genetic engineering in plants — transgenic plants 第十章 植物的基因工程
TRANSCRIPT
![Page 1: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/1.jpg)
Genetic Engineering in Plants—Transgenic Plants
第十章 植物的基因工程
![Page 2: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/2.jpg)
Agrobacterium
tumefaciens
and Crown galls
![Page 3: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/3.jpg)
Transformation of plant cells byAgrobacterium tumefacie
ns
Transformation of plant cells byAgrobacterium tumefacie
ns
![Page 4: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/4.jpg)
Agrobacteria cause crown gall tumors in plants
![Page 5: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/5.jpg)
Structure of the octopine and nopaline plasmids
Structure of the octopine and nopaline plasmids
![Page 6: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/6.jpg)
Structure of the nopaline Ti plasmid pTi C58LB---left borderRB---right border
![Page 7: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/7.jpg)
Map of the nopaline T-DNA region
Map of the nopaline T-DNA region
![Page 8: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/8.jpg)
Formation of a cointegrate Ti plasmid
Formation of a cointegrate Ti plasmid
![Page 9: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/9.jpg)
Binary vector system baced on Bin19
Binary vector system baced on Bin19
![Page 10: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/10.jpg)
A GUS vectorA GUS vectorCaMV-cauliflower mosaic virusGUS-glucuronidase NPTII---neomycin phospho- trasferase II
![Page 11: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/11.jpg)
Regeneration of transgenic plants by leaf
discs
Regeneration of transgenic plants by leaf
discs
![Page 12: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/12.jpg)
Regeneration of plantsfrom protoplastsAuxins/cytokinins↑,Root developmentAuxins/cytokinins↓,Shoot development
![Page 13: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/13.jpg)
Regeneration of leaf disks infectedby Agrobacterium
![Page 14: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/14.jpg)
Transfer of T-DNA from Agrobacterium in to aplant cell---Vir proteins andT-DNA transfer
![Page 15: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/15.jpg)
Transfering genes into plant cells by cointegration using T-DNANPTII---neomycin phospho-trasferaseII
![Page 16: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/16.jpg)
Transfer of an antisensegene for polygalacturonase(PG) into tomato cells by using a binary vectorCaMV-cauliflower mosaic virusGUS-glucuronidase
![Page 17: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/17.jpg)
Antisense technologyAntisense technology
![Page 18: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/18.jpg)
The antisense RNA procedure for blocking or reducing the level of expression of a specific gene
![Page 19: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/19.jpg)
The regulation of biosynthesis of Ethylene (ETH)
![Page 20: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/20.jpg)
反义基因在耐储藏番茄育种中的应用
ACC gene ( 1- 氨基丙环烷羧酸氧化酶, 乙烯合成途径酶类 )
anti-sense ACC-mRNA ACC-mRNA
乙烯合成被抑制
(-) 5 3 mRNA
(-) 5 3(+) 3 5 cDNA
anti-sense ACC gene pCaMV35s-
pCaMV35s-
![Page 21: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/21.jpg)
耐储藏番茄 华番一号
我国第一个基因工程农作物品种
![Page 22: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/22.jpg)
Systemic infection of a plantwith recombinant geminivirus VectorsGene A---Replication in plant cellsGene B---Infectivity
![Page 23: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/23.jpg)
Direct transfer of DNA intoplant cells by microprojectilebombardement (shotgun)
![Page 24: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/24.jpg)
Stable transformation of plastids in tobacco plantsSp---spectinomycinSm---streptomycin
![Page 25: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/25.jpg)
Cloning a plant gene bytransposon tagging
![Page 26: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/26.jpg)
Cloning plant genes by T-DNA insertionalMutagenesisEMS---ethylmethane sulfonate
![Page 27: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/27.jpg)
Protection of plants fromtobacco mosaic virus (TMV) infection by transgenic expression of the TMV coat protein(CP)
![Page 28: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/28.jpg)
Established Bt and Roundup ready crops
![Page 29: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/29.jpg)
Killing insect larvae with transgenic cotton plants that express the Bacillus thuringiensis(Bt) toxin gene
![Page 30: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/30.jpg)
苏云金芽胞杆菌( BT )毒素蛋白基因
![Page 31: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/31.jpg)
苏云金芽胞杆菌( BT )毒素蛋白基因
转 BT 基因水稻抗稻纵卷叶螟
抗病基因+抗虫基因+优质基因
![Page 32: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/32.jpg)
Engineering herbicide-resistance plants
![Page 33: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/33.jpg)
Roundup Ready® 转抗除草剂基因的
大豆和玉米
![Page 34: Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程](https://reader036.vdocument.in/reader036/viewer/2022081501/56649c805503460f949383d3/html5/thumbnails/34.jpg)
Plants as bioreactors to produce antibodies