logbook biochemistry intake sept 2015 edited

7/25/2019 Logbook Biochemistry Intake Sept 2015 Edited

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MEDICAL DEGREE MD 3101 LOG BOOK

METABOLISM AND NUTRITION (BIOCHEMISTRY)

SEPTEMBER 2015 INTAKE

No

.Pra!"a# To$"

Pa%& No.

1 Optimal enzyme activity 3

2 Determination of presence of sugar, ketonesbodies and protein in urine

9

3 Urinalysis: acroscopic, microscopic and urine

dipstick test

1!

! "stimation of total serum proteins and serum

albumin

21

# $enipuncture, intravenous %uid infusionprocedure and blood glucose test &glucometer'

2(

)ear 1* +iocemistry * -ractical anual * 1


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BIOCHEMISTRY LABORATORY SAFETY

.tudents /orking in a biocemistry laboratory must al/ays be a/are tat te

cemicals used are potentially to0ic, irritating and %ammable .tudents /o come tote laboratory session must ave a complete understanding of te laboratoryprocedures to carry out and be familiar /it bot te pysical and cemicalproperties of cemicals and reagents to be used +ecome familiar /it te locationand te use of standard safety features in te laboratory e laboratory is euipped/it 4re e0tinguisers, eye /ases, safety so/ers, fume oods and 4rst5aid kits6ny uestion regarding te use of tese facilities sould be addressed to yourinstructor

Sa'&! r#&* "+ %&+&ra#

1 "ating, drinking and smoking in te laboratory are strictly proibited

2 -roper attire must be /orn at all times7ear lab coat, protective glove and protective soes tat can protect your feet/en andling cemicals ie and pin long air, scarves and ead/ear out ofte /ay to avoid contact /it %ames or cemicals

3 Do not taste, smell, or touc any cemicals8reagents "$" put your noseover te bottle; 6void looking into te mout of any reaction vessel in /ic areaction is in progress

! 7ile eating a solution one sould make sure not to overeat it< terefore,vigorous mi0ing of te solution by saking or stirring is reuired e mout ofte glass/are containing te solution to be eated sould never be pointedto/ard anyone

# $olatile liuids and solids tat are to0ic or irritating sould be andled underfume oods

= eport ALLin>uries, allergies and8or medical problems to te lab instructor

? +efore leaving te laboratory, electrical euipment and gas burner sould be

turned o@


I, _______________________________________ , have read carefully and

understand the guidelines listed in BIOCHEMISTRY LBORTORY

S!ETY c"ntained "n this sheet# I agree t" act res$"nsi%ly and safely

in the la%"rat"ry at all ti&es#

S!,&+!-* *"%+a!r& Da!&


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PRACTICAL 1 OPTIMAL EN/YME ACTIITY

I+!ro,!"o+

.alivary amylase is a digestive enzyme secreted by te salivary glands Bt is responsible for starting te breakdo/n of te insoluble

polysaccaride Cstarc into soluble de0trins &oligosaccarides',maltose &disaccaride' and glucose &monosaccaride' in te mout sotat starc can be absorbed

Eomplete digestion of starc occurs in te small intestine by te actionof pancreatic amylase

.alivary amylase.tarc de0trin F maltose F glucose

O&!"&

1 o determine te e@ect of di@erent factors &suc as temperature and pGon enzyme activity'

2 o di@erentiate te common precipitation and color5canging in enzymee0periment

Ma!&r"a#* 4 R&a%&+!*

Ma!&r"a#* R&a%&+!*est tubes Distilled /ater+eaker H#I sodium cloride &aEl'

Bce 2I starc7ater bat & 3?JE and ?HJE' +enedict reagent+unsen burner 6lkaline solution

6cidic solutioneutral solution &normal saline'

Pro&,r&



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-reparing enzyme solutionB Bn a beaker, collect about 1 ml of your o/n CsalivaBB 6dd 9 ml of distilled /ater and =H ml of H#I sodium cloride solutionBBB gently mi0B$ Use tis Cenzyme solution to carry out te follo/ing four e0periments:

(a) E&! o' !&6$&ra!r& o+ !7& a!""! o' *a#"ar a6#a*&

Pr"+"$#&

.alivary amylase is protein in nature< te optimum temperature for its activityis 3?KE Bn e0treme temperatures &cold and eat', te activity of te enzymeis inibited &ig temperature causes denaturation of te protein structure ofte enzyme'

Pro&,r&

1 Let 3 test tubes and number tem C1, C2 M C3

2 -ut 2 ml of te previously prepared enzyme solution in eac of te treetest tubes

3 o eac test tube, add 2 ml of 2I starc solution and mi0 /ell

! Bmmediately put: test tube C1 on ice, test tube C2 in a 3?KE /ater bat test tube C3 in a ?HKE /ater bat

Neac for 2H minutes

# emove eac tube to room temperature= Earry out +enedicts test to ceck for te presence of te starc

ydrolysis product Cmaltose as follo/s: add 2 ml of +enedicts reagent to te enzyme5starc mi0ture in eac

tube put in a boiling /ater bat for # minutes

? 6fter #mins, place tem in a test tube rack( 7ait for fe/ minutes or until te colour cange is complete9 Observe te colour cangesRecord all results in the data table

)ear 1* +iocemistry * -ractical anual * !


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() E&! o' $H o+ !7& a!""! o' *a#"ar a6#a*&

Pr"+"$#&

e optimum pG for te activity of salivary amylase is te neutral pG &about?' Bn te acidic pG of te stomac, te activity of salivary amylase stops-ancreatic amylase, on te oter and, as an optimum activity in alkalinemedium

Pro&,r&

1 Let 3 test tubes and number tem C1, C2 M C3

2 -ut 2 ml of te previously prepared enzyme solution in eac of te tree

test tubes3 o eac test tube, add 2 ml of 2I starc solution and mi0 /ell

! Por:est tube C1, add 2 ml of acidic solutionest tube C2, add 2 ml neutral solutionest tube C3, add 2 ml alkaline solutionN-ut te 3 test tubes in a 3?KE /ater bat for 1H minutes

# emove eac tube to room temperature= Earry out +enedicts test to ceck for te presence of te starc

ydrolysis product Cmaltose as follo/s: 5add 2 ml of +enedicts reagent to te content of eac tube 5put in a boiling /ater bat for # minutes

logbook biochemistry intake sept 2015 edited

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