innova plex 2008 winter catalog

17
INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com Taq DNA Polymerase Descriptions Innova Plex Taq DNA Polymerase is suitable for routine PCR. It is a thermostable enzyme that can withstand prolonged incubation at temperature up to 95’C without significant loss of activity. It is a recombinant type purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E.coli. This enzyme is highly purified for its maximum activity and free of bacterial DNA through a unique purification system. For comprehensive optimization of PCR conditions 5X Band Finder TM solution is also included. Benefits High quality, High purity, and low price Easy and simple PCR optimization with Band Finder TM can help easy optimization to amplify various kinds of target DNA. Band Finder TM can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA. The included reaction buffer is specially designed for high-yield PCR production. A high quality of dNTP mixture is included for best result of your works Quality High Activity Applications High Purity To examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purifi- cation, template free PCR was performed by using Taq DNA polymerase gene primer. for Routine PCR M 1 2 3 4 5 6 7 Innova Plex Competitor Innova Plex Competitor 30 cycles 35 cycles M: 1kb Plus Ladder(Invitrogen) 1. 5 units 2. 2.5 units 3. 1.25 units 4. 0.625 units 5. 0.313 units 6. 0.156 units 7. 0.078 units M: 1kb Plus Ladder(Invitrogen) 1.2.5 U 2.1.25 U 3.0.625 U 4.0.313 U M: 1kb Plus Ladder(Invitrogen) 1.2.5 U 2.1.25 U 3.0.625 U 4.0.313 U M: 1kb Plus Ladder(Invitrogen) 1. Innova Plex 2. Competitor T 3. Competitor R 4. Competitor S 1. PCR amplification with Innova Plex Taq - Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplification cycle: 35cycles - Reaction volume:50ul 2. Activity of Taq DNA Polymerase Using original enqyme & buffer - Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplification cycle: 30cycles - Reaction volume:50ul 3. Activity of Reaction Buffer Using original enzyme & Innova Plex buffer - Template: Human Genomic DNA(100ng) - Target size: 1.9kb - Amplification cycle: 30cycles - Reaction volume:50ul Test for Plasmid DNA Contamination - Target gene: Taq DNA Polymerase - Target size: 0.7kb - Amplification cycle: 30 or 35 cycles - Reaction volume:50ul 1

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Page 1: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Taq DNA Polymerase

DescriptionsInnova Plex Taq DNA Polymerase is suitable for routine PCR. It is a thermostable enzyme that can withstand prolonged incubation at temperature up to 95’C without signifi cant loss of activity. It is a recombinant type purifi ed from the cloned Thermus aquaticus DNA polymerase gene expressed in E.coli. This enzyme is highly purifi ed for its maximum activity and free of bacterial DNA through a unique purifi cation system. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.

Benefi tsHigh quality, High purity, and low priceEasy and simple PCR optimization with Band FinderTM can help easy optimization to amplify various kinds of target DNA. Band FinderTM can resolve the PCR problems of high GC contents or complicated structures of primers and target DNA.The included reaction buff er is specially designed for high-yield PCR production.A high quality of dNTP mixture is included for best result of your works

QualityHigh Activity

ApplicationsHigh PurityTo examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purifi -cation, template free PCR was performed by using Taq DNA polymerase gene primer.

for Routine PCR

M 1 2 3 4 5 6 7

Innova Plex Competitor

Innova Plex Competitor

30 cycles 35 cycles

M: 1kb Plus Ladder(Invitrogen)1. 5 units2. 2.5 units3. 1.25 units4. 0.625 units5. 0.313 units6. 0.156 units7. 0.078 units

M: 1kb Plus Ladder(Invitrogen)

1.2.5 U

2.1.25 U

3.0.625 U

4.0.313 U

M: 1kb Plus Ladder(Invitrogen)

1.2.5 U

2.1.25 U

3.0.625 U

4.0.313 U

M: 1kb Plus Ladder(Invitrogen)

1. Innova Plex

2. Competitor T

3. Competitor R

4. Competitor S

1. PCR amplifi cation with Innova Plex Taq- Template: Human Genomic DNA(100ng)- Target size: 1.9kb- Amplifi cation cycle: 35cycles- Reaction volume:50ul

2. Activity of Taq DNA PolymeraseUsing original enqyme & buff er- Template: Human Genomic DNA(100ng)- Target size: 1.9kb- Amplifi cation cycle: 30cycles- Reaction volume:50ul

3. Activity of Reaction Buff erUsing original enzyme & Innova Plex buff er- Template: Human Genomic DNA(100ng)- Target size: 1.9kb- Amplifi cation cycle: 30cycles- Reaction volume:50ul

Test for Plasmid DNA Contamination- Target gene: Taq DNA Polymerase - Target size: 0.7kb- Amplifi cation cycle: 30 or 35 cycles- Reaction volume:50ul

1

Page 2: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

ProductsCatalog No. Product Name Units

2

Taq DNA Polymerasefor Routine PCR

ITD16-R500 Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) 500U ITD16-R25h Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml) (500*5/pkg) 2500U ITD16-R50h Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 4.0ml) (500*10/pkg) 5000U

Page 3: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

fx-Taq DNA Polymerase for High Specifi city, High Productivity

DescriptionsInnova Plex fx-Taq Polymerase is a modifi ed enzyme of Innova Plex Taq polymerase and suitable for routine PCR, MS Genotyping, SNP, and Multiplex-PCR etc. The modifi cation enhances the amplifi cation specifi city and minimizes the dimmer formation and non-specifi c PCR products. Unlike other chemically modifi ed PCR enzymes, fx-Taq polymerase shows high productivity as well as the high specifi city. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly purifi ed for its maximum activity and free of bacterial DNA through a unique purifi cation system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder™ solution is also included.

Benefi tsHigh quality, hgh purify, and low price.Easy and simple PCR optimization with Band Finder™ can help easy optimization to amplify various kinds of target DNA. Band Finder™ can resolve the PCR problems of high GC contents or complicated struc-tures of primers and target DNA. Clean results by minimized primer dimer and non-specifi c band.The included reaction buff er is specially designed for high-yield PCR production.A high quality of dNTP mixture is included for best result of your works.

Contentsfx-Taq DNA Polymerase 5U/ul10x fx-Taq reaction buff er 10mM dNTP Mix (each 10mM) 5x Band Finder

Storage Buff er50% Glycerol 20mM Tris-HCl (pH8.0) 100mM KCl 0.1mM EDTA 1mM DTT 0.5% Tween 20 0.5% Nonidet P-40

ProductsCatalog No. Product Name Units

IFT06-R500 fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4ml) 500UIFT06-R25h fx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 2.0ml) (500*5/pkg) 2500U

Page 4: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

hx-Taq DNA Polymerase for High Specifi city PCR

DescriptionsInnova Plex hx-Taq polymerase is a modifi ed enzyme of Innova Plex Taq polymerase and suitable to use for hot start PCR, MS genotyping, SNP, Multiplex-PCR, and real-time PCR etc. The chemical modifi cation makes the enzyme inactive at low temperature and dramatically reduces the non-specifi c amplifi cations during the PCR. This enhances the amplifi cation specifi city and minimizes the dimmer formation and non-specifi c PCR products. With this innovative enzyme the problematic PCRs due to high GC or structural problems of target DNA or primers could be easily solved. This enzyme is highly purifi ed for its maximum activity and free of bacterial DNA through a unique purifi cation system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band Finder™ solution is also included.

Benefi tsHigh quality, hgh purify, and low price.Easy and simple PCR optimization with Band Finder™ can help easy optimization to amplify various kinds of target DNA. Band Finder™ can resolve the PCR problems of high GC contents or complicated struc-tures of primers and target DNA. The included reaction buff er is specially designed for high-yield PCR production.A high quality of dNTP mixture is included for best result of your works.

Contentshx-Taq DNA Polymerase 2.5U/ul10x hx-Taq reaction buff er 10mM dNTP Mix (each 10mM) 5x Band Finder™

Storage Buff er50% Glycerol 20mM Tris-HCl (pH8.0) 100mM KCl 0.1mM EDTA 1mM DTT 0.5% Tween 20 0.5% Nonidet P-40

ProductsCatalog No. Product Name Units

IHT06-R250 hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.2ml) 250UIHT06-R10h hx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.8ml) (250U*4/pkg) 1000U

Page 5: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

EFx-Taq DNA Polymerase for Long-range PCR

ProductsCatalog No. Product Name Units

IEF16-R250 EFx-Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.3ml) 250U

M1: 1kb Plus Ladder(Invitrogen)M2: Lambda/Hind III

1. 14 kb2. 17kb3. 20kb4. 22kb

M 1 2 3 4 5 6

DescriptionsInnova Plex EFx-Taq DNA Polymeraseis ideal for long(up to 40kb) and accurate PCR. It has higher fi delity, productivity and processing ability than Taq polymerase. It is a kind of blend product, a mixture of Taq and pfu DNA polymerase. Several special cofactors added to the enzyme mixture enhance the productivity of long PCR. This enzyme is highly purifi ed for its maximum activity and free of bacterial DNA through a unique purifi cation system. For easy and stable PCR reactions high-quality dNTP mixture is included. For comprehensive optimization of PCR conditions 5X Band FinderTM solution is also included.

Benefi tsHigh quality, hgh purify, and low price.Easy and simple PCR optimization with Band Finder™ can help easy optimization to amplify various kinds of target DNA. Band Finder™ can resolve the PCR problems of high GC contents or complicated struc-tures of primers and target DNA. The included reaction buff er is specially designed for high-yield PCR production.A high quality of dNTP mixture is included for best result of your works. (The quality of dNTP mixture is one of the most critical things for long-PCR)

QualityHigh Activity1. PCR amplifi cation with EFx-taq DNA Polymerase

- Template: Human Genomin DNA - Target size: 14, 17, 20, 22kb - Amplifi caiton cycle: 35cycles - Reaciton volume: 50ul

2. Activity of EFx-taq DNA Polymerase

- Template: Human Genomin DNA - Target size: 17kb - Amplifi caiton cycle: 30cycles - Reaciton volume: 50ul

High PurityTo examine contamination of E.coli genomic and plasmid DNA in the process of Taq DNA polymerase purifi -cation, template free PCR was performed by using Taq DNA polymerase gene primer.

M1: 1kb Plus Ladder(Invitrogen)

1.EFx-Taq(2.5U)2.EFx-Taq(1.25U)3.Competitor A(Product A:2.5U)4.Competitor A(Product A:1.25U)5.Competitor A(Product A:2.5U)6.Competitor A(Product A:1.25U)

M 1 1 2 3 4 M 2

Page 6: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

pfu-X Taq DNA Polymerase for High Processivity & Fidelity PCR

ProductsCatalog No. Product Name Units

IPX 16-R250 pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.3ml) 250 U IPX 16-R500 pfu-X DNA Polymerase with 10mM dNTP MIX(each 10mM, 0.6ml) 500 U

DescriptionsInnova Plex pfu-X DNA polymerase is suitable for high-speed, high fi delity and long size PCR. It is applicable to high-fi delity PCR, such as the cloning experiments for protein expressions. The PCR product amplifi de by this enzyme have blunt-ends. Innova Plex pfu-X DNA polymerase has much higher activity and processing ability more than other brands of high-fi delity thermosatable enzymes.

Benefi tsHigh quality, high fi delity, high processivity, long range detection and short run time and low price.Because of blunt-ended PCR product, TA cloning is not available. For TA cloning of PCR product ampli-fi ed by this enzyme, PCR product is required to extend by Taq DNA polymerase.Easy and simply PCR optimization with Band Finder™ can help easy optimization to amplify various kinds of target DNA. Band Finder™ can resolve the PCR problems of high G+C contents or complicated structures of primers and target DNA.The included reaction buff er is specially designed for high-yield PCR production.

Contentspfu-X DNA Polymerase 2.5U/ul10x pfu-X Buff er10mM dNTP Mix (each 10mM) 5X Band Finder™

Storage Buff er50% Glycerol 50mM Tris-HCl (pH8.0) 100mM KCl 0.1mM EDTA 1mM DTT 0.1% Tween 20 0.1% Nonidet P-401mM PMSF

Page 7: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Plasmid Mini-prep Kit

ProductsCatalog No. Product Name Units

IPM01-C100 Plasmid Mini-prep Kit 100prepsIPM01-C200 Plasmid Mini-prep Kit 200preps

for rapid isolation of high-quality plasmid

DescriptionsInnova Plex Mini-prep kit is designed of rapid isolation of high-quality & high-quantity plasmid. The prin-ciple of plasmid purifi cation of this kit is based on the alkaline-lysis method and the binding properties of DNA on the silica-gel membrane. The high-quality plasmids isolated from this kit is adequate for routine molecular biology application such as automated sequencing, cloning , PCR, in vitro transcription, transfec-tion experiment etc. is also specially designed for high yield isolation of low-copy plasmid and much large size of circular DNA such as Cosmid or BAC clones.

Spin column typeBinding capacity: 20ug DNA/columnRecovery yield: above 80%

QualityComparative quality test Automatic sequencing result

Supplier A Innova Plex Supplier B

High-copynumberplasmid

High-copynumberplasmid

Low-copynumberplasmid

Low-copynumberplasmid

Large sizeofplamid

Large sizeofplamid

160

140

120

100

80

60

40

20

0Ban

d d

ensl

ty (r

elat

ive

valu

e)

Innova Plex Supplier A Supplier B

Page 8: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

PCR Purifi cation Kitfor DNA clean up from PCR and other enzymatic reactions

O B A O B A

M O Supplier A Innova Plex

M: size maker O: original sample(before purifi cation)

M O Supplier A Innova Plex Direct sequencing of a PCR product purifi ed using Innova Plex kit

90bp

300bp

1.5kb

4.0kb

14kb

Supplier A Innova Plex

Reco

very

Yie

ld

1.2

1.0

0.8

0.6

0.4

0.2

0.0Innova Plex Supplier A

Condition A: Innova Plex HY condition.2 volumes of isopropanol and 2 volumes of each binding buff er were applied to 1 volume of original PCR mixture.Condition B: Innova Plex DR conditions.5 volumes of binding buff ers were applied to 1 volume of original PCR mixture.

O. Original sample B. Condition B A. Condition A

Condition A B A B A B A B A BDNA size 90bp 300bp 1.5kb 4.0kb 14kb

ProductsCatalog No. Product Name Units

IPP02-C100 PCR Purifi cation Kit 100prepsIPP02-C200 PCR Purifi cation Kit 200preps

DescriptionsInnova Plex PCR purifi cation kit is designed for rapid and high-yield cleanup DNA from PCR and other en-zymatic reaction mixture. The high purity DNA isolated using this kit is adequate for any molecular biology applications. Especially, the introduced unique system (DR condition) for the effi cient removal of primer dimers, by-products of PCR, insures excellent results of automated sequencing and cloning.

Spin column typeDNA size: 70bp~20kbRecovery yield: above 80%Effi cient removal of primer dimer

Benefi tsOne-step DNA clean-up from PCR or other enzymatic reactionsApplicable to broad range of size(from 70 bp to 14kb)Two conditions are available for best results. Under the DR(dimer removal) condition, small fragment (less than 100bp) of DNA could be completely removed and you can obtain best results of automatic se-quencing or cloning works. Under the HY(high yield) condition, all of the applicable size of DNA (from 70bp to 14kb)could be obtained with above 80% yield.High reproducibility and stability

QualityComparative quality test

Clear primer dimers

Page 9: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Gel Extraction Kit

DescriptionsGel Extraction kit is designed for high-yield &high-quality isolation of any type of DNA(linear or circular size of 50 bp-20kb)from an agarose gel after gel electrophoresis. This kit is adequate for preparation of high quality DNA for automated sequencing , cloning, fl uorescent or radioactive labelling, in vitro transcription and other molecular biology applications.

Spin column type DNA size:50bp~20kbrecovery yield:above 80%(70bp~10kb) [Fragments of shorer than 700 bp or longer than 10kb show 50~70% yield]

Benefi tsEasy and safe purifi cation of DNA from agarose gelsPreparation of highly purifi ed DNA with highest yieldApplicable to broad range of size( from 70 bp to 14kb)High reproducibility and stability

QualityComparative quality test

ProductsCatalog No. Product Name Units

IGE03-C100 Gel Extraction Kit 100prepsIGE03-C200 Gel Extraction Kit 200preps

for high yield DNA isolation from agarose gel

Automatic sequencing result

Supplier A Innova Plex Supplier B

90bp 200bp 300bp 500bp 700bp 1.2kb 1.5kb 4.0kb 14.0kb

90bp

200bp

300bp

500bp

700bp

1.2kb

1.5kb

4.0kb

14.0kb

Reco

very

Yie

ld

1.2

1.0

0.8

0.6

0.4

0.2

0.0

Innova Plex Supplier A

Size of eluted DNA

Page 10: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Gel & PCR Purifi cation Kit

DescriptionsInnova Plex Gel & PCR purifi cation System Kit is designed to extract fragments of 50bp~20kb from an agar-ose gel after gel electrophoresis or to purify PCR products directly from a PCR reaction mixtures. The introduced unique system (DR condition) is highly effi cient to remove the primer dimers, by-products of PCR . This kit can be used for preparation of high quality DNA for automated sequencing, cloning, fl uo-rescent or radioactive labeling, in vitro transcription and other molecular biology applications.

Spin column type DNA Size : 50bp-20kbRecovery Yield : above 80%(70bp-10kb)

Benefi tsApplicable to gel extraction and PCR purifi cation by one kitHigh yield and high purity DNA purifi cationHigh quality purifi cation of DNA from 70bp to over 14kb

CompostionUB : Universal buff erWB : Washing buff er(80% EtOH)Only empty bottle is provided because Ethanol is easy to volatilize or deteriorate.EB : Elution buff erSpin column

QualityPCR Purifi cation

ProductsCatalog No. Product Name Units

IGP04-C100 Gel & PCR Extraction System 100prepsIGP04-C200 Gel & PCR Extraction System 200preps

for DNA clean up from PCR and other enzymatic reactions

Gel Extraction

M C A - 1 B - 1 A - 2 B - 2 M M C Q SG SU M C Q SG SM

glo-F-R1

glo-F-R3

PCR Purifi cation M: 1kb’ DNA LadderA-1: PCRB Dimer RemovalB-1: Gel & PCR Dimer RemovalA-2: PCRB HighYieldB-2: Gel & PCR High YieldC: Control(original sample)

Gel Extraction M: 1kb’ DNA LadderC: Control(original sample)Q: Q company sampleSG : GEB prepSU: Gel & PCR prep

glo-F-R5

M C Q SG SU M C Q SG SM

Page 11: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Template: Plasmid

Fig 1. Sequence Save Solution Eff ect of DNA Sequencing

Template: PCR product

Big dye 0.125 ulBig dye 0.25 ulBig dye 1.0 ul

Big dye 0.125 ulBig dye 0.25 ulBig dye 1.0 ul

Big dye 0.125 ulBig dye 0.25 ulBig dye 1.0 ul

Sequence Saver SolutionTM Eff ect of DNA SequencingTemplate: plasmid sequencing (150-200ng/ul) PCR product sequencing (10-20ng/ul) 1:3 Diluted BigDyeTM Mix (Big Dye: Dilution Buff er = 1:3)

Sequence Saver SolutionTM

Amplifi cation cycle: 30cyclesReaction volume : 10ul

Sequence Saver SolutionTM

ProductsCatalog No. Product Name Units

ISS31-B800 SEQUENCE SAVER SolutionTM /800rxns 1.6mlISS31-B32h Sequence Saver SolutionTM /3,200rxns 6.4mlISS31-B16k Sequence Saver SolutionTM /16,000rxns 32ml

to save Sequencing cost

DescriptionsSequence Saver SolutionTM is a noble reagent for economic sequencing reactions of plasmids or PCR products. A good sequencing results can be obtained with extremely small amount of BigDyeTM (0.125-0.25ul) or DYEnamicTM ET Terminator (0.25-0.5 ul) ready reaction mix by adding Sequence Saver SolutionTM into the reactions. With Sequence Saver SolutionTM the sequencing cost could be greatly reduced without compromising data quality. Sequence Saver SolutionTM is fully applicable In the BigDyeTM or DYEnamicTM ET Terminator sequencing reactions and suitable for analysis using automatic sequencers. Benefi tsSaving cost for sequencing of plasmids or PCR products.Reliable and stable results without compromising data qualityEasy adaptation to your bulk-sequencing reaction system.

QualityPlasmid(pGEM vector) sequencing

Page 12: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

DescriptionsReal-Time PCR premix is Innova Plex’s own hx-Taq DNA Polymerase. It invented as a 2X PCR premix type for convenient usage for user. Real-Time PCR premix can reduce precious study time. Just perform the PCR with DNA polymerase, dNTP, reaction buff er mixture in advance and then put included Innogreen, primer and template in the kit.

Compositions2X Real Time PCR premix 1ml*420X Innogreen 500ul*1

Quality

2X Real-Time PCR Pre-mix

ProductsCatalog No. Product Name Units

IRH 71-M40h 2X Real-Time PCR Premix (25mM MgCl2), Innogreen 500ul added 4ml

Sensitive Quantifi caiton of Target Gene

Specifi c and sensitive Quantifi cation of Target Gene

HBV strong positive control DNA serial dilution (an undiluted solution(1), 1/5, 1/10, 1/100 - standard)NTC = Negative control (No template).

(User Data)qPCR for cDNA form cold-treated Arabidopsis(Targeted to a cold -responsive gene)E** - Green dye

A1 and B1, control(untreated)A2 and B2, 3hours cold-treatedA3 and B3, 24hours cold-treated

Page 13: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Reverse Transcriptase RNase H-

DescriptionsInnova Plex Reverse Transcriptase is a recombinant enzyme purifi ed from an E.coli strain harboring a plas-mid with a part of pol gene encoding Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. The enzyme exhibits an RNA-directed DNA polymerase activity but lacks ribonuclease H activity. Innova Plex Reverse Transcriptase also contains a special thermo-stable chaperon which makes the reverse transcriptase more stable and active at high temperature.

Quality

ProductsCatalog No. Product Name Units

IOR51 E20h Reverse transcriptase(RNase H-) 10000 U

1. Total RNA 50ng2. Total RNA 25ng3. Total RNA 12.5ng4. Total RNA 6.5ng5. Total RNA 3.2ng6. Negative control (without RNA)

1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6

Innova Plex Supplier A Supplier B

Page 14: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

M-MLV Rtase(RNase-)

DescriptionsInnova Plex Reverse Transcriptase is a recombinant enzyme purifi ed from an E.coli strain harboring a plas-mid with a part of pol gene encoding Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase. The enzyme exhibits an RNA-directed DNA polymerase activity but lacks ribonuclease H activity. Innova Plex Reverse Transcriptase also contains a special thermo-stable chaperon which makes the reverse transcriptase more stable and active at high temperature.

Quality

ProductsCatalog No. Product Name Units

IMR 01 R10k M-MLV Rtase (RNase H-) 10000 U

1. Total RNA 50ng2. Total RNA 25ng3. Total RNA 12.5ng4. Total RNA 6.5ng5. Total RNA 3.2ng6. D.W

1 2 3 4 5 6 1 2 3 4 5 6 1 2 3 4 5 6

Innova Plex Supplier A Supplier B

Page 15: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

DescriptionsInnova Plex Genomic DNA Prep Kit simplifi es isolation of DNA from samples (tissue and cell) with fast spin-column. No phenol or chloroform extraction is required. DNA binds specifi cally to the Innova Plex spin-col-umn while contaminants pass through. PCR inhibitors such as divalent cations and proteins are completely removed in two simple wash steps, leaving pure DNA to be eluted in either water or buff er provided with the kit. Innova Plex DNA technology yields genomic DNA from animal tissue, plant tissue, bacteria, yeast, blood samples ready to use in PCR and blotting prodedures. The convenient spin-column procedure means that hands-on preparation time is only 25minutes. Samples can be processed using a microcenrifuge. Ap-plicable to isolation of genomic DNA from various tissue and cell samples by one single kit.

AdvantagesFast & high yieldNo phenol, no chloroformHigh quality, less fragmentationLow cost & highest servicesIsolation of gDNA from various samples by one kit

Quality

Genomic DNA Prep Kit

Animal Tissue - 50mg

Muscle Tissue Mouse Organic Tissue

Muscle Tall

Q.C test

M M 1 2 3 1 2 3 M Q I

A B C M

Q : Company Q Genomic DNA Prep KitI : Innova PlexA : Pig muscleB : Hanow muscle

1. Liver2. Kidney3. Duodenum

A: WildB: HetroC: Mutant

Q IQ I Q I

A B

1

Page 16: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

Genomic DNA Prep Kit

ProductsCatalog No. Product Name Units

IGD41-C050 Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type 50prepsIGD41-C100 Genomic DNA Prep Kit(blood, bacteria, plants, animal tissue ) - column type 100preps

Blood

Bacteria

Yeast

2

Blood Sample : 200 ul Company Q Kit Innova Plex Kit

Q.C Test

Q : Company Q Genomic DNA Prep KitI : Innova Plex

Wild type E.coli : 500ul(1X108)

Template : E.coli Genomic DNAPrimer : aroE F/R, aroK F/R

Genomic DNA separation result from Yeast(2X107 cell/ml) using Innova Genomic DNA Prep kit(2X107)

A, D : 0.5ml cel + lyticase(2.5u)B, E : 1ml cel + lyticase(2.5u)C, F : 2ml cel + lyticase(2.5u)

A : 0.5kb B : 1kbC : 2kb D : 4kbTemplate : Human gDNA

Q I Q I

aroE (0.8kb) aroK (1.7kb)

Q I

M A B C D M A B C D

M Q I

M A B C D E F M

M Q I M Q I

Page 17: Innova Plex 2008 Winter Catalog

INNOVAPLEX Tel: 832)433-7547 Tech Support: 832)372-8489 Fax: 713)574-6327 3211 Edloe St. #107 Houston, TX 77027 [email protected] http://www.innovaplex.com

AceStarTM Taq DNA Polymerase

ProductsCatalog No. Product Name Units

IDT16-R500 AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 0.4ml) 500UIDT16-R25h AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 2.0ml) 2,500UIDT16-R50h AceStarTM Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 4.0ml) 5,000U

M : 1kb* DNA LadderA : Template 10ngB : Template 2ngC : Template 0.4ngD : Template 0.08ngE : Template 0.016ngF : Template 0.008ngG : Negative (without Template)

Template : Bovine Genomic DNAM : 1kb* DNA LadderA : Enzyme 2.5unitB : Enzyme 1.25unitC : Enzyme 0.625unitD : Enzyme 0.3125unitE : Enzyme 0.15625unitF : Negative (without Enzyme)

Taq 1.25 unit AceStarTM Taq 1.25 unit

Sensitivitiy Test

Activity Test

M A B C D E F G M M A B C D E F G M

M A B C D E F M

Company G

Hs-Taq

DescriptionsInnova Plex AceStarTM Taq Polymerase was extracted and purifi ed from a cloned and modifi ed recombinant type E.Coli with DNA Polymerase gene of Thermus aquaticus. It employs Anti-Taq Antibody to exhibit advantages of both hot start PCR enzyme and general Taq Enzyme.

Benefi tsSuperior LOD, eff ecive in amplifi caiton of low copy DNAHigh activity, High sensitivityEven more eff ective than Taq DNA Polymerase in multiplex PCR condition setup.*LOD(Limit of Detection) : Method to measure amount of the minimum sample concentration needed in PCR.

Quality