in the city of chicago, it is illegal to speak english
TRANSCRIPT
Pennsylvania
• When driving, the man must run in front of the car when the woman is driving, firing a rocket every mile, phoning the chief of police when they enter a town and disassembling the car and hiding it under a bush should they pass a horse
Neo-Darwinism
• Nature selects not only for certain phenotypes, but for the alleles that produce those phenotypes
• Populations whose allele frequencies change over time are said to be evolving.
Gene Pool & Allele Frequency
• Gene Pool = all of the different alleles in a naturally reproducing population
• Allele Frequency: the % of a given allele form for a trait present in the gene pool
Natural Selection & Alleles
• Since “selected” individuals have higher differential reproduction, their alleles become more frequent in the gene pool
• As a population shifts in terms of allelic frequencies, the phenotypic frequency may also change
Hardy-Weinberg Equilibrium
• If the allele frequencies of a gene pool do not change over time, the gene pool does not undergo evolution.
• KEY POINT: Individuals DO NOT EVOLVE; populations DO
Requirements of Populations At Hardy-Weinberg Equilibrium
• No selective pressures (all must have equal chance of surviving)
• No differential reproduction
• No mutations
• No immigration or emigration
• No changes in allele frequencies
Back In The Real World…
• Populations rarely ever exhibit many of the characteristics of HW Equilibrium
• Mutations and environmental change are random
• Alleles that code for adaptations may be reduced to variations in a new environment
Genetics of Sickle Cell Anemia
• Sickled red blood corpuscles caused by incompletely dominant allele (s).
• Individuals with ss genotype have terminal sickle cell anemia
• Individuals with As genotype have sickle cell anemia but do not die from the disease. In addition, they will not contract Malaria
• Individuals with AA genotype do not have sickle cell anemia, but will die from Malaria if infected
Setup
• Obtain five (5) containers and label1. Population2. AA non-sickle cell & non-Malarial3. As sickle cell4. ss sickle cell, terminal5. Non-surviving Alleles
Place 150 white/red beans and 50 black beans in the first container. These beads represent the following alleles-White or Red = A Black = s
Natural Selection
• Have one partner randomly remove 2 beans at a time. These two alleles represent an individual and their phenotype/genotype.
• It will take 100 turns to apply the selective pressure to each individual in the pop.
• For groups 4-6, apply the selective pressure of Malaria of 40% (i.e. 40 of 100 turns)
Applying The Selective Pressures…• If two white/red beans (AA) are selected
during a non-Malarial season, place both beads in the first cup, representing surviving alleles
• If two white/red beads (AA) are selected during a Malarial season (Africa ONLY), place both beans in the Non-Surviving Container
• If two black beans (ss) are selected at any time, place them in the fourth (“ss”) cup.
• If a white/red and a black are simultaneously selected, place both beads in the 3rd cup (As/sickle cell)
Totaling The Survivors
• After 100 pairs of alleles have been selected, kill off the “ss” terminal sickle cell alleles by pouring cup #4 into cup #5
• Tally the number of white/red beans (A allele) and the number of black beans (s allele) from cups #2 & 3 combined
• Using the formulas in the lab (under data table 1), calculate the allele frequencies (i.e. how many A or a alleles/total number of alleles in survivors) for the A and s alleles
Successive Generations• Take the surviving A and s alleles from cups 2 & 3 and
recombine them in cup #1• Repeat the selection process, pulling out pairs of
alleles/beans and applying the Malarial selective pressure at the same 40% infection rate (AFRICA ONLY).
• As in the first trial, discard the alleles for the non-surviving and ss individuals (cups #4&5) and tally the combined # of white/red (A) and black (s) beads.
• Calculate the new allele frequencies using the formulae under data table #2
• ADD SPACE IN YOUR DATA TABLE FOR A THIRD and FOURTH TRIAL. Repeat this process through four generations
CALCULATING & SHARING
• Calculate the allele frequencies for the dominant (A) allele and the recessive (a) allele for each generation.
• Share your data with the other side of the room.
• Answer the analysis questions, citing specific detail.