gram staining dr. susanti 1

8/11/2019 Gram Staining DR. SUSANTI 1

1/29

Microbiology laboratoric test


2/29

Gram Stain

Material

solutions of crystal violet, Grams

iodine (2 g potassium iodide in300 ml distilled water plus 1 giodine crystals), 95% ethanoland/or

isopropanol-acetone mixture(3:1 v/v), and

safranin

Bismark brown stain (for color-blind students)

clean glass slides

inoculating loop

Bunsen burner

bibulous paper

microscope

lens paper and lens cleaner

immersion oil

Hyphomonas (Hyphomicrobium)neptunium (ATCC 15444) grownin marine broth (Difco)

slide warmer

staining rack

Bacto Gram Stain Reagents fromDifco for the three-step Gramstain


3/29

Gram Stain


4/29

Gram Stain


5/29

Gram Stain


6/29

Gram Stain


7/29

Gram Stain


8/29

Gram Stain


9/29


10/29

Gram Stain


11/29

Gram Stain


12/29

Gram Stain

Examination the preparation under microscope ( x100) for confirmation


13/29

Microscopic result for Gram Stain


14/29

Microbiology laboratoric test


15/29

KOH staining

Direct microscopic examination of the specimens canbe performed using potassium hydroxide preparations.

Skin debris is placed on a glass slide

A drop of a 10%-30% KOH solution with ink (3 ml of

KOH solution mixed with 1 cartridge of ink) added. After applying a cover-slip, the slide is placed in a moist

chamber for 1015 minutes to permit clearing of thekeratin

It is then viewed under a microscope.


16/29

Specimen collection for KOH staining

Desinfectant with alcohol 70 %


17/29


Scrape the scale from the edge of the scaling lesion

onto a microscopic slide


18/29


Place no more than one or two drops of 10% KOH


19/29


Covering with coverslip


20/29


gentle warming will accelerate the dissolution of celluler debris


21/29


Examination the preparation under microscope use lowillumination (x10 and x 40) for confirmation


22/29


P.versicolor

Desinfectant with alcohol 70 %


23/29


P.versicolor

Remove scale with tape


24/29


P.versiclor

Covering the object glass with tape


25/29

Specimen collection for KOH staining - TV

Place no more than one or two drops of 10% KOH


26/29

Specimen collection for KOH staining - TV

Examination the preparation under microscope use lowillumination (x10 and x 40) for confirmation


27/29

Microscopic result for KOH staining


28/29



29/29


gram staining dr. susanti 1

Documents