Genetic analysis

Susceptibillity genes

Samples collecting for identification of the presence of Papillomavirus infection

Patients with skin tumors (immuno-competent population, transplanted patients, Epidermodisplasia Verruciformis patients,

HIV patients)

Biopsy

Molecular and histologic analyses to determine the presence of Papillomavirus infection

SPECIALITY CLINICS FOR DIAGNOSIS AND TREATMENT OF HUMAN PAPILLOMAVIRUS (HPV)-RELATED CUTANEOUS

NEOPLASMS

SPECIFIC AIMSSPECIFIC AIMS

Hair bulbs

Blood

Genotipo di HPV

Controllo interno Controllo interno

Universale Universale

Genotipo di HPV

Controllo interno Controllo interno

Universale Universale

Cutaneous swab

Genotyping PCR Real Time IHCISH FISH

CHARACTERIZATION OF A STATISTICALLY SIGNIFICANT NUMBER OF PATIENTS IN ORDER TO ESTABLISH PROTOCOLS FOR PREVENTION,

DIAGNOSIS AND TREATMENT OF CUTANEOUS NEOPLASMS ASSOCIATED TO HPV INFECTION

CELL CULTURES DEVELOPMENTCELL CULTURES DEVELOPMENT

SieroMonolayer cultures

MOLECULAR, CELLULAR AND HISTOLOGIC ANALYSES AIMED TO:

IDENTIFY THE MOLECULAR MECHANISMS INVOLVED IN THE IDENTIFY THE MOLECULAR MECHANISMS INVOLVED IN THE CONTROL OF PAPILLOMAVIRUS REPLICATION AND IN THE CONTROL OF PAPILLOMAVIRUS REPLICATION AND IN THE PROCESS OF HPV-RELATED SKIN TUMORS DEVELOPMENTPROCESS OF HPV-RELATED SKIN TUMORS DEVELOPMENT

EVALUATE THE EFFECT OF PHARMACOLOGIC ACTIVE EVALUATE THE EFFECT OF PHARMACOLOGIC ACTIVE PRINCIPLES INTENDED TO HPV INFECTION WITH PRINCIPLES INTENDED TO HPV INFECTION WITH

ANTINEOPLASTIC PROPERTIESANTINEOPLASTIC PROPERTIES

IDENTIFY A “RISK PROFILE” TO SKIN TUMORS BASED ON IDENTIFY A “RISK PROFILE” TO SKIN TUMORS BASED ON THE VIRAL GENOTYPING ASSAYTHE VIRAL GENOTYPING ASSAY

COMPLEMENTATION OF CLINICAL SERVICES WITH COMPLEMENTATION OF CLINICAL SERVICES WITH RESEARCH AND DEVELOPMENT ACTIVITIESRESEARCH AND DEVELOPMENT ACTIVITIES

Development of experimental models in vitro using primary human keratinocytes from healthy donors and patients harbouring HPV

infection

ORGANOTYPIC CULTURES