Isotonix® Vitamin D with K2

Market AmericaWorld Conference 2009

Elizabeth Rogers B.SnutraMetrix Product Manager

Isotonix® Vitamin D with K2

These statements have not been evaluated by the Food and Drug

Administration. This product is not intended to diagnose, treat, cure or

prevent any disease.

Vitamin D in the News

Vitamin D Deficiency

More than1 billion people worldwide do not consume adequate amounts of vitamin D

More than 50% of postmenopausal women taking medication for osteoporosis have suboptimal levels of vitamin D

Without adequate vitamin D, only 10-15% of dietary calcium is absorbed

Vitamin D

Fat-soluble vitamin Obtained in 3 ways:

› Dietary intake› Supplementation› Sunlight

Metabolically active form

Role of vitamin D

Promotes the proper synthesis and functioning of important proteins essential for proper calcium utilization

Supports the maintenance of bones Supports immune health Helps maintain cardiovascular health Helps maintain normal blood pressure

Dietary Sources of vitamin D

Salmon: 360 IUs Mackerel: 345 IUs Tuna: 200 IUs Sardines: 250 IUs Fortified Milk: 98 IUs

Why Vitamin K2?

Promotes normal calcium absorption Promotes healthy arteries Active form of vitamin K with highest

bioavailability Women with low bone density have

been found to be deficient in vitamin K Addresses the “calcium paradox”

Vitamin K1 vs. K2 Both are naturally occurring forms of

vitamin K Different chemical structures resulting in

different pharmacokinetic properties K1 is absorbed primarily in the liver

where at high doses it may interfere with anticoagulant medications

K2 is transported primarily to the bones and blood vessels

Only 10% of dietary vitamin K is in K2 form

Why take vitamins D and K as supplements?

Most people are vitamin D deficient or insufficient› Diet› Sun exposure

Very little vitamin K is stored in the body

These vitamins work together

Ingredients

Vitamin D3: 5,000 IU Vitamin K2: 45 mcg

Codes: 13071 (MA) 13972 (nutraMetrix) DC: $21.50 SR: $29.95 BV: 17

Benefits

Helps maintain bone health

Promotes normal bone mineral density

Promotes proper synthesis of proteins essential for proper calcium utilization

Helps maintain cardiovascular health

Promotes healthy arteries

Promotes elasticity of blood vessels

Helps maintain normal blood pressure

Promotes healthy cells

Supports immune health

Who would benefit?

Bone health Immune health Cardiovascular health Optimal nutrient absorption Optimizing overall health

How to Sell this Product Isotonic Vitamin D deficiency Calcium paradox

Code: 13071 (MA) 13972 (nutraMetrix) DC: $21.50 SR: $29.95 BV: 17

Bring your body to its best!

Distributor Cost: $21.50

Suggested Retail: $29.95

BV: 17

Product Code: 13071 (MA) 13972

(nutraMetrix)

BUY YOURS TODAY!!

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References Knapen M et al. Vitamin K2 supplementation improves hip bone geometry and bone strength indices in postmenopausal women.

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73(1):78-85, 2003. Hidaka T et al. Treatment for patients with postmenopausal osteoporosis who have been placed on HRT and show a decrease in

bone mineral density: effects of concomitant administration of vitamin K(2). Journal of Bone and Mineral Metabolism. 20(4):235-239, 2002.

Iwamoto J et al. Effects of vitamin K2 on osteoporosis. Current Pharmaceutical Design. 10(21):2557-2576, 2004. Iwamoto J et al. Treatment with vitamin D3 and/or vitamin K2 for postmenopausal osteoporosis. The Keio Journal of Medicine.

2003 Sep;52(3):147-50. Review. Neogi, T., et al. Low vitamin K status is associated with osteoarthritis in the hand and knee. Arthritis and Rheumatism.

54(4):1255-1261, 2006. Price P. Role of vitamin K-dependent proteins in bone metabolism. Annual Review of Nutrition. 8:565-583, 1988. Bekner K. The vitamin K-dependent carboxylase. Journal of Nutrition. 130(8):1877-1880, 2000. Nelsestuen G et al. Vitamin K-dependent proteins. Vitamins and Hormones. 58:355-389, 2000. Shearer M. Role of vitamin K and Gla proteins in the pathophysiology of osteoporosis and vascular calcification. Current Opinion

in Clinical Nutrition and Metabolic Care. 3(6):433-438, 2000. Habu D et al. Role of vitamin K2 in the development of hepatocellular carcinoma in women with viral cirrhosis of the liver. JAMA.

292(3):358-361, 2004. Yoshida T et al. Apoptosis induction of vitamin K2 in lung carcinoma cell lines: the possibility of vitamin K2 therapy for lung

cancer. International Journal of Oncology. 23(3):627-632, 2003. Lamson D and Plaza S. The anticancer effects of vitamin K. Alternative Medicine Review. 8(3):303-18, 2003. Yokoyama T et al. Combination of vitamin K2 plus imatinib mesylate enhances induction of apoptosis in small cell lung cancer

cell lines. International Journal of Oncology. 26(1):33-40, 2005. Chlebowski R et al. Vitamin K in the treatment of cancer. Cancer Treatment Review. 12:49-63, 1985. Lamson D et al. The anticancer effects of vitamin K. Alternative Medicine Review. 8(3):303-318, 2003.

References

Hitomi M et al. Antitumor effects of vitamins K1, K2 and K3 on hepatocellular carcinoma in vitro and in vivo. International Journal of Oncology. 26(3):713-720, 2005.

Nouso K et al. Regression of hepatocellular carcinoma during vitamin K administration. World Journal of Gastroenterology. 11(42):6722-6724, 2005.

Blackmore K et al. Vitamin D from dietary intake and sunlight exposure and the risk of hormone-receptor-defined breast cancer. American Journal of Epidemiology. 168(8):915-24, 2008.

Deluca H et al. Vitamin D: its role and uses in immunology. FASEB Journal. 15(14):2579-2585, 2001. Adorini L. Immunomodulatory effects of vitamin D receptor ligands in autoimmune diseases. International

Immunopharmacology. 2(7):1017-1028, 2002. Cantorna M et al. Mounting evidence for vitamin D as an environmental factor affecting autoimmune disease

prevalence. Experimental Biology and Medicine (Maywood). 229(11):1136-1142, 2004. Cantorna M. Vitamin D and autoimmunity: is vitamin D status an environmental factor affecting autoimmune

disease prevalence? Society for Experimental Biology and Medicine. 223:230-233, 2000. Garland C et al. The role of vitamin D in cancer prevention. American Journal of Public Health. 96(2):252-61,

2006. Giovannucci E et al. Prospective study of predictors of vitamin D status and cancer incidence and mortality in

men. Journal of the National Cancer Institute. 98(7):451-459, 2006. Holick, M. Vitamin D: Its role in cancer prevention and treatment. Progress in Biophysics and Molecular

Biology. 92(1):49-59, 2006. Gorham E et al. Vitamin D and prevention of colorectal cancer. Journal Steroid Biochemistry and Molecular

Biology. 97(1-2):179-94, 2005. Grant W et al. Reviews: A critical review of studies on vitamin D in relation to colorectal cancer. Nutrition and

Cancer. 48(2):115-123, 2004. Harris D et al. Vitamin D and colon carcinogenesis. Journal of Nutrition. 134(12):3463S-3471S, 2004. Hayes C et al. The immunological functions of the vitamin D endocrine system. Cellular and Molecular Biology. 49(2):277-300, 2003.

References Argiles A et al. Blood pressure is correlated with vitamin D(3) serum levels in dialysis patients. Blood Purification. 20(4):370-375,

2002. Kristal-Boneh E et al. Association of calcitriol and blood pressure in normotensive men. Hypertension. 30(5):1289-1294, 1997. Li Y et al. 1,25-Dihydroxyvitamin D(3) is a negative endocrine regulator of the renin-angiotensin system. Journal of Clinical

Investigation. 110(2):229-238, 2002. Li Y et al. Vitamin D regulation of the renin-angiotensin system. Journal of Cell Biochemistry. 88(2):327-331, 2003. Li Y et al. Vitamin D: a negative endocrine regulator of the renin-angiotensin system and blood pressure. Journal of Steroid

Biochemistry and Molecular Biology. 89-90(1-5):387-392, 2004. Pfeifer M et al. Effects of a short-term vitamin D(3) and calcium supplementation on blood pressure and parathyroid hormone

levels in elderly women. Journal of Clinical Endocrinology and Metabolism. 86(4):1633-1637, 2001. Sigmund C. Regulation of renin expression and blood pressure by vitamin D(3). Journal of Clinical Investigation. 110(2):155-156,

2002. Plaza S and Lamson D. Vitamin K2 in bone metabolism and osteoporosis. Alternative Medicine Reviews. 10(1):24-35, 2005. Review. Zitterman A et al. Low vitamin D status: a contributing factor in the pathogenesis of congestive heart failure? Journal of the

American College of Cardiology. 41(1):105-112, 2003. Schleithoff S et al. Vitamin D supplementation improves cytokine profiles in patients with congestive heart failure: a double-blind,

randomized, placebo-controlled trial. American Journal of Clinical Nutrition. 83(4):754-759, 2006. Argiles A et al. Blood pressure is correlated with vitamin D(3) serum levels in dialysis patients. Blood Purification. 20(4):370-375,

2002. Kristal-Boneh E et al. Association of calcitriol and blood pressure in normotensive men. Hypertension. 30(5):1289-1294, 1997. Li Y et al. 1,25-Dihydroxyvitamin D(3) is a negative endocrine regulator of the renin-angiotensin system. Journal of Clinical

Investigation. 110(2):229-238, 2002. Li Y et al. Vitamin D regulation of the renin-angiotensin system. Journal of Cell Biochemistry. 88(2):327-331, 2003. Li Y et al. Vitamin D: a negative endocrine regulator of the renin-angiotensin system and blood pressure. Journal of Steroid

Biochemistry and Molecular Biology. 89-90(1-5):387-392, 2004. Pfeifer M et al. Effects of a short-term vitamin D(3) and calcium supplementation on blood pressure and parathyroid hormone

levels in elderly women. Journal of Clinical Endocrinology and Metabolism. 86(4):1633-1637, 2001. Sigmund C. Regulation of renin expression and blood pressure by vitamin D(3). Journal of Clinical Investigation. 110(2):155-156,

2002.