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Lecture 21 HPLC-2 Instrumentation

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Page 1: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Lecture 21

HPLC-2Instrumentation

Page 2: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

ColumnInjector Detector

Chromatograph

Data Processing

Page 3: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 4: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 5: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 6: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Percentage of solvents having the same eluent strength

Page 7: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

B - acetonitrile CH3CN

Page 8: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

constant

constant constant 162

2

2

2

W

t

W

t

W

tN

r

rr

Width W is proportional to retention time tr

‘general chromatography problem’

Page 9: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 10: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Solution of GNP: gradient

Page 11: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 12: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Pump

Page 13: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

4.5 mm2 HPLC columns of different length

Page 14: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Injector

Page 15: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Detectors

Page 16: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

UV cell

Page 17: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 18: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Derivatization in HPLC

Page 19: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing
Page 20: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

Evaporative Light- Scattering Detector

Page 21: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

HOOH

HO1

HOOH2

HOOH3

CH3OH

CH3CH2OH

OH

4

5

6

OH7

OHHO

O OH

13 14

Electrochemical detection of alcohols in water

8 2-butanol 9 2-Methyl-1-propanol10 1-butanol 11 3-methyl-1-butanol12 1-pentanol

Chemistry: Oxidation of alcohols @Au electrode

Page 22: Lecture 21 HPLC-2 Instrumentation. Column Injector Detector Chromatograph Data Processing

MS Detector


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