genetic engineering/recombinant dna
DESCRIPTION
Genetic Engineering/Recombinant DNA. The direct manipulation of an organisms genome using biotechnology New DNA is inserted into a host genome Host organism transcribes and translates that DNA into a protein not normally manufactured by that organism - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/1.jpg)
Genetic Engineering/Recombinant DNA
The direct manipulation of an organisms genome using biotechnology
New DNA is inserted into a host genome Host organism transcribes and translates
that DNA into a protein not normally manufactured by that organism
Important/Interesting Examples: Insulin(and other biopharmaceutical)producing bacteria & animals, GloFish, GMO’s
![Page 2: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/2.jpg)
![Page 3: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/3.jpg)
Gene Therapy The use of DNA as a drug to treat disease by
delivering therapeutic DNA into a patients cells.
Good gene is inserted using a virus to replace a mutated gene.
Has been used to treat: Choroderemia, Immunodeficiency diseases, chronic lymphocytic leukemia, Beta-thalassemia, and color-blindness in dogs
Speculative Uses:• Designer children and curing genetic diseases in vitro• Gene Doping to improve athletic performance
![Page 5: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/5.jpg)
DNA Fingerprinting The isolation, imaging and matching of
DNA sequences Used to identify individuals by matching
their DNA profiles Parental Testing, Criminal Investigations,
Identification of suspects & victims, natural disasters, acts of terrorism.
Restriction enzymes are used to cut DNA into small pieces that are then separated on a GEL.
![Page 6: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/6.jpg)
![Page 7: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/7.jpg)
BioInformatics Using computers to store, organize,
and compare the genomes of all living organisms
Uses:• Sequence Analysis & Comparison• Computational Evolutionary Biology• Genetics of Disease: Breast cancer,
Alzheimer’s• Oncogenomics: Analysis of mutations in
cancer• Analysis of causes of epidemics
![Page 8: Genetic Engineering/Recombinant DNA](https://reader035.vdocument.in/reader035/viewer/2022062815/568168f4550346895ddffeb9/html5/thumbnails/8.jpg)
Cloning Production of genetically identical
populations of existing organisms, cells or tissues
Important Uses:• Cloning Stem Cells• Cloning whole organisms• Cloning of Extinct & Endangered Species
Some organisms that have been cloned:• Tadpoles, Carp, mice, sheep, pig, cattle, cat,
dog, wolf, camel, Pyrenean Ibex