Lipids

Structure and function

Lipids are a mixed group of hydrophobic compounds composed of the elements carbon, hydrogen and oxygen.

They contain fats and oils (fats are solid at room temperature, whereas oils are liquid)

triglycerides Glycerol is a

small, 3-carbon molecule with three hydroxyl groups.

triglycerides Fatty acids are long

molecules with a polar, hydrophilic end and a non-polar, hydrophobic "tail". The hydrocarbon chain can be from 14 to 22 CH2 units long. The hydrocarbon chain is sometimes called an R group, so the formula of a fatty acid can be written as R-COOH.

Saturated fatty acids If there are no

C=C double bonds in the hydrocarbon chain, then it is a saturated fatty acid (i.e. saturated with hydrogen). These fatty acids form straight chains, and have a high melting point.

Triglycerides containing saturated fatty acids have a high melting point and tend to be found in warm-blooded animals. At room temperature they are solids (fats), e.g. butter, lard.

Unsaturated fatty acids If there are C=C double

bonds in the hydrocarbon chain, then it is an unsaturated fatty acid (i.e. unsaturated with hydrogen). These fatty acids form bent chains, and have a low melting point. Fatty acids with more than one double bond are called poly-unsaturated fatty acids (PUFAs).

Triglycerides containing unsaturated fatty acids have a low melting point and tend to be found in cold-blooded animals and plants. At room temperature they are liquids (oils), e.g. fish oil, vegetable oils.

Triglycerides Triglycerides are insoluble in water. They are used for storage, insulation and protection in fatty tissue (or

adipose tissue) found under the skin (sub-cutaneous) or surrounding organs.

They yield more energy per unit mass than other compounds so are good for energy storage.

Carbohydrates can be mobilised more quickly, and glycogen is stored in muscles and liver for immediate energy requirements.

Lipid testsThere are two types of chemical test commonly carried out to test for lipids.

1. Emulsion testadd 2cm3 of lipid to a test tube containing 2cm3 of absolute ethanol. Dissolve the lipid by shaking vigorously. Add an equal volume of cold water.

This will give a cloudy white suspension.

2. Sudan IIISudan III is a red dye. Add 2cm3 of lipid to 2cm3 of water in a test tube. Add a few drops of Sudan III and shake.

A red oil layer separates on the surface of the water

Milk contains saturated triglycerides. We can purchase different types of milk:

Full fat Semi – skimmed Skimmed

Each type of milk has a different quantity of fat. The fat is removed to provide a healthier option for consumers.

Phospholipids

Phospholipids have a similar structure to triglycerides, but with a phosphate group in place of one fatty acid chain. There may also be other groups attached to the phosphate.

Phospholipids have a polar hydrophilic "head" (the negatively-charged phosphate group) and two non-polar hydrophobic "tails" (the fatty acid chains).

This mixture of properties is fundamental to biology, for phospholipids are the main components of cell membranes.

Phospholipids When mixed with

water, phospholipids form droplet spheres with the hydrophilic heads facing the water and the hydrophobic tails facing each other. This is called a micelle.

Phospholipids Alternatively, they may

form a double-layered phospholipid bilayer. This traps a compartment of water in the middle separated from the external water by the hydrophobic sphere. This naturally-occurring structure is called a liposome, and is similar to a membrane surrounding a cell.

Other classes of lipids Waxes Waxes are formed from fatty acids and long-chain

alcohols. They are commonly found wherever waterproofing is needed, such as in leaf cuticles, insect exoskeletons, birds' feathers and mammals' fur.

Steroids Steroids are small hydrophobic molecules found mainly

in animals. They include: cholesterol, which is found in animals cell membranes

to increase stiffness bile salts, which help to emulsify dietary fats steroid hormones such as testosterone, oestrogen, and

progesterone. vitamin D, which aids Ca2+ uptake by bones.

questions1. Draw a diagram to show the formation of a

triglyceride from one molecule of glycerol and three molecules of fatty acid.

2. A lipid is insoluble in water. How does the addition of a phosphate group affect this property?

3. What does hydrophilic mean?4. What does hydrophobic mean?5. Why are phospholipids important in the

body?