FV10i-LIV FV10i-LIV Time-lapse Imaging Time-lapse Imaging

Strategy and Marketing Department

Acquisition conditionAcquisition condition

Location: Olympus Techno Lab Tokyo

Instrument : FV10i-LIV, Sample holder for three dishes （ FV10-HOD3 ）

Setting Setting

COCO22 　　 gas cylindergas cylinder 　　　　

　　

Gas blender Gas blender 　　 　　　　COCO22 ：： 6%6%

Air filterAir filter

TubeTube inner Dia 2mminner Dia 2mm

Glass bottle Glass bottle (Ultra Pure Water)

1 CO2 gas cylinder 　　

2 Gas blender

3 Glass bottle

4 Tube (inner Dia 2mm )

5 Tube joint 　

Tube joint

＊ In case the number of dishes is less than three, make sure to use dummy sample, i.e. empty dishes, not to have empty position.

HeLa cellsHeLa cells 　　 Actin-YFPActin-YFP 　　

Sample ： HeLa cells Dye ： YFP (Actin)Lens: 60XW NA1.2 Laser wavelengths: 473nmTime Interval: Every 3.5 minutes (TOTAL 12h)No Z projection movie

Sample ： HeLa cells Dye ： YFP (Actin)Lens: 60XW NA1.2 Laser wavelengths: 473nmTime Interval: Every 3.5 minutes 　　　　　　　　　　　　　 (TOTAL 12h)No Z projection movie

HeLa cellsHeLa cells 　　 Actin-YFPActin-YFP