Lab Exercise #7

Epithelial Tissues

Let’s review….

Simple cuboidal epithelium

lining a tubule (longitudinal cut). Some of the cell boundaries between "blocks" or "cubes" here are quite distinct.

Simple Cuboidal

Covers surface of ovary, lines kidney tubules and small ducts of glands (thyroid and pancreas)

Tubule - cross sectionsimple cuboidal

Another example - kidney

Type?

Simple squamous epithelium

High power view of endothelial cells lining a small blood vessel cut in cross-section. (You see just the nuclei - the cytoplasm between them is extremely flat.) Endothelium = the simple squamous epithelium lining blood vessels.

Simple squamous epithelium

Kidney

Superficial view (squamous)

Type?

Simple columnar

Another example – intestine, with goblet

Intestinal lining

Yet another

Type?

Pseudostratified ciliated columnar epithelium

Trachea. Nuclei are at different levels. All cells touch the basement membrane, but only the taller cells reach the lumen. The cilia are longer and less regular than the microvilli of a striated border.

Pseudostratified Columnar Epithelium Look like multiple layers Trachea

Figure 4–5b

Another example

Cilia – surface view

Type?

Bladder Low power

Transitional Epithelium Tolerates stretching Appearance changes (stratified to simple) Urinary bladder

Figure 4–4c

Type?

Figure 4–3b

Stratified Squamous Epithelium

Example

Example

Type?

Type?

Stratified Cuboidal Epithelium

Sweat gland ducts

Figure 4–4b

Type?

Stratified Columnar Epithelium

Rare Salivary gland duct

Figure 4–5c

Note

It is the shape of the most superficial layer that determines what type of stratified epithelium it is.

Today’s procedure

Set up microscope Get a slide Follow instructions in Lab Manual Hints

Start on low power and locate epithelium (this is the bulk of the work)

Go to 10x, then 40xDraw tissueReturn slide to box you got it from.

Today’s lab

Examine and draw at least one of each type of epithelial tissue

Activity 1:Simple squamous – two options: kidney

glomeruli, mesotheliumSimple cuboidal – kidney tubulesSimple columnar – large intestine (frog,

human)

Today’s lab

Examine and draw Activity 2:

Psuedostratified ciliated columnar – trachea Transitional – ureter

Today’s lab

Examine and draw Activity 3:

Stratified squamous (skin, oral smear, frog superficial view)

Stratified columnar (salivary gland)No stratified cuboidal (don’t need to do)

Lab Write up

Turn in: 7 drawings on page I provide Parts A, B, C, only Due next week on TUes