imaging fascia
DESCRIPTION
Imaging Fascia. Renee Stenbjorn, MPA, LMT. My goal:. Utilize images to inform the student of the complex structural system of fascia. Your goal?. Historical Definition. Fascia: general description, a band of tissue. - PowerPoint PPT PresentationTRANSCRIPT
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Imaging FasciaRenee Stenbjorn, MPA, LMT
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My goal:
Utilize images to inform the student of the complex structural system of fascia.
Your goal?
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Historical Definition
Fascia: general description, a band of tissue.
General meaning: undifferentiated tissue surrounding a more specialized tissue.
LANGEVIN, MD, H., HUIJING, PHD, P.. Communicating About Fascia: History, Pitfalls, and Recommendations.International Journal of Therapeutic Massage & Bodywork: Research, Education, & Practice, North America, 2, oct. 2009. Available at: <http://www.ijtmb.org/index.php/ijtmb/article/view/63>. Date accessed: 14 Jan. 2013.
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Current understanding
Forms of connective tissue:
Loose & Dense
Superficial & Deep
Multiple- & Single-layered
LANGEVIN, MD, H., HUIJING, PHD, P.. Communicating About Fascia: History, Pitfalls, and Recommendations.International Journal of Therapeutic Massage & Bodywork: Research, Education, & Practice, North America, 2, oct. 2009. Available at: <http://www.ijtmb.org/index.php/ijtmb/article/view/63>. Date accessed: 14 Jan. 2013.
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Gross Anatomy
Photography of anatomical structures:
What is it?
What do we learn?
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"Old-School" Histology
Gradations of pink, some purples, blues:
Procedures: Create a specimen
Mount on slides
Put drops of solutions on slide for specific period of time.
Rinse & mount.
Put in a slide box.
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Blood smear, Biology 101
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"Old-School" HistologyPut in a slide box.
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What do we learn from histology?
Highlights & differentiates:
Cells, fibers & tissues
Gives insight into cellular structures.
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Histology of Connective Tissue types: Dense Irregular
Abundant Collagen fibers, few cells.What type of tissues?
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Histology of Connective Tissue types: Dense Regular
Abundant Collagen fibers, arranged in same direction, f few cells.What type of tissues?
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Histology of Connective Tissue types: Areolar
Sparsely arranged fibers, not ordered in a predominant direction. Oftern refferd to as "Loose" but Dr. Langevin suggests this label is too mechanical in description
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Connective Tissue types: Bone
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Connective Tissue types: Blood
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Specialized Fascial Structures:
Superficial Fascia: aka: hypoderm, hypodermis, stratum subcutaneous, etc.
Continuous with the dermis, directly underneath the skin
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Specialized Fascial Structures:
Deep Fascia
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Specialized Fascial Structures:
Muscular Fascial layers:
Endo
Peri
Epi
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Specialized Fascial Structures:
Intermuscular Septa
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Specialized Fascial Structures:
Neurovascular Tract
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Specialized Fascial Structures:
Periosteum
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How did histology help us differentiate:
Regular vs. Irregular Fiber Arrangements
Layers of fascia
Structural differences
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Really old school...what Golgi saw
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VERY toxic...
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Imaging Cells & Fibers
Immunohistochemistry: We can localize molecular structures rather than just cellular structures.
Can measure DNA & RNA activity
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What is different?
Process of IHC
Fluorescent labeling
Co-localization of molecules
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A Study of Collagen in the Nervous System
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What is a collagen?
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How do we know where the collagen is...?
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Dissecting a human brain...
Dr. Liechnetz, MCV
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Compared to a mouse brain...
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Understanding Blots
What do those dark spots mean?
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Putting the theory to the test
Can we find Collagen XVIII (18) in the brain?
Using immunohistochemistry to locate structures.
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Example: Cerebellum staining
Collagen 18 found in Purkinje cells in the cerebellum!
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Let's take a look: layers of fascia
How images help us understand layers of tissue
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Confocal imaging
A method of converting IHC images into 3-D images that further our understanding of tissues.
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3-D images
Z Stack
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What have we learned from these images?
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The Neuromuscular Junction
Understanding the relationship between nerve & muscle
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The Extracellular Matrix
What's in it?
Why do we care?
Key to hydration.
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Fascial Innervation
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Sensory Mechanism
Tensigrity architecture allows cells to sense mechanical changes.
These changes result in control of cell function & tissue development
Donald Ingber
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Connective tissue: a body-wide signaling network?
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Looking even deeper with Electron Microscopy
Seeing the textures
Understanding the structures
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The myofascia...
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Imaging with Ultrasound
From treatment to imaging: Principles in imaging with US.
What can we see with US: what do these images tell us?
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Ultrasound for differentiating layers
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Imaging trigger points!!
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Video Imaging
It's a living tissue.
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What does all of this tell us about treatment??