![Page 7: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/7.jpg)
CG15252 enhancer
CG15252 con
CG15252 TCGTGDT
S16
![Page 8: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/8.jpg)
CG15252 enhancer
TCGTG-a 185 bp
TCGTG-b 360 ssbp
TCGTG a+b (270bp) TCGTG c+d (100bp)
TCGTG-c 774 bp
TCGTG-d 792 bp
818bp
TCGTG to GATCCmutation: (waiting for result)
( NO?) (Yes!)
![Page 9: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/9.jpg)
CG15252 enhancer: 100bp with 2 TCGTG sites
DT
DT
S16
S17
![Page 10: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/10.jpg)
CG13196
500bp
350bp
100bp?
CG13196-500 CG13196-350
490bp
![Page 11: Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo](https://reader035.vdocument.in/reader035/viewer/2022070415/56649d415503460f94a1be82/html5/thumbnails/11.jpg)
Ungoing.....
CG15252 DT expression depends on Sal?
•CG15252-GFP expression in Sal mutant?•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-sal; UAS-dys•Compare CG15252 expression in btl-gal4; UAS-dys and btl-gal4, UAS-kni; UAS-dys
Co-activator or repressor of Dys::Tgo?
•Compare enhancer of CG13196 and CG15252, identify potential binding sites (drifter?)