INSTITUTE OF PUBLIC HEALTHINSTITUTE OF PUBLIC HEALTH

Institute for Public Health of Montenegro – Centre For Disease Control and Prevention

Center for Medical MicrobiologyCenter for Medical Microbiology

Department Department Department Department Department Department

Department Department for for

bacteriologybacteriology

Department Department for for

parasitology parasitology and micologyand micology

Department Department for for

Virology Virology and and

SerologySerology

Department Department for sanitary for sanitary microbiologymicrobiology

Department Department for for

Molecular Molecular diagnosticsdiagnostics

Center for Medical MicrobiologyCenter for Medical Microbiology

Department for Department for

Virology and SerologyVirology and Serology

Serodiagnostic of

microorganisms

Department for Department for

Molecular DiagnosticsMolecular Diagnostics

Molecular diagnostic of

most important medical microorganisms

Preparing for cell culture

and virus isolation

most important medical

pathogens

•• Assoc. Prof. Assoc. Prof. BobanBoban MugoMugošša, a, responsible responsible

personpersonpersonperson

•• Assoc. Prof. Assoc. Prof. ZoranZoran VratnicaVratnica, key researcher, key researcher

•• Dr. Dr. DanijelaDanijela VujoVujošševievićć, researcher, researcher

•• Dr. Dr. SanjaSanja MedenicaMedenica, researcher, researcher

•• Dr. Dr. NebojNebojšša a SekuliSekulićć, researcher, researcher

WP1c Detection of pathogens in mosquitoes and humans

University of Montenegro

Biotechnical faculty

VISITS OF IPH RESEARCHERS TO INSTITUTE "ISTITUTO

SPERIMENTALE ZOOPROFILATTICO REGGIO EMILIA"

(IZSLER), 14 JULY - 01 AUGUST 2014

WP1c - Realized activities

Preparation of mosquitoes pools for RNA extraction;

Random primer Retro-Transcription reaction protocol

assessment;

Real Time PCR protocols and reactions set-up; Real Time PCR protocols and reactions set-up;

Real Time PCR protocols and results interpretation;

RNA extraction with semi-automatic RNA/DNA extractor.

RNA extraction

Manualy (TRIzol LS Reagent) Semi-automatic extractor

Random primer

Retro-Transcription reaction

Real Time PCR reactions set-up and

results interpretation

Results of molecular analyses from Montenegrian mosquites

Pathogen IgM

ELISA

IgG

ELISA

Microscopy Real-Time PCR

WNV 48 tests

(all negative)

48 tests

(all negative)

/ /

Borrelia burgdorferi 838 tests 840 tests / 76 tests Borrelia burgdorferi 838 tests

(79 positive)

840 tests

(59 positive)

/ 76 tests

(all negative)

Leishmania spp. / / 22

(all negative)

/

Leishmania donovani / 143 tests

(10 positive)

/ /

Plasmodium spp / / 14

(all negative)

/

INSTITUTE OF PUBLIC HEALTH MONTENEGRO INSTITUTE OF PUBLIC HEALTH MONTENEGRO

Centre for Disease Control and PreventionMandatory reporting diseases - electronic database 2014

Lyme boreliosis – six reports of disease

Leishmaniasis – three reports of disease

Infectio West Nile – no registered cases

Malaria – no registered cases

Equipment for IPH

QuantStudio™ 7 Flex Real-Time

PCR System, Applied Biosystems

ELISA Analyzer I-2P EUROIMMUN

THANK YOU!Thank you!