June 22-28, 2018Hyatt Regency Clearwater

Beach Resort & Spa CLEARWATER BEACH, FL

Registration will open in mid-JanuarySponsored by

American Foundation for Donation and Transplantation

American Foundation for Donation and TransplantationHistocompatibility Specialist Course Registration

Registration will open in mid-January.Please print or type.

Name: ___________________________________________________

Affiliation: ________________________________________________

Business Address: __________________________________________

_________________________________________________________

City, State, Zip: ____________________________________________

Phone: ___________________________________________________

Email: ___________________________________________________

Emergency Contact (Name & Phone): ________________________

_________________________________________________________

Check one: h Director h Laboratory Supervisorh New Laboratory Director h Technologisth Laboratory Manager h Other: _____________________

A check must accompany this form to ensure registration. Make checks payable to American Foundation for Donation and Transplantation. Credit cards are accepted.

Mail check & form to: AFDT, 8154 Forest Hill Avenue, Suite 3 Richmond, VA 23235-3255

Registration Fees (in US dollars)Before May 22 h $1,600.00After May 23 h $1,800.00Optional Review Session h $150.00(Mon., June 25, 12-2:30 pm)

Refunds will be made when requested in writing before April 30, 2018. A $100 fee will be charged on all refunds. There will be no refunds after May 15, 2018.

For information and registration, contact:Arlene M. Skinner, ManagerContinuing Education & Conference Planning8154 Forest Hill Avenue, Suite 3,Richmond, VA 23235-3255Tel: 804-323-9893; Fax: 804-323-1300; Email: skinner@amfdt.org

Note: Hotel and travel arrangements and their costs will be the responsibility of course participants. Registration will be Friday, June 22, 2018 and course will end on Thursday, June 28 at 12 noon. Breakfast, lunch and breaks will be provided each day except lunch on Monday, June 25, UNLESS you are participating in the optional review session. Box lunches will be provided for these participants. On Wednesday, June 27, dinner will be provided during a Farewell Gathering.

Reservations: Hyatt Regency Clearwater Beach Resort & Spa https://aws.passkey.com/go/HIST2018 Cutoff for group rate: June 1, 2018

Room rates: $199 single or double occupancy, plus 13% tax.

Highly recommended review:1. Reviews in Immunogenetics, Vol. 1 and Vol. 3, 1999, Munksgaard,

Copenhagen, Denmark.2. Handbook of Human Immunology. 1997 Leffell, Donnenberg and Rose,

CRC Press.3. Manual of Clinical Laboratory Immunology, Fifth Edition, 1997, eds:

Rose, deMacario, Fold, Lane and Nakamura, ASM Press, Section R: “Transplantation Immunology and Immunogenetics” and Zachary, AA and Steinberg, AG: Statistical Analysis and Applications of HLA Population Data, pp. 132-140.

4. The HLA Facts Book, 2000, Steven G.E. Marsh, Peter Parham and Linda D. Barber, Academic Press, San Diego, CA.

5. HIPAA (http://hhs.gov/news/press/2001/01privacy.html) pp. 1-50.

Recommended review:1. ASHI Laboratory Manual, 2001 4th Edition, Editors: Hahn, A., et al.,

(Pub. ASHI, 17000 Commerce Parkway, Suite C, Mount Laurel, NJ 08054).2. Flow Cytometry First Principles, 1992, Givan, Alice, (Pub. Wiley-Liss,

New York).3. Flow Cytometry Principles for Clinical Laboratory Practice: Quality

Assurance for Quantitative Immunophenotyping, 1995, Marilyn Owensand, Michael Loken (Pub.Wiley-Liss, New York).

4. “Flow Cytometric Crossmatching in Solid Organ Transplantation”, 1994, RA Bray, In Methods of Cell Biology: Flow Cytometry, Vol 41, Darzynkiewics, Z., Crissman, HZ, and Robinson, JP eds. Chapter 7, pp 103-119.

5. Bray R., Nickerson P., Kerman R., and Gebel H. Evolution of HLA Antibody Detection: Technology Emulating Biology. Immunologic Research 29:41-53, 2004.

6. Bray R.A. and Gebel H.M. Clinical Utility of Flow Cytometry in Allogeneic Transplantation. in; Flow Cytometry in Clinical Diagnosis, 4th Ed.; Carey J. McCoy J. and Keren D. eds. pgs. 275-294. ASCP Press, Chicago,IL. 2007

Reading for DNA & Stem Cell Lectures:1. Hurley, C. DNA methods for HLA typing. http://www.dodmarrow.org under

scientific services/educational material. 2008.2. Hurley, C. Acquisition and use of DNA-based HLA typing data in bone

marrow registries. Tissue Antigens 49:323, 1997.3. Holdsworth, R. et al. The HLA Dictionary 2008: a summary of HLA-A, -B,

-C, -DRB1/3/4/5, -DQB1 alleles and their association with serologically defined HLA-A, -B, -C, -DR and -DQ antigens. Tissue Antigens 73:95, 2009.

4. Marsh, S. et al. Nomenclature for factors of the HLA system, 2010. Tissue Antigens, in press.

5. Robinson, J. et al. IMGT/HLA and IMGT/MHC: sequence databases for the study of the major histocompatibility complex. Nucleic Acids Research 31:311, 2005.

6. ASHI Standards related to DNA-based HLA testing.7. Cutler, C. and J. Antin. An overview of hematopoietic stem cell transplanta-

tion. Clinics in Chest Medicine 26:517, 20058. Copelan, E. Hematopoietic stem-cell transplantation. New England Journal

Medicine 354:1813, 2006

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Rai

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• P

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His

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Spe

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tude

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com

preh

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cou

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s co

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g:•

Lec

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su

mm

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eren

ces

and

bibl

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aph

y •

Pro

cedu

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wit

h a

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ons

CU

RR

ICU

LU

M:

Cou

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pres

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l be

in

did

acti

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d

inte

ract

ive

disc

uss

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for

mat

. Th

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ocu

s is

on

h

isto

com

pati

bili

ty t

esti

ng:

• T

heo

ry a

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prin

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e •

App

lica

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•

Pro

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anag

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PP

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AN

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EQ

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EM

EN

TS:

Tec

hn

olog

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in

His

toco

mpa

tibi

lity

Tes

tin

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ith

a m

inim

um

of

3 ye

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wor

kin

g

expe

rien

ce a

nd/

or a

for

mal

cou

rse

in i

mm

un

olog

y or

gen

etic

s; o

r

a n

ew l

abor

ator

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rect

or; a

ctiv

e A

SHI

mem

bers

hip

(rec

omm

ende

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and

pre-

requ

isit

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adin

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mpl

eted

.

Thi

s co

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will

hav

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limit

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umbe

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reg

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ants

; ear

ly r

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ecom

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HIST

OCOM

PATI

BILIT

Y SPE

CIAL

IST C

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CULU

M 20

18

FRID

AY, JU

NE 22

Reg

istr

atio

n 5

:00

- 8:

00 p

m

SATU

RDAY

, JUNE

23B

asic

Con

cep

ts o

f

Tra

nsp

lan

tati

on I

mm

un

olog

y•

“Law

s” o

f T

ran

spla

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tion

• H

isto

ry &

Its

Im

pact

• A

dapt

ive

Imm

un

ity

& M

olec

ula

r B

asis

of

All

orec

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Inn

ate

Imm

un

ity

Inn

ate

Imm

un

ity

• P

atte

rn R

ecog

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Rec

epto

rs•

Infl

amm

atio

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Cyt

okin

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nd

Ch

emok

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• N

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d N

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un

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mu

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& T

CR

Gen

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& A

cces

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Eff

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Cel

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Pro

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Mol

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H

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Typ

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• D

NA

Str

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an

d P

repa

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Den

atu

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nn

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ng,

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ligo

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Pro

be

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Lab

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; DN

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enci

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rmat

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cces

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th

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/HL

A a

nd

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Res

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Don

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spla

nta

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w

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Tra

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tra

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lan

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on P

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Sel

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Pro

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SUND

AY, JU

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un

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& T

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: Si

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Pat

hw

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popt

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of H

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su

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gen

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Ass

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Hyp

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Con

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mu

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s an

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of

Gen

e (A

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quen

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(G

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(P

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aplo

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an

d L

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ests

of

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tan

ce•

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tin

gen

cy t

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tes

tin

g

and

rela

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stat

isti

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Log

isti

c R

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amil

y-ba

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asso

ciat

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tes

ts

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C G

enet

ics

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HC

Gen

e C

ompl

ex –

Loc

i, G

ene

Cla

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-MH

C G

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, C

lass

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Cla

ss I

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izat

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nte

nan

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equ

ilib

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Cyt

okin

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m

MOND

AY, JU

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Soli

d O

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Tra

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mu

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llog

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Rej

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lin

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App

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to

Tol

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ndu

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Imm

un

omod

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• P

ath

olog

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All

ogra

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Met

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s of

ass

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graf

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Cli

nic

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in

T

ran

spla

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• C

ause

s of

ESR

D, i

ndi

cati

ons

and

con

trai

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spla

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mu

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upp

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Pro

toco

ls:

stan

dard

an

d n

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sp

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Imm

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su

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KP

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e A

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noo

n a

nd

Eve

nin

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Op

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al T

uto

rial

for

D

iplo

mat

e E

xam

(12:

00 p

m –

2:3

0 pm

)

TUES

DAY,

JUNE

26N

ext

Gen

erat

ion

Seq

uen

cin

g

Bas

ic P

rin

cip

les

of F

low

C

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Bas

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once

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t T

ran

spla

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low

Spe

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t In

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C i

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low

Cyt

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ple

men

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den

t A

ssay

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C A

ssay

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r C

ross

mat

ch•

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rfer

ence

wit

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has

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mu

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ssu

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blem

s an

d P

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efin

ing

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acce

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of

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nal

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tibo

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WED

NESD

AY, JU

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s: M

ech

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m o

f A

ctio

n

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ools

• A

nti

body

an

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ing

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All

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Em

ergi

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Tec

hn

olog

ies

in t

he

HL

A L

abor

ator

y –

Spon

sor

Pre

sen

tati

ons

THUR

SDAY

, JUNE

28Im

ple

men

tin

g an

Eff

ecti

ve Q

A

Pro

gram

• D

esig

nin

g a

QA

Pro

gram

• Q

ual

ity

Indi

cato

rs a

nd

Mon

itor

ing

• R

oot

Cau

se A

nal

ysis

• Q

AP

I•

Com

pete

ncy

Ass

essm

ent

Lab

orat

ory

Acc

red

itat

ion

• In

spec

tion

Pro

cess

– A

SHI

an

d C

AP

• O

ther

Reg

ula

tory

Age

nci

es-

CM

S, J

C, U

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