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InternationalConference on

Biodiversity

Abs Sem Nas Konf Masy Biodiv Indonvol. 3 | no. 2 |pp. 55-87| Mar 2016

ISSN: 2407-8069

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SECRETARIAT ADDRESS1. Sekretariat Masyarakat Biodiversitas Indonesia, Kantor Jurnal Biodiversitas, Jurusan Biologi Gd. A, Lt. 1, FMIPA UNS,

Jl. Ir. Sutami 36A Surakarta 57126, Jawa Tengah, Indonesia. Tel. +62-897-6655-281. Email: [email protected]: biodiversitas.mipa.uns.ac.id/S/2016/samarinda/home.html

2. Program Studi Biosains (S2), Program Pascasarjana Universitas Sebelas Maret (PPs UNS). Jl. Ir. Sutami 36A Surakarta57126, Jawa Tengah, Indonesia. Tel./fax.: +62-271-632450

Organized by Selected manuscriptswill be available at

TIME SCHEDULEInternational Conference on Biodiversity

Society for Indonesian Biodiversity (SIB)Yogyakarta, Indonesia, 19-20 March 2016

TIME ACTIVITIES PERSON IN CHARGE SITE

March 19, 2016

08.00-09.00 Registration Committee Lobby09.00-09.10 Speech of the Committee Chairman of the committee R109.10-09.20 Opening speech Chairman of the society R109.20-09.30 Photo Session and Coffee Break Committee R1,

Lobby09.30-11.00 Panel 1 Moderator R1

Prof. Dr. SutarnoProf. Dr. Triwibowo Yuwono

11.00-12.30 Panel 2 Moderator R1Prof. Dr. Johan IskandarDr. Jean W.H. Yong

12.30-13.30 Rest, prayer, lunchPoster session

Committee Lobby

13.30-14.30 Parallel presentation IGroup 1: AO-01, AO-02, AO-03, AO-04, AO-05, AO-06 Moderator R1Group 2: AO-07, AO-08, BO-01, BO-02, BO-03, BO-04 Moderator R2Group 3: BO-05, BO-06, BO-07, BO-08, BO-09, BO-10 Moderator R3Group 4: BO-11, BO-12, BO-13, BO-14, BO-15, BO-16 Moderator R4Group 5: BO-17, BO-18, BO-19, BO-20, BO-21, BO-22 Moderator R5

14.30-14.45 Coffee Break Moderator Lobby

14.45-15.45 Parallel presentation IIGroup 6: CO-01, CO-02, CO-03, CO-04, CO-05 Moderator R1Group 7: CO-06, CO-07, CO-08, CO-09, CO-10 Moderator R2Group 8: CO-11, DO-01, DO-02, DO-03, EO-01 Moderator R3Group 9: EO-02, EO-03, EO-04, EO-05, EO-06 Moderator R4Group 10: EO-07, EO-08, EO-09, EO-10, EO-11, EO-12 Moderator R5

15.45-16.00 Closing speech and other explanations Chairman of the committee R1

Upcoming events:1. Seminar Nasional Masyarakat Biodiversitas Indonesia, Padang, Indonesia, 23 April 20162. International Conference on Biodiversity, Bandung, Indonesia, 28-29 May 2016

TABLE OF CONTENTSInternational Conference on Biodiversity


CODE TITLE AUTHOR(S) PAGES

Genetic diversityAO-01 Genetic diversity of sweetpotato in the Arfak Mountain

Region, West PapuaNouke L. Mawikere, SaraswatiPrabawardani, Imam Widodo,Yohanes Mustamu

55

AO-02 Genetic diversity and conservation strategyconsiderations for highly valuable medicinal tree ofTaxus sumatrana in Indonesia

Henti Hendalastuti Rachmat, AtokSubiakto, Koichi Kamiya

55

AO-03 Vegetative and generative performance of groundnutgenotyprs under biotic stress environments

Agustina Asri Rahmianna,Eriyanto Yusnawan, JokoPurnomo

56

AO-04 The role of Dendrobium Orchid Homeobox 1 (DOH1)gene during in vitro shoot development of wild orchidDendrobium lineale Rolfe

Endang Semiarti, Wahyu DewiAstuti Ningrum

56

AO-05 Genetic variety of local varieties of rice South Sumatrabased on Polymerase Chain Reaction-RandomAmplified Polymorphic DNA (PCR-RAPD)

Yuanita Windusari, Laila Hanum,Muharni, Fikri Adriansyah

57

AO-06 Genetic variation and phylogenetic tree of Couraambonensis in Central Indonesia based on cytochrome Bgene sequence

Mohamad Amin, Endang Suarsini,Umie Lestari, Abdul Gofur, DwiMartha Nur Aditya, Devan AdityaRahman, Candra Hermawan

57

AO-07 Plant collecton in South Sulawesi for sugarcaneimprovement

Bambang Heliyanto, Untung SetyoBudi

57

AO-08 Screening, characterization of endo-xylanase enzyme asaccessory enzyme to enhance of sugar cane baggasesaccharification in biorefineries applications

Nanik Rahmani, Pamela Apriliana,Alifah M. Jannah, PuspitaLisdiyanti, Yopi, BambangPrasetya, Jaemin Lee, PrihardiKahar, Chiaki Ogino, AkihikoKondo

58

AP-01 The diversity of local cattle in Indonesia and the effortsto develop superior indigenous cattle breeds

Sutarno, Ahmad Dwi Setyawan 58

AP-02 The performance of agronomic character and diversityfrom 62 accession of sweet potato germplasm

Wiwit Rahajeng, St. A.Rahayuningsih

59

vi

AP-03 Genetic diversity of sun-flower (Helianthus annuus)germplasm based on quantitative and qualitativemorphological characters

Rully Dyah Purwati, Anik Herwati 59

AP-04 The effect of mutations induced by gamma-rayirradiation to the performance of Reutealis trisperma.

Rully Dyah Purwati, Parnidi,Aprilia Ridawati, MalaMurianingrum

59

AP-05 Response of 14 soybean genotypes to whiteflies(Bemisia tabaci): Antixenosis, antibiosis, and tolerance

Apri Sulistyo, Alfi Inayati 60

AP-06 The response of soybean genotypes introduced fromSouth Korea to drought stress during the reproductivestage

Suhartina, Novita Nugrahaeni,Apri Sulistyo

60

AP-07 Improvement of genetic variability in seedlings ofSpathoglottis plicata orchid through X-ray irradiation

Suyitno Aloysius, AzizPurwantoro, Kumala Dewi,Endang Semiarti,

61

AP-08 The study of soybean genotipes response againts Asiansoybean rust (ASR)

Alfi Inayati, Eriyanto Yusnawan 61

AP-09 Genetic relationship among Indonesian mungbeangermplasm based on quantitative traits

Ratri Tri Hapsari, Trustinah, RudiIswanto

61

AP-10 Phenotypic test and identification of microsatellitemarkers related to aluminium tolerance in upland rice

Yuliana Galih Dyan Anggraheni,Sri Indrayani, Enung SriMulyaningsih

62

Diversity of speciesBO-01 The impact of deforestation rate for extinction

biodiversity in IndonesiaI Putu Gede Ardhana 62

BO-02 Lichen: Comparative study between Campus Area ofUniversitas Muhammadiyah Surakarta and forest ofSekipan Karanganyar, Central Java

Efri Roziaty 62

BO-03 Preliminary test of Agri-Environmental Schemeadoption in farmland of northern slope of Mount Slamet,Central Java, Indonesia

Imam Widhiono, Eming Sudiana,Edy Tri Sucianto

63

BO-04 Bioaccumulation of copper by microorganisms isolatedfrom industrial sewage in Rungkut-Surabaya, Indonesia

Wahyu Irawati, Salomo Christian,Triwibowo Yuwono

63

BO-05 Diversity of parasitic plants and their hosts inhomegardens agroforestry in Kepala Jeri and PempingIslang, Batam

Solikin 63

BO-06 The diversity and distribution of Holothuroidea inshallow waters of Baluran National Park, Indonesia

Arif Mohammad Siddiq, TriAtmowidi, Ibnul Qayim

64

BO-07 Methanolic extracts of Ageratum conyzoides,Amaranthus spinosus and Cyperus rotundus as botanicalfungicides to control peanut rust disease

Eriyanto Yusnawan, Alfi Inayati 64

BO-08 Pattern of insect community associated with Santalumalbum

Arina Damayanti, Ananto Tiyogo,Yeni Widyana NR

64

BO-09 Natural regeneration of burnt peat swamp forest andburnt peat land in core area Giam Siak Kecil Bukit BatuBiosphere Reserve Riau Province and Its implication onconservation

Elfis, Prima Wahyu Titisari 65

BO-10 Mangrove succession in the restoration areas ofSembilang National Park, South Sumatra

Sarno, Harmida, Nita Aminasih,Hanifa Marisa and Ranti Seprina

65

vii

BO-11 Assosiations of epiphytic microalgae on macroalgaewith macroalgae thallus form in Muara Binuangeun ReefFlat, Lebak, Banten

Aprilian Pryski Waskitho Adi, TitiSoedjiarti, Riani Widiarti

65

BO-12 The aviability of feed plants resources of Jalak Lawu(Turdus poliocephalus stresemanni Bartels.) in MountLawu

Muhammad Ridwan, Suratman,Sugiyarto

66

BO-13 Plants diversity of the deforested peat swamp forest ofTripa, Indonesia

Djufri, Wardiah, Z.A. Muchlisin 66

BO-14 Total phenolic, flavonoid content and antioxidantactivity of cultivated tabat barito (Ficus deltoidea) onvarious level on age

Hetty Manurung, WawanKustiawan, Irawan W. Kusuma,Marjenah

66

BO-15 The density, composition, and mangrove forest habitatsin coastal areas of Torosiaje Jaya Village of PopayatoDistrict, Gorontalo Province

Sukirman Rahim, Dewi WahyuniK. Baderan, Marini SusantiHamidun,

67

BO-16 Effect of age differences in the presence of dung beetles Etik Susanti, Ardita Tri AnugrahBudaya, Dian Sartika, R.C.Hidayat Soesilohadi

67

BO-17 Macroinvertebrate diversity in Water QualityAssessment of Winongo and Gajah Wong Rivers,Yogyakarta Special Region

Amelia Nugrahanigrum, MartinaFaika Harianja, HendriawanNugroho, R.C. HidayatSoesilohadi

68

BO-18 Diversity of morphological and physioly endophyticentomopathogenic fungi from peanut plant

Novri Nelly, Trizelia, Reflinaldon 68

BO-19 Odonata diversity in Rawa Bendungan, Cilacap, CentralJava

Muhammad Nu'manuddin 68

BO-20 Some species of the Genera Cobbonchus Andrassy,1958 and Mylonchulus Cobb, 1916 (Nematoda:Mononchida) from South Kalimantan

Abdul Gafur 68

BO-21 Georeferencing orchids specimen history cards in BogorBotanic Gardens to increase their use for conservationefforts

Eka Martha Della Rahayu, SafranYusri

69

BO-22 Rediscovery of Fagraea litoralis (Loganiaceae) inMount Nglanggeran of Baturagung MountainsYogyakarta

Widodo, Muhammad Ja'far Luthfi 69

BP-01 Existence of bats in Mount Walat Education Forest,Sukabumi, West Java

Adheliya Setyorini, Sasti RegiBintari

69

BP-02 Early growth of three Dipterocarpaceace species underrubber plantation in North Bengkulu District, BengkuluProvince

Wiryono, Hery Suhartoho, AliMunawar

70

BP-03 Variability on morphological characters of podassociated with pod shattering resistance in soybean

Ayda Krisnawati, M. MuchlishAdie

70

BP-04 Variability of pod trichome’s density and length onseveral soybean genotypes

M. Muchlish Adie, AydaKrisnawati

70

BP-05 The diversity and kinship of the swamp buffalo (Buffalobubalis) Pampangan South Sumatra based on thecharacteristics morphologists

Yuanita Windusari, Laila Hanum,Rahmat Pratama

71

BP-06 Variability of lateral shoot and root formation of in vitroculture of sengon (Paraserianthes falcataria)

N. Sri Hartati, Dody Priadi 71

BP-07 Development potential of Citrus cv. Nimas Agrihorti asbiopharmacy citrus

Emi Budiyati, Joko Susilo Utomo,Anis Andrini

71

viii

BP-08 Typical of mangrove forests in lagoon areas of southerncoast of Java

Ahmad Dwi Setyawan, AriSusilowati, Ari Pitoyo, Jean W.H.Yong

72

Diversity of ecosystemCO-01 The roles of community fruit garden (tembawang) on

maintaining vegetation structure, diversity and standingbiomass allocation: an effort on reducing carbonemission

Dwi Astiani, Ripin 72

CO-02 Choosing native tree species for establishing man-madeforest: A new perspective for sustainable forestmanagement in changing world

Atok Subiakto, Henti HendalastutiRachmat,

72

CO-03 The diversity of plant species, the type of plant uses andthe estimate of carbon stock in agroforestry system inHarapan Makmur Village, Pondok Kubang Sub-district,Central Bengkulu District

Wiryono, Venny Utami Puteri,Gunggung Senoaji

73

CO-04 Diversity of faunal communities in the Biodiversity Parkof Aqua Danone Ciherang, Bogor, West Java

Hendra Gunawan, Sugiarti, AnitaRianti, Vivin SilvaliandraSihombing

73

CO-05 Ex situ conservation of North Sumatran Mountain Floraat Samosir Botanic Garden

Sugiarti 74

CO-06 Climate-induced hydrological changes and the ecologyof freshwater Biota: A review

Sunardi, Gerhard Wiegleb 74

CO-07 Status of population, occupation and seasonal habitatdisplacement of alien bird species in West Java tropicalforest

Ruhyat Partasasmita 74

CO-08 Biological treatment with consortia of Mn2+ and Fe2+

oxidizing bacteria from alfisol Jumantono forgroundwater purification

Vita Ratri Cahyani, Sumani, DwiPriyo Ariyanto, Ayu Indah Saputri

75

CO-09 Effect of arbuscular mycorrhizal inoculum originatedfrom Surakarta Area and rock phosphate toward Puptake of corn on alfisol Jumantono

Vita Ratri Cahyani, Sudadi, DiyahRatri Palupi

75

CO-10 Mangrove health index as part of sustainablemanagement in mangrove ecosystem at KarimunjawaNational Marine Park, Central Java

Johan Danu Prasetya,Ambariyanto, Supri Haryono,Frida Purwanti

75

CO-11 Indigenous Kaliwu agro-ecosystem, biodiversityconservation and environmental service on SumbaIsland, East Nusa Tenggara

Gerson N. Njurumana 76

CP-01 Isolation of Actinomycetes from mangrove ecosystem inTorosiaje, Gorontalo

Abubakar Sidik Katili, YulianaRetnowati

76

CP-02 Farmer adaptation strategy in paddy field affected byclimate variability in monsoon regions

Yayan Apriyana, Yeli Sarvina,Elsa Rakhmi Dewi, and ArisPramudia

77

CP-03 Management leadership in the promotion of villagefarmer softskill information technology in the Provinceof Gorontalo

Novianty Djafri 77

ix

EthnobiologyDO-01 The local knowledge of the rural people on species, role

and hunting of birds: Case study in KarangwangiVillage, Cidaun Sub-district, Cianjur, West Java

Johan Iskandar, BudiawatiSupangkat Iskandar, RuhyatPartasasmita,

77

DO-02 Ethnobotany of traditional healer (dukun) in Giam SiakKecil Bukit Batu Biosphere Reserve Riau Province

Prima Wahyu Titisari, Tati SuryatiSyamsudin, Achmad Sjarmidi,

78

DO-03 Nature conservation through local wisdom: the role ofsacred places in western slope of Mount Lawu inpreserving rare trees

Nor Liza, Muhammad ArifRomadlon, Muhammad Ridwan,Anisa Septiasari, Irmayanti SariDewi, Zenita Mila Lutfia, KrisantyKharismamurti, Dafi Al. Anshori,Rizma Dera Anggraini, RekyanGaluh Witantri, Dwi SetyoSaputro, Yudha Noviana

78

BioscienceEO-01 Effects of paclobutrazol on growth and yield of several

potato varieties grown at mid elevation areaKumala Dewi, FransiscusXaverius Wagiman, DidikIndradewa, Erlin Kurniati

79

EO-02 Introduced new forages species to sustain biodiversity incoconut based farming

David A. Kaligis, Selvie D. Anis,Malcky M. Telleng

79

EO-03 Stability of rhizobacterial isolate in some formulas toincrease growth and yield of soybean in the field

Trimurti Habazar, Yulmira Yanti,Zurai Resti, Julio Eifelt Rumbiak

80

EO-04 The soil improvement potential of weeds in marginalland

Anti Damayanti Hamdani, EkaSulistiyowati, Arifah Khusnuryani

80

EO-05 Identification the components of fatty acid compoundson the seed of Leucaena leucocephala L. as the potentialsources of halal lecithin base materials

Ika Nugraheni Ari Maartiwi 80

EO-06 Effect of genotype on sporofit formation from prothallusmass of the golden chicken fern (Cibotium barometz) invitro

Yupi Isnaini, Titien NgatinemPraptosuwiryo

81

EO-07 Production of β-Mannanase on porang potato byBacillus subtilis LBF-005 and its potential for manno-oligosaccharide production

Yopi, Nanik Rahmani, AlifahMafatikhul Jannah, Irfan PebiNugraha, Roni Masri Ramadana

81

EO-08 Energy pathway in soil nematode food web underdifferent cropping pattern of maize and legumes crop

Laode Muhammad HarjoniKilowasid, Laode Sabarudin,Lisnawati, Hasbullah Syaf, GustiRay Sadimantara, Syamsu Alam,Namriah, Mardin

81

EO-09 Morphological variations and sex expression ofgametophyte of Cibotium barometz under in vitroconditions

Titien Ngatinem Praptosuwiryo,Yupi Isnaini

82

EO-10 Pathogenicity of entomopathogenic fungus Metarhiziumspp. against predators Menochilus sexmaculatusFabricius (Coleoptera: Coccinelidae)

Trizelia, Munzir Busniah, AgungPermadi

82

EO-11 Antioxidant potency of etanolic extracts of callusdeveloped from carrot and tomato

Rumiyati, Sismindari, EndangSemiarti, Sekar Galuh, ChristineAlfiani Hana

82

x

EO-12 Study of gamma irradiation on the creating of mutant oflocal rice (Oryza sativa) mentik susu variety

Ahmad Yunus, Samanhudi,Parjanto, Susilo HambegPoromarto, Dyan Farisa

83

EP-01 Expression of Wnt4 gene in the Swiss Webster miceuterus as candidate antigen for wildlifeimmunocontraception method

Agung Janika Sitasiwi, WayanTunas Artama, Agung Budiyanto,Edi Dharmana

83

EP-02 The influence of the media Limboto lake sediment onthe growth of maize were inoculated arbuscularmycorrhizal

Novri Youla Kandowangko, JusnaAhmad, Fujiawaty, Asni SantiPatilima

83

EP-03 Effects of compost type and rootstock length on growthresponse of fruit seedlings and vegetable plants in thenursery

Dody Priadi, Enung SriMulyaningsih

84

EP-04 Correlation of pod characteristics with pod borrer Etiellazinckenella Treitschke preference

Kurnia Paramita S., Suharsono, M.Muchlish Adie

84

EP-05 Effect of five host plants on the growth and developmentof armyworm, Spodoptera litura

Marida Santi Yudha Ika Bayu,Ayda Krisnawati

84

EP-06 Mycorrhizal population on various cropping patterns onsandy soil in dryland area of North Lombok, Indonesia

Wahyu Astiko, Muhammad TaufikFauzi, Sukartono

85

EP-07 Isolation and characterization of mannanase, xylanaseand cellulase from marine bacteria Bacillus sp.

Yopi, Apridah CameliawatiDjohan, Nanik Rahmani, AlifahMafatikhul Jannah

85

EP-08 In vitro embryo development of a wild orchidDendrobium phalaenopsis as an efficient method for exsitu conservation

Nintya Setiari, Aziz Purwantoro,Sukarti Moeljopawiro, EndangSemiarti,

85

EP-09 Construction of 35S::PaFT1::GFP containing Ti plasmidin Agrobacterium tumefaciens EHA101 for genetictransformation in plant

Sri Wahyuningsih, MuhammadDylan Lawrie, Budi SetiadiDaryono, Sukarti Moeljopawiro,Endang Semiarti

86

EP-10 Green pod culture of variegated-Phalaenopsis "SogoVivien" orchid

Exsyupransia Mursyanti , Aziz-Purwantoro, SukartiMoeljopawiro,Endang Semiarti

86

EP-11 Study of cross inoculation of Rhizobium tropici (DCM)isolated from Acacia mangium in combination withother potential soil microbes on their ability to supportthe growth of soybean

Sylvia Lekatompessy, LiseuNurjanah, Harmastini Sukiman

87

EP-12 Fecundity performance of nile tilapias (Osteochilushasselti) from different districts in West Java

Rita Rostika, Yuli Andriani,Junianto

87

Note: A. Genetic Diversity, B. Diversity of Species, C. Diversity of Ecosystem, D. Ethnobiology, E. Bioscience (LifeScience and Technology); O. Oral, P. Poster

ABS SEM NAS KONF INTL MASY BIODIV INDONVolume 3, Nomor 2, Maret 2016 ISSN: 2407-8069Halaman: 55-87 DOI: 10.13057/asnmbi/m030201

ABSTRACTInternational Conference on Biodiversity


Genetic diversity

AO-01Genetic diversity of sweetpotato in the ArfakMountain Region, West Papua

Nouke L. Mawikere, Saraswati Prabawardani♥, ImamWidodo, Yohanes MustamuFaculty of Agriculture, State University of Papua. Jl. Gunung Salju,Amban, Manokwari 98314, West Papua, Indonesia. ♥email:[email protected]

Major factors that constitute a threat to food security isshifting Papuan diet from sweetpotato and sago to rice.This situation is exacerbated by implementation of thegovernment program so called "Rice for Poor (Raskin)".The dependency on rice as what has been seen today is notfavorable for food security in this region. Therefore,communities’ awareness in maintaining the diversity oflocal food is needed for food security in this region. Theresearch was conducted to achieve the followingobjectives: (i) Documenting the diversity of sweetpotato,its utilization, and overall cultivar frequency (OCF) andrelative cultivar frequency (RCF) in the Arfak MountainRegions, as well as gender knowledge towards sweetpotatocultivars, (ii) Identifying the trends in the use of cultivars,especially the overall decline and/or the frequency shiftrelative to other cultivars or other food crops, (iii)Determine the level of intervention that can stabilize thecurrent sweetpotato diversity through the use of alternativeand improving recognition of their contribution to thelandscape tough. The study was conducted in 2012 inMinyambouw, Hink and Anggi districts of Arfak MountainRegion. Method of this study consisted of (i)Documentation of the community diversity level (CDD),collection and documentation of sweetpotato cultivarsexisted in the village or other social groups through groupmeeting. Meeting was previously planned and was carriedout by invited farmers. Farmers were asked to bring allsweetpotato cultivars which are planted in their gardens (ii)Mapping RCF was done by counting the number of

sweetpotato per sweetpotato cultivar at each plot. Based onthe study, sweetpotato diversity in the Arfak Mountains isstill relatively high. Based on documentation study of thecommunity-scale (sweetpotato Exhibition): Hink Districthas the highest number of sweet potato cultivars (50cultivars) compared to Anggi District (30 cultivars), andMinyambouw District (15 cultivars). Sweetpotato cultivarsthat have the highest OCF or brought by many participantsare Syeb Nggrai and Syeb Ngoi and S. Simpeungei(Minyambouw), Bkau Ngoi (Hink), and Sweet Aug.(Anggi). The morphological characteristics of tubers andleaves of all sweetpotato cultivars were varied widely.There were several cultivars that did not longer planted bypeople in Arfak Mountains, such as Syeb Nipon, SyebSinong, and Syeb Snoggau in Minyambouw, Hiba andBkaw Bkaw Braba in Hink, and Aug. Tug, Aug Bocop, andAug Birerec in Anggi. In the selection of seeds for the nextsweetpotato cultivation, the Arfak people prefer plantingmaterials from their own garden. Vine cutting is used asplanting material for sweetpotato cultivation.

Arfak, food security, genetic diversity, sweet potato

AO-02Genetic diversity and conservation strategyconsiderations for highly valuable medicinal treeof Taxus sumatrana in Indonesia

Henti Hendalastuti Rachmat1,♥, Atok Subiakto2,♥ ♥,Koichi Kamiya3

1 Forest Fiber Technology for Research Plantation. Jl. Raya Bangkinang-Kuok Km 9, Kotak Pos 4/BKN Bangkinang 28401, Riau, Indonesia. Tel.:+62-762-7000121, ♥email: [email protected] Forest Research and Development Center, Ministry of Environmentaland Forestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, JawaBarat. Tel. +62-251-8633234; 7520067. Fax. +62-251 8638111. ♥♥email:[email protected] Faculty of Agriculture-Ehime University, 3-5-7 Tarumi, Matsuyama,Ehime Pref. 790-8566, Japan.

Genetic variation is considered to be the key factor forlong-term survival of the species. The recognition of the

ABS NAT SEM INTL CONF SOC INDON BIODIV, Yogyakarta, 19-20 Maret 2016, hal. 55-8756

existing genetic diversity is the preliminary phase indevelopment of an effective strategy for conservation offorest tree species. Taxus sumatrana is confined to grownaturally only in Asia, it is a rare and endangered speciesthat in several Asian countries needs both ex situ and insitu protection program. In its natural distribution, T.sumatrana is the only Taxus species that reaching itssouthernmost distribution to Sumatran forest-Indonesia.The objective of this research was to determine the geneticvariation of T. sumatrana as baseline information fordesigning conservation strategy of the species. Leavessamples were collected from two natural population of T.sumatrana in Mt. Kerinci (Sungai Penuh-Jambi) and Mt.Dempo (Pagaralam-South Sumatra), both sites arestretched along Bukit Barisan Mountain Ranges ofSumatra. We sequenced two non-coding chloroplast DNAregions of trnL-trnFI and psbC-trnS that each yielded 808bp and 1092 bp, and rbcL gene of 523 bp, in which thetotal length covered 2423 bp. Surprisingly, we found novariation for all individuals and population, which meansthat the species is similar and both populations are havingno structuring. This study also revealed on how a properconservation strategy should be practiced for the species aswe know that without a sufficient amount of geneticvariation, a population cannot evolve in response tochanging environmental conditions. In situ conservationprogram is a must that can maintain the existence of thespecies while at the same time keeping the sustainability ofthe entire systems; in other side ex-situ conservationstrategy can take place as an additional effort to secure thegenetic resources in case of the catastrophic events thatmight diminish their limited natural habitat.

Conservation strategy, cpDNA, genetic diversity, rbcL gene,Taxus sumatrana

AO-03Vegetative and generative performance ofgroundnut genotyprs under biotic stressenvironments

Agustina Asri Rahmianna♥, Eriyanto Yusnawan, JokoPurnomoIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

The ultimate biotic factors that limited yields are pests,diseases and weeds. Among these three factors, pod yieldof groundnut is mainly influenced by disease infestation,especially bacterial wilt and foliar diseases. The experimentwas aimed to determine the response and tolerance ofgroundnut genotypes to bacterial wilt, leaf spot and rustdiseases, and seed infection by Aspergillus flavus. Theplanting materials were 25 genotypes (11 Indonesiacultivars, 12 lines introduced from ICRISAT, 1 Indonesiapromising line, and 1 local variety) with varioussuperiorities on diseases resistance. A randomized

completely block design with 3 replicates was applied. Theplant spacing was 40 x 15 cm, 1 plant/hole. The basalfertilizers of 22.5 kg N, 36 kg P2O5 and 50 kg K2O/hawere applied at sowing. Crop maintenance was intensivelyundertaken. The results indicated that both genotypes fromICRISAT and Indonesia had similar response to leaf spoti.e. ranging from susceptible (score 6-7) to highlysusceptible (8-9). The score for rust ranged frommoderately resistant to susceptible. The average pod yieldwas 23.1 g/plant (from 11.9 g to 29.5 g), and 13 and 12genotypes produced pods higher and lower than theaverage value, respectively. The highest and lowest seedyields were 19.0 g and 6.9 g, with 14 higher and 11genotypes gave higher and lower seed yield than theaverage (13.3 g). ICGV 86158 and ICGV 95322 had thehighest and lowest seed as well as pod productivity,respectively. The ICRISAT genotypes were susceptible toRalstonia solanacearum infection, except ICGV 86590.Among the Indonesia cultivars, those with Valencia type ofgrowth, relatively had better resistance to bacterialinfection. These cultivars were also resistant to rust and A.flavus infection.

Biotic stress, generative, groundnut, vegetative performance

AO-04The role of Dendrobium Orchid Homeobox 1(DOH1) gene during in vitro shoot development ofwild orchid Dendrobium lineale Rolfe

Endang Semiarti♥, Wahyu Dewi Astuti NingrumFaculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.Tel/Fax. +62-274-580839,♥email: [email protected]

Dendrobium lineale Rolfe is a wild orchid of Papua withmany fragrant flowers. Plant propagation through in vitroculture techniques is the best method for ex situconservation of this endemic orchid. The objective of thisstudy was to analyze shoot development from embryo of D.lineale related to the involvement of Dendrobium OrchidHomeobox 1 (DOH1) during in vitro culture. This studywas conducted in two steps: 1). Seed germination of D.lineale in VW medium with and without addition of 2 g/LPeptone to produce protocorms (developing orchidembryo); and 2) Analysis of the expression of DOH1 geneduring in vitro development of D. lineale protocorms. Theresults showed that embryo development of D. lineale weredivided into six phases. Addition of 2 g/L peptone in theculture medium accelerated and increased the percenteageof seed germination up to 100%, enlarged the protocormsize, greenish the protocorm and shoot initiation. Molecularanalysis showed that the DOH1 cDNA with a size of 1.2 kbstart can be detected earlier in protocorm aged 3 weeksafter sowing (WAS) that grown in medium supplementedwith peptone than that on basic VW medium, and at the ageof 5 WAS the expression of DOH1 can be detected in alltreatments. The most effective medium for seedgermination and shoot induction in vitro is VW + peptone.

ABS SEM NAS KONF INTL MASY BIODIV INDON, Yogyakarta, 19-20 March 2016, hal. 55-87 57

These data shows that the activity of DOH1 gene initiateshoot development from protocorm during in vitro cultureand can be accelerated by addition of peptone.

Culture medium, Dendrobium lineale, DOH1, peptone,shoot induction

AO-05Genetic variety of local varieties of rice SouthSumatra based on Polymerase Chain Reaction-Random Amplified Polymorphic DNA (PCR-RAPD)

Yuanita Windusari♥, Laila Hanum, Muharni, FikriAdriansyahBiology Department, Faculty of Mathematics and Natural Sciences,Sriwijaya University. Jl. Palembang-Prabumulih Km 32, Indralaya, OganIlir 30662, South Sumatra, Indonesia. ♥email: [email protected]

South Sumatra is rich of germ plasma with local varietiesof rice and high-yielding varieties. The existence of localvarieties of rice is in danger. This is due to the introductionof high-yielding varieties of rice which causes graduallythe local varieties of rice abandoned by the rice farmers.Superior properties owned by local varieties of rice arestored in the genes. Genetic variation in plant breedingactivities are important as an information basis for plantbreeding. This research aims to determine genetic varietyof 22 local varietie of rice South Sumatra based on PCR-RAPD. this research has been done on August 2015 toDecember 2015, at The Laboratory of Microbiology andthe Laboratory of Genetics and Biotechnology,Departement of Biology, Faculty of Mathematics andNaturaal Science, Sriwijaya University, South Sumatra,Indonesia. Obtained as many as 22 local varieties of ricesamples collected from the South Sumatra in collection ofpopulation from Ogan Ilir district, Kelingi Muara district,Banyuasin district, Musi Rawas district, Muara Enimdistrict and Ogan Komering Ilir district. Genetic variationsidentified using PCR-RAPD techniques. 7 primers (OPA,OPA-9, OPA-10, OPA-13, OPA-16, OPA-19, OPB-8)were used for amplification. Based on the research that hasbeen done, all primers can produce polymorphic band witha total of 70 polymorphic DNA bands (100 bp 900 bp)53.6%. This indicates a high genetic variation. Primer mostpolymorphic DNA fragments that OPA-13, OPA-19, andOPB-8. Additionally obtained also specific DNA bands ofprimer OPA-3 (100 bp) Dayang Telasih accession, primerOPA-9 (375 bp) Dayang Kuning accession, OPA-13 (700bp) Panak/Pendek accession, DNA bands indicate thepresence of specific polymorphisms properties.

Genetic variety, local varieties, PCR-RAPD

AO-06Genetic variation and phylogenetic tree of Couraambonensis in Central Indonesia based oncytochrome B gene sequence

Mohamad Amin♥, Endang Suarsini, Umie Lestari,Abdul Gofur, Dwi Martha Nur Aditya, Devan AdityaRahman, Candra HermawanBiology Department, Faculty of Mathematics and Natural Sciences, StateUniversity of Malang. Jl. Semarang No. 5, Malang 651145, East Java,Indonesia. Tel./fax.: +62-341-562180. ♥email:[email protected]

The population of Cuora amboinensis which spread overcentral Indonesia area is decreased. Analysis of geneticvariation and phylogeny is one effort to conservation focusthrough prediction the genetic diversity of these population.Mitochondrial DNA is one of the commonly usedmolecular markers to study it. The purpose of this researchthat revealed the genetic diversity and phylogeny of C.amboinensis based on cytochrome b gene sequences.Descriptive exploratory study was carried out to obtaingene sequences of mitochondrial cytochrome b. Thesample of C. amboinensis was collected from Ambon,Kendari, Gorontalo, Luwu, Toli-Toli, and Sangir area. Asan out group sequence is used cytochrome b genesequences from Cuora trifasciata. The series of researchactivities include: DNA isolation, DNA purification,polymerase chain reaction (PCR), horizontalelectrophoresis, sequencing, data analysis with MEGA 5.21software. Based on the data analysis showed that thephylogenic tree of C. amboinensis in this study is closelyrelated. The values of genetic distance ranged ≤ 0.30, andthe value of similarity and variants on average by 82.5%.The conclusion of this study is the phylogeny of C.amboinensis that distributed in central Indonesia is closelyrelated, because the value of genetic variations too small.

Coura ambonensis, cytochrome b gene, genetic variation,phylogenetic tree

AO-07Plant collecton in South Sulawesi for sugarcaneimprovement

Bambang Heliyanto♥, Untung Setyo BudiIndonesian Sweetener and Fiber Crops Research Institute (ISFCRI). Jl.Raya Karangploso 199, Malang 65152, East Java, Indonesia. Tel.: +62-341-491447, Fax.: +62-341-485121. ♥email: [email protected]

Success of any crop breeding program is depended uponthe extent of genetic variability present in the germplasmholding; the higher the extent of genetic variation thehigher the chances of success. At present, sugarcanegermplasm collection at the Indonesian Sweetened andFibre Crop Research Institute (ISFRI), the currentgovernment institute for sugarcane R & D, is very limited.To widen the genetic base of the crop, incountrygermplasm collecting mission was mobilized to Kendari


District, Muna and Buton Districts, South East SulawesiProvinc, from 9 July to 19 July 2012. The collector team,lead by ISFCRI’s scientist, and supported by local EstateCrop office, had successfully collected a total of 66accessions consisted of 62 Saccharum oficinarum; 4accession of S. edule; Morphological variability were seenin term of stalk color, stem wax and eye shape. To assessfurther their genetic potential, these newly collectedgermplasm are being planted at Ngemplak ExperimentalGarden, Pati.

Exploration, germplasm, Sacharum edule, S. officinarum, S.spontaneum, Kendari variability

AO-08Screening, characterization of endo-xylanaseenzyme as accessory enzyme to enhance of sugarcane baggase saccharification in biorefineriesapplications

Nanik Rahmani1,♥, Pamela Apriliana1, Alifah M.Jannah1, Puspita Lisdiyanti1, Yopi1, BambangPrasetya1, Jaemin Lee2, Prihardi Kahar2, ChiakiOgino2, Akihiko Kondo2

1 Research Center for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected] of Chemical and Engineering, Graduate School ofEngineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan

Development of second-generation of biofuels technologywhich produced from lignocellulosic biomass becomes theresearch focus in the world. One method that cansignificantly increase the efficiency of converting biomassinto bioethanol is consolidated bioprocessing (CBP). Theutilization of enzyme in CBP is very expensive, so for thedevelopment of Bio-refinery, the reduction cost of enzymesare very important. We need a strategy that makes thebiorefinary process more economical by improving theperformance of the enzyme. Screening and characterizationof novel bacteria capable of producing high level cellulaseand hemicellulose seem to be all suited starting point forobtaining improved cellulose and hemicellulose hydrolysisvia boosting of co-adjuvant enzymes. The objective of thisresearch are screening and characterization of novelbacteria from Biotechnology Culture Collection (BTCC)capable of producing high level hemicellulase-degradingenzymes, especially for endo-xylanase enzyme. Using acollection of local microorganisms and its geneticresources, combined CBP technologies is considered to bea good solution to improve the process to make it moreefficient for utilization of lignocellulosic biomasses for theproduction of bioethanol. We have been screening morethan 797 isolates from Indonesia Actinomycetes by usingcongo red, SDS-PAGE and zymogram methods and getseven isolates capable producing high level of xylanaseenzymes based on the diameter of clear zone more than 1.5

cm on xylan plate media pH 5 and 6. Some isolates wereidentified as a Streptomyces sp and Kitasatospora sp. Thereare six isolates have ability to produce xylanase optimum atpH 5, one isolate pH 6 and temperature range 50-70 oCwith range activity from 1.3-5.8 U/mL. Each isolate hasmolecular weight from 20-50 kDa based on SDS-PAGEand zymogram analysis. From these characterizationshowed that these isolates potential used forsaccharification of sugar cane bagasse and oil palm emptyfruit bunch (OPEFB) by consolidated bioprocessing (CBP)for biorefinary applications.

Biotechnology Culture Collection (BTCC), consolidatedbioprocessing (CBP), endo-xylanase, hemicellulase,saccharification

AP-01The diversity of local cattle in Indonesia and theefforts to develop superior indigenous cattlebreeds

Sutarno, Ahmad Dwi SetyawanDepartment of Biology, Faculty of Mathematics and Natural Sciences,Sebelas Maret University. Jalan Ir. Sutami 36A Surakarta 57126, CentralJava, Indonesia. Tel./Fax. +62-271-663375, email:[email protected]; [email protected]

Cattle breeding is regarded indigenous to Indonesia. In thecountry, there are three species of cattle breeds, i.e.: zebu(Bos indicus), Bali cattle (Bos javanicus), and taurine (Bostaurus). These breeds are farmed for their meat, milk,hides, and for agricultural work. Zebu were introduced bythe Indians in the beginning of the first century. Bali cattleare indigenous breeds that have been domesticated fromwild bantengs (Bos javanicus) in Java and Bali forhundreds of years. Several breeds of taurine were importedin early eighteenth century to be used for dairy cattle. Zebuand taurine are the major cattle breeds of the world;whereas in Indonesia, the major cattle breeds are Balicattle, Ongole crossbred, and Madura cattle, which is acrossbred of the two. Primary breeding between species inthe genus Bos will result in sterile male and fertile femaleoffspring. However, secondary breeding with a crossbredfemale will result in fertile offspring. In Indonesia, thereare several local cattle breeds of zebu that have adapted tothe local condition, for example Ongole crossbred, Acehcattle, Pesisir cattle, Sumba Ongole, and the less commonlyfound Galekan cattle of Trenggalek. In addition, there aremany hybrids between zebu and Bali cattle such as Maduracattle, Jabres cattle of Brebes, Rancah cattle of Ciamis, andRambon cattle of Bondowoso, Banyuwangi, and thesurrounding areas. A crossbreeding between zebu andtaurine produces Grati dairy cattle. In 1970s, an ArtificialInsemination program was conducted in a large scale usingmale cattle and semen from several breeds of zebu(Brahman, Brahman Cross) and taurine (particularlySimenthal, Limousin, Holstein Friesians). The programresulted in more complex genetic mixes. Crossbreedingconducted directly in the field causes a concern since it


may threaten the purity of the native species and decreasethe cattle’s potential for adaptation, reproduction, andproductivity. It is better to conduct crossbreeding programsprivately in research centers or corporate/large farmers, ofwhich the result can be distributed to smaller farms.“Ongolization program” that was introduced in the earlytwentieth century should be a lesson to learn, because ithad led to the extinction of Javanese cattle, while theproduced offspring, the crossbred Ongole, are consideredunsatisfactory so that they still have to be crossbred withother species of cattle, particularly taurine.

Bali cattle, crossbreeding, local cattle, taurine, zebu

AP-02The performance of agronomic character anddiversity from 62 accession of sweet potatogermplasm

Wiwit Rahajeng♥, St. A. RahayuningsihIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Sweetpotato (Ipomoea batatas) is an alterativecarbohydrate source to overcome the shortage of rice dueto the impact of the higher population growth and climatechange. This study aims to determine the performance andthe range of genotypes and phenotypes of agronomiccharacters 62 germplasm accessions of sweetpotato. Theexperiment was conducted in April-August 2013 atMuneng experimental station, Probolinggo, East Java. Thematerial used was 62 accessions of sweet potato ILETRIgermplasm collection. The research was arranged in arandomized complete block design (RCBD) with tworeplications with plot size of 1 m x 5 m, with 100 x 25 cmspacing. Harvesting was done at 4 months after planting.Variables observed included: vines length, branch number,chlorophyll index, the weight of the canopy, harvest index,root length, root diameter, number of tuber perplot, tuberyield, and tuber dry matter content. Results of analysis ofvariance showed highly significant genotype at all thecharacters observed. MLG 12695 showed the highest tuberyields followed by MLG 12505, while the MLG 12707showed the lowest tuber yields followed by MLG 12645.All the characters show a wide range of phenotypes andgenotypes are broad. All the characters also showed highbroad sense heritability.

Agronomic characters, Ipomoea batatas, sweet potato,phenotype diversity genotype diversity

AP-03Genetic diversity of sun-flower (Helianthusannuus) germplasm based on quantitative andqualitative morphological characters

Rully Dyah Purwati1,♥,, Anik Herwati2

1Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI). Jl.Raya Karangploso 199, Malang 65152, East Java, Indonesia. Tel.: +62-341-491447, Fax.: +62-341-485121. ♥email: [email protected] Indonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl.Raya Kendalpayak Km 8, PO Box 66 Malang 65101, East Java,Indonesia.

In Indonesia, sun-flower has not been widely cultured byfamers and companies who produce sun-flower oil.Recently, many people are interested in to developed thiscrop due to their lots of benefits. Indonesian Sweetener andFibre Crops Research Institute (ISFCRI) have started toprepare some varieties for sun-flowers (Helianthus annuusL.) development in the future. Sun flower germplasmcollection in ISFCRI has been characterized.Characterization was aimed to distinguish themorphological characters of each genotype in germplasmcollection. Based on these morphological quantitative andqualitative characters, the genetic diversity of sun-flowergermplasm was observed. The activity was carried out inthe Pasirian Experimental Station, Lumajang District. Thelocation is placed on 110 m above sea level and in 113° Eand 8° LS. The climate in this location is included in Ctype (Smith Ferguson), with an annual rainfall of 1,700 mmand 120 rainy days per year. Research was conducted inMarch to December 2015, germplasm characterized were33 accessions. Each accession was planted in 32 m² plotsize with four lines. Fertilizer dose was 75 kg Nitrogen +30 kg P2O5 + 30 kg K2O per ha. The results showed thatthe low coefficient of variation values were found in somequantitative morphological characters of Sun-flower,namely: seed size (3.51%); 100 seeds weight (2.60%);seeds thickness (4.82%); plant height (9,62%); leaf size(8.85%); ray floret length (7.32%); bract length (12.06%);head size (6,87%); and the flowering time (4.37%). Whilethe qualitative characters observed in sun-flowergermplasm presented high coefficient of variation values,except pollen production character in flower discs. Theseresults indicated that sun-flower accessions showed a highgenetic diversity based on the qualitative morphologicalcharacters.

Characters, diversity, qualitative, quantitative, sun-flower

AP-04The effect of mutations induced by gamma-rayirradiation to the performance of Reutealistrisperma

Rully Dyah Purwati♥, Parnidi, Aprilia Ridawati, MalaMurianingrumIndonesian Sweetener and Fiber Crops Research Institute (ISFCRI). Jl.Raya Karangploso 199, Malang 65152, East Java, Indonesia. Tel.: +62-341-491447, Fax.: +62-341-485121. ♥email: [email protected]

Reutealis trisperma (Blanco) Airy Shaw is one of thepotential commodities as bio-fuels. Improvement of R.trisperma new varieties needs sufficient genetic materialsso the expected new varieties can be produced. To increase


genetic diversity of R. trisperma, the radiation mutation bygamma-ray was done.This study aimed to determine theeffect of mutations induced by gamma-ray irradiation to theperformance of R. trisperma. The treatment consists of 7doses of gamma ray irradiation are: 50, 100, 150, 200, 250,300 Gy and control. Experiment gamma irradiation (60Co)at 100 R. trisperma seed (KS 2) each doses, conducted atthe Center for Isotope and Radiation Applications(BATAN) Jakarta. Evaluation of germination was done byplanting seeds in poly-bag 10 x 12 cm with sterile sandmedium in Indonesian Sweetener and Fiber CropsResearch Institute. At the age of 30 days displacement inpoly-bag 25x25 cm with soil: sand: manure in the ratio 1:1: 1. Maintenance of seed was done up to the age of 4months. Based on the results of the evaluation showed thatgamma irradiation had positive effect on germination rateR. trisperma. Irradiation may increase between 5.28 to 9.26times faster than control. Lethal doses of gamma rayirradiation for R. trisperma is 150 gray. While theconcentrations of 250 and 300 Gy resulted in damage to R.trisperma. Beside that, Gamma ray irradiation increase thediversity of R. trisperma plant leaves. In general, thegrowths of mutants R. trisperma up to the age of 4 monthsimproved by radiation mutation compare to control.

Gamma-ray, mutation radiation, Reutealis trisperma

AP-05Response of 14 soybean genotypes to whiteflies(Bemisia tabaci): Antixenosis, antibiosis, andtolerance

Apri Sulistyo♥, Alfi InayatiIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

The attack of whiteflies (Bemisia tabaci Genn.) in soybeancultivation in Indonesia is one of the limiting factors inincreasing the national soybean production. Plantingresistant varieties could reduce yield losses due to thedamage caused by these pests. This study was conducted toevaluate the resistance of 14 soybean genotypes to thewhiteflies. A free-choice test and no-choice test wasconducted in a greenhouse to study the antixenosis andantibiosis. Meanwhile, field testing was conducted todetermine the tolerance of soybean genotypes to thewhiteflies. Determination of the resistance of soybeangenotypes to whiteflies based on the intensity of leafdamage that occurs on fifth weeks after infestation.Anjasmoro varieties which used as susceptible checksshow sensitivity to whiteflies. Adults and nymphs in smallnumbers on the surface of the leaves of Anjasmoro hasbeen causing damage to leaves of up to 76.59% in free-choice test and 45.15% in no-choice test.G100H/9305//IAC-100-271 demonstrates the sensitivity towhiteflies that is similar to Anjasmoro. Gema varieties thatserved as moderately resistant check indicate antixenosis

mechanism that correlated with leaf thickness, length anddensity of leaf trichomes. The characteristics of longtrichomes and rarely as well as thick leaves cause Gema arenot favored by the whiteflies for oviposition. There are fourgenotypes showed antixenosis mechanism similar to Gema,namely IAC-100/Kaba-6, Malabar/IAC-100-85, Kaba/IAC-100//Burangrang-60, and Kaba/IAC-100//Burangrang-63.In the no-choice test, antibiosis mechanism can be seenfrom the small number of adults that develop from nyhmps.IAC-100/Kaba-8 and IAC-100/Kaba-14 showed a highdegree of antibiosis. In addition, the results of fieldexperiment showed that Gema, IAC-100/Kaba-14, andTanggamus/Pangrango-78 demonstrated a tolerancemechanism to whiteflies. It is shown from a slightlydecrease in grain yield of the three genotypes (17.33,19.31, and 19.85% respectively).

Glycine max, host plant resistance, non-preference,resistance mechanism, yield reduction

AP-06The response of soybean genotypes introducedfrom South Korea to drought stress during thereproductive stage

Suhartina♥, Novita Nugrahaeni, Apri SulistyoIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Soybean productivity is still low. There is an urgent need touse more land to increase soybean production. Theobjective of this study was to evaluate the productivity andenvironmental adaptability of several soybean genotypeintroduced from South Korea to drought stress duringreproductive stage. The study conducted on twenty soybeangenotypes originated from South Korea and three varietiesas checks (Mutiara, Grobogan, and Dering 1). The entiregenetic material planted at the Kendalpayak ExperimentalStation and under rain-shelter in Malang on dry season in2012. Each genotype was planted in single rows along 2 mand without replication. Row spacing used was 40 cm x 15cm. Fertilization was conducted according to the dosagerecommendation i.e. 100 kg ha-1 of Urea, 100 kg ha-1 ofSP36, and 75 kg ha-1 of KCl. Irrigation was only given atplanting and during flowering for drought stress conditionduring the reproductive phase. Observations included daysto flowering, days to maturity, plant height, number ofbranches, number of fertile nodes, number of pods, numberof empty pods, weight of 100 seeds, and weight of seedsper plantThe results showed that five of 20genotypes weretested had resistance to drought stress at the reproductivephase, equivalent to Dering 1. Four genotypes of them(Daewon, Ilmi, Jangmi, and Mausu) has a large seed sizeand early maturity.

Drought stress, Glycine max, genotype introduce,reproductive phase


AP-07Improvement of genetic variability in seedlings ofSpathoglottis plicata orchid through X-rayirradiation

Suyitno Aloysius1,2,♥, Aziz Purwantoro3, Kumala Dewi1,Endang Semiarti1,♥♥

1Graduate Study Program of Biology, Faculty of Biology, Gadjah MadaUniversity. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta,Indonesia.Tel/Fax. +62-274-580839, ♥email: [email protected],♥♥[email protected] of Biology, State University of Yogyakarta, Karangmalang,Yogyakarta, Indonesia3Faculty of Agriculture, Gadjah Mada University. Jl. Flora Bulaksumur,Sleman 55281, Yogyakarta, Indonesia

Developing genetic variability of orchids via mutation ispromising for orchid breeding. The objective of thisresearch is to improve genetic variation of Spathoglottisplicata orchids through X-ray-irradiation of the orchidseeds. The method was conducted using seedlings that X-ray irradiated with various doses (0, 6, 12, 18, and 24 rad).The seeds were sowed on a half strength of MS medium,seeds were grown into protocorms (tuber-like developingorchid embryo), 8 weeks-old were subcultured onto NP-SIM medium. Five months-old seedlings then subculturedagain into a new flask. The observation is focused on themorphological variation of the seedling. Genetic variabilitydetection is conducted using PCR RAPD based on nineprimers, those were OPA1, OPA2, OPA11, OPA12,OPA14, OPB1, OPB4, OPD12, OPD14. The data isanalyzed using GenAlex 6.1 software to obtain the geneticdistance. Moreover, utilizing NTSys ver.2, the data wasanalyzed for clustering by means of UPGMA method and adendrogram based on Neighbour Joining model iscompleted. The result showed that X-ray irradiation withdoses of 18-24 rad is able to stimulate morphologicalvariation of seedlings, especially characters of leaf, rootand shoot. Interestingly, X-ray irradiation was also inducedin vitro early flowering. Based on the dendrogram ofgenetic distance and polymorphism percentage of the groupof mutants which are bigger or farther from wild typegroup, it is concluded that 18-24 rad doses of X-rayirradiation can be used for induction of genetic variabilityin orchid.

Genetic variability, seed, seedling phenotype, Spathoglottisplicata, X-ray

AP-08The study of soybean genotipes response againtsAsian soybean rust (ASR)

Alfi Inayati♥, Eriyanto YusnawanIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Asian soybean rust (ASR) caused by Phakopsorapachyrhizi is a major disease limiting soybean yield andhas widely spread on soybean plantation in Indoesia. Theresistant cultivars availability is one of key component forthe successful of ASR control. The objectives of this studywere to assess the resistance of soybean lines from thecrossing of two large-seeded cultivars (Baluran andGrobogan) with broad adaptation cultivar (Kaba), andidentify the characteristic of resistant genotypes underscreen house conditions. All genotypes tested wereartificially inoculated with P. pachyrhizi at three weeksafter planting. The observations were made on the numberof pustules per leaf, the development of ASR trough themodified IWGSR method, and yield components includingnumber of intact pods per plant, number of empty pods,and weight of pods per plant. The result showed thatthirteen lines of Baluran pedigrees have higher resistanceresponse to ASR compare with Grobogan pedigrees.Resistant lines have fewer number of pustules (8 pustulescm-2), lower value of area under the disease progress curve(AUDPC), and have redish brown (RB) lesion type. Incontrast, susceptible lines have plenty of pustules (> 21pustules cm-2, higher AUDPC value, and has mixed lesiontype (RB and Tan). ASR reduces seed size and yield. Theaverage weight of 100 seed on resistance lines was 10.2grams while on susceptible lines ranged from 8.74 to 12.64grams. The average yield per plant varies from 2.79 gramto 6.11 grams. Baluran/Grobogan pedigrees moresusceptible to ASR than Baluran/Kaba pedigrees but theyshowed better yield per plant, and supposedly more tolerantto ASR.

Asian soybean rust, genotype, resistant, soybean, susceptible

AP-09Genetic relationship among Indonesian mungbeangermplasm based on quantitative traits

Ratri Tri Hapsari♥, Trustinah, Rudi IswantoIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Mungbean (Vigna radiata) is an important cash croplegume in Indonesia, especially in dryland. Identificationand evaluation germplasm collections plays important rolefor mungbean breeding program. The aims of this studywere to determine the genetic relationship amongmungbean accessions based on quantitaive traits. A total of122 accessions of mungbean germplasm from localcollections Indonesia cultivated in Jambegede ResearchStation (Malang) on dry season 2014. Each genotype wasplanted in a single row along 4 m plot with plant spacing of50 x 10 cm, and two plant was maintained in every hole.Thirteen variables quantitative and five variable qualitativewere observed. Data were analysis using principalcomponent analysis (PCA) and cluster analysis. The resultsshowed that five principal components (PC) contributed


76% of total variation. The most important characters forPC 1 was number of branches, number of fertile branches,number of pod cluster, and number of filled pod, PC 2 wasdays 50% to flowering and maturing days, PC 3 was wiltdisease, PC 4 was seed weight per plant and plant height,and PC 5 was 100-seed weight. The dendogram clustered122 accessions into four groups. Based on clusters analysisthere were four clusters with similarity distance 72.29%.Cluster I was the largest group which consisted of 59accessions. Cluster II consisted of 45 accessions. Cluster IIIconsisted of 11 accessions and Cluster IV consisted ofseven accessions. There was no parallelism betweengeographical distributions in each cluster. Among them,cluster IV was the most interesting, as its member consistof accessions which susceptible to wilt disease.

Genetic diversity, Vigna radiata, wilt disease

AP-10Phenotypic test and identification of microsatellitemarkers related to aluminium tolerance in uplandrice

Yuliana Galih Dyan Anggraheni♥, Sri Indrayani, EnungSri MulyaningsihResearch Center for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected]

Upland rice grown in many sub optimal fields such ultisolfield that spread in Indonesia. The main obstacle ofutilizing ultisol field is the high acidity levels, whichcaused by aluminum (Al) stress. In order to utilize thatland, we require Al tolerant upland rice varieties.Identification of upland rice coding Al tolerant trait has notbeen much studied yet. The research objectives are to studyand identify markers for upland rice coding regarding to Altolerance. The genetic material in this study used 36 ricelines F6 generations from B11930F-TB-2 x SituPatenggang. The strains were selected on acid field in EastLampung by category: tolerant, moderate and sensitive toAl. ITA and IR60080-32 varieties was used as acomparative control for sensitive and tolerant. Phenotypictest for Al tolerance in laboratory used nutrient culture with60 ppm AlCl3. While genotypic test was done withselection of SSR markers that suspected linked to Altolerant. Phenotypic test result showed a shift in the levelof tolerance between the results in the field (EastLampung) and result in nutrient culture (lab). Suspectedfrom the results on field, other than specified by thedissolved Al content, it was also influenced by other factorssuch as microclimate and soil fertility diversity, while innutrient culture results showed that stress occurred as asingle factor because of dissolved Al. The markers analysisresults showed that markers RM257, RM340, RM481,RM201, RM526 and RM17 are considered as the mostinformative markers to use as coding for Al tolerantbecause it has PIC (Polymorphic Information Content)

amount as 0.5. Phylogenetic tree shows three groups ofupland rice: (i) ITA, (ii) IR6008032 and B11930F-TB-2,(iii) Situ Patenggang and crossbred strains.

Aluminum tolerant, SSR markers, upland rice

Diversity of species

BO-01The impact of deforestation rate for extinctionbiodiversity in Indonesia

I Putu Gede ArdhanaDepartment of Biology, Faculty of Mathematics and Natural Sciences,Udayana University. Jl. Kampus Bukit Jimbaran Denpasar, Bali Tel./Fax.+62-361-703137, 701954 ext 226, ♥email: [email protected]

The purpose of this study was to examine the impact ofdeforestation on biodiversity loss in Indonesia. Firstlyauthor presented information about deforestation trendsthat spread across Indonesia. And presented informationabout forest fires that triggered off the continuousdeforestation and occurred one after another throughout theyear. The collected data showed deforestation and forestfires have occurred since 1960 to 2015, and deforestationand forest fires implicated in the extinction of speciesdiversity, genetics, and ecosystems that spreads fromSunda region to Sahul region. Author used descriptiveregulation and legislation methods, used literatureapproach, as well as arranged with descriptive andinterpretational form in papers. From the results of thisstudy author concluded that deforestation rate implicates inforest fires that occur continuously throughout the year andcan not be inevitable possibility of extinction ofbiodiversity spread across Indonesia.

Deforestation, impact, forest fires, biodiversity loss

BO-02Lichen: Comparative study between Campus Areaof Universitas Muhammadiyah Surakarta andmountain forest of Sekipan Karanganyar, CentralJava

Efri RoziatyDepartment of Biological Education, Faculty of Teacher Training andEducation, Universitas Muhammadiyah Surakarta. Jl. A Yani Tromol Pos1 Pabelan Kartasura, Sukoharjo 57169, Central Java, Indonesia. Tel.: +62-271-717417 Ext. 147, Fax: 0271-715448. email: [email protected]

Lichen is a plant that lives attached to the trunks of trees,rocks. Lichen is very sensitive to pollutants. The rapidincrease in the use of motor vehicles have a large impact on


the emergence of pollution in the region. Increasing thequantity emitted by the transport sector, especially vehicleson the road resulting in high levels of air pollutioninfluenced the morphology of lichen. The research locationis divided into two kinds of wide area i.e. campus area andthe forest. The research using exploration method. The 1ststation was the campus area and the 2nd station was in theforest. The parameters were lichen morphology and lichencolonies. Species richness was locally in the forest, withsomewhat higher diversity of lichens and lower diversity oflichen in the campus area. The results showed that theenvironment is directly affected by the activities ofpollutants come from motor vehicles emission can be seenfrom the percentage of lichen that grows in the area.

Air pollution, pollutants, bioindicator, lichens, emissions

BO-03Preliminary test of Agri-Environmental Schemeadoption in farmland of northern slope of MountSlamet, Central Java, Indonesia

Imam Widhiono♥, Eming Sudiana, Edy Tri SuciantoFaculty of Biology, Jenderal Soedirman University. Jl. Dr. Soeparno No.68, Purwokerto, Banyumas 53122, Central Java, Indonesia. Tel. +62-281-638794, Fax: +62-281-631700, ♥email: [email protected]

Agricultural intensification poses the largest threat tobiodiversity that affect ecosystem services such aspollination and considerable result showed that pollinatordecline may have economic consequences. Agri-Environmental Scheme (AES) were introduced in Europein the early 1990s in response to declining farmlandbiodiversity. The provision of floral resources for theenhancement of insect pollinators populations inagroecosystem as one of AES methods has been done atNorthern Slope of Mount Slamet at May to October 2012.The three of main crops (tomato,strawberry and chilipeppers) combined with 0%,5%,10%,and 15% of wildplant species i.e Cleome rutidosperma, Tridax procumber,Boreria laevicaulis and Euporbia heterophylla. The resultshowed that species richness of insect pollinators hasdifferent between wild plant C. rutidospremae (8.35species), B. laevicaulis (8.17 species), E. heterophyla (8.89species), and T. procumber (8,39 species) and block oftomatos showed the highly species richness (11.5 ± 0.57).Enhancment of agroecosystem with four wild plant anddifferent density showed that combination with 15% hashighest species richness of insect pollinators. From theresult can be concluded that enhancement ofagroecosystem with wild plants species increase insectpollinators species richness

Agri-Environmental Scheme, diversity, enhancment, insectpollinators, wild plant

BO-04Bioaccumulation of copper by microorganismsisolated from industrial sewage in Rungkut-Surabaya, Indonesia

Wahyu Irawati1,♥, Salomo Christian1, TriwibowoYuwono2

1Universitas Pelita Harapan. M.H. Thamrin Boulevard 1100, LippoKarawaci, Tangerang 15811, Banten, Indonesia. ♥email:[email protected] of Microbiology, Faculty of Agriculture, Gadjah MadaUniversity,Jl. Flora Bulaksumur, Sleman 55281, Yogyakarta, Indonesia

Heavy metal pollution is a serious problem mainly causedby industrialization, that produces large quantities ofwastewaters containing high concentrations of heavymetals. Wastewater consists of microbial populationsadapted to the toxic concentrations of heavy metals andbecome resistant by accumulating copper inside the cells.The aim of the study was to characterize the ability ofmicrobial isolates isolated from industrial sewage inRungkut-Surabaya in accumulating copper. The copperresistance of microbial isolates were determined bymeasuring minimum inhibitory concentration (MIC). Theability of each isolates to accumulate copper weredetermined by atomic absorbtion spectrophotometer. Theresults showed that there were eight bacterial isolates andnine yeast isolates with the MICs of 6-7 mM, and 16-20mM CuSO4, respectively. Some of bacterial isolates wereGram negative bacteria. Three highly copper resistantbacterial isolates and two highly resistant yeast isolateswere designated as isolates C1, C2, C4, and isolates ES9.3,ES10.2, respectively. Isolates C1, C2, and C4 accumulatedcopper up to 292,93 mg, 508,01 mg, dan 371,42 mg Cu pergram dry weight of cells, respectively, meanwhile isolatesES9.3 accumulated Cu of up to 0.52 mg per gram dryweight of cells. Copper resistant microorganisms with theirability to accumulate copper may have significant role inwastewater treatment plant.

Bioaccumulation, copper-resistance, bacteria, yeast

BO-05Diversity of parasitic plants and their hosts inhomegardens agroforestry in Kepala Jeri andPemping Islang, Batam

SolikinPurwodadi Botanical Garden, Indonesian Institute of Sciences (LIPI). Jl.Raya Surabaya-Malang Km 65, Pasuruan 67163, East Java, Indonesia.Tel. +62-343-615033, Fax. +62-343-615033, email: [email protected];[email protected]

Parasitic plant grow attached to host plants either cultivatedor wild plants as parasite either cultivated or grow wild.Research aimed to know diversity of the parasitic plantsand their hosts in home gardens agroforestry wasconducted in Kepala Jeri and Pemping Islands Batam inOctober 2014 using explorative methods. Inventory and


data collection were done along determined tracks ofexploration. There were three species of the parasitic plantswere found namely Cassytha filiformis L., Dendrophthoepauciflora Dans. and Viscum stenocarpum Dans. The hostplants of parasitic plants were 26 species, 24 genera and 17families. The most dominant parasitic plants was Cassythafiliformis L. whereas least dominant was Viscumstenocarpum Dans. The host plants of Dendrophthoepauciflora Dans. were dominated by Parkia speciosaHassk., Mangifera indica L. and Artocarpus integer(Thunb.) Merr. whereas the host plants of Cassythafiliformis L were dominated by Melastoma malabathricumL. dan Adinandra dumosa Jack.

Agroforestry, parasitic plant, host

BO-06The diversity and distribution of Holothuroidea inshallow waters of Baluran National Park,Indonesia

Arif Mohammad Siddiq♥, Tri Atmowidi, Ibnul QayimDepartment of Biology, Faculty of Mathematics and Natural Sciences,Bogor Agricultural University. Jl. Raya Darmaga Bogor 16680, WestJava, Indonesia. Tel./Fax. +62-251-8622833 ♥email:[email protected]

A study of the diversity and distribution of sea cucumber(Holothuroidea) in shallow waters at Baluran NationalPark, East Java, Indonesia was carried out from July untilSeptember 2015. The method used in this study wassystematic transect in low tide condition. Samples werecollected by hands at intertidal sites. Identification of seacucumber species based on morphological ossicles. Twentyone species of Holothuroidea belonging two orders andfour families were found in this study. The most dominantfamily found was Holothuriidae (16 species), followed byStichopodidae (2 species), Synaptidae (2 species), andChiridotidae (1 spesies). Four species (Holothuria olivacea,H. verrucosa, Labidodemas rugosum, and Chiridotasmirnovi) are new record for Java waters and one species(H. papillifera) is a new record for Indonesian waters. Twomorphospecies (H. aff. macroperona and Stichopus cf.monotuberculatus) need reconfirmation to species level.The highest abundance species of Holothuroidea was foundat under rock with 15 species. Whereas, the highest numberof individuals was found in seagrass areas with 5457individuals. H. atra has extensive habitat distribution, suchas seagrass, macroalgae, coral reef, dead coral, sand, andunder rock.

Baluran National Park, distribution, diversity,Holothuroidea, shallow waters

BO-07Methanolic extracts of Ageratum conyzoides,Amaranthus spinosus and Cyperus rotundus asbotanical fungicides to control peanut rust disease

Eriyanto Yusnawan♥, Alfi InayatiIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Rust disease caused by Puccinia arachidis is one of themost important diseases on peanuts. Ageratum (Ageratumconyzoides L.), spiny amaranth (Amaranthus spinosus L.),and coco-grass (Cyperus rotundus L.) had the ability toinhibit plant pathogens. The aim of the research was toobtain the weed extracts effective to control peanut rustdisease. The three weeds were ground to obtain fineparticles, macerated in methanol for 18 h and evaporatedthe solvent using vacuum rotary evaporator. Methanoliccrude extracts obtained from ageratum, coco-grass, andspiny amaranth at concentrations of 0.1%, 1.0%, 2.5%, and5.0% were applied to the urediospores and peanut plantsinfected with the pathogen. Applications of 5% ageratumand 5% coco-grass extracts suppressed the sporegerminations of 78 to 80% and 76 to 80%, respectively.Disease intensities on Kancil cultivar treated with 5% crudeextract of ageratum were 18 to 22% and 29 to 31% afterthree and four times of extract applications. Diseaseintensity on untreated plants were 30 to 32% and 43 to46%, whereas the chemical application suppressed thedisease intensities up to 16 to 18% and 15 to 17% at thesame observation. Preliminary phytochemical screeningsshowed that the methanolic extract of ageratum containedalkaloids, flavonoids, tanins, saponins, and terpenoids.Crude extract of 5% ageratum was effective to control rustdisease on peanuts, therefore, could be used an alternativecontrol to suppress the disease.

A. conyzoides, A. spinosus, botanical fungicide, C. rotundus,peanut rust disease

BO-08Pattern of insect community associated withSantalum album

Arina Damayanti♥, Ananto Tiyogo, Yeni Widyana NRDepartment of Silviculture, Faculty of Forestry, Gadjah Mada University.Jl. Agro No. 1, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia,♥email: [email protected]

Plants play an important role in the trophic levels. As aprimary producer at the basal level, plants are in turnconsumed by the organisms on the second trophic level,herbivores, as a primary consumer. This research wasconducted using Santalum album Linn. which growsnaturally in Nglanggeran, Gunungkidul. This research wasconducted to determine the insect community in S. albumand to determine the effect of variations of characteristics


of S. album flower. The research was conducted at the peakflower period in February 2015. The observation time wasdivided into three different levels: (i) anthesis, (ii) anthesisto late anthesis, and (iii) late anthesis. The insectcommunity observation was conducted by direct samplingmethod, then all of insects found will be recorded.Identification of insect held at the Laboratory ofEntomology, Faculty of Agriculture, University of GadjahMada, Yogyakarta. The influence of flowering phase toinsect community was measured by ANOVA statisticalanalysis. Insect communities in S. album consist of 7 ordersand 23 families, consisted of the second trophic level:Hymenoptera (consists of 7 families); Diptera (2 families);Hemiptera (3 families); Lepidoptera (5 families);Coleoptera (2 families); and Orthoptera (1 families), andthe third trophic level: Hymenoptera (5 families); Diptera(2 families); Coleoptera (2 families); and Odonata (1families). Some insects were included into two trophiclevels: members of the order Hymenoptera and Diptera.Flower abundance was significantly influenced FamilyFormicidae, Vespidae, Eumenidae, Syrphidae, Muscidae,Hesperiidae, Nymphalidae; while stratum affected theVespidae, Eumenidae, Scoliidae, Hesperiidae, Pieridae,Nymphalidae.

Insect community, effect of flowering, trophic levels,Santalum album

BO-09Natural regeneration of burnt peat swamp forestand burnt peat land in core area Giam Siak KecilBukit Batu Biosphere Reserve Riau Province andIts implication on conservation

Elfis♥, Prima Wahyu Titisari♥♥

Departement of Biology, Faculty of Teacher Training and Education, RiauIslamic University. Jl. Kaharuddin Nasution No. 113 MarpoyanPekanbaru. Tel.: +62-761-674674. ♥email: [email protected], ♥♥

[email protected]

A designated forest with specific purpose in core area ofGiam Siak Kecil, Bukit Batu Biosphere Reserves, is one ofpeat swamp forest areas where multiple fire occured. Thisresearch aimed to analyze composition and diversity ofvegetation, regeneration and similarity among different firehistory of peatswamp forests, e.g. secondary peat swampforest (HS), burnt peat swamp forest in 2000 (HT2000),multiple fire of peat swamp forest (HT2005, HT2010,HT2014) and agroforestry on burnt peatland (AF). HT2000and HT2005 have different vegetation compositioncompared with HS. Calophyllum macrocarpum was thecommonest tree species in HS, whilst Shorea was thecommonest tree species in HT2000, HT2005, HT2010 andHT2014. Similarity index of Sorensen (IS) between HS andHT2000, HT2005, HT2010, HT2014 was relatively high(IS = 68.43%), meanwhile Sorensen index between HS andHT2000, HT2005, HT2010, HT2014 was considerably low(IS = 33.12%). Fire reduced tree diversity, which wasindicated by low Shannon-Wiener diversity index (H’). H’

index in HS was 3.41, whereas H’ index in HT2000 andHT2014 were 2.89 and 1.63, respectively. There were 33tree species found in forest only; and 68.43% of the specieshas zoochory dispersal mode. Dispersal mode of thespecies in different habitat need to be taken into account forconservation practices in core area of Giam Siak Kecil,Bukit Batu Biosphere Reserve Riau Province.

Seed dispersal, species composition, zoochory

BO-10Mangrove succession in the restoration areas ofSembilang National Park, South Sumatra

Sarno♥, Harmida, Nita Aminasih, Hanifa Marisa andRanti SeprinaBiology Department, Faculty of Mathematics and Natural Sciences,Sriwijaya University. Jl. Palembang-Prabumulih Km 32, Indralaya, OganIlir 30662, South Sumatra, Indonesia. ♥email:[email protected]

Mangrove conditions in the region under pressure anddegradation from year to year. The main cause ofmangrove destruction in the Sembilang National Park(SNP) is cultivation or manufacture of fish ponds,especially in the Banyuasin Peninsula, South Sumatra. Theactivities of these ponds have resulted in the degradation ofmangrove, especially in greenbelt. The destruction ofmangrove areas causes a decrease in the quality and extentof mangrove areas which result in the degradation of avariety of important functions. Damage to mangroves occurboth at the regional, national and even up to the globallevel. The purpose of this research is to know the processof natural mangrove revegetation on former ponds. Thecomposition of the type of vegetation that grows in the areaof the former ponds in South Sumatra region SNPrestoration consists of four species: Avicennia marina,Avicennia alba, Rhizophora mucronata, and Portulacavillosa. The most dominant species of mangroverevegetation of former pond is Avicennia marina.

Former pond, mangrove, restoration, succession, SembilangNational Park

BO-11Assosiations of epiphytic microalgae onmacroalgae with macroalgae thallus form inMuara Binuangeun Reef Flat, Lebak, Banten

Aprilian Pryski Waskitho Adi, Titi Soedjiarti, RianiWidiartiDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl. Lingkar KampusRaya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163, Fax.:+62-21-78849010. email: [email protected]


Research on the assosiation of epiphytic microalgae withmacroalgae thallus form have been conducted on April30th-May 3rd 2015 in Muara Binuangeun, Lebak, Banten.The research purposed are to determined the abundance ofepiphytic microalgae on macroalgae, and to analyzed theassosiation of epiphytic microalgae with macroalgae formin Muara Binuangen. Samples of epiphytic microalgaewere taken from 4 stations, selected based on macroalgaepresence. Epiphytic microalgae which attached tomacroalgae surface were separated using shaking method.The water was then filtered twice, using sieves of 125 µmand 20 µm. Microalgae on 20 µm sieve were observedusing light microscope. The associations between epiphyticmicroalgae and macroalgae form was tested using ChiSquare test. Based on the results, Navicula was the mostabundant epiphytic microalgae in Muara Binuangeun. Thehighest abundance of epiphytic microalgae were found infleshy macroalgae with rough surface, which is Sargassum.Epiphytic microalgae that has associations with macroalgaeform is Amphora, which has positively associated withfoliose thallus form.

Association, epiphytic microalga, macroalgae thallus form,Muara Binuangeun

BO-12The aviability of feed plants resources of JalakLawu (Turdus poliocephalus stresemanni Bartels.)in Mount Lawu

Muhammad Ridwan1,, Suratman2, Sugiyarto2

1 Biodiversity Study Club (KS BIODIV), Department of Biology, Facultyof Mathematics and Natural Sciences, Sebelas Maret University. Jalan Ir.Sutami 36A Surakarta 57126, Central Java, Indonesia. Tel./Fax. +62-271-663375, email: [email protected] Department of Biology, Faculty of Mathematics and Natural Sciences,Sebelas Maret University. Jl. Ir. Sutami 36A Surakarta 57 126, CentralJava, Indonesia.Jalak Lawu or jalak gading (Turdus poliocephalusstresemanni Bartels.) is an iconic bird in Mount Lawu andreported that its distribution is limited to this mountainarea. The bird population is likely to decline due to habitatdestruction such as annual forest fire and poaching. Thisstudy aimed to determine the aviability of its feed plantsresources and bird abundance throughout differentelevation in mount Lawu as early stage of conservationeffort. Square method and point count with seven stationsbased on altitude, namely 2.000, 2.2000, 2.400, 2600,2.800, 3.000 and 3.200 m asl, were employed to analyzedistribution and abundance of feed plants and to measurethe bird abundance. Data of feed plant were analyzeddescriptively qualitatively. The results suggest that JalakLawu consume edible part (ripe fleshy fruits) of 9 obtainedfeed plants species of Jalak Lawu scilicet Polygonumchinense (herb), 5 species of shrubs Rubus fraxinifolius,Rubus linaetus, Rubus niveus, Rubus chrysophyllus andDebregesia longifolia, and 3 species of trees includesVaccinium varingiaefolium, Photinia integrifolia, andMyrsine avenis. All species of feed plant has clustered

distribution pattern and each distributed at intervals ofdifferent elevation. Only Polygonum chinense aredistributed in widest area (2.000-3.200 m asl) and has thehighest individual abundance. Elevation of 2.200 m asl hashighet diversity of feed resources with 6 species of plantsbut bird abundance found most intens at 2.600 m asl whichhas lower feed plants diversity (5 species). Jalak Lawu islikely to move in habitat with abundant food resources, butthe activity is also influenced by competitor species.

Diversity, feed plants, Jalak Lawu, Mount Lawu

BO-13Plants diversity of the deforested peat swampforest of Tripa, Indonesia

Djufri, Wardiah, Z.A. Muchlisin1 Department of Biology, Faculty of Teacher Training and Education,Syiah Kuala University, Banda Aceh 23111, Aceh, Indonesia. email:[email protected] of Marine and Fisheries, Syiah Kuala University, Banda Aceh23111, Aceh, Indonesia

The purpose of the present study was to evaluate the plantsdiversity of the Tripa peat swamp forest in Aceh BaratDistrict, Indonesia. A quadratic method was employed inthis study. Determination of the square area was carried outbased on the curve of minimum area. The result showedthat there were 41 species of herbs with diversity indexranging from 1.8785 to 2.4180 classified as low tomoderate categories. A total of seven species of shrubs and24 species of trees were found at the locations withdiversity index (H') ranging from 1.5186 to 1.7496 and2.1713 to 2.9133 respectively, indicating the diversity ofshrubs was in the low category, while the diversity of tresswas in the moderate level. It is concluded that the diversityindex of herbs and shrubs were in the low category, whilethe tree groups was in the medium category. According tothe diversity index and direct observation of the Tripa peatswamp forest, this area has been degraded due to landconversion.

Deforestation, flora, peat swamp forest

BO-14Total phenolic, flavonoid content and antioxidantactivity of cultivated tabat barito (Ficus deltoidea)on various level on age

Hetty Manurung1,, Wawan Kustiawan2, Irawan W.Kusuma2, Marjenah2

1Department of Biology, Faculty of Mathematics and Natural Sciences,Mulawarman University. Jl. Barong Tongkok Kampus Gunung Kelua,East Kalimantan, Indonesia. email: [email protected] of Forest Products Technology, Faculty of Forestry,Mulawarman University. Jl. Ki Hajar Dewantara, Kampus Gunung Kelua,Samarinda East Kalimantan, Indonesia.


The aim of this research was to evaluate the total phenolic(TPC), flavonoid contents (TFC) and antioxidant activityleaf extract tabat barito (Ficus deltoidea Jack.) thatcultivated in glass house and in the field (Fd) on variouslevel on age (6, 9 and 12 months). Different water fieldcapacity on planting media in Glass house: W1 (100%),W2 (80%), W3 (60%) and W4 (40%) respectively used toestimate the TPC, TFC and antioxidant activity. Totalphenolic content was estimated using Folin-Ciocalteau’sreagent, total flavonoid content by aluminium chloridecolorimetric method and the antioxidant activity by theDPPH (2,2-diphenyl-1-picryl hydrazyl) method. Thehighest TPC (66.667±0.001 μg gallic acid equivalents(GAE)/mg extract) of leaf extract found on W3 at 12 monthold and the lowest (12.593±0.002 μg GAE/mg found onW1 at 6 months old. F.deltoidea that cultivated in glasshouse (W4) and in the Field (Fd) have the highest TFC382.051±0.008 and 371.795±0.003 μg catechin equivalents(CE)/mg extract respectively. Based on the DPPH assay,the IC50 Fd (31.763±0.049 μg/mL), W1 (57.556±0.047μg/mL), W2 (48.828±0.055 μg/mL), W3 (58.473±0.065μg/mL) and W4 (34.473±0.066 μg/mL) showed that theleaf extract F. deltoidea have strong antioxidant activity at12 months old as compare to 6 and 9 months old.

Antioxidant, cultivated Ficus deltoidea, flavonoid, phenolic

BO-15The density, composition, and mangrove foresthabitats in coastal areas of Torosiaje Jaya Villageof Popayato Sub-district, Gorontalo Province

Sukirman Rahim1,, Dewi Wahyuni K. Baderan2,,Marini Susanti Hamidun2,

1 PGSD Department, Faculty of Education, State University of Gorontalo.Jl. Jendral Sudirman 06, Kota Gorontalo, Gorontalo,Indonesia. email:[email protected] Department Biology, Faculty of Mathematics and Natural Sciences, StateUniversity of Gorontalo,Jl. Jendral Sudirman No. 6 Kota Gorontalo, Gorontalo Province,Indonesia, Tel. +62- 435-821125, Fax. +62-435-821752. email:[email protected], [email protected]

The ecosystem of mangrove is a quite good ecosystemwhich is located in Toroseaje village of Popayato Sub-district, Pohuwato District of Gorontalo Province. Thisbecause of the beach in the coastal of Toresiaje village is agently sloping beach. Further, this beach has depositedsediment and it is formed a promontory grave that causesthat mangrove in that region grows large and relativelyfertile. In addition, the mangrove which is located inPohuwato has fairly high various species. One of them isfound from Avicenniaceae family namely Avicenniamarina (Forsk.) Vierh. This study aims to (i) obtain theinformation about the density of the mangrove; (ii) todetermine the composition of mangrove species in coastalareas of Toroseaje Jaya village; and (iii) to know thehabitat of the species which is found in coastal areasToroseaje Jaya village. Besides, the data were collected by

purposive sampling. Moreover, for the measurement ofdensity, distribution type, diameter trees, and mangrovevegetation height use a distance method (Point-CenteredQuarter Method). Further, the composition types of viewsis based on the number species are found, and to obtain thedata of the habitat conditions of the species which hasdiscovered is using a direct observation in the field by atree and laboratory test sample originating from soilsamples in the study sites. Moreover, the result of thisstudy finds the four species of tree which dominate themangrove in Toresiaje Jaya village. They are Bruguieragymnorrhiza, Rhizophora mucronata, Rhizophoraapiculata, and Rhizophora stylosa with a density value of51.55 trees/3 ha with an average distance of 581.94 m/tree.Bruguiera gymnorrhiza and Rhizophora mucronata arespecies that dominate in the region due to supply mud assuitable habitat with its growth, besides it is affected by thesubstrate of mangroves in the village Torosiaje Jaya it isalso affected by salinity and temperature. Further, the datawhich have obtained, they can be used in a management ofmangrove forest which located in the coastal of Toresiajeand they can also be data in mangrove conservation effortsin order to reduce the effects of global warming.

Composition, density, habitat, mangrove forests

BO-16Effect of age differences in the presence of dungbeetles

Etik Susanti1,, Ardita Tri Anugrah Budaya1, DianSartika1, R.C. Hidayat Soesilohadi2

1 Entomology Study Group, Faculty of Biology, Gadjah Mada University.Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta,Indonesia.Tel/Fax. +62-274-580839, ♥email: [email protected] Faculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia

Dung is a necessity for the survival of the dung beetles.However, only the dung with certain characteristics that arefavored by the dung beetles. This study aims to determinethe dung with what age the most widely visited by the dungbeetles. The study was conducted in laboratory usingmethods olfactometry with five repetition and used threekinds of traps are respectively fitted with the treatment ofcow dung bait dung is still fresh, age a day, and more thanone days. Data analyzed by Completly Randomized Design(CRD). The results obtained that 33,14713% dung beetleschoose few days old dung, 38,53619% choose fresh dung,and 31,45532% choose one day old dung. It can beconcluded that the dung of the most widely visited by thedung beetle was the fresh ones.

Difference age of dung, dung beetles, olfactometry


BO-17Macroinvertebrate diversity in Water QualityAssessment of Winongo and Gajah Wong Rivers,Yogyakarta Special Region

Amelia Nugrahanigrum♥, Martina Faika Harianja,Hendriawan Nugroho, R.C. Hidayat SoesilohadiFaculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.Tel/Fax. +62-274-580839,♥email: [email protected]

Winongo and Gajah Wong are primary rivers inYogyakarta Special Region, Indonesia. Both rivers haveimportant role for society and surrounding areas. Thus,periodical river monitoring is needed. One way to monitorriver is by utilizing macroinvertebarate diversity. Thisresearch aimed to study macroinvertebrate diversity and toanalyze the water quality of Winongo and Gajah Wongrivers. Data was collected at upstream, middle, anddownstream zones of both rivers, 100 m each, by usingkick-sampling method. Diversity and abundance ofmacroinvertebrates was analyzed. The results showed thatthe number of macroinvertebrate families in Winongo was24 while in Gajah Wong was 26. The highest diversity wasin the upstream of Winongo river with diversity indexscore 2.411 while the lowest one was in the middle zone ofGajah Wong river with diversity index score 1.205. Bothscores of Family Biotic Index (FBI) and Biotilik Index ofWinongo river upstream was the highest with scores 4.64and 2.89, respectively. The lowest score of FBI was inWinongo river middle zone with score 8.19 while thelowest score of Biotilik Index was in Gajah Wong rivermiddle zone with score 1.29. From this research, it couldbe concluded that Gajah Wong river had highermacroinvertebrate family diversity than Winongo river andit could be implied that Winongo river upstream had thebest water quality while Gajah Wong river middle zone hadthe worst.

Diversity, Gajah Wong, macroinvertebrates, water quality,Winongo

BO-18Diversity of morphological and physiolyendophytic entomopathogenic fungi from peanutplant

Novri Nelly♥, Trizelia, ReflinaldonPlant Protection Department, Faculty of Agriculture, Andalas University.Kampus Unand Limau Manih, Padang 25163, West Sumatra, Indonesia.Tel. +62-751-72773, Fax.: +62-751-72702, ♥email:[email protected]

Endophitic fungi from peanut plant can be asentomophatogenenic. The purpose of this study was todetermine morphological and physiology the endophyticentomophatogenic fungi associated with peanut plant. Theresearch was carried out in the Biological Control

Laboratory, Faculty of Agriculture Andalas university fromMay to November 2015. Peanut plant samples were takenfrom Tanah Datar Sumatra Barat. This study used theexploration method by isolating endophitic fungus from theleaves, leaf stalks, stems and roots of peanut plants. Thephatogenic test of fungal isolates was conducted to the fifthinstar larvae of Tenebrio molitor and sporulation wasidentified macroscopically and microscopically and alsothe growth rate of the colonies, the conidia population.Based on identification it was found endophiticentomophatogenic from peanut plants. Colonization ofendophitic fungus was highest in leaf stalks and leaves(82.40 and 80.52%).

Biocontrol, colonization, endofitic, entomopatogen

BO-19Odonata diversity in Rawa Bendungan, Cilacap,Central Java

Muhammad Nu'manuddinFaculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.Tel/Fax. +62-274-580839,email: [email protected]

Odonata can be used for bioindicator of aquatic andterrestrial ecosystems. Rawa Bendungan which is a wellknown tourism site in Cilacap, Central Java is also used forirrigation and fishing site. The aim of this study is to learnthe diversity of Odonata in Rawa Bendungan. This studywas conducted at six observation points in January andFebruary 2016. The result showed that 18 species from 3families were found at the observation site. Species ofLibellulidae and Coenagrionidae were the most observed,while only one species of Gomphidae, Ictinogomphusdecoratus, was observed. The species observed weregenerally tolerant of pollution.

Diversity, odonata, Rawa Bendungan

BO-20Some species of the Genera Cobbonchus Andrassy,1958 and Mylonchulus Cobb, 1916 (Nematoda:Mononchida) from South Kalimantan

Abdul GafurFaculty of Mathematics and Natural Sciences, Lambung MangkuratUniversity. Jl. A. Yani Km 36 Banjarbaru 70714, South Kalimantan,Indonesia, email: [email protected]

The present study is a part of a nematode biodiversityinventory project in South Kalimantan. Three knownspecies of mononchids belonging to the generaCobbonchus Andrassy, 1958 and Mylonchulus Cobb, 1916are described from ricefields and peatlands in KabupatenBanjar, South Kalimantan, viz. Cobbonchus indicus Baqri,Baqri & Jairajpuri, 1978; Cobbonchus mauritianus


(Williams, 1958) Clark, 1960; and Mylonchulus brachyuris(Bütschli, 1873) Cobb, 1917. These are first records ofoccurence of the three species in the Province ofKalimantan Selatan and probably in Indonesia.Morphological characters of M. brachyuris generally fit theoriginal description of the species. Meanwhile, thecharacters of specimens of C. indicus and C. mauritianusshow some discrepancies from the corresponding publisheddescriptions, but are here considered intraspecific variations.

Description, identification, Mononchida, predatorynematodes

BO-21Georeferencing orchids specimen history cards inBogor Botanic Gardens to increase their use forconservation efforts

Eka Martha Della Rahayu1,♥, Safran Yusri2

1 Center for Plant Conservation-Bogor Botanic Gardens, IndonesianInstitute of Sciences (LIPI). Jl. Ir. H.Juanda No. 13, P.O. Box 309 Bogor16003, West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥email:[email protected] TERANGI. Jl. Asyibaniah No. 106 Cipayung Depok, WestJava, Indonesia

Orchids are considered valuable plant resource butoverharvesting and habitat conversion have threatened theirpopulation. Bogor Botanic Gardens, West Java (KebunRaya Bogor-KRB) stores millions of plant specimens takenfrom the wild or captivity. Origin of specimens arerecorded in specimen tags and cards, where each of thesecan be converted to species occurrence datum forinvestigations of biodiversity, its relationship with theenvironment, evaluating conservation efforts andanthropogenic disturbances along spatial or temporalscales. However, data from tags and cards available areoften not sufficient because localities have typically beingrecorded as textual descriptions, without geographiccoordinates, thus making analysis using GeographicalInformation System (GIS) tools difficult. In this paper, wereviewed the use of online resources (i.e. GoogleMaps™,ProtectedPlanet.net, OpenStreetMap and OpenSeaMap) forgeoreferencing specimen cards and Quantum GIS as a GIStool to store and display the data. Specimen cards fromchosen genera of orchid in KRB were reviewed. Thegeoreferencing process encountered several obstacles,includes: geographically biased locations, changes inspatial-administrative borders, unregistered location name,unavailability of location name in online resources, andtypographic errors during specimen recording process. Wealso encounters quality difference along georeferencedrecords, some are good quality (i.e. record coordinates ornearest village) and some are poor (only record theprovinces). Georeferencing is an underappreciated task, butonce it is done, it can be used for future expeditionaryresearch, national conservation planning, species statusreview, and other large scale analysis for both spatial andtemporal scales.

Bogor Botanic Garden, georeferencing, orchids, plants,specimen collections

BO-22Rediscovery of Fagraea litoralis (Loganiaceae) inMount Nglanggeran of Baturagung MountainsYogyakarta

Widodo1,♥, Muhammad Ja'far Luthfi2

1 Biology Education Program, Faculty of Science and Technology, StateIslamic University Sunan Kalijaga. Jl. Marsda Adisucipto No. 1 Sleman55281, Yogyakarta, Indonesia. ♥email: [email protected] Department, Faculty of Science and Technology, State IslamicUniversity Sunan Kalijaga. Jl. Marsda Adisucipto No. 1 Sleman 55281,Yogyakarta, Indonesia

Fagraea litoralis population were found in the side ofMount Nglanggeran track in Yogyakarta. Identificationwas based on the literature and herbarium specimens. Thestudy was conducted through continued exploration andexamination using specimen collection. The existence of F.litoralis in Java described in Flora of Java by Backer andBakuizen (1965) were minimum. F. litoralis life in theform of liana creeping large stone. Characteristic foridentification of the initial plant are ovate to ellipsoidleaves, opposite, thick, grayish green color, flower shape ofa bell-trumpet, flowering around March, colors yellow,corolla tube about 5-8 cm. This paper presents importantmorphological characteristic, namely leaves,stems, flowers,and fruits. F. litoralis were found on mount Nglanggeran ofBaturagung mountains Yogyakarta. Recognition andawareness of this plant were needed for its potential,benefit and conservation of this species.

Fagraea litoralis, Loganiaceae, Mount Nglanggeran,Baturagung mountains

BP-01Existence of bats in Mount Walat EducationForest, Sukabumi, West Java

Adheliya Setyorini♥, Sasti Regi BintariDepartment of Biology, Faculty of Mathematics and Natural Sciences,Bogor Agricultural University. Jl. Agatis, Bogor 16680, West Java,Indonesia. Tel./Fax. +62-251-8622833, ♥email:[email protected]

Bats are nocturnal animals that belong to Chiroptera Order.The animal’s role are as fruit’s seeds disperser, plantationpolinator, insect infection controler, and guano fertilizerproducer. The study aimed to observe bats’s existence andidentify bats’s characters in Mount Walat. Bats samplederived from exploration method by using mist net andharp trap. Bats identification carried out based on themethod from Suyanto (2001) and Prasetyo et al. (2011).Morfometric data of trapped bats was measured such ashead and body, weight, forearm, ear, and tail. Temperatureand relative humidity also measured in trap location. Result


showed that there were 5 species from 3 families of batswhich were recorded, namely Pteropodidae (Cynopterusbrachyotis and Rousettus leschenaultii), Rhinolophidae(Rhinolopus pussillus and Rhinolophus affinis), andHipposideridae (Hipposideros larvatus) in Schima wallichiistands. Cynopterus brachyotis was the most recorded batsduring the research.

Bats, Chiroptera, Morfometric, Mount Walat

BP-02Early growth of three Dipterocarpaceace speciesunder rubber plantation in North BengkuluDistrict, Bengkulu Province

Wiryono1,♥, Hery Suhartoho1, Ali Munawar2

1 Department of Forestry, Faculty of Agriculture, University of Bengkulu.Jl. WR Supratman, Bengkulu, Indonesia. ♥email:[email protected] Department of Agroecotechnology, Faculty of Agriculture, University

of Bengkulu. Jl. WR Supratman, Bengkulu, Indonesia

Dipterocarpaceae was the most dominant tree family in theforest of Sumatra and Kalimantan, so the forest was calleddipterocarp forest. However, the massive forestexploitation for several decades have depleted most of thedipterocarpaceae from the forest because the dipterocarptrees have high economic value. Not only dipterocarp trees,but also many other forest tree species have disappearedbecause much of the forest have been converted into singlespecies plantation. The objective of this study was to plantthree dipterocarp spcecies, namely Shorea javanica, Shoreamacrobalanos and Hopea mangarawan, under rubberplantation. The study has been conducted in PondokKelapa Sub-district, North Bengkulu District, BengkuluProvince, Indonesia since February 2015 up to the present.Thirty seedlings for each species were planted regularly,with planting distance of 2 x 2 m2 under Hevea brasiliensisstand. The height and diameter of plants were measuredevery month. The data were analyzed statistically to knowthe difference growth among the three species. Within ayear, Shorea javanica grew 8,6 cm in height (68% of theoriginal height) and 2,2 mm (72%) in diameter, Shoreamacrobalanos 20 cm (89%) and 4,4 mm (217%), andHopea mangarawan 22 cm (62%) and 3,6 mm (87%).There was statistically difference in diameter growthamong species but there was no statistically difference inheight growth among species. During severe drought of2015 some seedlings died.

Dipterocarpaceae, Hopea, rubber plantation, Shorea

BP-03Variability on morphological characters of podassociated with pod shattering resistance insoybean

Ayda Krisnawati♥, M. Muchlish AdieIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Pod shattering is one of the major problems associated withsoybean production during dry season in Indonesia. Theobjectives of the study were to classify the varietaldifference of pod shattering and to identify themorphological pod characters related to pod shattering. Thefield study was carried out in Blitar (East Java, Indonesia)during the dry season 2015. Six morphological traits of podwere studied for their association with pod shatteringresistance in 30 soybean genotypes. The results showedsignificant differences between genotypes for all charactersstudied. The degree of shattering resistance differed amonggenotypes. Shattering percentage ranged from 2.50%(G511H/Argom//Argom-2-1) to 100% (Grobogan) withmean of 30.05%. Based on grouping of screening for podresistance, there was no very resistant variety to podshattering. However, 13 genotypes were found to beresistant, 11 genotypes were moderate, 1 genotype washighly susceptible, and 5 genotypes were very highlysusceptible. The direct effects of the pod wall thickness andpod length on shattering percentage as indicated by pathcoefficient were the highest, while other causal effectswere small. These characters (pod wall thickness and podlength) play role as determinant factors in pod shatteringresistance. Therefore, soybean resistance to pod shatteringcould be enhanced by increasing thickness of the pod wall.

Glycine max; pod characteristics; pod shattering; resistance

BP-04Variability of pod trichome’s density and lengthon several soybean genotypes

M. Muchlish Adie♥, Ayda KrisnawatiIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email: mm_adie@yah

Trichome potentially act as a plant defense against soybeanpod feeders, hence, the identification of trichomevariability on soybean pod become increasingly important.A total of 150 soybean genotypes were identified for itspod trichome length and density. The experiment wasconducted from February to June 2014. All soybeangenotypes were planted in Muneng Research Station,Probolinggo (Indonesia). Identification of trichome lengthand density was performed in Laboratory of Biology,Malang State University, East Java. Soybean pods weretaken from the third nodes at R7 stage. Soybean yield, podtrichome length and density were grouped using clusteranalysis. The days to maturity varied from 72 to 83 days(average 77 days), 100 seed weight ranged from 12.50 to23.50 g (average 16.50 g), seed yield ranged from 1.51 to4.28 t/ha (average 2.90 t/ha), pod trichome length varied


from 56.942 to 176.479 µm (average 112.82 µm), andtrichome density ranged from 12 to 59 per 4 mm2 (average27.64 per 4 mm2). Cluster analysis on 150 genotypes basedon yield, trichome length, and trichome density classifiedsoybean genotypes into five major groups. The first groupconsists of 61 genotypes, and characterized by sparsetrichome. Cluster II consists of genotypes with shorttrichome, whereas cluster III characterized by mediumtrichome length and trichome density. Cluster IV and V,each consists of six genotypes, and characterized bydensely and longer trichomes, respectively. There was nosignificant correlation between trichome density withtrichome length, however, makes it difficult to findgenotype with long and dense trichome. The genotypesfrom cluster IV and V could be used as gene source forfurther improvement of trichome length and density insoybean.

Density, Glycine max, length, trichome

BP-05The diversity and kinship of the swamp buffalo(Buffalo bubalis) Pampangan South Sumatrabased on the characteristics morphologists

Yuanita Windusari♥, Laila Hanum, Rahmat PratamaBiology Department, Faculty of Mathematics and Natural Sciences,Sriwijaya University. Jl. Palembang-Prabumulih Km 32, Indralaya, OganIlir 30662, South Sumatra, Indonesia. ♥email: [email protected]

Swamp buffalo (Bubalus bubalis) Pampangan in SouthSumatra is one of the varieties of native buffalo nativeSumatra. Characteristics of buffalo Pampangan that has abody shape tall and big, black leather, head and ears withlong hair, short horns circular toward the back down, thentowards the circular shape spirals, elbow-shaped body, aslim lead as the type of dairy cows, the udder welldeveloped and symmetrical, and calm temperament. Theresearch method is through direct observation of themorphology of each variant were found to swamp buffaloand then calculate kinship use NTSYS software ver. 2.1.Data quantitative as well as bust, high-shoulder, length ofthe body, long tail, length of the head, width of the headand high hips; while qualitative data are color hair, theshape, and direction of growth of horn is presented intabular form. Observations conducted descriptivemorphological characters that include the charactersstarting at the head, neck, body, tail and legs. Furthermore,the character gained will be given scale comparison of thenumbers 0, 1, 2 and 3 in accordance with the characterpossessed of individual variants swamp. The resultsshowed that there are four variants of swamp buffaloes inthe area Pampangan, South Sumatra. The morphologicalcharacteristics of the fourth variant of Buffalo is body size,hair color and the shape and direction of growth of thehorn. The phylogenetic relationships found in swampbuffalo Lampung and black variants. The correlationcoefficient between 0.57 to 0.8 5 an indication of the

closeness between variants. Similarity values more than 0.5indicate variants derived from a common parent.

Diversity, kindship, NTSYS software, swam buffalo

BP-06Variability of lateral shoot and root formation ofin vitro culture of sengon (Paraserianthesfalcataria)

N. Sri Hartati, Dody PriadiResearch Centre for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, Jawa Barat, Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email:[email protected]

Sengon (Paraserianthes falcataria L. Nielsen) is one of thefast growing tree species, which is economically importantas multi purpose source of various wood-based product. Inorder to obtain of high quality seeds that powerfullysupports the productivity of the forestry sector, in vitropropagation of sengon of commercially and superior seedssources was developed using stem segment as en explant.The aim of this study was to determine the growth responseparticularly lateral shoot and root formation of regeneratedstem segment explant of different types of seeds source onMS hormone free medium. The growth response of threetypes of 5 months old plantlets was identified in SI(commercial seed), SII (collected from superior tree), SIII(collected from superior tree). In general, comparablegrowth response in term of plant height and vigor wasobserved in three types of plantlets. The plantlets ofsuperior tree as seeds source (SII and SIII) indicated lowervalues (16.9% and 66.67%) in lateral shoots formationcompared to SI (69.23%). Sengon plantlets of SIII shownthe highest rate formation (85.71%). Plant formed lateralshoots (branches) that initiated in the axil of the leaves orstem nodes. The growth of axillary meristem in most plantsis initially controlled by the shoot apical activity.Decreased branching has been one of the major traits to beselected of tree species cultivation to support high qualitywood production. It needs further field evaluation of thelower shoot lateral production of SII and SIII in associationwith high quality seedlings selection.

In vitro, lateral shoot, Paraserianthes falcataria, sengon

BP-07Development potential of Citrus cv. NimasAgrihorti as biopharmacy citrus

Emi Budiyati♥, Joko Susilo Utomo, Anis AndriniIndonesian Citrus and Subtropical Research Institute. Jl. Raya TlekungNo.1, Tlekung, Junrejo, Batu, East Java, Indonesia, ♥email:[email protected]


Nimas Agrihorti is a citrus crop suitable to develop inIndonesia in order to fulfil the needs of bio pharmacy citrusin domestic markets. This is based on a high quality ofNimas Agrihorti that is considered able to play as the rawmaterial for bio pharmacy industry as more people concernof their health necessity. The objective of this study was toacquire some information of the superiority of NimasAgrihorti’s characteristics and potentials as bio pharmacycitrus. The methodology used was by examination andobservation conducted on June 2013-October 2014 atexperimental field of Banjarsari, Bayeman village,Probolinggo in East Java. Morphological observation of thecrop’s performance was done based on Descriptor List forCitrus (IPGRI), whereas characterisation of fruit’schemical nature was done at the Post Harvest Laboratory ofBrawijaya University, Malang, East Java. The resultsshowed that according to the physical quality (size, colourand taste), Nimas Agrihorti has potential competitiveadvantages for development. It has big-sized fruit of 72-82g in weight, yellow skin colour, sweet taste, 34.8 mg/100 gof vitamin C content and low acid content of 0.45%.

bio pharmacy, citrus, development, variety, Nimas Agrihorti

BP-08Typical of mangrove forests in lagoon areas ofsouthern coast of Java, Indonesia

Ahmad Dwi Setyawan1,♥, Ari Susilowati1, Ari Pitoyo1,Jean W.H. Yong2,♥♥

1Department of Biology, Faculty of Mathematics and Natural Sciences,Sebelas Maret University. Jalan Ir. Sutami 36A Surakarta 57126, CentralJava, Indonesia. Tel./fax. +62-271-663375, email: [email protected] Singapore University of Technology and Design, 20 Dover Road,Singapore 279623, Singapore. email: [email protected]

The rivers on the south coast of Java is forming lagoons atthe estuary. The size of lagoon is generally not extensiveand tend to dry up in the dry season. The mangrove forestis still found in the lagoons, as either a relic habitat or newreplanting. This study aimed to determine the forestsprofile of mangrove in lagoons of the south coast of Java.The study was conducted in the estuary of Bogowonto andOpak rivers, Yogyakarta, Indonesia on November 2015.The results showed that Avicennia and Rhizophora is thedominant species in both places. Forest profiles diagramshowed that Avicennia grows better than Rhizophora withhigher size. This contrasts with the growth of mangroveforests in estuaries of large rivers in Sumatra, Kalimantanand Papua where Rhizopora grows higher than Avicennia.This is a form of local adaptation where Avicennia growsbetter than Rhizophora on the environmental conditions ofwater shortages.

Drought, forest profile, lagoon, mangrove, Southern Java

Diversity of ecosystem

CO-01The roles of community fruit garden (tembawang)on maintaining vegetation structure, diversity andstanding biomass allocation: an effort on reducingcarbon emission

Dwi Astiani♥, RipinFaculty of Forestry, Tanjungpura University. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, email: [email protected]

Fruit garden (tembawang) Desa Cempedak in Sanggau,West Kalimantan has been established by local communityfor their family mixed garden ~ >100 years. The families of3rd generation were utilizing the tembawang for their needsof building materials, fire woods, rattan, vegetables, fruits,and traditionals medicine. It is important to study how thistembawang play their role in maintaing forest structure,species diversity and stocking biomass. In 2014, we studiedthis area for exploring the vegetation composition and theirability to stock biomass of the tembawang. StratifiedRandom Sampling was applied to the 6.69 ha tembawangarea, which devided into three major land cover (mixedfruit garden, mixed rubber plants, and mixed with apikpalm). We surveyed and sampled vegetation using transectmethods purposively on each landcover, with samplingarea consecutively for mixed fruit garden, mixed rubberplants, and mixed of fruit garden and apik were 1.52, 0.6,and 0.72 hectars. Results demonstrated that mixed fruitgarden carry out higher tree composition, density, basalarea, and maintain the largest above ground biomass perhectar compared to two other land covers. It maintain 49tree species on diameter >20 cm and 51 species in thelowest strature in the forest structure. Interestingly, Duriozibethinus Murr. was a dominant species on all tree majorland covers and hold the largest above ground biomass.Thechoice of fruit species on tembawang determine thecapability of the land to sequester and stock carbon withintrees, because the trees were standing in tembawang forlonger time compared to production forest, tembawangprovide other benefit to the nature

Above ground biomass, carbon stocks,.lowland tropicalforest, mixed fruit garden.

CO-02Choosing native tree species for establishing man-made forest: A new perspective for sustainableforest management in changing world

Atok Subiakto1,♥, Henti Hendalastuti Rachmat2,♥♥

1 Forest Research and Development Center, Ministry of Environmentaland Forestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, JawaBarat. Tel. +62-251-8633234; 7520067. Fax. +62-251 8638111. ♥email:[email protected].


2 Forest Fiber Technology for Research Plantation. Jl. Raya Bangkinang-Kuok Km 9, Kotak Pos 4/BKN Bangkinang 28401, Riau, Indonesia. Tel.:+62-762-7000121, ♥♥email: [email protected]

Establishment of tree plantation on degraded lands andforest clearly favoured some exotic species such asGmelina arborea, Acacia, and Eucalyptus. Highproductivity, less harvesting time, and deeper silviculturalknowledge are the beneficial factor for choosing thoseexotics species. However, the use of a wide variety ofnative tree species becomes more significantly important inreforestation projects due to the greater biodiversitybenefits and wider environmental services. This researchwas carried out as a multiyear observation and continuousexperiment to value how native tree resources can be aprospective alternative in providing and supporting humangrowth and needs. The performances of two nativeIndonesian Shorea species, Shorea leprosula and Shoreaselanica, were evaluated at a dipterocarp planting trial intwo different sites in Indonesia. Growth data was obtainedfrom 16 years old plots, twelve 100 m X 100 m squareplots on mineral soils (Gunung Dahu ExperimentalForest/GDEF, Bogor) and eight resembled plots onfrequently flooded peat land (PT. Arara Abadi/PT. AA,Riau). Survival rates were varied, ranged from 36 to 77%,diameter at breast height from 13.7 to 24.9 cm, tree heightfrom 10.8 to 16.9 m, mean volume from 0.119 to 0.567m3/tree, and total volume from 79.420 to 215.412 m3/ha.Growth rates of planted saplings were affected by species,site and spacing distance. The market value of dipterocarpswood is about twice the value of exotic fast growing tree.Therefore, development of man-made native dipterocarpsforest in the tropic, especially in South East Asia can be asprospective as developing an exotic fast growingplantation. Hence, establishing man-made dipterocarpforest can maintain and support the genetic conservation ofthe native species with lower to no risk of species invasioncompare to those of developing exotic trees plantation.

Exotic species, native tree, plantation, Shorea leprosula,Shorea selanica

CO-03The diversity of plant species, the type of plantuses and the estimate of carbon stock inagroforestry system in Harapan Makmur Village,Pondok Kubang Sub-district, Central BengkuluDistrict

Wiryono1, Venny Utami Puteri2, Gunggung Senoaji1

1 Department of Forestry, Faculty of Agriculture, University of Bengkulu.Jl. WR Supratman, Bengkulu, Indonesia. ♥email:[email protected] The Office of Forestry, Plantation, Mining and Energy, Empat Lawang

District, South Sumatra Province, Indonesia

Agroforestry system is usually found in traditional homegardens in rural areas in Indonesia, where a variety ofagriculture and forestry plant species are grown in a mixedplantation. To some extent, the traditional home gardens

resemble natural forest in vegetation structure andcomposition. The objective of this study was to know thediversity of plant species, the types of plant uses and theestimate of carbon stock in home gardens in HarapanMakmur Village, in Pondok Kubang Sub-District, CentralBengkulu District, Bengkulu Province, Indonesia. The fieldwork was conducted from April to May 2013. Data of plantdiversity were collected from sampling in quadrats, eachmeasuring 10 x 10 m2 for trees, 4 x 4 m2 for shrubs andsaplings, and 1 x 1 m2 for herbs, with sampling intensity of25%. A total of 23 home gardes were selected. The datarecorded were plant species, plant diameter at breast heightand types of plant uses. Data of plant uses were collectedthrough interview. The data were analyzed to determine thediversity index, importance value index, estimate of carbonstocks, and types of uses. The results showed that 69species of plants were found in the home gardens, with aShannon-Wiener diversity index of 0.99 for trees. The mostdominant species of trees was Hevea brasiliensis Willd(rubber tree) with an importance value index of 127%. Forsaplings (small trees) and shrubs, Hevea brasiliensis Willdwas also the most dominant with 169 individuals, while forherbs, Agrostis sp was the most dominant species with anaverage coverage of 25.85%. The community used manyplants species for several purposes: 41 for food, 11 formedicine, 21 for fire wood, 2 for handy craft, 4 for fences,3 for forage, 13 for ornamental plants, 6 for shade trees,and 2 for coloring. Twenty three species were not used.The estimate of carbon stock in the trees was 81.32 ton/ha.

Agroforestry, home gardens, plant diversity, carbon stock

CO-04Diversity of faunal communities in the BiodiversityPark of Aqua Danone Ciherang, Bogor, West Java

Hendra Gunawan1,♥, Sugiarti2,♥♥, Anita Rianti1, VivinSilvaliandra Sihombing1

1 Forest Research and Development Center, FORDA-Ministry ofEnvironment and Forestry, Jl. Gunung Batu No. 5 Bogor. Kotak Pos 165Bogor 16610, West Java, Indonesia. Tel.: +62-251-8633234, 7520067,Fax. +62-251-8638111, ♥email: [email protected] Center for Plant Conservation-Bogor Botanic Gardens, IndonesianInstitute of Sciences (LIPI). Jl. Ir. H.Juanda No. 13, P.O. Box 309 Bogor16003, West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥♥email:[email protected]

A Biodiversity Park is a new method for ex situconservation in Indonesia. The concept was first launchedin 2012. The purposes of a Biodiversity Park are toconserve indigenous and threatened species of flora,provide habitat for a diversity of animal life, and to providelocal residents with opportunities for economic benefit,recreation, education and research. One of the goals ofBiodiversity Parks is to improve the diversity of flora andfauna in the midst of human settlement and industrialprecincts. This research was directed at studying thediversity of faunal communities in the Biodiversity Park ofAqua Danone Ciherang, Bogor. Line transects, walktransects, terrestrial transects and point count methods were


combined to census the mammals, reptiles, amphibians andbirds in the Biodiversity Park. Twenty five families offauna were identified, consisting of 28 genera and 32species. The Shannon diversity index for the total faunalcommunity was 2.82. Composition of the faunalcommunity consisted of birds (66%), reptiles (16%),mammals (12%) and amphibians (6%). This findingsupports the concept that Biodiversity Parks cansuccessfully provide habitat for diverse fauna. Thediversity index of 2.37 for the bird community indicates abeneficial contribution to habitat quality within an urbanenvironment.

Biodiversity Park, fauna, habitat, green space

CO-05Ex situ conservation of North Sumatran MountainFlora at Samosir Botanic Garden

SugiartiCenter for Plant Conservation-Bogor Botanic Gardens, IndonesianInstitute of Sciences (LIPI). Jl. Ir. H.Juanda No. 13, P.O. Box 309 Bogor16003, West Java, Indonesia. Tel./fax.: +62-251-8322187. email:[email protected]

The tropical rain forest of Sumatra with its richness ofbiodiversity was designated as a world heritage location byUNESCO in 1992. However, degradation and deforestationhas continued, causing significant biodiversity loss.Therefore, in situ conservation through the establishment ofprotected areas is not enough. Another strategy ofconservation action, in parallel and synergy, is needed toprotect biodiversity from extinction. Ex situ conservationthrough development of local botanic gardens is anothersolution to protect native flora, particularly, endemicspecies from extinction. Samosir Botanic Garden wasestablished in 2010 by the decree of Samosir Regent no.19, to cover an area of 100 ha. It has aims to conserve themountain flora of North Sumatra. The method fordevelopment of Samosir Botanic Garden includes planning,implementation, monitoring and evaluation. Exploration ofthe mountain forest of North Sumatra is being carried outto collect local, rare and endemic flora. The collections arebeing planted at the Garden. By the end of 2015, SamosirBotanical Garden already had a collection of 99 species offlora planted out in the Garden. Another 155 species arestill being prepared in the nursery. The planting concept isdesigned according to thematic gardens, such as a BatakEthnobotany Garden, a Mandailing Natal Garden and anOrchid Garden. Besides being a conservation area, SamosirBotanic Garden also functions for research, education andecotourism. This is in line with the national program thathas declared Lake Toba to be a National Strategic Area(Kawasan Strategis Nasional) as a tourist destination and asa proposed Geopark.

Conservation, flora, ex situ, Samosir, Botanic Garden

CO-06Climate-induced hydrological changes and theecology of freshwater Biota: A review

Sunardi1,2,♥, Gerhard Wiegleb3

1 Department of Biology, Faculty of Mathematics and Natural Sciences,Padjadjaran University. Jl. Raya Bandung-Sumedang Km 21, Jatinangor,Sumedang 45363, West Java, Indonesia. Tel.: +62-22-7797712 line. 104,Fax.: +62-22-7794545, ♥email: [email protected] Institute of Ecology, Padjadjaran University. Jl. Sekeloa Selatan No. 1Bandung, West Java, Indonesia3 Department of General Ecology, Faculty of Environmental Science andProcess Engineering, Brandenburgische Technishe Universitat (BTU)-Cottbus-Germany

Climate change is believed to pose adverse effects tobiodiversity of aquatic systems, both in boreal and tropicalareas. The tropical freshwater systems are expected tosuffer more severe impacts of climate change, from heavyfloods or extended drought than do the boreal areas.Unfortunately, next few decades species extinction issuggested as dark future as we lack researches uncoveringhow climate change threats the aquatic biota. Therefore, acomprehensive understanding of biota’ performance in faceof climatic pressures, will guide the further necessaryresearches. This paper presents a review on the availableresearches addressing ecological effects of the mostinfluential climatic parameters, flood and drought, onfreshwater biota.

Biota performance, climate change, drought, flood,freshwater ecosystem

CO-07Status of population, occupation and seasonalhabitat displacement of alien bird species in WestJava tropical forest

Ruhyat PartasasmitaDepartment of Biology, Faculty of Mathematics and Natural Sciences,Padjadjaran University. Jl. Raya Bandung-Sumedang Km 21, Jatinangor,Sumedang 45363, West Java. Phone. +62-22-7797712 line. 104, Fax. +62-22-7794545, ♥email: [email protected]

“Invasive species”, an alien species is defined as one “thatis not native” to a particular ecosystem. One cause of aspecies alien species is introduced intentionally orunintentionally. Trade of animals including birds,indicating the cause of alien species in several regions inIndonesia. it is suspected as the discovery of a populationof Finch-billed Myna”Scissirostrum dubium Latham 1802”in the forests of Western Java as a result of the indirectinfluence of the bird trade. Finch-billed Myna is one ofmany birds endemic in Indonesia. In 2006, this birdcategored as threatened species by IUCN. As far as weknow, finch-billed Myna is resident bird in Sulawesi islandand sorrounding. CITES entered it as least concent speciesbecause few information about population anddistribustion. The other hand, We found them inTangkuban Parahu Mount area. The population of Finch-


billed Myna In Tangkuban Parahu Mount is 17-24 individuof three group colonies. The occupation areas are Abria,Awi leuga and Manggu. The vegetation density higher atMyna’s former habitat (0.055 ind./m2) and Myna’s recenthabitat (0.028 ind./m2). Vegetation of Myna’s recenthabitat have good resources as resting site, feeding site, andperching site of dead trees that Myna’s former habitat.

Finch-billed Myna, habitat displacement, occupation,population

CO-08Biological treatment with consortia of Mn2+ andFe2+ oxidizing bacteria from alfisol Jumantono forgroundwater purification

Vita Ratri Cahyani♥, Sumani, Dwi Priyo Ariyanto, AyuIndah SaputriFaculty of Agriculture, Sebelas Maret University. Jalan Ir. Sutami 36ASurakarta 57126, Central Java, Indonesia. Tel./Fax. +62-271-637457♥email: [email protected]

Biological oxidation process was proposed as an alternativemethod for water purification. The study aimed atelucidating bacterial consortia that were potential to beapplied as effective biological oxidants on the separation ofMn2+ and Fe2+ in groundwater purification. Three steps ofmethod were conducted. The first was isolation of Mn2+

and Fe2+ oxidizing bacteria from the precipitation layer ofMn and Fe and from groundwater of Alfisol Jumantono.The second was examination of the oxidation type of theisolates of Mn2+ oxidizing bacteria, and the third wasinvestigation of the oxidation capability of the consortia ofMn2+ and Fe2+ oxidizing bacteria in the simple design ofgroundwater purification process. The results showed thatthe consortium of Mn2+-oxidizing bacteria with oxidationtype III indicated the highest capability in Mn oxidationcompared with the types I and II, whereas the consortiumof Fe-oxidizing bacteria of Leptothrix strains indicatedhigher capability in Fe oxidation than Sphaerotilus strains.The decrease of soluble Mn in all treatments did not reachto the allowed level for drinking water. The decrease ofsoluble Fe in all treatments with application of Leptothrixstrains reached to the allowed level of 0.25-0.28 mg/L.Combination of consortia of Mn2+ and Fe2+-oxidizingbacteria indicated decreasing soluble Mn and soluble Fe atthe same level with the results from consortia of Fe2+-oxidizing bacteria only. It could be concluded that theeffect of Mn2+-oxidizing bacteria was minor compared tothe effect of Fe2+-oxidizing bacteria.

Biological treatment, consortia, Fe2+oxidizing bacteria,groundwater purification, Mn2+ oxidizing bacteria

CO-09Effect of arbuscular mycorrhizal inoculumoriginated from Surakarta Area and rockphosphate toward P uptake of corn on alfisolJumantono

Vita Ratri Cahyani♥, Sudadi, Diyah Ratri PalupiFaculty of Agriculture, Sebelas Maret University. Jalan Ir. Sutami 36ASurakarta 57126, Central Java, Indonesia. Tel./Fax. +62-271-637457♥email: [email protected]

The effectivity of arbuscular mycorrhiza (AM) asbiofertizer are determined by internal characters of AM andvarious environmental factors. The effectivity of AM is notalways parallel with their infectivity in plant root. Thepresent research aimed at elucidating the infectivity andeffectivity of arbuscular mycorrhizal (AM) inoculumoriginated from a variety of soil types in Surakarta and theeffect of rock phosphate with two dosage levels toward Puptake of corn on Alfisol Jumantono. The research wasconducted in green house by applying AM inoculum from:Andisol Tengaran, Alfisol Jumantono, Entisol Mojosongo,Inceptisol Bengawan Solo, Vertisol Jatikuwung, and rockphosphate with levels of dosage consisted of: 0 kg ha-1;682 kg ha-1; 1364 kg ha-1. The results indicated that AMinoculum from Andisol Tengaran showed the highestinfectivity and effectivity comparing with inoculum fromother soil sources. The treatment of rock phosphate withdosage of 682 kg ha-1 resulted in the highest infection ofAM and the number of mycorrhizal spores comparing withdosages of 0 kg ha-1 and 1364 kg ha-1. The interaction ofAM inoculum from Andisol Tengaran and rock phosphatewith dosage of 682 kgha-1 showed the highest infection ofAM of 86.67% (38.3% higher than control), the interactionof AM inoculum from Entisol Mojosongo, and rockphosphate with dosage of 682 kg ha-1 showed the highestavailable P2O5 30.17 ppm (133.89% higher than control),and interaction of AM inoculum from Andisol Tengaranand rock phosphate with dosage of 1364 kg ha-1 showedthe highest P uptake of corn 1.13 g plant-1 (151.11%higher than control). The correlation analysis test indicatedthat P uptake of corn was significantly correlated with theinfection of AM (P-value = 0,000).

Alfisol Jumantono, arbuscular mycorrhiza, corn, P uptake,rock phosphate, Surakarta

CO-10Mangrove health index as part of sustainablemanagement in mangrove ecosystem atKarimunjawa National Marine Park, Central Java

Johan Danu Prasetya1,♥, Ambariyanto2, SupriHaryono2, Frida Purwanti2

1 Environmental Engineering Study Program, Faculty of MineralTechnology, Universitas Pembangunan Nasional “Veteran” Yogyakarta.Jl. SWK 104, Condongcatur, Sleman 55283, Yogyakarta, Indonesia.Tel./fax. +62-274-485705. ♥email: [email protected] Faculty of Fisheries and Marine Sciences, University of Diponegoro, Jl.


Prof Soedarto SH, Tembalang Campus, Semarang 50275, Central Java,Indonesia

Monitoring the health of marine ecosystems is an integralpart of sustainable management of the ecosystemsincluding mangrove. Distribution and diversity ofmangrove ecosystems are two important parameters inMangrove Health Index (MHI). The index analyses thevalue of mangrove density, diversity index, index ofuniformity and the number of mangrove species. This studyinvestigates mangrove distribution and diversity to supportssustainable management at Karimunjawa National MarinePark. Mangrove distribution maps were prepared usingSatellite imagery GEO-EYE. Analysis of satellite imagerywas carried out at several islands i.e. Cemara Kecil,Cemara Besar, Krakal Besar, Krakal Kecil, Cilik, Sintok,Menjangan Besar, Menjangan Kecil, Tengah andKarimunjawa with a total area of 3752 ha. These werefollowed by ecological surveys to analyse the diversity ofmangrove. at five locations, namely Legon Cilik, LegonGede, Kemujan, Karimunjawa and Menjangan Besar.Satellite image interpretation results showed that mangrovewere found at island of Karimunjawa and MenjanganBesar. In total, 22 mangrove species identified, 12 specieswere true mangroves and the rests were mangroveassociates. Rhizophora apiculata dominates three of ninesurvey locations, while Rhizophora stylosa was thedominant species in two of nine locations and the rest aredominated by Ceriops tagal, Lumnitzera racemosa,Rhizophora mucronata and Sonneratia alba. In the categoryof tree (diameter > 4cm), mangrove cover ranging frommedium to high, from 1400 to 2700 ind/ha. The highest andthe lowest mangrove cover were found at Kemujan 2 (2700ind/ha) and Legon Cilik 1 also Karimunjawa 2 (1400ind/ha). In general, the Shannon diversity index was low.Mangrove in Legon Cilik 1 had the highest Shannondiversity index (1,09) while Menjangan Besar were thelowest (0,19). In the category of sapling (height>100 cmand diameter <4 cm), dominant mangrove species wasrelatively diverse. Mangrove cover ranging from low tohigh category (800-7200 ind/ha). The result also shows thatthe value of MHI at Karimunjawa was 200, which is in themedium category. The results suggest that there is a need toimprove MHI as part of sustainable management based ondistribution and diversity of mangrove.

Distribution, diversity, Mangrove Health Index, sustainablemanagement, Karimunjawa Islands

CO-11Indigenous Kaliwu agro-ecosystem, biodiversityconservation and environmental service on SumbaIsland, East Nusa Tenggara

Gerson N. NjurumanaForestry Research Institute of Kupang. Jl. Alfons Nisnoni (UntungSurapati) No. 7 Airnona 85115 Kupang 85115, Nusa Tenggara Timur.Tel. +62-380-823357, Fax. +62-380-831068, email:[email protected]

Indonesia is a mega-biodiversity country in biologicalresoucers and cultures. Biodiversity and culturesassimilation construct a varies specific models on naturalresource management, one of which is indigenous agro-ecosystem. Indigenous agro-ecosystem has a strategicvalue in support the national development, so it needs to bedeveloped. The study aims to investigate the characteristicsof Indigenous Kaliwu Agro-Ecosystem (IKAS) on Sumbaisland, and its role in biodiversity conservation andenvironmental services. Ethnoecology approach, interviewsand field observations is used, data analysis withdescriptive and qualitative. The results of reserach showed:(i) IKAS has specific of biophysical characteristics thatsupport the hills conservation, (ii) IKAS has a importantrole in biodiversity conservation, mainly as a habitat of 145species of plants, and (iii) IKAS have environmentalservice benefits as a source of food, forage, timber and fuelwood. It was concluded that IKAS has biophysicalcharacteristics with positive implications for thebiodiversity of plants, and a varies of environmentalservice benefits for humans and nature conservation.

Agro-ecosystem, biodiversity, environmental service

CP-01Isolation of Actinomycetes from mangroveecosystem in Torosiaje, Gorontalo

Abubakar Sidik Katili♥, Yuliana RetnowatiCoastal Ecology Based Local Wisdom Research Center, DepartmentBiology, Faculty of Mathematics and Natural Sciences, State Universityof Gorontalo,Jl. Jendral Sudirman No. 6 Kota Gorontalo, Gorontalo Province,Indonesia, Tel. +62- 435-821125, Fax. +62-435-821752. ♥email:[email protected]

Actinomycetes are a group of gram-positive bacteria,known as a producer of secondary metabolites thatimportant in the industrial world. The secondarymetabolites have activities as antibacteria, antifungal,antiviral, and anticancer. Actinomycetes are widespread inthe environment of terrestrial, freshwater and marineenvironments. Actinomycetes in marine environmentalstudies, especially mangrove swamps still very littlecompared to the terrestrial environment. The research aimsto obtain isolates of actinomycetes from mangrove plantrhizosphere sediment in mangroves Torosiaje which will bean opportunity to explore secondary metabolites/bioactivecompounds that support the industry. Research succeed toget seven isolates actinomycetes from five kind ofrhizosphere mangrove sediment, ie Ceriops tagal,Bruguera gymnorizha, Xylocarpus sp., Avicennia marinaand Rhizophora apiculata.

Actinomycetes, mangrove, marine environments


CP-02Farmer adaptation strategy in paddy field affectedby climate variability in monsoon regions

Yayan Apriyana♥, Yeli Sarvina, Elsa Rakhmi Dewi, andAris PramudiaIndonesian Agroclimate and Hydrology Research Institute (IAHRI). Jl.Tentara Pelajar No.1A, PO. BOX. 830 Kampus Penelitian PertanianCimanggu Bogor 16111, West Java, Indonesia. Tel. +62-251312760,♥email: [email protected]

Adaptation of agriculture cultivation to climate variabilityand climate anomalies both in paddy field especially inmonsoon regions is a one of strategy to minimize impact ofthese two phenomena in order to reduce the economic lost,particularly for food security in Indonesia. The objectivesof this study are: (i) to identify onset and cropping patternin irrigated land, rainfed and dry land in affected area dueto climate anomalies. (ii) to collate information of carryingcapacity water resources and adaptation practices appliedby farmers (iii) to identify strategies for farmers onirrigated land, rainfed and dry land in the region affectedby climate variability. The desk work analysis and fieldsurvey were conducted in Serang District, Banten Province,Subang District, West Java Province and Pati District,Central Jawa. This study includes: (i) The correlationanalysis climate anomaly and rainfall, (ii) field survey, (iii)analysis onset growing season, cropping pattern, wateravailability, the best planting time and irrigation schedule.The results showed that the farmers in affected area due toclimate variability could adapt by shift the onset ofgrowing season. Farmer in irrigated land shift their onsetaround 2-4 tendays period becomes October II-DecemberII, in rainfed area around 4-6 tendays period becomesNovember I-January III and in dry land around 6-8 tendaysperoid from November II-Februari I. The cropping patternrice-rice-palawija/fallow was applied in irrigated land, rice-rice/palawija/fallow-fallow in rainfed and palawija-palawija/fallow-fallow in dry land. Adaptation programs todeal with climate variability in Serang and Pati varies morethan in Subang. In Serang and Pati, during first growingseason farmers applied irrigation roughly 20%-30% pumpwater from river and during second growing season, in Patifarmers use water from well pump and even in Serangreach 100%.

Adaptation strategy, climate variability, farmer, monsoonregions, paddy field

CP-03Management leadership in the promotion ofvillage farmer softskill information technology inthe Province of Gorontalo

Novianty DjafriManagement Education Department, Faculty of Education, StateUniversity of Gorontalo. Jl. Jendral Sudirman No. 6, Kota Gorontalo,Gorontalo, Indonesia. Tel. +62- 435-821125, Fax. +62-435-821752,♥email: [email protected]

Government as a leader in protecting all people indeveloping economies in the region. Government withrelated parties should be able to prosperity and welfare ofthe community, in addition to master the administrativearrangements for the control of managerial competence,should be able to understand the functions of management,and also in the field of entrepreneurship. Leader in amanagement capacity must be at prop with socialcompetence to be able to protect its people, especially thefarmers. As a leader must master the science ofmanagement in the system of administration and control forsetting the Class Action for farmers. Leader in advancingmanagement function can run the policies and rules thathave been agreed in the region. Management Leadership insoft skills coaching can be an underlying implementation ofmanagement functions, the concept of nurturing thoughtleadership and supported by information systems that canbe directly received by farmers. For the province ofGorontalo, coaching by the Regional Head (Governor) tofarmers in the implementation of the managementfunctions of an underlying concept of leadership andleadership areas that: (i) Planning of the government in theform of socialization (ii) Organizing farmers through ofscientific Forum. (iii) Mobilizing Farmers marketcompetitiveness through (iv) Supervise the farmers withindependence through increased resale value through theutilization of information technology. Commitment andconsistent enforcement continues to be undertaken by theGovernment of Gorontalo province through the efforts andstrategies to increase potential, productivity and innovationof the agricultural community, given the farmingcommunity has an important role Gorontalo be driving theregional economy and become identity regional pride. Thedata used is the survey data, the secondary and primarydata were obtained in the field and study the results ofprevious research literature. It is built is through qualitativeand quantitative data that are presented descriptively. Theresult of this research is the leader in the GuidanceManagement Forms Softskill Farmers through Utilizationof Information Technology.

Information Technology, management leadership, soft skilldevelopment

Ethnobiology

DO-01The local knowledge of the rural people on species,role and hunting of birds: Case study inKarangwangi Village, Cidaun Sub-district, WestJava

Johan Iskandar1,2,♥, Budiawati Supangkat Iskandar3,♥♥,Ruhyat Partasasmita1,♥♥♥


1 Biology Department, Faculty of Mathematics and Natural Sciences,University of Padjadjaran. Jl. Raya Bandung-Sumedang Km 21,Jatinangor, Sumedang 45363, West Java, Indonesia. Tel.: +62-22-7796412. ♥email: [email protected]♥♥♥ email: [email protected] Postgraduate of Environmental Study (PSMIL and DIL) and PPSDAL,Padjadjaran University. Jl. Raya Bandung-Sumedang Km 21, Jatinangor,Sumedang 45363, West Java, Indonesia3 Lecturer staff at the Anthropology Department, Faculty of Social andPolitical Science, Padjadjaran University. Jl. Raya Bandung-SumedangKm 21, Sumedang 45363, West Java, Indonesia. ♥♥ email:[email protected]

Based on the ecological history, in the past many villagesof West Java had a high diversity of birds. Nowadays,however, the diversity of birds in some villages of WestJava has tended to decrease due to many factors, includingintensive illegal bird hunting which has been undertaken bysome bird hunters. The objective of this paper is toelucidate the local knowledge of Karangwangi village,West Java on species, role, and hunting of birds. Methodwas used in this study is the qualitative with descriptiveanalysis which the ethnoornithological approach is applied.Results of study show that the Karangwangi people have avery good knowledge on bird species, particularly on levelspecies/specific. Various bird species are traditionallyclassified into seven categories (folk classification), namelybased on distinctive voice, characteristic morphology,special colour, momentary activity, special commonhabitat, nest characteristics, and role in the ecosystem.Based on the rural people perception, the role of birds canbe divided into two categories, namely notorious andbeneficial birds. The diversity of rural birds has tendeddecrease over time due to various factors, including illegalbird hunting for various purposes of the village people,such as keep a bird in cages and bird trading. The studysuggests the perception of rural people on birds havechanged caused of socio-economic and cultural changes.Nowadays the bird hunting in the rural area has tended toshift from a purely subsistence form towards a morecommercial form and, thus, to conserve bird species thesocio-economic and cultural rural people aspects might beintegrated to national as well as international birdconservation programmes.

Bird classification, bird hunting, ethnoornithology,Karangwangi village, local knowledge

DO-02Ethnobotany of traditional healer (dukun) inGiam Siak Kecil Bukit Batu Biosphere ReserveRiau Province

Prima Wahyu Titisari1,♥, Tati Suryati Syamsudin2,♥♥,Achmad Sjarmidi2,♥♥♥

1Departement of Biology, Faculty of Teacher Training and Education,Riau Islamic University. Jl. Kaharuddin Nasution No. 113 MarpoyanPekanbaru. Tel.: +92-761-674674. ♥ email: [email protected] of Biology, School of Life Sciences and TechnologyBandung Institute of Technology, Jl. Ganesha Bandung, West Java,Indonesia. ♥♥ email: [email protected], ♥♥♥ email:[email protected]

Most of the people in Giam Siak Kecil, Bukit BatuBiosphere Reserves still believe in traditional healers(dukun) to treat their disease. This research aims to: (i)describe plant species dukun practice, (ii) understandingthe strategy dukun culture in maintaining its existence. Thisresearch located in Giam Siak Kecil Bukit Batu BiosphereReserves and take the informants purposively, whichconsists of traditional healers and the people who went tothe patient. Technique of collecting data doing throughparticipate observation and in-depth interviews, while thedata analyses by ethnographic and ethnobotany descriptive.The results showed, dukun practice exists because peoplestill believe in the traditional medical. Dukun can treatmedical and non-medical desease (such as form of jininterference and demons) with traditional ways such asprayer, water-filled prayers, potions from herbs, or pressingthe nerve points on the body, and supernatural powers.Recorded 110 species of plants and more than 57 familiesare utilized for traditional medicine. Dukun alsoimplemented several strategies to maintain the culture ofthe patient. Traditional medicine needs to be preservedbecause it is one of the local wisdom.

Dukun, traditional healers, traditional medicine

DO-03Nature conservation through local wisdom: therole of sacred places in western slope of MountLawu in preserving rare trees

Nor Liza♥, Muhammad Arif Romadlon, MuhammadRidwan, Anisa Septiasari, Irmayanti Sari Dewi, ZenitaMila Lutfia, Krisanty Kharismamurti, Dafi Al. Anshori,Rizma Dera Anggraini, Rekyan Galuh Witantri, DwiSetyo Saputro, Yudha NovianaDepartment of Biology, Faculty of Mathematics and Natural Sciences,Sebelas Maret University. Jalan Ir. Sutami 36A Surakarta 57126, CentralJava, Indonesia. Tel./Fax. +62-271-663375, ♥email:[email protected]

Traditional knowledge and local wisdom of Javanesepeople offer alternative perception on ecologicalunderstanding, include the existence of sacred places in thepreservation of rare plants. Mount Lawu is one of the mostsacred mountain with a lot of historical sites. The aim ofthis study was to inventory the diversity of conservedplants in hieratic sites in western hillside of Mount Lawu.Interview key informants and households, and fieldobservation were conducted to obtain data. In total wenoted 16 hieratic sites scattered in western hillside ofMount Lawu that classified into four categories, i.e.shrines, springs, graves, and sacred trees. About thirty nineplants species were recorded around the hieratic places andamong them there are 10 rare trees species. From this studywe can conclude that sacred values could support theconservation effort because it prevents people to harm theenvironment.

Local wisdom, nature conservation, sacred places


Bioscience

EO-01Effects of paclobutrazol on growth and yield ofseveral potato varieties grown at mid elevationarea

Kumala Dewi1,♥, Fransiscus Xaverius Wagiman2, DidikIndradewa2, Erlin Kurniati3

1 Faculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.Tel/Fax. +62-274-580839,♥email: [email protected] Faculty of Agriculture, Gadjah Mada University. Jl. Flora Bulaksumur,Sleman 55281,Yogyakarta, Indonesia3UPTD Balai Pengembangan Perbenihan Tanaman Pangan danHortikultura, Ngipiksari, Kaliurang, Sleman, Yogyakarta, Indonesia

Cultivation of potato in mid elevation areas is challengingto fulfill the demand of seed potato which has increased intropical countries. An experiment was conducted with theaims to (i) evaluate the physiological mechanism inbreaking dormancy of potato tubers and (ii), to evaluate theeffect of paclobutrazol on growth and yield of severalpotato varieties grown in mid elevation areas. Four potatovarieties were used namely Granola-Pengalengan, Granola-Kopeng, Atlantik and Nadia. For dormancy breakage,potato seeds of 40-50 g were placed under cold (10 oC) ormild temperatur (25 oC) and moist condition for 4 weeks.Number of sprout and shoot length was measured, α-amylase activity was determined by the Ceralpha amylasemethod, and hormones content were determined usingHPLC. To analyze the effect of paclobutrazol on growthand yield of potato, all potato seed varieties were planted inthe mid level field of 700-800 m above sea level, withday/night temperature range of 28 oC to 31 oC/22 oC to 25oC. The soil was enriched with organic fertilizer (2,5 t/ha),SP36 (150 Kg/ha), urea (100 Kg/ha) and KCl (110 Kg/ha).Potato seeds were planted in a row with the distance of 30cm between each tuber. Four weeks after plantingpaclobutrazol at concentrations of 0 ppm (control), 12,5ppm, or 25 ppm was applied by spraying it to the plant andalso as a soil drench. On week 8th plants were sampled andchlorophyll content was determinedspectrophotometrically. Plant height, nitrate reductaseactivity, reducing sugar content, starch content, Vitamin Cand potassium content in the tubers were also evaluated.The results showed that all four varieties of potato cangrow relatively good on mid elevation areas having altitudeof about 700 m above sea level and produced tubers thatcan be used either for seeds or for consumption. Tubersprouting occurred 3 to 4 weeks after storage at 22-25oC.Potato tubers that were subjected to low temperature (10oC) had greater α-amylase activity compared to those seedpotatoes stored at room temperature, but sprouting in lowtemperature was delayed for about 5 to 6 weeks comparedto mild temperature (22-25 oC). In germinating tubers,cytokinin was detected in all tuber varieties used in thisexperiment. The content of cytokinin in sprouting tuber ofGranola-Pengalengan, Nadia and Atlantik was relativelysimilar, but for Granola-Kopeng the content of cytokinin

was only one fourth of the content in other varieties. Auxinwas only detected in tuber of Granola-pengalengan. Theoccurence of cytokinin in the tubers indicated that thishormone play a role in tuber development as well as tubersprouting. Paclobutrazol treatment reduced the average ofplant height; however, it tends to increase the total proteincontent. The number of new tubers, starch content, nitratereductase activity and potassium content increased bypaclobutrazol at 12.5 ppm or 25 ppm especially onGranola-Pengalengan variety. On the other hand, thecontent of vitamine C remained relatively similar in allvarieties of either the control or paclobutrazol treatments.The Atlantic variety produced greater size of new tuber butthe number of new tuber was less compared to othervarieties examined in this research. It is probably due to theflower formation which occured at the same time of tuberinduction, thus compiting for photo assimilates which thenlimited the formation of more tubers.

Dormancy breakage, growth, mid level area, paclobutrazol,potato

EO-02Introduced new forages species to sustainbiodiversity in coconut based farming

David A. Kaligis♥, Selvie D. Anis, Malcky M. TellengFaculty of Animal Husbandry,Sam Ratulangi University.Jl. Kampus UNSRAT Manado 95115, North Sulawesi, Indonesia. ♥email:[email protected]

In tropical region where the coconuts commodity as a backbone economy such as in Indonesia rearing cattle with localforages grown naturally underneath coconuts plantation is asystems widely practices. This paper is composed ofinformation of several years of our consecutives resultsresearch (five filed experiment separately). In coconutplantation native forages and natural vegetation can’tsurvive under heavy grazing due to lack of grazingmanagement practiced by farmers. This will lead toovergrazing, soil compaction and in some circumstancestriggering erosion, changing in botanical composition dueto invasive weeds, and disappearance of the plantingforages species. This disappearance is also probably due toother factors such as intolerance of those species in theshaded environment. However, this is not the case.Although shade tolerance species are planted, the pasturerun-down still occurs. To overcome this problem there aresome new approaches. Defoliation should based onbiogeochemical process consideration and should ensurethe health of the grass subject to cutting. Abundance ofCO2 due to climate change following by the increasing ofair temperature, the time of defoliation or grazing should befocused on counting the accumulation of heat unit ratherthan focusing on forage’s age. Finally, appropriate grazingmanagement should take into account, to ensure pasture torecover after grazing. It was concluded that interaction ofrotational grazing with grasses at 3.5 rd mature leaves and


the stocking rate at 2.31 AU gave the best pastureperformance, botanical compositions, daily gain of cattleand improved the yield of coconut nuts.

Biodiversity, coconut, farming, new forage species

EO-03Stability of rhizobacterial isolate in some formulasto increase growth and yield of soybean in the field

Trimurti Habazar♥, Yulmira Yanti, Zurai Resti, JulioEifelt RumbiakFaculty of Agriculture, Andalas University. Kampus Unand Limau Manih,Padang 25163, West Sumatra, Indonesia. Tel. +62-751-72773, Fax.: +62-751-72702, ♥email: [email protected]

Plant growth promoting rhizobacteria are a group ofbacteria, that actively colonize plant roots, induce theresistance of plant to pathogen, increase plant growth andyield. Our previous research in greenhouse experiment hadshowed, the stability of some formulas of rhizobacterialisolates from soybean root effective to increase growth andyield of soybean. The aim of this research was to get thebest carrier for formulation to maintain the effectivity ofrhizobacterial isolate on storage to increase plant growthand yield of soybean in the field. The experimental wasdesigned in complete randomized design where 16treatments and three replicates with three plants/treatmentfor each variety was taken. The treatments werecombination of material carrier for formulation ofrhizobacterial isolate (peat soil, tapioca flour and coconutwater + 1% palm oil) and time of storage of formula (0, 1,3, 5 and 7 weeks) and control. Every formula ofrhizobacterial isolate were inoculated on soybean seed asseed treatment. The parameter were observed include: theviability of rhizobacterial isolate in the formula duringstorage, growth development (germination rate, plantheight, number of leaves and twigs), generative phase (timeof flowering and pod) and yield (weight of seed) ofsoybean. The results showed that all formulas ofrhizobacterial isolate were able to suppress the bacterialpustule on soybean. The best combination of rhizobacterialformula to increase growth and yield of soybean were fourweeks storaged of cassava flour and peat soil compare thancontrol.

Field experiment, formulation, plant growth promotingrhizobacteria, soybean

EO-04The soil improvement potential of weeds inmarginal land

Anti Damayanti Hamdani1,♥, Eka Sulistiyowati2, ArifahKhusnuryani1

1Biology Department, Faculty of Science and Technology, State IslamicUniversity Sunan Kalijaga. Jl. Marsda Adisucipto No. 1 Sleman 55281,Yogyakarta, Indonesia. ♥email: [email protected] Education Program, Faculty of Science and Technology, StateIslamic University Sunan Kalijaga. Jl. Marsda Adisucipto No. 1 Sleman55281, Yogyakarta, Indonesia.

A farmed soil that has been allowed to lie idle and grow upto weeds is more productive when cultivated again than itdid before, because the presence of weeds building uporganic matter and chemical contents of soil. This studyaimed to assess the soil improvement potential ofChromolaena odorata, Mikania micrantha, and Synedrellanodiflora, also the best form of their practical applicationas green manures without fermentation in marginal land.Two forms of application, fresh and dried chopped, wereexamined in a pot experiment using soil from marginalland of Patuk, Gunung Kidul, and spinach (Amaranthustricolor L.) as the test crop. These were compared to thecontrol groups of pot without any soil amendments and onewith anorganic fertilizer. The pots were laid out inrandomized complete block with six replications.Laboratory analysis showed that manurial propertiesbetween the weeds were statistically similar. The highestorganic C (38.77%) and N (3.4%) were found in C.odorata, while M. micrantha contained the highest level ofP and K at 0.16 and 2.1%, respectively. Of the chemicalcontents considered, the content of all measured chemicalsin experimental soil had increased after incubated withchopped weeds for two weeks, of which significant risewas detected on available K2O from 3.33 to the average of46.33 ppm. The growth and yield of spinach resulted fromsoil treated with chopped weeds were superior than thatfrom untreated soil. However, there were no significantdifferences in the response of plants among the types ofweeds used. Rather, better growth and yield of spinachresulted from soil treated with dried chopped. Spinachyields were 12.89 and 46.29% higher when treated withdried chopped weeds than that with the fresh ones andanorganic fertilizer, respectively.

Chromolaena odorata, Mikania micrantha, soil fertility,Synedrella nodiflora

EO-05Identification the components of fatty acidcompounds on the seed of Leucaena leucocephalaas the potential sources of halal lecithin basematerials

Ika Nugraheni Ari MaartiwiFaculty of Science and Technology, State Islamic University SunanKalijaga. Jl. Marsda Adisucipto No. 1, Sleman 55281, Yogyakarta,Indonesia. email: [email protected]

Lecithin is one of the ingredients of food emulsifier thatcan be derived from animals, for example cows and pigs, orplants, such as soy. The extensive use of vegetable lecithinis still constrained by the availability of soy lecithin as thebase materials. Therefore, the obscurity of lecithin products


often creates doubts among Muslim to consume foodscontaining lecithin emulsifiers. This study was aimed toidentify the components of fatty acids compounds inLeucaena leucocephala L. seeds as the alternative sourceof lecithin base materials. Lecithin was extracted usingchloroform-methanol, followed by GCMS tests with soylecithin compound as standard. The results showed that theseeds of L. leucocephala containing lecithin fatty acidresidues of 0.37, 14.4, 64.9 and 3.26% meristat acid,palmitic acid, oleic acid and linoleic acid respectively.Therefore, L. leucocephala seeds can be used as a potentialsource of lecithin base materials.

Fatty acid, lecithin, Leucaena leucocephala, seed

EO-06Effect of genotype on sporofit formation fromprothallus mass of the golden chicken fern(Cibotium barometz) in vitro

Yupi Isnaini♥, Titien Ngatinem PraptosuwiryoCenter for Plant Conservation-Bogor Botanical Gardens, IndonesianInstitute of Sciences (LIPI). Jl. Ir. H.Juanda No. 13, P.O. Box 309 Bogor16003, West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥email:[email protected]

The golden chicken fern, Cibotium barometz (L.) J. Sm., isone of an Indonesian tree fern that has been included in theAppendix II of CITES since 1976. Its rhizomes and hairsare an important export commodity used as medicine, butthose parts of the plant still harvested from the wild. Inorder to conserve the species, Bogor Botanic Gardens hasbeen collect and propagate the plant true in vitro sporeculture. The best media for spore germination anddevelopment of the gametophytes of C. barometz were ½MS. However the growth from gametophyte stage to formsporophyte stage is very late. Previous study indicategenotype is one of the important factor that control thesporophyte formation. The aim of this study is to observesporophyte development of six genotype of C.barometz invitro. The spore of six genotype of C. barometz (Cb, Cb1,Cb2, Cb3, Cb4, and Cb5) were used as explant using halfstrength Murashige and Skoog (1/2 MS) basal media. Massprothallus of the spore culture were subculture in ½ MSbasal media and incubated in room culture to inducesporophyte. The result shows the high sporophytepercentage (100%) were Cb, Cb1 and Cb5 genotypes,however, the highest number of sporophyte (mean 11.1sporofit) was Cb5 genotype.

Cibotium barometz, genotype, medicianl fern, prothallusmass, sporophyte

EO-07Production of β-Mannanase on porang potato byBacillus subtilis LBF-005 and its potential formanno-oligosaccharide production

Yopi1,♥, Nanik Rahmani1, Alifah Mafatikhul Jannah1,Irfan Pebi Nugraha2, Roni Masri Ramadana2

1Research Centre for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, Jawa Barat, Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email:[email protected] of Biochemistry, Faculty of Mathematics and NaturalSciences, Bogor Agricultural University, Bogor 16980, West Java,Indonesia.

A marine bacterium of Bacillus subtilis was cultivated inASW medium contain NaCl and various mannan biomassas carbon source for mannanase production. The cells weregrown in sub merged fermentation to study the optimumcondition was evaluated. Endo-β-1, 4-mannanase is the keyenzymes of the mannolytic system, which randomlyhydrolyzing the β-1, 4-linkages within the mannanbackbone releasing mannooligosaccharides of variouslengths. The maximum enzyme activity was obtained withporang potato as a substrate with concentration 1%, pHmedium 8, and incubation temperature 50oC with anenzyme activity of 37. 7 U/mL. The mannooligo-saccharides product released by crude mananase frompotato porang were manose (M1), manobiose (M2),manotriose (M3), manotetraose (M4), and manopentaose(M5).

Bacillus subtilis, β-mannanase, mannan biomass,mannooligosaccharides, porang potato

EO-08Energy pathway in soil nematode food web underdifferent cropping pattern of maize and legumescrop

Laode Muhammad Harjoni Kilowasid1,♥, LaodeSabarudin1, Lisnawati1, Hasbullah Syaf1, Gusti RaySadimantara1, Syamsu Alam1, Namriah1, Mardin2

1Agrotechnology Department, Faculty of Agricultural, University of HaluOleo. Jl. HEA. Mokodompit, Kendari 93231, Southeast Sulawesi,Indonesia. Tel.: +62-401-3191692, Fax: +62-401-3191692, ♥email:[email protected] Department, Faculty of Agricultural, University of HaluOleo, Jl. HEA. Mokodompit,, Kendari 93231, Southeast Sulawesi,Indonesia

Soil nematodes occupied amount trophic levels and theiracquired carbon and nutrient from three energy channels inthe soil food web. The dynamics of the energy channelscould be affected by the functional identity and stage ofplant development. Objectives of this study is to analyzethe dynamics of the energy channels in soil food web usingsoil nematode trophic group during the development ofmaize and legume crops at different planting patterns.Seven cropping patterns, mainly the monoculture of corn,peanuts, mung beans, and soybean, and corn intercroppingwith groundnuts, with mung beans and with soybean wastested. Soil nematodes at the phase of flowering, fruitingand harvesting crops were sampled. The results found outthe bacterivore nematode and herbivore nematode duringthe development of corn and legumes crop in all cropping


pattern, while the fungivore nematode group was found outin the intercropping of maize with soybean. Relativebiomass of nematode herbivore increased in line with thedevelopment of the plants. At each stage of plantdevelopment for all cropping patterns, the relativebacterivore biomass higher than relative herbivore biomass.It was concluded that the process of decomposition andnutrient cycling of soil organic matter dominated bybacteria energy pathway and second dominant is energypathway of root herbivores nematodes during thedevelopment of maize, soybeans, peanuts, and mung beanseither in monoculture or intercropping.

Bacterivore, biomass, channel, dominant, herbivore

EO-09Morphological variations and sex expression ofgametophyte of Cibotium barometz under in vitroconditions

Titien Ngatinem Praptosuwiryo♥, Yupi IsnainiCenter for Plant Conservation-Bogor Botanical Gardens, IndonesianInstitute of Sciences (LIPI). Jl. Ir. H.Juanda No. 13, P.O. Box 309 Bogor16003, West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥email:[email protected]

Characters of gametophytes were shown to bephylogenetically significant. Study on fern gametophyteshas become essential to complete morphological,ecological, reproductive and distribution knowledge. Thepurpose of this study were to observe morphologicalvariation and sex expression of mature gametophyte ofCibotium barometz (L.) J. Sm. under in vitro conditions.Spores of six genotypes of C. barometz from Sumatra,Indonesia, were sown on sown in half-strength Murashige& Skoog (½MS) basal medium. After 11 weeksgermination of spores, prothalli were obtained. The protalliwere consisted of spatulate stage and young heart-shapedstage. Prothalli were subcultured on half-MS medium alongwith sugar (30 g/L) and Naphthelenacetic acid (NAA, 0.5mg/L) where they multiplied successfully. After 8 monthssubcultured of prothalli, gametophyte were observed bychoosing 100 gametophytes randomly of each genotype.Eight morphological types of adult gametophytes wererecorded: (i) branching filament (male), (ii) ribbon-likeshape (male), (iii) spatulate shape (male), (iv) heart shape(male, female, bisexual), (v) gemmiferous heart-shaped(asexual), (vi) long heart-shaped (male); (vii) gemmiferouslong heart-shape (asexual, and (viii) gemmiferous irregularshape (asexual). It is presumed that there is a correlationbetween gametophyte size, shape and sex expression, andto be related to the population density, which significanntlyaffects the sexual expression of gametophytes. It is alsoshowed that gametophyte morphology is simply “tooplastic” to be used in suporting species delimitation in fernsif the prothalli to be cultured in a heavy population densityunder in vitro conditions.

Cibotium barometz, gametophyte, sex expression

EO-10Pathogenicity of entomopathogenic fungusMetarhizium spp. against predators Menochilussexmaculatus Fabricius (Coleoptera: Coccinelidae)

Trizelia♥, Munzir Busniah, Agung PermadiDepartment of Plant Protection, Faculty of Agriculture, AndalasUniversity. Kampus Unand Limau Manih, Padang 25163, West Sumatra,Indonesia. Tel. +62-751-72773, Fax.: +62-751-72702, ♥email:[email protected]

Metarhizium spp. is entomopathogenic fungus which haswide host range. Metarhizium spp. can not only infectinsect pests, but can also infect beneficial insects such aspredators. The pathogenicity of four isolates ofMetarhizium spp. was investigated against Menochilussexmaculatus predator. Isolates of Metarhizium spp. wereisolated from rhizosphere of cacao,cabbage, chili andrubber crop. The experiment was conducted by treating thefourth instar larvae with an appropriate conidial suspensionof 108 conidia/mL. The results showed that all isolatesMetarhizium spp. were pathogenic toward beetle predatorM. sexmaculatus. Mortality of larvae within 7 days afteraplication of conidial suspension varied between 27.50 to67.50% and there were statistically significant differencesamongs the tested isolates. Metarhizium spp. had also asignificant affect in reducing pupation and adult emergenceof M. sexmaculatus to below 30% and 3%, respectively.These studies indicate that entomopathogenic fungusMetarhizium spp. was pathogenic to beetle predators,Menochilus sexmaculatus.

Beetle predators, entomopathogenic fungus, Menochilussexmaculatus, Metarhizium

EO-11Antioxidant potency of etanolic extracts of callusdeveloped from carrot and tomato

Rumiyati1,♥, Sismindari1, Endang Semiarti2, SekarGaluh2, Christine Alfiani Hana2

1Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, Indonesia.♥email: [email protected] of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.

Carrot (Daucus carota L.) and tomato (Solanumlycopersicum L.) are high potential source of antioxidantcompounds such as phenolics and carotenoids. Thecompounds are known to be able to protect human bodycells and tissues from free radical. Callus cell culture fromorgans of plants as explants can produce secondarymetabolite as source of antioxidants.This study was aimedto determine the potency of antioxidant activity of callusdeveloped from the plant organ of carrot and tomato onMurashige and Skoog (MS) medium. Murashige and Skoog(MS) medium used in this study was variated at sucroseconcentration of 30 g/L and 40 g/L, whereas the variationof 2,4-D concentrations was (0, 1, 2, 4 and 8) ppm. The


results showed that callus of the samples were able to growat 5-7 days after planting from different part of organplants. The callus was then sub cultured and extractedusing etanol. The etanolic extracts of callus of carrot andtomato were measured on their antioxidant activity usingDPPH method. The IC50 etanolic extract of carrot was1751.29 ± 4.00 mg/mL while etanolic extract of tomatowas 620.56 ± 32.36 mg/mL. Cuersetin used as standard hasIC50 value of 2,85mg/mL.

Antioxidant, carrot, callus, tomato

EO-12Study of gamma irradiation on the creating ofmutant of local rice mentik susu variety

Ahmad Yunus1,♥, Samanhudi1, Parjanto1, SusiloHambeg Poromarto1, Dyan Farisa2

1 Faculty of Agriculture, Sebelas Maret University. Jl. Ir. Sutami 36ASurakarta 57126, Central Java, Indonesia. Tel./Fax. +62-271-637457,♥email: [email protected] Private Agriculture Company, Pati, Central Java, Indonesia

Rice (Oryza sativa L.) is the most important food crop inIndonesia. The problem is the unavailability of riceproducing high quality and hight yield. The aim of researchwas to obtain rice mutant that producing higher yield andhaving short-lived. This study used gamma irradiation with5 level which were: 100 gray (R1), 200 gray (R2), 300 gray(R3), 400 gray (R4) and 500 gray (R5). The experimentwas conducted on agricultural land in the villageMojosongo, Boyolali District, Central Java in January toMay 2014. Three plants which considered as mutants hasbeen detected. The three plants producing 95 tiller morethan control and having shorter lived 26 days less thancontrol. Further research needs to be done for nexgenerations M2, M3, and M5 to observe the stability ofmutant due to gamma irradiation to obtain the maximumresults and production of rice mutant.

Local variety, Gamma irradiation, Mentik susu

EP-01Expression of Wnt4 gene in the Swiss Webstermice uterus as candidate antigen for wildlifeimmunocontraception method

Agung Janika Sitasiwi1,♥, Wayan Tunas Artama2,Agung Budiyanto3, Edi Dharmana4

1Biology Department, Faculty of Science and Technology, University ofDiponegoro, Jl. Prof Soedarto SH, Tembalang Campus, Semarang 50275,Central Java, Indonesia. ♥email: [email protected] Division, Faculty of Veterinary Medicine, Gadjah MadaUniversity. Sleman 55281, Yogyakarta, Indonesia.3Reproduction Division, Faculty of Veterinary Medicine, Gadjah MadaUniversity. Sleman 55281, Yogyakarta, Indonesia.4Parasitology Division, Faculty of Medicine, University of Diponegoro.Semarang 50275, Central Java, Indonesia.

Immunocontraception is a procedure to altere theconception in animal using immune system. The concept ofimmunocontraception can be delivered firstly by isolatingand identifying protein or gene that significantly plays arole in one of the reproductive processes to be used asantigen. Some peptides have been developed as source ofantigen, however, it does not give a satisfactory result.Wnt4 gene is a regulator of mammalian embryoimplantation process could be develops as source of theantigen candidates in immunocontraception. The aim ofthis study is to find the expression of Wnt4 in SwissWebster mouse uterine. Uterine were isolated fromimplantation site of mice at seven days gestational age,fixed in 4% paraformaldehyde, embedded in paraffin, andthen sectioned at 7 µm for immunohistochemistry. Thedetection of Wnt4 gene expression was run byimmunohistochemistry procedure using antibody anti-Wnt4(Santa Cruz Ltd.). The result of this study shows that Wnt4gene expression gives positive reaction to antibody antiWnt4 almost of the uterine tissues. This study indicates thatWnt4 gene were present in the mouse uterus duringimplantation period of pregnancy. Overall the results ofthis study demonstrate that Wnt4 gene from Swiss Webstermice will be very beneficial as an antigen source inregulating fertility of wildlife.

Antigen, immunohistochemistry, Wnt4

EP-02The influence of the media Limboto lake sedimenton the growth of maize were inoculated arbuscularmycorrhizal

Novri Youla Kandowangko♥, Jusna Ahmad, Fujiawaty,Asni Santi PatilimaDepartment of Biology, Faculty of Mathematics and Natural Sciences,Gorontalo State University. Jl. Jendral Sudriman No. 6, Kota Gorontalo96128, Province Gorontalo, Indonesia. Tel. +62-435-821125, ♥email:[email protected]

Lake Limboto now be categorized as critical lake called theSea are broad and deep decline. In 1932, the area of LakeLimboto of 7,000 hectares and a depth of 30 m, in 1962,was reduced to 4,250 ha breadth and depth of the lake is 10m. In 2012, the area of the lake is estimated to be only3,000 ha and depth of about 2 m. The thickness of thesediment/sludge in Lake Limboto estimated 3-5 m in theeast, from 5.8 to 6.4 m along the north-west, from 8.8 to10.2 m along the south side and 12.4 m in the middle of thelake. One effort to reduce the thickness of the sediments ofLake Limboto (Sedalim) is to dredge the sediment backinto the media and use it to grow plants. However Sedalim,besides containing many nutrients carried by water runoffthat comes from agricultural land, also contain toxicsubstances derived from household waste and smallindustry located around Lake Limboto. This study aims todetermine the effect of arbuscular mycorrhiza on thegrowth of corn plants (Zea mays L.) grown on media whichinoculated arbuscular mycorrhizal Sedalim. Research


carried out by using the experimental method DesignGroup Random Design (RAK) consisted of 4 treatmentswere 0, 3.75, 7.5, 11.25 g/LT arbuscular mycorrhizalinoculants, with four replications. Analysis of data usinganalysis of variance and analysis of plant growth such asthe rate of plant growth (CGR) and Net assimilation rate(NAR). The results showed that administration ofarbuscular mycorrhizal inoculant effect on the growth ofcorn plants grown on media Sedalim. It looks at indicatorssuch as plant growth and NAR values CGR corn crop.NAR value increased in line with increasing doses ofarbuscular mycorrhizal given, while the average valuedecreased from age CGR plants 20-30 days after planting.

arbuscular mycorrhiza, corn, sludge, CGR, NAR

EP-03Effects of compost type and rootstock length ongrowth response of fruit seedlings and vegetableplants in the nursery

Dody Priadi♥, Enung Sri MulyaningsihResearch Center for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected]

Three species of fruit plant i.e. sweet star fruit (Averrhoacarambola L.), durian (Durio zibethinus Murr.) and guava(Psidium guajava L.) were propagated by either grafting orbudding technique. The grafted plants were grown on themedia containing various type of compost i.e. grasscompost (K-1), spent compost of paddy straw mushroom(K-2), and spent compost of oyster mushroom (K-3) incombination with rootstock length i.e. 45-55 cm (TB-1),65-75 cm (TB-2) and 75-90 cm (TB-3). The same compostwas also used as soil amendments for growing vegetablesplants i.e tomato (Solanum lycopersicum L.) and kangkung(Ipomoea reptans Poir) in the screen house. Result showedthat the highest survival of grafted sweet star fruit (71.11%and 71.56%) was obtained from TB-3 grown on mediacontaining K-1 by grafting and budding techniquerespectively. The highest survival of grafted durian(68.89%) was obtained from TB-1 by grafting technique.Budding technique seems to be not appropriate for guavapropagation which was indicated by 0% of survival. Alltypes of compost applied to grafted fruit plant were notdifferent in each growth parameters observed. Applicationof compost on tomato plants as fruit vegetable showed thatspent compost of oyster mushroom of 3 kg resulted in thehighest of fruit fresh weight, length and diameter.Meanwhile, the same volume and type of compost appliedto kangkung as leaf vegetable resulted in the highest ofplant height, total leaves and total biomass.

vegetative propagation, fruit plant, vegetable plant, compost,growth parameter

EP-04Correlation of pod characteristics with pod borrerEtiella zinckenella Treitschke preference

Kurnia Paramita S.♥, Suharsono, M. Muchlish AdieIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Pod borer Etiella zinckenella is one of the major pests insoybean. Physical pod character influenced host plantpreference. The aim of the researchh was to determine thecorrelation of pod characters with pod borer preference. Asmany as 16 soybean genotypes were evaluated. Intensity ofpod injury, intensity of seed injury, stalks length; trichomedensity and trichome position were observed in this study.The result showed that low injury intensity was found onerect and oblique trichome position. Furthermore, stalklength did not correlate with injury intensity. P6, P8, andP9 were less preference to pod borer attack. Pod injuryintensity was positively correlate with seed injury intensity(r=0,85).

Physical characteristic, pod borer, pod soybean

EP-05Effect of five host plants on the growth anddevelopment of armyworm, Spodoptera litura

Marida Santi Yudha Ika Bayu♥, Ayda KrisnawatiIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:[email protected]

Spodoptera litura is the important pest on soybean inIndonesia. The objective of this study was to determine theinfluence of different host plant on the growth anddevelopment of S. litura. This research was conducted inLaboratory of Entomology, Indonesian Legume and TuberCrops Research Institute, Malang, East Java on March-April 2014 using randomized complete design, fivetreatments and 30 replicates. The treatments were Glycinemax, Jatropha curcas, Ricinus communis, Ipomoeaaquatica, and Amaranthus spp. One leaf of each host plantwas entered into test tube and was infested by one larva ofS. litura 0 days after emergence. The result showed thatfeeds significantly affected the body size, survival rate,reproduction, and longevity of S. litura. The longest andheaviest larva were found on I. aquatica, 28,5 mm and 0,42g respectively. The longest, widest, and heaviest pupa werefound on I. aquatica, 19,3 mm; 5,0 mm; 0,36 grespectively. Survival rate was highest on soybean (29individuals), followed by R. communis (26 individuals).Furthermore, lifetime fecundity was high on I. aquatica (9egg/female). In conclusion, I. aquatica and R. communisleaves were found to be preferred for S. litura life so it canbe used as feed for mass rearing of S. litura.


Development, host plant, growth, reproductive system,Spodoptera litura

EP-06Mycorrhizal population on various croppingpatterns on sandy soil in dryland area of NorthLombok, Indonesia

Wahyu Astiko♥, Muhammad Taufik Fauzi, SukartonoFaculty of Agriculture, University of Mataram. Jl. Majapahit No. 62,Selaparang, Kota Mataram 83124, West Nusa Tenggara, Indonesia. Tel.:+62-370-621435, ♥email: [email protected]

Inoculation of arbuscular mycorrhizal fungi (AMF) onmaize in sandy soil is expected to have positiveimplications to the improvement of AMF population andnutrient uptake. However, how much increases in the AMFpopulation and nutrient uptake in the second cycle of acertain cropping patterns commonly cultivated by thefarmers after growing their corn crop has not beenrevealed. Since different cropping patterns would indicatedifferent increases in the populations of AMF and nutrientuptake. This study aimed to determine the population AMFand nutrient uptake on the second cropping cycle of corn-based cropping patterns which utilized indigenousmycorrhizal fungi on sandy soil in dryland area of NorthLombok, West Nusa Tenggara. The experiment wasconducted at the Akar-Akar Village in Bayan Sub-districtof North Lombok District, in a Randomized Block Design,with 4 replications and 6 treatments of cropping cycles (P0= corn-soybean as a control, in which the corn plants werenot inoculated with AMF; P1 = corn-soybean, P2 = corn-peanut, P3 = corn-upland rice, P4 = corn-sorghum, and P5= corn-corn, in which the first cycle corn plants wereinoculated with AMF). Results indicated that themycorrhizal populations (spore number and infectionpercentage) were highest in the second cycle sorghum,achieving 335% and 226% respectively, which weresignificantly higher than those in the control. Increaseduptake of N, P, K and Ca the sorghum plants at 60 DAS ofthe second cropping cycle reached 200%; 550%; 120% and490% higher than in the control. The soil used in thisexperiment is rough-textured (sandy loam), so it isrelatively low in water holding capacity and high porosity.

Corn, cropping pattern, dryland, mycorrhizal

EP-07Isolation and characterization of mannanase,xylanase and cellulase from marine bacteriaBacillus sp.

Yopi♥, Apridah Cameliawati Djohan, Nanik Rahmani,Alifah Mafatikhul JannahResearch Center for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor

16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected].

Isolation, identification and characterization of mannanase,xylanase and cellulase producing indigenous marinebacteria have been conducted from total 20 isolates. Basedon 16S rDNA sequence analysis, three potential isolatesidentified as Bacillus subtilis (M8), Bacillus tequilensis(X4) and Bacillus cereus (C9). The potential strains M8,X4 and C9 can produce mannanase, xylanase and cellulaseactivities such as 9.5 U/mL; 0.36 U/mL;0.56 U/mL withoptimum pH and temperature 6.0;50oC, 5.5;70 oC and 8;50oC respectively. Based on the TLC analysis, mannanasefrom M8 and xylanase from X4 has potential to hydrolyzedmannan and xylan for producing oligosaccharides with sizearound tri-hexasaccharides as a main product.

Bacillus, cellulase, hemicellulase, marine bacteria,oligosaccharide

EP-08In vitro embryo development of a wild orchidDendrobium phalaenopsis as an efficient methodfor ex situ conservation

Nintya Setiari1,♥, Aziz Purwantoro2,♥♥, SukartiMoeljopawiro1, Endang Semiarti1,♥♥ ♥

1 Graduate Study Program of Biology, Faculty of Biology, Gadjah MadaUniversity. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta,Indonesia. Tel./fax. +62-274-580839, ♥email: [email protected];♥♥♥ [email protected] Faculty of Agriculture, Gadjah Mada University. Jl. Flora Bulaksumur,Sleman 55281, Yogyakarta, Indonesia. Tel./fax: +62-274-551228,♥♥email: [email protected]

Dendrobium phalaenopsis Fitzg is an endemic orchid fromLarat Island in the eastern Indonesia at Maluku region withbeautiful purple butterfly-shaped of flowers. The existenceof this orchids is endangered in their original habitat,therefore ex situ conservation is needed. D. phalaenopsisorchid seeds are microscopic sized with no endosperm, soin vitro culture on artificial medium is the best method forseed germination. The objective of this study is to analysethe stages of embryo development of this orchid during invitro condition and to confirm whether the addition oforganic supplement such as coconut water (CW) andpeptone (P) in medium can improve the quality andquantity of embryogenesis or not. The methods wereperformed using 2.5 months old orchid pods. Orchid seedswere sown on Vacin and Went (VW) basic medium andVW with addition of 10% of CW and 2 g/L P. The growthand development of embryos were analysedmorphologically and anatomically every weeks. The resultshowed that there were 6 stages of embryo developmentfrom embryo to protocorm (tuber-like developing orchidembryo) up to shoot formation. Eight weeks after seedsowing, about 67.30% seed germinated on VW basicmedium, but it reached 94.42% seeds germination onVW+CW+P medium. This data indicate that the addition of10% of CW and 2 g/L of P in VW basic medium accelerate


the growth and development of D. phalaenopsis orchidsembryos in the in vitro condition as the best method forproducing plantlets.

Coconut water, Dendrobium phalaenopsis, embryogenesis,peptone, Vacin and Went medium

EP-09Construction of 35S::PaFT1::GFP containing Tiplasmid in Agrobacterium tumefaciens EHA101 forgenetic transformation in plant

Sri Wahyuningsih1,2,♥, Muhammad Dylan Lawrie1, BudiSetiadi Daryono1, Sukarti Moeljopawiro1, EndangSemiarti1

1Graduate Study Program of Biology, Faculty of Biology, Gadjah MadaUniversity. Jl. Teknika Selatan, Sekip Utara, Sleman 55281, Yogyakarta,Indonesia.Tel/Fax. +62-274-580839, ♥email: [email protected] address: Department of Biology, University of Lampung,Lampung, Sumatra, Indonesia

Flowering in plants occurs due to the transtition fromvegetative phase to generative phase. Flowering locus T(FT) is one of the genes that activates flowering inArabidopsis. PaFT1 gene is one of FT homologous geneisolated from P. aphrodite. With analogous to the modelplant Arabidopsis, over expression of PaFT1 gene isexpected can be used to accelerate flowering in plants. Theobjective of this study is to construct a useful Ti plasmid asa recombinant plasmid that harbour T-DNA which containsfunctional PaFT1 gene and GFP reporter gene that willallow easy and effective screening for transformant plants.The method was carried out by cutting the 35S::GFPfragment (350 bp in size) from pSK35S::GFP plasmidusing EcoRI restriction enzyme digestion. The 35S::GFPfragments were running in 0.8% agarose gelelectrophoresis, then cut of from the gel and following thegel extraction method. The resulting fragment of 35S::GFPwere then examined using PCR with specific primers forGFP and HPT. The pGAS 101 plasmid that containedPaFT1 gene was opened using EcoRI restriction enzymes,then inserted with the 35S::GFP fragments, ligated it withLigation High-DNA ligase enzymes. The results showedthat the fragment of 35S::GFP from pGAS::GFP plasmidwas successfully inserted into pGAS101, hereafter it termsas pGAS 101-GFP. The recombinant plasmid ofpGAS101::GFP then was transfered into Escherichia colistrain DH5α. After checking the structure of pGAS::GFPplasmid, the plasmid was isolated from DH5α andtransfered into A. tumefaciens strain EHA101.Fluorescence microscope analysis showed that the GFPexpress green fluorescence under uv light, indicating thatthe plasmid pGAS 101::GFP was a recombinant Ti-plasmidthat contains the recombinant T-DNA with35S::PaFT::GFP. The recombinant p35S::PaFT1::GFP/A.tumefaciens strain EHA101 is ready to be used for genetictransformation to accelerate flowering in plants.

A. tumefaciens strain EHA101, E. coli strain DH5α,flowering in plant, GFP, PaFT1

EP-10Green pod culture of variegated-Phalaenopsis"Sogo Vivien" orchid

Exsyupransia Mursyanti 1,♥, Aziz-Purwantoro1,2,Sukarti Moeljopawiro1,3,Endang Semiarti 1,3,♥

1Biotechnology Study Program, Graduate School, Gadjah MadaUniversity, Yogyakarta, Indonesia. ♥email: [email protected] Faculty of Agriculture, Gadjah Mada University,Jl. Flora Bulaksumur,Sleman 55281, Yogyakarta, Indonesia.3 Faculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia.Tel/Fax. +62-274-580839,♥♥email: [email protected]

Phalaenopsis Sogo Vivien are mini attractive orchidhybrids, which some of their plants exhibit beautifulvariegated pattern of leaves. Green pod culture was done inorder to know the development of germinated seed, the bestmedium for embryo growth, and the phenotype of progeny(F1). This study used seed as explants which were obtainedfrom the 2.5 month old green pod orchids as the result ofself pollinationof variegated- Phalaenopsis Sogo Vivienand from backcross with the parents, P. amabilis. The VW,NP and MS media were used for seed plantation fromselfing, while NP medium was used for seeds plantationfrom the backcrosses. The growth of Phalaenopsis SogoVivien embryo in NP medium showed 6 differentdevelopmental phases, and the presence of Shoot ApicalMeristem (SAM) followed by the formation of increasinglydense absorbing hair (phase 6) was observed in 4 weekafter sowing (WAS). The use of NP medium showed thebest growth and development of protocorms. Thepercentages of the late phase (phase 5 and 6) and the size ofprotocorm growing on NP medium showed larger than thatin VW or MS medium. The emerged plantlet from selfingmostly had non-variegated leaves phenotype and only one(out of 6450 seeds) showed variegated leaves. All plantletsobtained from the backcross showed normal phenotypewith no-variegated leaves. These phenomenon shows thatvariegated leaves phenotype is a very low frequency ofmutation that can only be mass produced through in vitroculture using vegetative parts instead of seed (generative).Green pod culture was effectively used for quick detectionof plant phenotype without having to wait too long until thefruit becomes ripe.

Green pod culture, protocorm, Phalaenopsis Sogo Vivien,variegated-leaves


EP-11Study of cross inoculation of Rhizobium tropici(DCM) isolated from Acacia mangium incombination with other potential soil microbes ontheir ability to support the growth of soybean

Sylvia Lekatompessy♥, Liseu Nurjanah, HarmastiniSukimanResearch Center for Biotechnology, Indonesian Institute of Sciences(LIPI). Cibinong Science Center, Jl. Raya Bogor Km. 46 Cibinong-Bogor16911, West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588, ♥email: [email protected]

Rhizobia bacteria have been well known as nitrogen fixingbacteria which could promote the growth of soybeanplants. The bacteria could established a symbiosis livingwith crops and trees legume. The successful symbiosisliving is normally identified by the specific compatibilitiesbetween the bacteria and host plants. However, there is achance that the bacteria which was isolated from treeslegumes could also infect and be compatible in developingthe symbiosis living with crops plant such as soybean. Thisstudy aims to confirm that Rhizobium bacteria isolatedfrom tree legume, Acacia mangium, could successfullydevelop the symbiosis living with soybean. In addition, thisresearch also study the possibility of Rhizobium bacteria inworking with other potential soil microbes such asmycorrhizae and others potential microbes on supportingthe growth of soybean. Green house experiment showedthat Rhizobium tropici combined with other potential soilmicrobes can significantly support the growth of soybean.This is shown with the increase of upper plant biomass andnumber of pods. This research finally opens the possibilityof using biodiversity of nitrogen fixing microbes to bepacked as an update quality biofertilizer for soybean.

Biofertilizer, mycorrhizae, Rhizobium tropici

EP-12Fecundity performance of nile tilapias (Osteochilushasselti) from different districts in West Java

Rita Rostika, Yuli Andriani, JuniantoFaculty of Fisheries and Marine Sciences, Padjadjaran University. Jl. RayaBandung-Sumedang Km 21, Jatinangor, Sumedang 45363, West Java,Indonesia. Tel.: +62-22-87701519, Fax.: +62-22-87701518, email:[email protected]

A study on the reproductional aspect of nile tilapias(Osteochilus hasselti) has been conducted from October2015 to March 2016 that includes elements of body length,weight, gonad maturity level (GML), fecundity, anddiameter of the eggs. Samples for the study are collectedfrom farming activities in Districts in West Java, such asCianjur, Tasikmalaya and Kuningan, that include 33 fish.The observed parameters are the correlation betweenweight and fecundity, correlation between body length andfecundity, GML and the diameter of the eggs. The methodused for this research is explorative approach and the dataare analyzed with descriptive approach. The results fromthe observation show that the relation between the weightand fecundity in Cianjur District is y= e0.024x, while thebody length and fecundity is y=e0.413x. In TasikmalayaDistrict it is found that the correlation between the weightand fecundity is y= e0.4877x, while the correlationbetween the length of the fish and fecundity is y=e0.0724x.In Kuningan District it’s found that the correlation betweenthe weight and fecundity is y= e0.2902x, while thecorrelation between the length of the fish and fecundity isy=e0.6859x. The dominant gonad maturity level of Niletilapias in Cianjur is GML 4 (50%), Tasikmalaya is GML 6(100%), and Kuningan is GML 5 (55,56%).

Eggs diameter, fecundity, Osteochilus hasselti, West Java

international conference on biodiversity - smujo.idsmujo.id/s/2016/jogja/images/a0302aaall.pdf ·...

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