Measuring Amount of Bacteria by

Index of The Most Probable Number

Menu1 •Principle

2 •Equipments

3 •Material

4 •Procedure

5 •Work Chart

6 •Conclusion

Principle

– In determining amount of bacteria, solid or liquid sample is for diluting in 10-1 until 10-3 . Inoculation with TSB as media, and then will inkubating in 370 C for 24 hour. In every diluting also have to observeted carefully the muddy of solution, and check the result we get dapand on the The Most Logical Number Table

EquipmentsSterill of Erlenmeyer

Ulir tube

Serological pipette 1 ml

Serological pipette 5 ml

Serological pipette 10 ml

Colony counter

Spirtus burner

Plastic wash bottle

Bulb

• The Material which need:1. BPW (Bacto Peptone Water)2. LF3. Sample Solid4. TSB media (Tryptic Soy Broth)5. Rinse alcohol

Materials

Add 9 ml BPW

solution into tube I, II, III,

IV

Pipette 1 ml sample, get into tube I,

homogenize stick label10-

1 Pipette 1 ml sample from

tube I, get into three of

every ulir tube

Pipette 1 ml sample from

tube I, get into tube II.

Homogenize (stick label 10-

2)Pipette 1 ml

sample from tube II, get into three of

every ulir tube

Pipette 1 ml sample from tube II, get

into tube III. Homogenize

(stick label 10-

3)Do an

effectivity test

Do an sterillity test

Incubate all of ulir tube in

inkubator 370

Procedure

Work Chart

Conclusion

THANKS FOR YOUR KIND ATTENTION

: DD

Presented by:

Husnun Hanifah

Isbir Farhan

Mochammad Fajar

Nadia Nahla K

Nuruzzahra A