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2. Diagnostic Efficacy of Pleural Fluid Cytology in Feline Pleural Effusion

3. The Study on Hematology and Immune Level to Newcastle Disease Virus in

Betong (KU-Line) and Pradu Hang Dam Chicken

4. The Study of Antibody Titer of Newcastle Disease in White leghorn Chicken

5. Isolation of Peripheral Blood Mononuclear cell and Toll-like Receptor 4

Expression in White leghorn and Betong (KU-Line) Chicken

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Published by

· .

Japan Int'l Conference Proceedings

of 11th International Conference on Chemical, Agricultural, Biological and

Environmental Sciences (CARES-18)

8th International Conference on Computer, Electrical and Electronics Engineering & Technology (ICEEET-18)

11th International Conference on Biological, Biomedical, Medical and Pharmaceutical Sciences (Cellular and Molecular Approach) (BBMPS-18)

&

9th International Conference on Urban Planning, Architecture, Civil and Environment Engineering (UPA CEE-18)

April 17-18, 2018

Kyoto (Japan)

Editors: Prof. Kazuaki l\'Iaeda

Prof. Heecheul Kim

Prof. Dr. Elvis Fosso-Kankeu

ISBN 978-93-84422-85-1

Organized By:

Program Committee ICABES-18) Prof. Dr. Bulent Topcuoglu Environmental Protection and Control. Akdeniz University, Turkey

Prof. Rahim Ahmadi, Islamic Azad University, Iran

Prof. Dr. Takatoshi Ueno, Institute of Biological Control, Faculty of Agriculture, Kyushu University,

Fukuoka, Japan

Prof. Samia M. Sanad, Zoology Department, Faculty of Science, Zagazig University, Egypt

Dr. Md. Aminur Rahman, Institute of Bioscience, Universiti Putra Malaysia (UPM), Selangor Darul

Ehsan, Malaysia

Prof. Dr. Jose Manuel G6mez-Sober6n Superior School of BuildingfTechnical University of

Catalonia/Spain

Prof. Olker Ogutveren, Anadolu University, Turkey

Assoc. Prof. Mariam Gaidamashvili, Department of Biology, Faculty of Exact and Natural Sciences,

Iv. Javak~lishvili Tbilisi State University, Georgia

Assoc. Prof. Dr. AVARVAREI Bogdan-Vlad, "Ion lonescu de la Brad" University of Agricultural

Sciences and Veterinary Medicine from lasi, Romania

Prof. Dr AIT SARKA Essaid, Department of Biology and Biochemistry, Reims University, Unite de

Recherche Vignes et Vins de Champagne, France

Prof. Wanida Jinsart, Chulalongkorn University, Bangkok, Thailand

Prof. Youcef NECIB, Universite Constantine1, Algeria

Prof. Seung-Hoon Han, Chonnam National University, Korea

Prof. Nahed Mohamed Mohamed Ismail, Theodor Bilharz Research Institute (TBRI), Egypt

Prof. Salah Rhouat, Department of Chemisity, Faculty of Exact Sciences, University of constantine1,

Algeria

Assoc Prof. Dr. Moetaz El-Hawary, Civil Engineering Department, Kuwait University, Kuwait

Prof. Vinod Jagannathrao Mokale, University Institute of Chemical Technology, North Maharashtra

University, Jalgaon, India

Assoc. Prof. Dr. Mohammad Firuz Ramli, Faculty of Environmental Studies, Universiti Putra

Malaysia, Malaysia

Prof. Sawsan Sajid At-Jubori, AI -Mustansiriya University, Iraq

Assoc. Prof. Reyhan Erdogan, Akdeniz University, Antalya, Turkey

Prof. Dr. Saber Mohamed Abd-Allah, Theriogenology Department, Faculty of Veterinary Medicine,

Beni-Suef University, Egypt

Assoc. Prof. Dr. Auwallbrahim Magashi, Kano University of Science and technology, Wudil, Nigeria

Assoc. Prof. Dr. Yalcin Kaya, Trakya University Engineering Faculty, Turkey

Dr. Danladi M. Umar, Department of Biological Sciences, Gombe State University, Nigeria

Dr. Uun Yanuhar, University of Brawijaya, Indonesia

Assoc. Prof. Dr. Alaa Kareem Niamah, Food Science Department, Basrah University, Iraq

Dr. Christopher QUIRK, Dermatology Specialist Group, Australia

.. , , .

Program Committee Cont'd (CABES-18) Dr. Kazi Parvez Fattah, Department of Civil Engineering, American University of Sharjah, Sha~ah,

United Arab Emirates

Dr. Chan tara Thevy Ratnam, Malaysian Nuclear Agency (Nuclear Malaysia), Malaysia

Assist. Prof. Dr. Aynur DEMIR, Aksaray University. Department of Environmental Protection

Technologies. Aksaray, Turkey

Assoc. Prof. Dr. Rajabali Ebrahimi, Azad University of Takestan, Iran

Assoc. Prof. Dr. Isyaku Ibrahim Indabawa, Bayero University Kano, Nigeria

Assoc. Prof. Dr Amir Hossein Hamidian, Department of Environment, University of Tehran, Iran

Assoc. Prof. Dr. Manal Eid, Suez Canal University, Egypt

Prof. Dr. Noureddine HASSINI, University of Gran 1, Algeria

Prof. Dr. Emhemmed Abdullah Alhibshi, Chemistry Department, Faculty of Science. Aljabel Algharbi

University, Libya

Prof Dr. Maha Hady Ali, Cairo University, Egypt

Prof. Dr. Helal Ragab Moussa, Atomic Energy Authority, Egypt

Prof. Dr Usama Mohamed, Civil Department, Faculty of Engineering, MINIA University, Egypt

Prof Dr. Laila Montaser, Menoufia University, College of medicine, Egypt

Dr Salem Elwahaishi, Misurata University, Libya

Assoc. Prof. Dr. Shweta Rana, FLAME University, Pune, India

Dr. Salah Abdel Rahman Salih, University of Alneelain, Sudan

Dr. Nyoman Puspa ASri, Wr. Supratman University, Indonesia

Dr. Safiah Yusmah Muhammad Yusoff, Department of Geography, Faculty of Arts and Social

Sciences, University Malaya, Kuala Lumpur Malaysia

Dr. Deena Elsori, Abu Dhabi University, UAE

Assit. Prof Zaher Karima, Multiphase Polymeric Materials Laboratory, University Setif. Algeria

Dr. Altayeb Elazomi. Faculty of Medical Technology, Zawia University, Zawia Libya

Assrt. Prof Zeki Unal YOmun. Namlk Kemal University. C;orlu Engineering Faculty. Department of

Environmental Engineering, Turkey

Prof. Abid-UIRazzaq I Abu-Tair, BritiSh University. Dubai, United Arab Emirates

Assoc Prof. Dr. Pramod Kumar Gupta, Department of Civil Engineering, I IT Roorkee, India Prof Dr. Shahrokh Mirzahosseini, President, AVlcenna International College - Budapest (Hungary) & Member of the European Academy of Sciences and Arts

Program Committee (BBMPS-18) Conference Chair

Prof. Dr. Rahim Ahmadi, Islamic Azad University, Hamedan Branch, Iran; International Avicenna

College, Hungary, European Union

Prof. BuJent TOPCUOGLU, Akdeniz University, Vocational School of Technical Sciences, Antalya,

Turkey

Technical Committee

Dr. Md. Aminur Rahman, Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti

Putra Malaysia (UPM), Malaysia

Prof. Nahed Mohamed Mohamed Ismail, Theodor Bilharz Research Institute (TBRI), Egypt

Prof. Youcef NECIB, Universite Constantine1, Algeria

Assoc. Prof. Dr. Bidyut Saha, Department of Chemistry, Burdwan University, Burdwan, WB, India

Prof. Abid-UIRazzaq I Abu-Tair, British University, Dubai, United Arab Emirates

Prof. Seung-Hoon Han, Chonnam National University, Korea

Assoc. Prof. Mariam Gaidamashvili, Department of Biology, Faculty of Exact and Natural Sciences,

Iv. Javakhishvili Tbilisi State University, Georgia

Dr. Deena Elsori, Assistant Professor of Biology, College of Arts & Sciences, Abu Dhabi University,

UAE

Prof. Dr. Emhemmed Abdullah Alhibshi, Chemistry department, Faculty of Science, Aljabel Algharbi

University, Libya

Dr. Amirhossein Pirmoradi, Department of Agricultural Development, Razi University, Iran

Prof. Dr. Naime Arslan, Science and Art Faculty, Biology Department EskiAVehir Osmangazi

University, Turkey

Prof. Dr. Alina BUTU. National Institute of Research and Development for Biological Sciences,

NIRDBS, Bucharest, Romania

Ass! Prof. Yu Meng, Kean University, USA

Dr. Puthalapattu Reddy Prasad, Department of Chemistry, SVCollege of Sciences, Sri

Venkateswara University, Tirupati, India

Dr. Hemantkumar P Bulsara, In charge - Management section, Applied Mathematics and

Humanities Department, S V National Institute of Technology, Surat, India

.~

INDEX April 17-18. 2018 Kyoto (Japan)

11 th International Conference on Chemical. Agricultural. Biological and

Paper ID

C0418116

C0418123

C0418130

C0418131

C0418133

(0418135

C0418142

C0418143

C0418150

C0418151

C0418102

Environmental Sciences (CARES-20l8) Title of the Paper and Authors

Effects of Altitude on Physical Bean Quality of Robusta Coffee (Coffea

Canephora) Produced in Different Indonesian Regions

Onida Nor Marsilani, Wagiman and Anggoro Cahyo Sukartiko

Effect of Synthesis Method and Amount of Silver on Physicor:;hemical

Properties and Photocatalytic Activity ofAg Doped Ti02 Nanoparticles

Adilah Sirivallop, Siriluk Chiarakorn and Thanita Areerob

The Biomass Culture of Marine Phytoplankton Dunaliella Sp. in Arschat and Walne Medium by Compare Cel/s Concentration and Chlorophyl-a

Ito Purnomo and Arifin

Simulating CO2 Emissions from Tropical Forest Soils by Denitrification­Nitrification Decompasition (ONDC) Model

Amnat Chidthaisong and Phongthep Hanpattanakit

Study of Horseshoe Crab as Bycatch Around Bottom Gil/net in Mayangan

Waters, Subang, West Java

Fahresa N Supadminingsih, Mochammad Riyanto and Ronny I Wahju

Solid Waste Utilization of Tea Extract Industry as a Briquette and Super

capacitor based on Activated Carbon

Tiana Rafmiwati, Joshua Foliadi and Ayu Diah Padyanawati

Utilitation of Milk Package Waste as Source of Poly Aluminium Chloride

(PAC) Coagulant for Purification of Peat Water Rita Susanti, Muhdarina and Tengku Ariful Amri

Phytochemical Screening and Antioxidant Activity Stem Bark Extract of

Xylocarpus Granatum from Nothern Buton Arman Rusman, Sriwulan Purnamasari and Haeruddin

Mangrove Forest as a Balancing Ecosystem and Source of Raw Materials of Mangrove Syrup in Raas Island, Indonesia

Rizal Kartika Wardhana and Vegi AJfindo Nantaris

Recycling of Domestic Plastic Waste into Hippoplast as an Alternative

Media Plant

Vegi Alfindo Nantaris and Rizal Kartika Wardhana

Invistigation of Pol/ution Caused by Two Metals (Ni and Pb) in Coral

Ecosystems, Iran

Alireza Pourkhabbaz, Marzieh Nazeri, Farzad Mehrjo

Page No.

1

7

14

20

29

34

43

50

54

58

62

Paper ID

C0418103

C0418105

C0418106

C0418107

C0418110

C0418111

C0418112

C0418113

C0418117

C0418118

C0418119

Title of the Paper and Authors

Cultivation a Chiarella Sorokiniana AK-l With Swine Wastewater for Simultaneous Algal Biomass and Protein Production

Chun-Yen Chen, En-Wei Kuo and Jo-Shu Chang

Inhibitory Effects of Spore-Forming Microorganisms from Antarctica on the Growth of Various Bacterial Strains Sheau ling Yong, Clemente Michael Vui Ling Wong, and Marcelo A. Gonzalez

Employing Fermentation Strategies to Enhance Lipid Production EffiCiency with Thraustochytrium Sp. BM2 and Evaluation of Upgroding Applications

Chun-Yen Chen, Meng-Hsiu Lee and Jo-Shu Chang

The Use of Fish Gelatin and Alginate Blend as Gelling Agents for Pudding containing Virgin Coconut Oil Hui Yin Fan and Brenda Zhijia Ko

Isolation of Peripheral Blood Mononuclear Cell and Toll-like Receptor 4 Expression in White leghorn and Betong (KU line) Chicken

Anutian Suklek, Chaiwat Boonkaewwan, Autchara Kavan and Jatuporn Rattanasrfsomporn

Assessment ofAnti-inflammatory Activity ofSteviol in Human CD14+- Cells Jatupom Rattanasrisomporn, Waraporn Boonkaewwan, Autchara Kavan and Chaiwat Boonkaewwan

"Web of Love: Bonding Teeth per Enceinte" PER Model of Oral Health Promotion in the Pregnant Women WarapornBoonkaewwan, Kanvaphat Sutjai and Pennapa Boonkaewwan

The Study on Hematology and Immune Level to Newcastle Disease Virus in Betong (KU-Line) and Pradu Hong Dam Chicken Chaiwat Boonkaewwan, Anutian Suklek, Autchara Kavan and Jatuporn Rattanasrisomporn

Diagnostic Efficacy of Pleural Fluid Cytology in Feline Pleural EffUSion Natdaroon Chiteafea, Supreeva Srisampan, Ukadej Boonvaprakob, Wassamon Movadee, Pavu Srisuporn, Siwaporn Phooteng, Chaiwat Boonkaewwan, Autchara Kavan and Jatuporn Rattanasrisomporn

Modification of Corncob-based Cellulose with Succinic Anhydride Utilizing Microwave Assisted Organic Synthesis (MADS) Method Anif Fatmawatl, I Made Arcana and Deana Wahyuningrum

The Study of Antibody Titer of Newcastle Disease in White Leghorn Chicken Wltchavanun Nakkhao, Chaiwat Boonkaewwan, Autchara Kavan and Jatupom Rattanasrfsomporn

Page No.

63

64

65

66

67

68

69

70

71

72

73

ISBN 978-93-84422-85-1 11 th International Conference on Chemical, Agricultural, Biological and Environmental Sciences

(CABES-20IS) April 17-18,2018 Kyoto (Japan)

Assessment of Anti-inflammatory Activity of Steviol in Human CD14+ Cells

Jatupom Rattanasrisompom1, Warapom Boonkaewwan2

, Autchara Kayan3 and

Chaiwat Boonkaewwan3'

I 'Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine,

Kasetsart University, Bangkok, Thailand

::Department of Preventive and Social Medicine, Faculty of Medicine, Srinakharinwirot University,

Nakhonnayok, Thailand

3Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand

(*Corresponding author's e-mail: agrcwb@ku.ac.th)

Abstract: Steviol (SVO) is a major metabolite of stevioside which is a natural non-caloric Sll'eelener isolated from Sle\'ia rebaudiana Bertoni. The aim oflhe presenl srudy lI'as to illvestigate an inhibitory activity o/slel'iol on pro-in(lam/natOl:V cytokincs releaseFom lipopolysaccharide (LPS) -slimulated human CDJ4+ cells, CD I4+ cells. isohlled from human peripheral blood mononuclear cell (PBMC), \I'ere incubated lI'itll different concenlralions of Sleviol in the absence or presence oJ'LPS. The lel'el 0/ Tumor necrosis fifclor (T"'F)-a. IlIIerleukm (!L)-!fJ ((/'Id Inlerleukin rJL)-6 ~l'ere determined h.l· IISlIlg IllIman enzyme-linked immlll1050rbCIII assay (£US.-/) kils. Results demonstrated that sle,'iol at fhe cancelllnllioll of 1-100 JiM signijicaJ1lly inhibilerl (P<.O,rJ5)

the releuse o(TNF-Cl (966 n -l. 66.n, 906.3 .;. 36.7 and 6593· 52 3 IS 1190.3' 5.5 pg/ml) and IL-I/I f283·UI ,. 67.2, 2440.1J ;. 70.5 alld 2J81.3 + 143.3 vs 3226.7 + 106.6 p.r,11/11 ill a dose dependent l11alll1er II hen COII//Ir/red

la LPS-Iretlld CD14+ cells. ),Vhile lite inhibitioll o( !L-6 (p<orJ5) Has fillll1d ((/ I a JiA'! ((lid Iao II.~! (2171.7­44.3 CI/ld 1902.3 + 58.0 1'5 2580.3 + 105.2 pg/III/). 111 cOllclllsion.\{LTiol/,ossess (III (/Jlli-il1jlamllllllary Clui"in' ill

hUl1Iml CD! 4 t- celf.

Keyword.,· CDI4+, lL-IIJ. !L-6. celf, Sle\'iol, T,VF-ll

68

:

ISBN 978-93-84422-85-1 11th ternlU)cm:a1 C e nee on Chemical, Agricultural, Biological and Environmental Sciences

(CARES-20IS) April 17-18, 2018 Kyoto (Japan)

Diagnostic Efficacy of Pleural Fluid Cytology in Feline Pleural Effusion

Natdaroon Chiteafea', Supreeya Srisampan2, Ukadej Boonyaprakob3

, Wassamon Moyadee l,

Payu Srisupom' Siwapom Phooteng4, Chaiwat Boonkaewwan5

, Autchara Kayan5

and Jatupom Rattanasrisompom1

IDepartment of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

2Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

JDepartment of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

4Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand

50epartment of Animal Science, Faculty ofAgriculture, Kasetsart University, Bangkok, Thailand

(Corresponding author's e-mail: fvetjpn@ku.ac.th)

Abstract: Pleural ejfusion is lite abnormal conditioll of excess fluid acCutnulaiiol/ ill thoracic space and commonly occur in caiS. The objectil'f' of this study aimed to detennine the diagnosis a/pleural effusion in 30 cals at Kaselsart UniversilY Veterin{(JT Teaching Hospital. Thirty cats were used 10 SWe/.I' cytological evalulltion between December 2017 10 Janllary 2018. Tile clinical examination records of tilese cuts llcrc rel'iell'cd to

idenlifr the final diagnosis regardillg the etiology or Ihe pleural effUSion. C}tological el'alllillion ofpleural fillid has ;Iso been performed by Ihoraco( <.'111<'.1 is. Tlte r('slilts of tlte calise in pleural e[filsion of Ihe 30 C{lts rel'ealed thaI mediastina/lymphoma (II oIthe 3f) cMS, 36.67 percent). feline il!rectious peritOl/itis (9 ollhe 30 cots. 30,00 percelit), septic injectioll (8 or the 30 cats. 26.67 percent) and cardiol'UsCttlay dil('use (2 IIrlhe 30 cws. 6.67 perceltt). respectil'elj!. These .findillgs iltdiCOle Ihe mIlle (~r plpJ/}'oljJJlid C)-'lOjogy in /he dit;gJllJ.I'is v./ calS Huh

plcllral e/litsion.

Keywords: Cats. Cytological cl'ulll({/lOII. Flllid, Pleural.:jrusioll

71

ISBN 978-93-84422-85-1 11 th International Conference on Chemical, Agricultural, Biological and Environmental Sciences

(CABES-20IS) April 17-18,2018 Kyoto (Japan)

The Study on Hematology and Immune Level to Newcastle Disease Virus in Betong (KU-Line) and Pradu

Hang Dam Chicken

Chaiwat Boonkaewwanl', Anutian Suklek l

, Autchara Kayan l and Jatuporn Rattanasrisompom2

I Department of Animal Science, Facaulty of Agriculture, Kasetsart University, Bangkok, Thailand

lDepartment of Companion Animal Clinical Science, Faculty of Veterinary Medicine,

Kasetsart University, Bangkok, Thailand (*Corresponding author's e-mail: agrcwb@ku.ac.th)

Abstract: BelOng and Pradu Hang Dam Chicken are Thai native chicken which are very popular species. The objeclil'e oJthe present s/lldy aimed to determine the hematology and immune level to Newcastle disease virus in Betong (KU-/ine) and Pradu Hang Dam chicken at Vajokkasikit poulll}' research Jarm, Kasetsart University, Bangkok. Thailand. 16 \I'eeks old, 24 chicken (12 males and 12 Jemales) per breeds were used in this sll/dy. Blood and serum were collectedJor complete blood cOllnt and haelllogglutination inhibition teST. Tire cumplete hlood COlinT of BelOng (KU-/ine) chicken are as fiJ 110 w TOlal EryThrocyte count 2.2±O.8 cellimm3. Hemoglohin 9. 7±O.8 g/dl and Hematocrit 28.8±2.4 %. The difjerenlial leukocyte count of Heterophil. Eosinophil, Basophil, Lymphocyte alld A,fonocyte are 70.3±5.3 %. 1.3±1.6 %. O.O±O.O %,26. 7±3.9 % and 2.6±1.2 %. respedil'eh. The complete blood counl of Thai Native chicken are as /01/011': TOIOI Erythrocyte COIIIII 2.2:di.2 cell/mm3, Hemoglohill 8.6±O.8glrll and Hematocrit 26.0±2.8 %. The difjerel1lialleukocyte coulll of Heterophil. Eosilluphil. Basophil, Lymphocyte and "v/ol1ocyte are 60.4± .1 %. O.8±1.9 %, O.7±O.9 %, 34.9±8.9 % alld 3.3±1.2 %. respec/i\'(:(IJ. Results demons/rated the di(fi'rcnCl' ill Eruhrocyte CO/IIII, Heamoglobill. Hematocrit, Ifel<'l'0l'ilif. Eosinophil. Basophil. Lymphocyte and :\lollollcl'tc Ilheil compare /0 references I'aille. 17,(, pmsihle ,·'.plilllatioll should hI.! /he dij(C?rellces ill breed. n/wwgemenl. <!//I'imllillent lI//rljced. 1/1 additioll. immulle !tTl-f /0 Vell't'lls/Ie disease l'irm demons/rated that Pradll Hang Dum (fllt! BeTIIII,!!. rI\C'-lille) chicken are 3.2±O.3 ant! 2.9 :tV.3. respectil"c!l·. Pradll liang Dan/ chicken seem 10 1/11.. ' (f higher illin/line 1('I'elthall Belong rKI.·-IiIlf:) chickel!.

Keywords: Hen/awlogy. IlI1l11une lel'el, BelOllg Chit-kell. Pl'lldu Hall,!!. Dam chicken.

70

ISBN 978-93-84422-85-1 lIth International Conference on Chemical, Agricultural, Biological and Environmental Sciences

(CABES-2018) April 17-18, 2018 Kyoto (Japan)

Isolation of Peripheral Blood Mononuclear Cell and Toll-like Receptor 4 Expression in White leghorn

and Betong (KU line) Chicken

Anutian Suklek I, Chaiwat Boonkaewwan I', Autchara KayanJ and Jatupom Rattanasrisompom2

'Department of Animal Science, Facaulty of Agriculture, Kasetsart University,

Bangkok, Thailand

"Department of Companion Animal Clinical Science, Facau)ty of Veterinary Medicine,

Kasetsart University, Bangkok, Thailand (*Corresponding author's e-mail: agrcwb@ku.ac.th)

Abstract: Peripheral blood mononuclear cells (PBA1Cs) are a mixed population ofsingle nuclear white blood cells. The aim of Ihe presenl study is 10 examine Ihe cell viability of PBMCs isolated from While Lef{horn and BelOng (KU line) chicken by using Ihe Irypan blue dye exclusion lest. In addition, Toll-like receptor 4 expression in PBMC~ was also delermined by using PCR technique. Resulis demonstrated that cell lliabilily ofPBMCsji-om BelOnf{ (KU line) was grealer Ihan White Leghorn chicken (P«J05). PBMCs isolaledji-olJl whole blood that kept {f/ 4 °C for 0, 24 and 48 h of Belong (KU line) chicken has no difference in cell viability. kforevl'er. TLR4 \I'([S

<'xpressed in PB,'vICs from both breeds of chicken. Iii conclusion, PBMCs from While Leghorn ([nd Belong (KU line) chiLkel1 can be use as source for the assessment a/immune response to pathogens.

Keywords: PB,\1C~, celll'iabiIily. lOll-like receplOr 4. chicken.

67

11 th International Conference on Ch mj

The Study of Antibody Titer ofNe\:vcast' White Leghorn Chicken

Wjtchayanun Nakkhao', Chaiwat Boonkaewwan'·, A.ULClJdlCl r<:UJU": __.-'

SatupanI Raccaaa.>--rIso/npDJ77:?

IUe'\l'O.nmentC\t Animal Sc.ience, Facaulty of Agriculture, Kaset<;art University, Bangkok, Thailand

~Department of Compan\ol1 J\n\ma\ C\iuica\ ~c\.euce, r ac'O.'u\\') \)\ \[ete.nua!'i Medicine,

Kaselsart University, Bangkok, Thailand

(*Corresponding author's e-mail: agrcwb@ku.ac.th)

Abstract: White Leghorn chickens are known as efficient egg layers. They produce fi,}lile eggJ and wilJ lay more than many breeds while consuming less feed. Raising white leghorns requires quality housing. feed and vaccination program. The objectil'e of the present study aimed to determine Newcastle disease (ND) Antibody titer and the stability of the antibody titer after vaccination in 12 v,eeks old White Leghorn Chicken at Vajokkasikit poultry research fi:mn. Kasetsart University. 70 White Leghorn chickens (male and female) were separated ill 3 groups. Blood sampling were collected from "'hite leghorn 10 chicks per group. Haemaggllllination Inhibition (IfI) antibody test was used 10 determine the antibody titer of ND in 3 groups of White Leghom Chicken Results sholto that the antibody titer uf the .first group. before (week 0) and post­vaccination (It'eek 1-4), It'ere 1.2. 4.2. 2.6. 2.4 and 1.8 respeclive~v. TIlt' al1lihOf(1: titer o/the second group, before (H'eek 0) alld post-mccilliltioll (11'cek 1-4). were 1.5, 3.8, 2.5, 2.9 and 2.4 respectively. The antibody titer of tile third group, before (lI'ed 0) lind post-mccillation (week 1-4), ,..ere 1.7. 3.2. 3.6. 2.8 and 2.2 re~pectij!e~y. These results rel'ealed that the highest antihody liter to Nel1"C£tSlle Disease IIIIS ./ii/llld lit the first lVeek of post­I'accillation (P< 0.(5). Tlte antihody tiler 'l"t're then decreased in lI'eel, 2. 3 alld 4 {~fier "accinalion. ,"'torenner, the al1libody titer al IhejiJllrtfl 11'eel< orpost-Vflccillatioll was close to tIle le,'('/ {~/antihndy titer be{oreyaccinlltioll. In conclusioll. IIntihody Iller 10 YD ' ..as highest in the .first Ileek or post-l'IIccinlllion and the al1libody titer remained higher/hr.f lI·ech.

Keywords: neIlLflst/" diseOSll, \I'hile leghurJI chicken, wltihod}' titer

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