getting the most out of the plant diagnostic clinic - new mexico...
TRANSCRIPT
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Getting the Most out of the gPlant Diagnostic Clinic
Professional Development TrainingM h 17 2011March 17, 2011
Natalie Goldberg, Ext. Plant PathologistJason French, Plant Diagnostician, g
New Mexico State University
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NMSU Plant Diagnostic Clinicg• http://plantclinic.nmsu.edu
Li k t NMSU Di t Di ti S t• Link to NMSU Distance Diagnostic System – On-line submission system for diagnostics
• Submission Forms• Submission Forms• Information on how to submit samples (do’s and don’ts)• Publications• PowerPoint Presentations• Links to useful websites• Other resources
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NMSU Plant Diagnostic Clinic
• Support lab for the National Plant Diagnostic Network
• Participate in National Plant Disease Surveys
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NMSU Plant Diagnostic Clinic• Support lab for the
National Plant DiagnosticNational Plant Diagnostic Network
• Participate in NationalParticipate in National Plant Disease Surveys
Services provided free of Services provided free of charge when samples are submitted through thesubmitted through the county extension offices
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NMSU – Plant Diagnostic Clinic1993 1999 2004 2010
Funding University(Salary)
University & USDA
University & NPDN
Univ., NPDN & grants(Salary) USDA NPDN & grants
Personnel Director DirectorStudents
Director ClinicianADS*
Director ClinicianADS*ADS ADS
Lab Space No Yes Yes Yes
I t t d N N Y YIntegrated Lab
No No Yes Yes
Total Samples 350 1,300 6,300 9,000(2 450 Di )(2,450 Disease)
*Additional Diagnostic Specialists – Entomology and Weed/Plant ID
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Plant Clinic ClienteleU i it• University:– County Agents– Researchers – Extension Specialists
• Regulatory Agency:– USDA– NMDA
• National Surveys• Commercial:• Commercial:
– Crop Producers– Crop Consultants
L d P f i l– Landscape Professionals (Ornamental and Turf )
• Homeowners
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How many of you have submitted aHow many of you have submitted a sample to the diagnostic clinic and received one of these responses?received one of these responses?
1 Th l b itt d i i ffi i t f1. The sample submitted is insufficient for diagnosis
2 No plant pathogenic microorganisms were2. No plant pathogenic microorganisms were found associated with the sample submitted
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How many of you have submitted aHow many of you have submitted a sample to the diagnostic clinic and received one of these responses?received one of these responses?
1 Th l b itt d i i ffi i t f1. The sample submitted is insufficient for diagnosis*
2 No plant pathogenic microorganisms were2. No plant pathogenic microorganisms were found associated with the sample submitted**
*Unsatisfactory response**Often means, sample insufficient
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Training Goals
• Improve sample submissions to reduce p punsatisfactory responses– Emphasize the key ingredients needed for
an accurate diagnosis• Increase knowledge and use of the
online submission system (NMSUonline submission system (NMSU DDS)
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Diagnosing Plant Disorders• The process of determining the cause of an
abnormality• Diagnosis is a team effort
– GrowerS b itti t– Submitting agent
– Diagnostic clinic• Conclusions are derived from critical evaluation ofConclusions are derived from critical evaluation of
the plant and its environment• Requires a blend of good observational skills, q g ,
science, and experience
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The Diagnostic Process• An accurate diagnosis depends on:
– Early detection of plant problem – routine examination y p pof the plant.
– Accurate sample informationE i i f d li i d/ h– Examination of good quality specimens and/or photos
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Why is early detection important y y pfor an accurate diagnosis?
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Early diagnosis is important y g pbecause….
Dead plants tell no tales dand
Plant diagnosticians are not plant coroners!
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Organisms Associated with Plants• Saprophytes – a non-pathogenic
microorganism residing on the plant and feeding g g p gon decaying organic matter
• Primary organism – the organism (pathogen) h i di l ibl f h dithat is directly responsible for the disease
• Secondary organisms – organisms that are taking advantage of weakened plant tissuetaking advantage of weakened plant tissue– Weak pathogens or saprophytes
The longer o ait to cond ct laborator• The longer you wait to conduct laboratory tests, the harder it gets to find the pathogen
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Why is accurate and complete yinformation important for an accurate diagnosis?accurate diagnosis?
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Accurate and complete information is important because….• The only information provided by the sample• The only information provided by the sample
are the symptoms that are evident on that samplep– Samples are usually a small piece of the plant– Samples show symptoms at only a moment in time
(no information on symptom progression or spread)– Samples provide no information on how much of the
plant or how many plants are affectedplant or how many plants are affected– Samples don’t tell when the problem began
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Symptoms are important…
• Visual evidence that there is a problem with the plantproblem with the plant
• Starting point for investigating the cause of the problemthe cause of the problem
• Help us understand what plant processes are affectedprocesses are affected
• But, symptoms alone are not sufficient for diagnosissufficient for diagnosis
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Why aren’t symptoms sufficientWhy aren t symptoms sufficient for diagnosis?
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Symptoms
• Often develop away from the site of infection orthe site of infection or pathogen activity
• Change over time• Vary with severity
(virulence) of the pathogenVary due to age of plant at• Vary due to age of plant at time of infection
• Vary due to environmental yconditions during and after infection
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Why aren’t symptoms sufficient for diagnosis?
• Symptoms are not specific to the causal agent!
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Symptoms Develop Because….
• The pathogen interferes with the host plants cellular functions
and/orand/or
• The pathogen produces enzymes, toxins, or growth regulators which cause adverse conditions in the host plant
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Plant Processes Affected by Pathogens
• Disruption of growth (impaired cell development, impaired hormone production)p p )
• Disruption of absorption or transport of water, minerals, and carbohydrates (impaired roots or
)vascular tissue)• Disruption in reproduction (impaired flowering or
seed development)seed development)• Disruption in storage (loss of stored carbohydrates)• Disruption of secondary metabolism (impaired• Disruption of secondary metabolism (impaired
pigment development)
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Disruption in Absorption and Transport• Impaired absorption of
water and minerals -(root dysfunction)
• Impaired transport of water and minerals -(xylem)
• Impaired translocation• Impaired translocation of carbohydrates -(phloem)(p )
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Disruption in Absorption or Transport• Types of Symptoms
– Wilting• Potential Causal Agents
– Infectious diseases
– Yellowing or other nutrient deficiency symptoms
– Soil insects and animals– Moisture stress (too much
and not enough)symptoms– Necrosis– Loss of vigor
( i )
g )– Nutrient deficiency– Chemical or salt injury
Mechanical injury(progressive)– Defoliation (over time)
– Mechanical injury– Soil problems– Temperature extremes
(hi h d l )(high and low)
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Bottom line…• Symptoms are complex
– Symptoms are non-specific to causal agents y p p g– Symptoms develop based on whatever plant process(es)
are affectedPathogens (and abiotic disorders) often affect more than– Pathogens (and abiotic disorders) often affect more than one plant process at a time leading to complex symptomology
– Plants may be affected by more than one causal agent (abiotic and biotic) at a time - adds to complexbiotic) at a time adds to complex symptomology
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Critical Information Needs• When the problem began• Specific description of symptoms (including symptom
development)– Keep in mind what we won’t see on the samples
• Indication of whether or not the symptoms are spreading• Indication of whether or not the symptoms are spreading (on plant or to other plants)
• Indication of distribution or pattern (field situations)• Cultural practices (particularly irrigation)• Environmental conditions (prior to and during symptom
d l t)development)• Chemical use history (including fertilizer)
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Accurate and Complete Information
• Helps to:Helps to:– Determine if the plant is suffering from an
infectious disease or an abiotic disorder– Develop a list of potential causal agents
• Helps to narrow down the diagnostic tests that p gwill be needed (saves time and money)
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Plant Specimen Submission Form• Found online
(http://plantclinic.nmsu.(http://plantclinic.nmsu.edu)
• Print and keep blank pforms in the office
• Helps to remind you of the information needed
• Use to help you fill in the information for online submission
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Why are good quality samplesWhy are good quality samples important for an accurate diagnosis?diagnosis?
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Good Quality Samples• Are in the early to middle stages of disease
– Dead plant tell no talesp• Are fresh and in good condition
– Send or deliver to the Plant Diagnostic Clinic as gquickly as possible after collection
• Are representative of the problem – range of symptoms and a range of severity
• Contain the margin of disease
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Good Quality Samples• Are in the early to middle stages of disease
– Dead plant tell no tales• Are fresh and in good condition
– Send or deliver to the Plant Diagnostic Clinic as quickly as possible after collectionpossible after collection
• Are representative of the problem – range of symptoms and a range of severitysymptoms and a range of severity
• Contain the margin of disease
If we get samples that meet these criteria, we will make an accurate diagnosis!
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Sample Collection• Representative samples
– Select material that shows theSelect material that shows the symptoms you are concerned aboutI l d ll i l– Include all symptomatic plant parts – this may include roots if necessaryy
Remember, symptoms may occur away from the site of infection (inspect the whole plant)the whole plant)
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Sample Collection
• Select several samples that show a range of p gsymptoms (in varying stages of infection)
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Sample Collectionp
• Whenever possible, the sample should containWhenever possible, the sample should contain the margin of disease
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Sample Submission (Photos or Digital Images)• Photos or digital images can
b t l f l fbe extremely useful for diagnosis
• Think about what you see that the diagnostician may not bethe diagnostician may not be able to see
• Good pictures include:– Wide angle view of field,Wide angle view of field,
landscape or site– Mid-range images of damaged
areas, whole plants, branches, leaves etcleaves, etc.
– Top, bottom and side views• Be sure photos are in focus.
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Remember: Diagnosis is a team effort. The diagnosis received is only g yas good as the sample submittedand the information providedand the information provided.
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N M i St t U i itNew Mexico State University Distance Diagnostics System g y
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NMSU Distance Diagnostics System plantclinic.nmsu.edu
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New Mexico State University Di Di i SDistance Diagnostics System
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New Mexico State University Di Di i SDistance Diagnostics System
After a successfully login the NMSU Distance Diagnostics System menuDistance Diagnostics System menu bar is displayed.
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New Mexico State University Di Di i SDistance Diagnostics System
The “Submit a Sample” section allows you to :1. Submit an insect for
identificationidentification 2. Submit a plant sample for
evaluation 3 Submit a plant for3. Submit a plant for
identification
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New Mexico State University Di Di i SDistance Diagnostics System
The “Find Samples ” section allows you to :1. Search for a sample by
sample number2 Search for all samples you2. Search for all samples you
have submitted3. Search for all sample you
have access to (all sampleshave access to (all samples from your county)
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New Mexico State University Di Di i SDistance Diagnostics System
The “Your Account” section allows you to :1. Update your contact p y
information2. Change your
passwordpassword
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New Mexico State University Di Di i SDistance Diagnostics System
The “Help and Support” section allows you to :• Report technical p
problems to the system administrator
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New Mexico State University Di Di i SDistance Diagnostics System
T b it l li k th t fTo submit a sample click on the type of sample you would like to submit.
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New Mexico State University Di Di i SDistance Diagnostics System
Select a contact form the drop down list or pselect “Enter A New Contact”
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New Mexico State University Di Di i SDistance Diagnostics System
If you chose to enter a new client:
After selecting a client you will be prompted to: 1. Select a client with out modifying any
information
y1. Fill out all appropriate contact
information 2. Click “Continue” when
2. Choose a different client 3. Modify information for this client
completed
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New Mexico State University Di Di i SDistance Diagnostics System
Submitting Sample information:1. Choose Sample Type
• Digital – Photos will be• Digital Photos will be uploaded but no physical sample will be sent
Di it l d Ph i l• Digital and Physical –Photos will be uploaded and a physical sample will be sent
• Physical – No photos physical sample only
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New Mexico State University Di Di i SDistance Diagnostics System
Required Fields for Sample q pSubmission: • Submission Type
– Is the sample confidential: S l t Y NSelect Yes or No
• Date Sample Collected:• Sample Location:
– County and statey– Click on “Choose State and
County”• Description of the Problem
Images if you selected “Digital” or• Images if you selected Digital” or “Digital and Physical” Submission types
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New Mexico State University Di Di i SDistance Diagnostics System
Uploading Images:p g g
Five images or less1. Select “Browse” and select each
imageimage. 2. After all images have been
selected click on “Submit Sample”
Six to Twenty images1. Check the Box “Yes, use batch
upload”2 Cli k “S b it S l ”2. Click “Submit Sample”3. Select “Browse” and select each
image4 After all images have been4. After all images have been
selected click on “Submit Sample”
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Tracking Samples
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Tracking Samples
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Final Report
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New Mexico State University Di Di i SDistance Diagnostics System
If you have any questions comments orquestions, comments or concerns please contact Jason French at 575Jason French at 575 –646 – 1965 or jfrench@nmsu [email protected]