Kickoff meeting of MarPlanKickoff meeting of MarPlanSeptember 23rd September 23rd

NapoliNapoli

Observatoire de VillefrancheObservatoire de VillefrancheLaboratoire d’Océanographie de Laboratoire d’Océanographie de

VillefrancheVillefranche

Maria Luiza PedrottiMaria Luiza Pedrotti Rodolphe LeméeRodolphe Lemée

Alina TuninAlina TuninJohn DolanJohn Dolan

Illustrations from website: www.obs-vlfr.fr

Observatoire Observatoire Océanologique Océanologique

de Villefranche-sur-Merde Villefranche-sur-MerFrench coast of NW Mediterranean Sea

Zoological Station

Bay of Villefranche-sur-Mer

Observatoire

- 130 permanent researchers or technicians- Surface : 7 000 m2

- One restaurant (for lunch) and accommodation

Bay of Villefranche-sur-MerDaily plankton samples

Sagitta

Vellele

Observatoire Océanologique Observatoire Océanologique de Villefranche-sur-Merde Villefranche-sur-Mer

University of Paris VI (with Banyuls and University of Paris VI (with Banyuls and Roscoff) and CNRS: National Center of Roscoff) and CNRS: National Center of Scientific ResearchScientific Research

Center of Research, Monitoring and TeachingCenter of Research, Monitoring and Teaching

Teaching: Masters with marine ecology Teaching: Masters with marine ecology (pelagic and benthic systems), plankton (pelagic and benthic systems), plankton diversity, biogeochemistry, numerical diversity, biogeochemistry, numerical ecology, geology.ecology, geology.

MonitoringMonitoring

DYFAMED - JGOFS station28 miles offshore

Pt B SOMLIT station

GeosciencesGeosciences Azur AzurResearch topics:Research topics:

- History, - History, mechanical mechanical properties and properties and deformation of deformation of the lithosphere, the lithosphere, - Natural hazard - Natural hazard assessmentassessment

ResearchResearchMultidisciplinary research center with 3 Multidisciplinary research center with 3 laboratories:laboratories:

Developmental BiologyDevelopmental Biology

Reaserch topics: Reaserch topics: - Cell cycle control- Cell cycle control- Embryonic development of marine - Embryonic development of marine invertebrateinvertebrate

Expertise:Expertise:- Light microscope imaging of calcium, - Light microscope imaging of calcium, cytoskeleton and organelles in live cellscytoskeleton and organelles in live cells- Subcellular visualisation and - Subcellular visualisation and localisation techniques localisation techniques (immunolocalisation at light and (immunolocalisation at light and electron microscopy, fast-freeze/deep electron microscopy, fast-freeze/deep etch)etch)- Biochemistry- Biochemistry- Molecular Biology (cloning, expression - Molecular Biology (cloning, expression studies, studies, in situin situ hybridisation) hybridisation)

Laboratoire d’Océanographie de Laboratoire d’Océanographie de Villefranche (LOV)Villefranche (LOV)

31 researchers, 21 engineers/technicians, 31 researchers, 21 engineers/technicians, 10 post-Doctorals positions and 18 Ph.D10 post-Doctorals positions and 18 Ph.D

5 research groups specialized in pelagic 5 research groups specialized in pelagic systemssystems Marine optic and remote sensingMarine optic and remote sensing GeochemistryGeochemistry Dynamic of planktonDynamic of plankton Biological and physiological answers to stressBiological and physiological answers to stress Marine microbial ecology (involved in Marine microbial ecology (involved in

marPLAN)marPLAN)

Marine optics and remote sensingMarine optics and remote sensing

BOUSSOLE: New platform dedicated to optical Observations in the surface layer of the ocean

It is an interdisciplinary groupIt is an interdisciplinary groupstudying the interaction of solarstudying the interaction of solarof solar energy with the ocean.of solar energy with the ocean.

GeochemistryGeochemistry

Effect of nutrients and metals on pelagic systemsEffect of nutrients and metals on pelagic systemsEvolution of primary production and pigment diversityEvolution of primary production and pigment diversity

in NW Mediterranean Sea (monitoring at DYFAMED site)in NW Mediterranean Sea (monitoring at DYFAMED site)

Dynamic of planktonDynamic of plankton Plankton diversity and structuration of pelagic food web in relation to Plankton diversity and structuration of pelagic food web in relation to

trophic and environment. trophic and environment. Role of key species in energy transferRole of key species in energy transfer Phytoplankton ecophysiology and modelingPhytoplankton ecophysiology and modeling

Biological and physiological answersBiological and physiological answers to environmental stressto environmental stress

Spatio-temporal variability and Spatio-temporal variability and adaptativeadaptativestrategies of microzooplankton and strategies of microzooplankton and nectonnectonQuantification of zooplankton impact Quantification of zooplankton impact on biogeochemical cycleson biogeochemical cycles

Marine microbial ecologyMarine microbial ecology

3 majors topics of the group:3 majors topics of the group: Factors regulating activities and structure of Factors regulating activities and structure of microbial populations (external forcing, top-microbial populations (external forcing, top-down, bottom-up controls)down, bottom-up controls) Functional and taxonomic diversity in Functional and taxonomic diversity in microbialmicrobial

populations: variation in time-space scalepopulations: variation in time-space scale Roles of microbes in biogeochemicals Roles of microbes in biogeochemicals processesprocesses

(dynamics of DOM, TEP and CO(dynamics of DOM, TEP and CO22))

7 researchers, 2 engineers/technicians, 3 post-Doctoral 7 researchers, 2 engineers/technicians, 3 post-Doctoral researchers, 4 Ph.D students and 6 or 7 master researchers, 4 Ph.D students and 6 or 7 master

students/yearsstudents/years

Marine microbial ecologyMarine microbial ecologyPermanent researchers and expertises Permanent researchers and expertises

Involved in MARPLAN-John Dolan, group leader:

Diversity and ecology of ciliates-Rodolphe Lemée, assistant professor:

Diversity and ecology of microphytoplankton-Maria Luiza Pedrotti, research scientist: Bacterioplankton

diversity and role of TEP in the biogeochemical cycles

-Alina Tunin (Ph.D -University of Paris VI, Ibanez and Lemée): Short, middle and long-term changes in Ceratium diversity

Others-Jean-Pierre Gattuso, senior research scientist:

Biogeochemical processes, bacterioplankton respiration-Emilio Maranon, research scientist:

Ecophysiology of phytoplankton-Fereidoun Rassoulzadegan, senior research scientist:

Microbial ecology, effect of nutrients on marines microbes -Markus Weinbauer, research scientist:

Diversity of marine bacteria and viruses

John

Maria Luiza

Rodolphe

Alina

Marine microbial ecologyMarine microbial ecologyEquipment and techniques:

Epifluoresence microscopes with image analysis

Coulter counterFlow cytometer (FacsCalibur)

TOC analyzer (Shimadzu)Tangential flow filtration system (Millipore)

Titrators for O2 and total alkalinity determinations

High precision pH metersIncubators, Spectrometer

System for pCO2 control of algal culturesRespirometers with Peltier-regulated

temperatureFISH, CARD-FISH

Pulse gel electrophoresis, PCR, DGGE

Long-term changes in Ceratium (dinoflagellate) diversity in Ligurian and Tyrrhenian seas

Alina Tunin and Rodolphe LeméeAlina Tunin and Rodolphe LeméeNeeds:Needs: Old and recent net samples of microphytoplankton Old and recent net samples of microphytoplankton Literatures from cruises, reports and old papers Literatures from cruises, reports and old papers on distribution and annual cycle of on distribution and annual cycle of CeratiumCeratium

John DolanJohn DolanNeeds:Needs:Samples from diverse Samples from diverse sites, sites, expertise in expertise in morphometricsmorphometrics

Relationship between morphological and taxonomic diversity in tintinnid cilates

Specific needs from the collaboration within MarPlanSpecific needs from the collaboration within MarPlan

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Phylogenetic relations at specific and subspecific levels in genus Ceratium

Rodolphe LeméeRodolphe Lemée Needs:Needs:

« Single cell PCR technique » :Research collaboration « Single cell PCR technique » :Research collaboration during 2005 and 2006 with phytoplankton group of Roscoffduring 2005 and 2006 with phytoplankton group of Roscoff

Several Ceratium tripos

Illustration from Sournia

Same specie ?

Bacterial diversity and dynamic of particles (TEP transparent exopolimeric particles)

Maria Luiza PedrottiMaria Luiza Pedrotti NeedsNeeds: : Exchanging samples and calibration protocols for Exchanging samples and calibration protocols for

FISH and CARD-FISHFISH and CARD-FISH

What bacterial group colonize particles ?

Bacteria TEP Superposition

HRP