luciferase a. cengiz Öztireli. bioluminescence bioluminescence (living light) milky sea
TRANSCRIPT
LUCIFERASE
A. Cengiz Öztireli
BIOLUMINESCENCE
BIOLUMINESCENCE (LIVING LIGHT)Milky Sea
BIOLUMINESCENCE
Applications
BIOLUMINESCENCE
Sea AnemonesBacteria Dinoflagellates
BIOLUMINESCENCE
FIREFLYES (PHOTINUS)Attracting matesAttracting prey
LUCIFERASE
FIREFLY BACTERIA
BIOLUMINESCENCE
8 ATP → 1 Green Photon
Reaction:
LuciferinLuciferase + O2 Inter. P* P + hv
ATP
BIOLUMINESCENCE
Color P*
P* + F → F* + P Proteins around Structure
PHOTINUS PYRALIS (FIREFLY)
PHOTINUS PYRALIS LUCIFERASE
Luciferase + Luc-COOH
ATP
Luciferase: Luc-CO-AMP
O2
Luciferase: Luc=O*
PHOTINUS PYRALIS LUCIFERASE
N-Terminal
C-Terminal
Cleft
Structure and Functioning
CONCLUSIONS
THANK YOU