Live Primary Neuronal CellsLive Primary Neuronal CellsNeuroPure™

Live Primary Neuronal CellsNeuroPure ™

Live Primary Neuronal Cells

GenlantisGenlantisa division of Gene Therapy Systems, Inc.

• Freshly isolated healthy cells – not frozen• Ready to use – primary culture up and running within 1 hour• Exceptional purity – 99.9% glial cell free• Easily transfected with NeuroFECT™ reagent• Guaranteed quality and consistency

Available from ams biotechnology (europe) ltd

info@amsbio.com - www.amsbio.com

Save Time with Fresh, Ready-to-use Primary Neuronal CellsCultured primary neuron cells are important enabling tools in manyaspects of neuroscience research. However, consistently obtaining pureand healthy cultures is not trivial, requiring both a significant timeinvestment and surgical expertise. To help streamline your research,Genlantis offers ready-to-use primary rat neurons, astrocytes, and neuronalprogenitor cells, which are prepared fresh each week to meet your needs.

NeuroPure Live Primary E18 Rat NeuronsThe NeuroPure™ neuronal tissues are micro-dissected from distinct brainregions of Sprague/Dawley or Fischer 344 rats. They are shipped live innutrient rich medium that keeps the cells viable for up to 2 weeks.Following a rapid protocol to dissociate the cells, your primary culturecan be ready in less than 1 hour upon receiving the shipment.The following popularly used cell types are available:

• P8 Cerebellum • E18 Cortex

• E18 Hippocampus • E18 Hypothalamus

• E18 Midbrain • E18 Spinal Cord

• E18 StriatumUnlike other commercially available neurons, NeuroPure cells are 99.9%glial cell free (see below). The uniform cell population makes NeuroPurecells ideal for a host of applications including gene expression analysis,neuro-development,neurotoxicity, receptor signaling,and electrophysiology.

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Figure. Comparison of NeuroPure Cells with Frozen Competitor CellsTo evaluate the purity of cells, rat hippocampal cells were fixed and double immunostained with the greenneuronal cell marker FITC-anti-MAP2 (A) and the red glial cells marker Rhodamine-anti-GFAP (B).

NeuroPure Primary E18 Rat Hippocampal Cells

Competitors Frozen Rat Hippocampal Cells

Panel A. NeuroPure cells are ~99.9% pure and show long dendrites.All cells were stained by the green neuronal cell marker anti-MAP2. Panel B. Glial cells are not present as shown by the lack of red staining by anti-GFAP marker.Panel C. Morphology and viability of NeuroPure cells are > 70%.

Panel A.The purity of competitors cells was ~ 50%. Rat hippocampal cells were stained by the green neuronal cell marker anti-MAP2.Panel B. Competitor’s hippocampal cells contain a large amount of glial cells as shown by the red staining from the anti-GFAP marker.Panel C. Morphology and viability of competitor’s cells is estimated at 14%.

NeuroPure™ Live Primary Neuronal CellsNeuroPure Rat Hippocampal Neurons

NeuroPure Rat Cortical Neurons

NeuroPure Live Primary Rat AstrocytesAstrocytes were once considered only as a supporting actor in brainfunction. Today, with the discovery of signaling between neurons andastrocytes and among astrocytes themselves, astrocytes are starting totake over some of the spotlight that was focused on neurons. Togetherwith the popular NeuroPure primary rat neurons, NeuroPure astrocytesprovide an ideal model system for researchers to gain insights into thefunction of these cells.

NeuroPure primary rat astrocytes are freshly prepared from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley orFischer 344 rat brain. They contain greater than 95% astrocytes and areshipped live in nutrient rich media that keep the cells viable for 2 weeks. The following astrocytes are currently available from Genlantis:

• Astrocytes from E18 Rat Hippocampus

• Astrocytes from E18 Rat Cortex

NeuroStem™ Live Rat Neuronal Progenitor CellsNeuroStem™ Neuronal Progenitor Cells are pluripotent cells that arecapable of differentiating into neurons, astroglia, or oligodendrocytes.They can multiply for many generations under appropriate cultureconditions. When cultured in media with low adhesion substrates,NeuroStem progenitor cells form neurospheres that can be dissociatedfor further expansion or differentiation.

Genlantis provides two NeuroStem kits complete with optimized mediacomponents so you get the highest yield and viability out of each tubeof cells. Whether you are interested in testing for clonal yields, massproduction, grafting damaged brain tissue, or introducing specific genesinto animal brains, the NeuroStem Cells are your ideal choice for highquality and convenience. The following NeuroStem cell kits arecurrently available:

• Rat Hippocampal Progenitor Cells

• Rat Cortical Progenitor Cells

NeuroPure Rat Cortical Astrocytes

Neurospheres formed by NeuroStem Progenitor Cells

Live Primary Neuronal CellsLive Primary Neuronal CellsNeuroFECT™ Transfection ReagentThe Best Way to Deliver DNA into NeuronsNeuroFect™ Transfection Reagent is a novel cationic polymerformulated specifically for optimal transfection of primary neurons.During transfection, the polymer/DNA complexes (polyplexes) areendocytosed into the cells, where the polymer is biodegraded into non-toxic small molecules. The ability of the NeuroFect reagent tobiodegrade in vivo means cytotoxicity is reduced to a minimum.

With the NeuroFECT reagent, transfection efficiency of plasmid DNAreaches up to 65% in primary neurons. In contrast, the transfectionefficiencies with other commercially available reagents typically rangebetween 5% and 20%. Compared with electroporation, which requirescostly equipment and frequently results in cell death, the NeuroFectreagent is cost effective, easy to use, and offers maximum cell viability.

GenlantisGenlantis

Transfection of gWiz/GFP into Rat Hippocampal Neurons

Transfection of gWiz/GFP intoRat Cortical Neurons

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N100200 NeuroPure E18 Rat Hippocampal Neurons 2ml cells (~1 x 106) and 12 ml NeuroPure™ plating medium

N200200 NeuroPure E18 Rat Cortical Neurons 2 ml cells (~2 x 106) and 12 ml NeuroPure™ plating medium

N300200 NeuroPure P8 Rat Cerebellar Neurons 4 x 2 ml cells (~4 x 107) and 4 x 12 ml NeuroPure™ plating medium

N400200 NeuroPure E18 Rat Hypothalamus Neurons 1 pair of hypothalami and 12 ml NeuroPure™ plating medium

N500200 NeuroPure E18 Rat Striatal Neurons 1 pair of striata and 12 ml NeuroPure™ plating medium

N600200 NeuroPure E18 Rat Spinal Cord Neurons 1 spinal cord and 12 ml NeuroPure™ plating medium

N700200 NeuroPure E18 Rat Midbrain Neurons 1 midbrain and 12 ml NeuroPure™ plating medium

N800200 NeuroPure E18 Rat Hippocampal Astrocytes 2ml cells (1 rat) and 12 ml plating medium

N900200 NeuroPure E18 Rat Cortical Astrocytes 2 ml cells (1 rat) and 12 ml plating medium

N100300 NeuroStem Rat Hippocampal Progenitor Cells 2ml cells (~1 x 106), 12 ml NeuroPrep™ plating medium, 25 mg cellculture grade papain, and 12 ml NeuroStem™ plating medium

N200300 NeuroStem Rat cortical Progenitor Cells 2ml cells (~2 x 106), 12 ml NeuroPrep™ plating medium, 25 mg cellculture grade papain, and 12 ml NeuroStem™ plating medium

NM100100 NeuroPrep Media 100 ml

NM100200 Papain (cell culture grade) 100 mg

T800075 NeuroFECT Transfection Reagent 0.75 ml (75-300 reactions)

T800750 NeuroFECT Transfection Reagent 5 x 0.75 ml (375-1,500 reactions)

For research or laboratory use only. Not for use in diagnostic procedures.©2006 Genlantis, a Division of Gene Therapy Systems, Inc.

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ams biotechnology (europe) ltd

info@amsbio.com - www.amsbio.com

Available from ams biotechnology (europe) ltdinfo@amsbio.com - www.amsbio.com