Nucleic Acid and ProteinIsolation Reagent

TRI Reagent is an improved version of asingle-step method of total RNA isolationfrom samples ofhuman, animal, plant, yeast,bacterial, and viral origin. It offers simulta-neous isolation ofRNA, DNA, and proteinsfrom a single biological sample. RNA is iso-lated in less than 1 hour; DNA and proteinsin 3 hours. Molecular Research Center.Circle 138.

Gel Plate TreatmentGelRepel coating system eases removal ofpolyacrylamide gels from glass plates.GelRepel is a patented system of catalyst-activated isomeric polymers solubilized inalcohol. The system forms a hydrophobic

monomolecular coating that is chemicallyand physically bonded to the glass to makethe surface virtually nonwettable. The usersimply wipes GelRepel onto the plates, andthe coating lasts for hundreds of runs.GelRepel is nontoxic and biodegradable.Aardvark Science. Circle 139.

m

Monoclonal AntibodiesThe AntiFLAG BioM2 monoclonal anti-body (mAb) is a biotinylated version of theAnti-FLAG M2 mAb. It can be detected bystreptavidin- or avidin-conjugated enzymes.Eastman Kodak. Circle 140.

CDLa mAb performs in fixed, paraffin-em-bedded tissue sections. In normal cells, itreacts with cortical thymocytes, Langerhans'

cells, and interdigitating reticulum cells. Italso reacts with thymonas, Langerhans'histiocytosis cells, some T cell lymphomas,and leukemias. Immunotech. Circle 141.

Two new mouse mAbs detect estrogen re-ceptor on cryostat sections and formalin-fixed, paraffin-embedded tissue sections, en-abling work to be performed on archive ma-terial. Serotec. Circle 142.

a

PCR Detection SystemThe TaqMan LS-50B PCR Detection Sys-tem is designed to make polymerase chainreaction (PCR) a routine assay for quantita-

tive sequence detection. Unlike gel-basedapproaches, the system makes use of a 5' nu-clease assay incorporated into the TaqMan

Wit FACE, as soon as bands appearyou know it's carbohydrate.

FACE (Fluorophore Assisted * CCarbohydrate Electrophoresis) higives youfast, reliable, and elevisual proofthat carbohydrate amis there.

* FMUcNA.~Um\ CO(

d4ISNco~~m\ to]_um-UcNA4IcNA exl

* FAcor

aI-UcfIA.-Uu. to

* FACqudo(

-- cMuNAdM*TOseiU.

, 'U3 12us

01995 GLYKO, INC. FACE ISA REGISTERED TRADEMARK OF GLY(0, INC.

rKO'S FACE TECHNOLOGY uses a familiar andghly reliable technique-polyacrylamide gelnctrophoresis-to separate complex carbohydrates,Id gives you results you can actually see.

CE CHEMISTRY KWS come in easy-to-follow, color-ded packaging and include everything you needperform carbohydrate profiling or compositionpenments.CE RECOMBINANT LYCIDAEs (the mostrnplete line available anywhere) make it possiblesequence oligosaccharides right in your own labto matter what separation technique you employ.

CE MAR AND ANALYC SOFWARE provide atick, reliable way to analyze, quantify, andcument N-linked and 0-linked oligosaccharideofiling gel results.

LEA MR about Glyko products, customrvices, and international distributors, call toll freeS. only: 1 800 33 GLYKO (334 5956), phone:415 382 6653, fax: 1 415 382 7889, or write toat 81 Digital Drive,wvato,California94949.

Circle No. 27 on Readers' Service Card

PRODUCTS &MATERIALS

1 MW

m

on May 18, 2021

http://science.sciencem

ag.org/D

ownloaded from

PCR Reagent Kit. The kit includes PCRprimers and a fluorogenic probe that emitsfluorescence only if the target sequence isamplified. After the samples and reagents

are pipetted into reaction tubes, the tubes are

sealed and transferred to the thermal cyclerfor amplification. A sequence-specific signalis generated in solution during PCR. AfterPCR, the products are transferred to 96-wellplates for detection by a luminescence spec-

trometer. The simplicity of the process sig-nificantly reduces the chance of laboratorycontamination by DNA and reduces thequantitative detection of sequences to a fast,reliable format. Perkin-Elmer. Circle 143.

a

Double-Stranded Plasmid DNAA rapid plasmid preparation kit is designedto purify up to 200 pg of double-strandedplasmid DNA. The Hybaid Recovery Plas-mid Midi Prep Kit generates DNA suitablefor all uses, including automated fluorescentsequencing and transfection, without theneed for hazardous reagents such as phenoland chloroform. Once the culture has beenspun down, cells are suspended in 500 p1 ofwater and transferred to the supplied micro-centrifuge tube for lysis. DNA extractionfrom the lysed cells takes place using a silicamatrix binding buffer and a spin filter.Hybaid. Circle 144.

Fusion Protein Purification KitThe Fusion Protein Purification Kit allowspurification of the fusion protein both fromits fusion partner and the cleaving protease.Previous kits produced samples contaminatedwith either the protease or tne fusion part-ner. Applications for the new kit include thepurification of cloned proteins, protein ex-

pression studies, protein purification andcharacterization, sequence analysis of proteinsand peptides, and synthesis of peptide hor-mones. Boehringer Mannheim. Circle 145.

a

Immune System Response AssayThe FastImmune assay system is for assess-

ing immune function of lymphocyte popula-tions in whole blood. The assay directly as-

sesses individual T-lymphocyte subset re-

sponses to a wide variety ofphysiological andnonphysiological stimuli in under 5 hours.Traditional bulk population assays can takeup to 10 days and provide limited informa-tion. The system consists of two- and three-

color reagents, isotype and activation con-

trols, and a rapid, no-wash procedure.Becton Dickinson Immunocytometry Sys-tems. Circle 146.

Nitric Oxide AnalyzerThe 270B Nitric Oxide (NO) Analyzer isbased on the chemiluminescent reaction ofNO with ozone. The rapid, sensitive methodmeasures trace levels of NO in both liquid

and gas samples. In liquid samples such as

tissue perfusates, cell cultures, and plasma,NO, nitrite, and nitrate can be measuredthrough a unique purge system. The 270Bcan also be used for measuringNO in expiredbreath because the gas phase response time is250 ms. The detection limit is 1 pmol forliquids and 5 ppb for gases with a linear re-

sponse over four orders of magnitude. SieversInstruments. Circle 147.

LiteratureOmni International: Homogenizing, Emulsify-ing, Dispersing, Mixing outlines a completeline of mechanical shear, bench-scale ho-mogenizers, and accessories. Omni Interna-tional. Circle 148.

Mass Standards Handbook is a weighing refer-ence publication that clarifies the applica-tion, classification, and physical characteris-tics of precision weights and mass standards.Troemner. Circle 149.

cDNA Synthesis and Cloning is a manual withprotocols for every aspect of complementaryDNA (cDNA) library construction. It in-cludes an overview, procedures, and trouble-shooting guide for RNA isolation, cDNAsynthesis, and the cloning ofcDNA libraries.Pharmacia Biotech. Circle 150.

- -~~~d

source for Innovativekits for all your nucleicacid labeling needs

NEW! Kinase Maxzc5' End Labeling Kit produces 1.5-3times the yield than kits utilizingstandard kinase buffers.

DECAprime. 11For high specific activity DNA label-ing with the random primingmethod. Fast five minute reactionmaximizes probe yield and specificactivity.

NEW! Prime-A-ProbeTMFor strand specific DNA probe syn-thesis. The kit utilizes specific primersand your linearized plasmid DNA toproduce high specific activity probesfor Si, Southern or Northern assays.

Contact us todayforinformation on ourfull range of

nucleic acid labeling kitsforyour specific applications.

(512) 445-6979 (512) 445-7139 FAX

(800) 888-8804

Am, nAe.

Circle No. 48 on Readers' Service Card

SCIENCE * VOL. 268 * 16 JUNE 1995

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines inacademic, industrial, and government organizations are featured in this space. Emphasis is given to purpose, chiefcharacteristics, and availability of products and materials. Endorsement by Scienceor AAAS is not implied. Additionalinformation may be obtained from the manufacturers or suppliers named by circling the appropriate number on theReaders' Service Card and placing it in a mailbox. Postage is free.

----- - ---- Ill ll li ii li ill

1643

on May 18, 2021

http://science.sciencem

ag.org/D

ownloaded from

PRODUCTS & MATERIALS

DOI: 10.1126/science.268.5217.1642 (5217), 1642-1643.268Science 

ARTICLE TOOLS http://science.sciencemag.org/content/268/5217/1642.citation

PERMISSIONS http://www.sciencemag.org/help/reprints-and-permissions

Terms of ServiceUse of this article is subject to the

is a registered trademark of AAAS.ScienceScience, 1200 New York Avenue NW, Washington, DC 20005. The title (print ISSN 0036-8075; online ISSN 1095-9203) is published by the American Association for the Advancement ofScience

1995 by the American Association for the Advancement of Science

on May 18, 2021

http://science.sciencem

ag.org/D

ownloaded from