gene-up® listeria spp general protocol - food samples · 2017-12-11 · gene-up® listeria spp...

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ALOA ® 24-48 h at 35 ± 1°C GENE-UP® Listeria spp General Protocol - Food Samples 1 Detection Reconstitution buffer Lysis 5 min 5 µL 5 µL Spin down 10 sec <1 h run - + 45 µL 2 Confirmation CONFIRMATION Enriched sample 20 µL 25 g in 225 ml LPT broth 22-26 h at 35-37°C Listeria spp range REF 414059 GENE-UP ® Listeria spp Kit 192 tests REF 414057 GENE-UP ® Lysis Kit 192 tests REF 410848 LPT broth 6 x 225 ml REF 410846 LPT broth 6 x 100 ml REF 410849 LPT broth 4 x 3 L REF 420984 Dehydrated LPT broth 500 g REF 420985 Dehydrated LPT broth 5 Kg REF AEB520079 AEB520080 ALOA ® agar 120 / 20 plates REF 42072 Fraser Broth 20 tubes REF 414956 Fraser Broth 100 tubes REF 10300 API ® Listeria 10 strips Listeria spp PCR Mix

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Page 1: GENE-UP® Listeria spp General Protocol - Food Samples · 2017-12-11 · GENE-UP® Listeria spp Environmental Samples 1 Detection Reconstitution buffer Lysis 5 min 5 µL Spin down

ALOA®

24-48 h at 35 ± 1°C

GENE-UP® Listeria spp General Protocol - Food Samples

1 Detection

Reconstitution

buffer

Lysis

5 min

5 µL5 µL

Spin down 10 sec

<1 h run

- +

45 µL

2 Confirmation

CONFIRMATION

Enriched sample

20 µL

25 g in 225 ml

LPT broth

22-26 h at 35-37°C

Listeria spp rangeREF 414059 GENE-UP® Listeria spp Kit 192 testsREF 414057 GENE-UP® Lysis Kit 192 testsREF 410848 LPT broth 6 x 225 mlREF 410846 LPT broth 6 x 100 mlREF 410849 LPT broth 4 x 3 LREF 420984 Dehydrated LPT broth 500 gREF 420985 Dehydrated LPT broth 5 KgREF AEB520079 AEB520080 ALOA® agar 120 / 20 platesREF 42072 Fraser Broth 20 tubesREF 414956 Fraser Broth 100 tubesREF 10300 API® Listeria 10 strips

Listeria spp

PCR Mix

bioMérieux S.A.

69280 Marcy l’Etoile, France

Tél. : 33 (0)4 78 87 20 00

Fax : 33 (0)4 78 87 20 90

www.biomerieux.comwww.biomerieux-industry.com

Page 2: GENE-UP® Listeria spp General Protocol - Food Samples · 2017-12-11 · GENE-UP® Listeria spp Environmental Samples 1 Detection Reconstitution buffer Lysis 5 min 5 µL Spin down

GENE-UP® Listeria spp Environmental Samples

1 Detection

Reconstitution

buffer

Lysis

5 min

5 µL5 µL

Spin down 10 sec

<1 h run

- +

45 µL

2 Confirmation

CONFIRMATION

18-24 h at 35-37°C

1 swab

in 10 ml

LPT broth

1 sponge

in 100 ml

LPT broth

20 µL

ALOA®

24-48 h at 35 ± 1°C

Enriched sample

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Listeria spp rangeREF 414059 GENE-UP® Listeria spp Kit 192 testsREF 414057 GENE-UP® Lysis Kit 192 testsREF 410848 LPT broth 6 x 225 mlREF 410846 LPT broth 6 x 100 mlREF 410845 LPT broth 20 x 10 mlREF 410849 LPT broth 4 x 3 LREF 420984 Dehydrated LPT broth 500 gREF 420985 Dehydrated LPT broth 5 KgREF AEB520079 AEB520080 ALOA® agar 120 / 20 platesREF 42072 Fraser Broth 20 tubesREF 414956 Fraser Broth 100 tubesREF 10300 API® Listeria 10 strips

Listeria spp

PCR Mix

bioMérieux S.A.

69280 Marcy l’Etoile, France

Tél. : 33 (0)4 78 87 20 00

Fax : 33 (0)4 78 87 20 90

www.biomerieux.comwww.biomerieux-industry.com

bioMérieux Industry

595 Anglum Road

Hazelwood, MO 63042 • U.S.A.

Tel: (800) 634 7656

Fax: (314) 731 8678

bioMérieux Canada, Inc.

7815 Henri-Bourassa Boulevard West

St. Laurent (Québec) • Canada H4S 1P7

Tel: (1) 514 336 7321

Fax: (1) 514 807 0015

www.biomerieux-usa.com