Gel pictures of cloning lab

Section1 Gel1

3 kb

5 kb

1.6 kb

M 1 2Group1 Group2 Group3

M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)

Section1 Gel2M 1 2

Group1 Group2

M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)

5 Kb

3 Kb

1.6 Kb

Section1 Gel3

3 kb

5 kb

1.6 kb

M 1 2Group1 Group2 Group3

M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)

Section1 “control gel”M 1 2 3 4 5 6

M: DNA 1Kb ladder; 1: Purified cut vector (it did not work. But the band should be in the position of red bar); 2:Cut vector (not purified); 3:uncut vector (labeled as PBS); 4: Purified insert (CAT cassette); 5: Cut insert plasmid (not purified); 6: uncut insert plasmid (labeled as CAT)

5 kb3 kb

1.6 kb

Section2 Gel1M 1 2 Group1 Group2 Group3

M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)

5 Kb

3 Kb

1.6 Kb

Section2 Gel2M 1 2 Group1 Group2 Group3

M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)

5 Kb

3 Kb

1.6 Kb

Section2 Gel3M 1 2 Group1

M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)

5 Kb

3 Kb

1.6 Kb