Inp

ut

IgG

-L

SD

1

-H

DA

C1

Untreated SAHA

LSD1

HDAC1

Inp

ut

IgG

-L

SD

1

-H

DA

C1

IP:

(IB)

(IB)

Figure S1

Figure S1Huang, Yi et al.

Unt

reat

ed

1 M

5 M

10

M

H2O

SAHAA

LSD1

Actin

Unt

reat

ed

1 M

5 M

10

M

LSD1

Actin

B SAHA

Figure S2

Figure S2Huang, Yi et al.

Gene Function Enrichment Scoreb

Gene Namec

Mitosis, Cell division 3.08 Cyclin A2, cell division cycle associated 3, Cyclin F

RhoGAP, GTPase activation 2.01 Rho GTPase activating protein 26

G protein signaling 1.71 regulator of G-protein signaling 16, regulator of G-protein signaling 4

Negative regulation of transcription

1.69

sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), heart and neural crest derivatives expressed 1, chromobox homolog 5 (HP1 alpha homolog, Drosophila), atrophin 1, TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor, MYST histone acetyltransferase (monocytic leukemia) 4

ANK, phosphoproteins 1.68 protein phosphatase 1, regulatory (inhibitor) subunit 12B

Transcription regulator activity

1.52

nuclear receptor subfamily 4, group A (member 1), zinc finger, MIZ-type containing 2, nuclear receptor subfamily 4 (group A, member 3), pre-B-cell-leukemia homeobox 1,transcription factor EB, cAMP responsive element binding protein 3-like 1, runt-related transcription factor 1, distal-less homeobox 2, heart and neural crest derivatives expressed 1, Kruppel-like factor 2 (lung), MYST histone acetyltransferase (monocytic leukemia) 4

ATP binding, nucleotide binding

1.52 mitogen-activated protein kinase kinase kinase 13, fibroblast growth factor receptor 1, antigen identified by monoclonal antibody Ki-67

Nucleosome assembly 1.48 histone cluster 1, MYST histone acetyltransferase (monocytic leukemia) 4

Helicase activity 1.36 DNA replication helicase 2 homolog (yeast)

Transmembrane proteins 1.35 furry homolog (Drosophila), cAMP responsive element binding protein 3-like 1

Neoplasm part, polymerase II activity

1.32 TAF12 RNA polymerase II, stonin 1, TAF9B RNA polymerase II,

Endoplasmic reticulum 1.31 glycosyltransferase 25 domain containing 2

Guanyl nuclear binding 1.30 Rho family GTPase 1, ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)

Zinc ion binding 1.22

cysteine-rich protein 2, zinc finger protein 280A, ring finger protein (transmembrane 2), jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae), tripartite motif-containing 14, PDZ and LIM domain 2 (mystique)

Microtubule cytoskeleton 1.08 tubulin tyrosine ligase-like family, member 1

Protein catabolic process 1.08zer-1 homolog (C. elegans), ubiquitin specific peptidase 20, cell division cycle associated 3, ubiquitin specific peptidase 5 (isopeptidase T)

Nucleoside triphosphate metabolic process

1.06ATPase, Na+/K+ transporting, alpha 3 polypeptide, ectonucleoside triphosphate diphosphohydrolase 3, ATPase, Cu++ transporting, beta polypeptide

Table S1. Functional classification of genes whose expression is changed only by combined treatment with SAHA and pargylinea.

a. The Database for Annotation, Visualization and Integrated Discovery (DAVID) Bioinformatics Resources 6.7 program is used. b. Genes functions with enrichment score >1 are shown.c. Listed are genes with expression induction or reduction equal or greater than 1.5 fold.

Table S1Huang, Yi et al.