mek8222 trm b

Automated Hematology Analyzer

MEK-8222J/K

0634-002048B

If you have any comments or suggestionson this manual, please contact us at:www.nihonkohden.com

Copyright NoticeThe entire contents of this manual are copyrighted by Nihon Kohden. All rights are reserved. No part of thisdocument may be reproduced, stored, or transmitted in any form or by any means (electronic, mechanical,photocopied, recorded, or otherwise) without the prior written permission of Nihon Kohden.

N.1Technical Reference Manual MEK-8222

After signing this non-disclosure agreement or equivalent, you can use the technical reference manual.

Non-Disclosure Agreement (Sample)

THIS AGREEMENT between __________________________ (COMPANY) and Nihon Kohden Corporation (NK)

effective from the date stated below by COMPANY, shall govern the conditions of disclosure of the Technical

Reference Manual and other confidential technical information given to COMPANY by NK relating to Nihon

Kohden hematology analyzer ____________________(collectively "Confidential Information").

COMPANY hereby agrees not to disclose Confidential Information to any other party or copy the Confidential

Information without the express written consent of NK. Upon request of NK, COMPANY shall immediately return

the Technical Reference Manual, including all copies, to NK.

THIS AGREEMENT shall not apply to the following information.

(a) Publicly known before or after disclosure by NK through no fault of COMPANY.

(b) In the possession of COMPANY before disclosure by NK.

(c) Acquired by COMPANY from a third party having an independent right to disclose the information.

If COMPANY causes damage to NK against THIS AGREEMENT, COMPANY shall make compensation for the

damage.

When any discrepancy occurs in the interpretation of THIS AGREEMENT or when any discrepancy occurs between

COMPANY and NK in regard to matters not stipulated in THIS AGREEMENT, COMPANY and NK shall discuss

the discrepancy amicably in order to reach a solution in good faith.

Two copies of THIS AGREEMENT shall be made and signed by COMPANY and NK. COMPANY and NK shall

each have one copy of THIS AGREEMENT.

COMPANY NK

Date: ______________________________________ Date: _____________________________________

Company: __________________________________ Nihon Kohden Corporation

Address: ___________________________________ 1-31-4, Nishiochiai, Shinjuku-ku, Tokyo

___________________________________________

Signature: __________________________________ Signature: _________________________________

Signee's name in block letters: ___________________________________________

CONTENTS

Technical Reference Manual MEK-8222 C.1

ContentsSection 1 General .................................................................................. 1C.1

Connection Cable List ..........................................................................................................1.1Connection and Wiring Diagram ............................................................................................1.3Hydraulic System Diagram ...................................................................................................1.4Tube List ...............................................................................................................................1.5

Section 2 Replaceable Parts List......................................................... 2C.1Units and Boards ..................................................................................................................2.2Chassis ................................................................................................................................2.4MO-820V Laser Optical Unit .................................................................................................2.6MF-820V Flowcell Unit .........................................................................................................2.7JQ-820V Inlet/Outlet Unit .....................................................................................................2.8JQ-821V Left Side Piping Unit ..............................................................................................2.9JQ-822V Center Piping Unit ............................................................................................... 2.10JQ-823V 4-channel Liquid Sensor Unit ............................................................................... 2.11PV-820V Display Unit ......................................................................................................... 2.12ZY-820V Warmer Piping Unit ............................................................................................... 2.13ZK-821V/VG CCD Bar Code Reader Unit (Option) .............................................................. 2.14MB-820V Sub Bath Unit ..................................................................................................... 2.15MC-820V CBC Measuring Unit ........................................................................................... 2.16MC-821V Flow Cytometry Piping Unit ................................................................................ 2.18MP-520V Pump Unit ........................................................................................................... 2.20MP-821V Sample Pump Unit .............................................................................................. 2.21MP-820V Complex Pump Unit ............................................................................................ 2.22MP-822V Sheath Pump Unit ............................................................................................... 2.24MS-820V Closed Sampler Unit ........................................................................................... 2.26MS-821V Open Sampler Unit ............................................................................................. 2.28MS-822V Table Unit ............................................................................................................ 2.29PV-820V Display Unit ......................................................................................................... 2.30ZY-820V Warmer Piping Unit ............................................................................................... 2.31ZK-821V/VG CCD Bar Code Reader Unit (Option) .............................................................. 2.32

CONTENTS

C.2 Technical Reference Manual MEK-8222

This page is intentionally left blank.

Technical Reference Manual MEK-8222 i

EMC RELATED CAUTIONThis equipment and/or system complies with the International Standard EN61326-1 for electromagneticcompatibility for medical electrical equipment and/or system. However, an electromagnetic environment thatexceeds the limits or levels stipulated in the EN61326-1, can cause harmful interference to the equipmentand/or system or cause the equipment and/or system to fail to perform its intended function or degrade itsintended performance. Therefore, during the operation of the equipment and/or system, if there is anyundesired deviation from its intended operational performance, you must avoid, identify and resolve theadverse electromagnetic effect before continuing to use the equipment and/or system.

The following describes some common interference sources and remedial actions:

1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station orcellular phone:Install the equipment and/or system at another location if it is interfered with by an emitter source suchas an authorized radio station. Keep the emitter source such as cellular phone away from the equipmentand/or system.

2. Radio-frequency interference from other equipment through the AC power supply of the equipment and/or system:Identify the cause of this interference and if possible remove this interference source. If this is notpossible, use a different power supply.

3. Effect of direct or indirect electrostatic discharge:Make sure all users and patients in contact with the equipment and/or system are free from direct orindirect electrostatic energy before using it. A humid room can help lessen this problem.

4. Electromagnetic interference with any radio wave receiver such as radio or television:If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/orsystem as far as possible from the radio wave receiver.

If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden Corporationsubsidiary or distributor for additional suggestions.

The CE mark is a protected conformity mark of the European Community. The products herewith complywith the requirements of the IVD Directive 98/79/EEC.

This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class BEQUIPMENT is equipment suitable for use in domestic establishments and in establishments directlyconnected to a low voltage power supply network which supplies buildings used for domestic purposes.

ii Technical Reference Manual MEK-8222


Technical Reference Manual MEK-8222 iii

Conventions Used in this Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.

WARNINGA warning alerts the user to the possible injury or death associated with the use or misuse of theinstrument.

CAUTIONA caution alerts the user to possible injury or problems with the instrument associated with its use ormisuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to otherproperty.

NOTEA note provides specific information, in the form of recommendations, prerequirements, alternative methodsor supplemental information.

iv Technical Reference Manual MEK-8222

Symbol Description Symbol Description

Main power lamp HEMOLYNAC3 (hemolysing reagent)

On only for part of the equipment HEMOLYNAC5 (hemolysing reagent)

Off only for part of the equipment WASTE

Laser ON Hand positions for carrying the instrument

Reset RS-232C socket

Manual USB socket

Clean IVD conformity

Eject Fuse

Start AC power off (Disconnection from the mains)

Emergency AC power on (Connection to the mains)

Do not touch the sample rack during measurement Alternating current

Inlet Equipotential terminal

Outlet Year of manufacture

Attention, consult operator's manual Serial number

ISOTONAC3 (diluent)

CLEANAC (detergent)

CLEANAC3 (detergent)

The CE mark is a protected conformity mark of the European Community. The products herewith comply with the requirements of the IVD Directive 98/79/EEC.

Explanations of the Symbols in this Manual and InstrumentThe following symbols found in this manual/instrument bear the respective descriptions as given.

On panel

IVD

Technical Reference Manual MEK-8222 v

On screen and recorded data

Symbol Description Symbol Description

Beside WBC or RBC measured value: Sample error Beside HGB measured value: Dirty measurement baths

Beside HGB measured value: HGB circuit error

Beside WBC measured value: Poor hemolyzation Beside HGB measured value: HGB voltage adjustment error

Beside WBC or PLT measured value: Platelet coagulation

C

Technical Reference Manual MEK-8222 1C.1

Section 1 General

Connection Cable List .........................................................................................................1.1Connection and Wiring Diagram ...........................................................................................1.3Hydraulic System Diagram ..................................................................................................1.4Tube List ..............................................................................................................................1.5

1. GENERAL

Technical Reference Manual MEK-8222 1.1

Connection Cable List

Cable No. Unit NK code Cable model or description Cable ends CNP 306 JQ-820V 261753 HKP-6F02-3BT 4WAY LIQUID SENSOR board 2 valves

CNP 238 JQ-821V 374391 MFC20HF DRIVER2 board 7 valves

CNA 205 JQ-822V 622489B PHR-4/HKP-8F02-4BT (W170) DRIVER1 board LIQUID LEVEL SENSOR board

CNA 206 JQ-823V 620393C 1-171822-2 (W300) DRIVER1 board 4WAY LIQUID SENSOR board

CNA 220 620384 PHDR-12VS/ZHR6 (W280) DRIVER4 board 2 motors

CNA 221 MB-820V 620366 PHDR-10VS/171822-3 (W280) DRIVER4 board 2 sensors

CNA 239 620321A 171822-3/1-0480435-0W390 DRIVER2 board Manual count switch

CNP 240 374382 MFC-16HF DRIVER2 board 7 valves CNA 241 620339A 171822-3/(W70) PRE AMP board Electrode for RBC CNA 242 620339A 171822-3/(W70) PRE AMP board Electrode for WBC CNA 243 620348A 171822-6/M63M83-07 (W70) PRE AMP board Phototransistor of RBC manometer CNA 244 536582 M63M83-04 (S80) PRE AMP board LED of RBC mamometer

CNA 245 620348A 171822-6/M63M83-07 (W70) PRE AMP board Phototransistor of WBC manometer CNA 246 536582 M63M83-04 (S80) PRE AMP board LED of WBC mamometer

CNA 247

MC-820V

620357A M63M83-08/M63M83-04 (W140) PRE AMP board HGB AMP and HGB LED boards

CNP 204 MC-821V 374382 MFC16HF DRIVER1 board Valve

CNA 103 MO-820V 529652A MFC16/M63M83/171822-4 (W690) MASTER board MO preamp board set and MO LD DRIVER board

CNA 240 MP-520V DRIVER1 board 5-part WBC pump unit CNA 236 MP-520V DRIVER1 board RBC pump unit CNA 237 MP-520V DRIVER1 board WBC pump unit CNP 222 374391 MFC-20HF DRIVER4 board 2 motors and 4 valves CNA 223 620099C 1-171822-0 (W580) DRIVER4 board MD CONNECTION board CNA 224

MP-820V 620081C 171822-4 (W300) DRIVER4 board MP CONNECTION board

CNP 201 374408 MFC10HF DRIVER1 board 2 valves and motor CNA 202

MP-821V 620107B 171822-8/171822-3 (W330) DRIVER1 board 2 sensors

CNA 105 619011A PHDR-28VS (W230) MASTER board SHEATH CONTROL board CNP 207 374382 MFC16HF SHEATH CONTROL board 5 valves and motor CNA 208

MP-822V 619002 PHR-4 (W50) SHEATH CONTROL board LIQUID LEVEL SENSOR board

CNA 014 619992 VHR-6N (W370) SLAVE MS board DRIVER3 board CNA 109 620009 HIF3BA-50D-AC-27 SLAVE MS board DRIVER3 board CNA 227 620018C MFC26HF/PHR-11/(W260) DRIVER3 board 3 motors and 3 valves CNP 227 374408 MFC10HF DRIVER3 board 2 motors

CNA 228 620036B PHDR-20VS/171822-6 (W400) DRIVER3 board 3 sensors and 3POS SENSOR board

CNA 229

MS-820V

620045A PHDR-24VS/171822-3 (W350) DRIVER3 board 6 sensors

CNP 226 374382 MFC16HF DRIVER4 board 2 motors and valve

CNA 225 MS-821V 620054B PHDR-18VS/171822-3 (W290) DRIVER4 board 3 sensors and SENSOR board

CNA 015 620125A VHR-6N (W500) SLAVE MS board DRIVER5 board CNA 110 620116A HIF3BA-60D-AA-47 SLAVE MS board DRIVER5 board CNA 230 536591 171822-8/PHR-11 (W85) DRIVER5 board Motor CNP 231 149287 171822-7 DRIVER5 board Motor CNA 232 620134A 171822-6 (W260) DRIVER5 board SENSOR3 board CNA 233

MS-822V

620143A HIF3BA-16D-CC-27 DRIVER5 board TABLE SENSOR board

1. GENERAL

1.2 Technical Reference Manual MEK-8222

Cable No. Unit NK code Cable model or description Cable ends CNA 209 601716 Dedicated cable for touch screen LCD CONTROL board Touch screen

CNA 210 622444 PHDR-16VS (W430) LCD CONTROL board MANUAL KEY board CNA 211 622453A PHDR-12VS (W330) LCD CONTROL board RACK KEY board CNA 212 622462 DF19G-30S-1C (W40) LCD CONTROL board LCD CNA 213 601707 Dedicated cable for LCD LCD CONTROL board LCD

CNA 214 575726A 51021-0800/PHDR-10VS (W100) LCD CONTROL board Inverter board

CNA 215 622471A PHR-7/XHP-7 (W65) LCD CONTROL board Inverter board

PV-820V

539463 NA19002-4220 (FLCV-08) Inverter board

CNA 113 620268A PHDR-20VS/MFC-20HF (W135) MASTER board INTERFACE board

CNA 216 622515A PHDR-20VS/JEC-9P/-25S (W60) INTERFACE board Rear panel

CNA 301 620277A PHDR-10VS (W60) MASTER board SLAVE MS board CNA 302 620286 PHDR-10VS (W430) MASTER board SLAVE CBC board CNA 303 620277A PHDR-10VS (W60) SLAVE MS board SLAVE CBC board CNA 304 622524A 171822-4 (S130) 4WAY LIQUID SENSOR board Emergency switch

374426 LP2S-17S-889 Emergency switch CNA 305 622533A 171822-2 (S100) 4WAY LIQUID SENSOR board Laser key switch

374417 SK-12BAS1 Laser key switch CNA 008 536617 VHR-6N (W200) MASTER board DRIVER1 board CNA 102 620446A HIF3BA-40D-AC-17 MASTER board DRIVER1 board CNA 010 620401 VHR-6N (W290) SLAVE CBC board DRIVER2 board CNA 011 620428 VHR-2N (W200) SLAVE CBC board DRIVER2 board CNA 106 620455 HIF3BA-50D-AC-17 SLAVE CBC board DRIVER2 board CNA 013 620125A VHR-6N (W500) SLAVE MS board DRIVER4 board CNA 108 620464B HIF3BA-64D-AC-57 SLAVE MS board DRIVER4 board CNA 009 622426A VHR-5N (W1050) MASTER board LCD CONTROL board CNA 104 622435B HIF3BA-60-AC-117 MASTER board LCD CONTROL board CNA 012 620303A VHR-8N (W400) SLAVE CBC board PRE AMP board CNA 107

RK-820V

620312B HIF3BA-40D-AA-47 SLAVE CBC board PRE AMP board

CNA 001 419272 41729 L=130 AC inlet socket with main power switch Ground termimnal

534486B T-534486A AC inlet socket with main power switch Power transformer for 100 V AC



539561 L-41-20 POWER board High frequency reduction coil CNA 002 620197A VHR-10N (W100) POWER board MASTER board CNA 003 620241 VHR-3N (W360) POWER board MASTER board CNA 005 620223A VHR-10N (W370) POWER board SLAVE CBC board CNA 006 620214A VHR-7N (W500) POWER board SLAVE CBC board CNA 007 620223A VHR-10N (W370) POWER board SLAVE MS board CNA 101

SC-820V

620232 PHR-6 (W90) POWER board MASTER board

CNA 217 ZK-820V 531489 Dedicated cable for barcode reader Rear panel CCD barcode reader

CNA 234 620072 PHDR-12VS/ZHR-6 (w250) DRIVER5 board Motor and sensor CNP 235 620161 PHDR-10VS DRIVER5 board Bar-code reader

ZK-821V

531578 BL-185 DRIVER5 board Bar-code reader

CNA 004 622498 VHR-2N (W600) POWER board WARMER CONTROL board CNA 112 622506A PHDR-12VS (W660) MASTER board WARMER CONTROL board

CNA 218 536983 Dedicated cable for container heater WARMER CONTROL board Container heater

CNP 219

ZY-820V

374408 MFC10HF DRIVER4 board 2 valves

CNA 241 278111 Dedicated cable for card printer Rear panel Card printer

CNA 242 584351A DSUB-HAB13-FC07 (L=0.7m) Rear panel Personal computer

CNA 243 514026A DSUB-HAB13-FC20 (L=2.0m) Rear panel Personal computer

1. GENERAL


Connection and Wiring Diagram

Display unit PV-820V

Inverter board

POWER board UT-7160

DRIVER5 boardUT-7156

Left side piping unitJQ-821V

Connection and Wiring Diagram

H8SCPU

CCD barcodereader ZK-822V

(Option)

Power supply unit SC-820VK

Sheath pump unit MP-822V

SHEATH CONTROL boardUT-7158

Flow cytometry piping unitMC-821V 2MV12MV

2

PMV7

2MV3

PMV5

2MV4

3MV8

Laser optical unit MO-820V

MO preamp board set UT-7172

LD DRIVER board UT-7176

Touch screen MANUAL KEY board

INTERFACE board UT-7161

3MV1

3MV2

3MV1

3MV4

3MV5

3MV6

3MV7

WARMER CONTROL boardUT-7159

CBC measuring unit MC-820V

MANOMETER

PTR board

2MV1

2MV2

2MV3

2MV4

3MV5

2MV6

3MV7

Pump unit MP-520V (5-part WBC)MTPH Position Sensor

Sample pump unit MP-821V

Open sampler unit MS-821V

3MV1

3MV2

Table unitMS-822V

4WAY LIQUID SENSOR board


3MV2

3MV3

Complex pump unitMP-820V


MTPH

MTPH

MTPX

3MV1

3MV2

3MV3

MTPX

3MV4

3MV1


3MV1

3MV2

3MV3

TABLE SENSOR board UT-7157

Pi

RACK KEY boardSwitchLED

EjectStart

EmergencyClean

USB

LCD CONTROL board

MTRSPG

MTWSPG

LIQUID LEVELSENSOR board

UT-7171

LIQUID LEVEL SENSOR boardUT-7171

Laser switch Emergency stop switch

MD CONNECTION board

MD SETSW1

MT1PX

MT2PX

MT1PX

MT2DC

MT1PK5

MT2PX

MT3PK5

MT1PK5

MT2PX

MTSPG

2MV

2MV1

PK243

RS-232CDSUB 9P

RS-232CDSUB 25P

Data ManagementProgram for PC

Qi-820V (Option)

Handy BarcodeReader Unit ZY-820V (Option)

Impact Printer WA-711V(Option) or locally avialableprinter LQ-300, LQ-300+,

LX-300+

Card PrinterWA-460V (Option)

Thermal PrinterWA-820V (Option)

2MV1

2MV2

2MV3

2MV4

MT1DC

2MV5

50P

LED board

HGBAMPboard

HGBLED

Pump unit MP-520V (RBC)

MFC20P

MFC16P

MFC10P

VH6P

PHD12P

PHD10P

VH10P

VH10P VH10P

VH 7P VH 7PVH10P

HIF60P

VH6P

VH6P

SENSOR3 boardUT-7086

AMP6P

60P

VH-6P

AMP8P AMP7P

HIF-16PPHD12

BL-185 PHD10VH6P

PHD20P

PHD24P

UT-7155

2MV6

POSCODE0CODE1

UD1

UD0

UD4

UD5

UD0

UD1

UD2

UD3

CN201

CN203

CN202

HIF50PCN101

CN001

PiPO

S0

MFC16P

PHD28PCN101

CN201

PHD28P

PiPO

S1Pi

POS2

PiPO

S3Pi

POS4

PiPO

S5Pi

POS6

PiPO

S7Pi

POS8

PiPO

S9Pi

POS10

PiPO

S11

CN001VH6P

CN201CN202

CN203

ORIGINENCODEMD SETSW2

RL0,1,2,3

SL0 SL1 AMP10P

UD1Position sensor UD0

AMP4PCN205

CN206

CN207

CN208

SENSOR board UT-7065POSCODE0CODE1

UD0

UD1

50P

HIF50P

HIF50PCN101CN001

VH6P

HIF-60PCN101

CN001

CN201 CN202

CN102 CN203

Position sensor POSCODE0CODE1CODE2CN204CN205

CN204

UD2

VH5P HIF-60P

VH5P

DC lineAC line

VH6P

Serial communication Iine

VH10PVH3P

PHK6PVH10PVH3P

PHK6P

MFC16P

PHD12P

AMP2P AMP4P AMP12P

MFC10P

MFC16P

AMP8P

AMP9P

UD1

UD0

SEN

PHD12P

SEN SEN

VH6P

CN002VH-2P

VH2P40P

CN001

HIF40P HIF40P

VH8P VH8P

CN001

CN002

CN003

CN101

CN004

CN005

CN102

SLAVE MS boardUT-7148

PHD10PCN302PHD10P

CN301PHD10P

CN301 PHD10P

SLAVE CBC boardUT-7147

PHD10PCN301

CN302

CN001

HIF50PCN102

CN003

CN002

64P

HIF64P

HIF-64PCN101

CN101

CN004

CN103

MFC-26P

PHD10PCN302

HKP6PAMP12PCN201

CN202 CN203

CN101

Up

CN005

CN101

CN003

CN004

CN009

CN007

CN006

CN202

CN201

CN203

CN204

CN205

CN206

VH6P

40P

CN001

HIF40P

CN101

AMP2P

HIF40P

CN001

CN002

CN101

CN104CN004

CN103CN003

CN102

CN105

CN303

CN106CN107

MASTER boardUT-7146

VH2P

CN001 CN101

CN102

PHD12PCN103

CN301

LCD unit

DF-30PCN204SM04P

CN203SFW15R

PHD16PPHK7P PHD10P

CN205 CN202

172034-1CN001

350428-1CN002

AMP9PCN203AMP9PCN204

CN102

AMP3PCN103

MFC16PCN201MFC20PCN202

3MV1

CNA224

CNA223AMP10PAMP4P

CNA012

CNA107

CNA211

CNA210

CNA239AMP3P

CN204

MFC-10P

CNA227

CNP227

CNA009 CNA104

HKP8P

CNA008 CNA102

CNA206

CNA240

CNA219

CNA108 CNA013

CNA014

CNA109

VH2P

HIF-60P

CNA216

CNA113PHD20P

CNA105

CNP207

CNA208 PHKS4PCN202

CNA305 CNA304

CNA302

CNA301

CNA302CNA303 CNA303

CNA005

CNA006CNA007

CNA101CNA002CNA003

CNA103

CNA205

CNP238

CNP306

CNA2

09

CNA2

12

CNA2

13

CNA2

14

CNA2

15

CNA004

CNA112PHR5 CNA218

CNA228

CNA227

CNA229

CNA220

CNA221CNP222

CNP225

CNA226

CNA110

CNA015

CNP231CNA230

CNP235

CNA234

CNA232

CNA233

CNA010

CNA011CNA106

CNA237

CNA236

CNA202

CNP201

CNA242CNA243

CNA241CNA217

CNP240CNP238

CNA247WBC RBC

CNA243CNA246

CNA242

PTR board

CNA242CNA245

CNA241

PHD18P

EjectStart

EmergencyClean

LED SwitchMain powerPowerLaserOperation indicatorManual mode

Power keyManual mode keyReset key

H8SCPU

4-Channel liquid sensor unit JQ-823V

UT-7179

Depressure needle

Upper Position

Position sensor

Lower Position

Position sensor

Pump unit MP-520V (WBC)

Manualcountswitch

PRE AMP board UT-7143

Spare Turn Up and Down

Upper position

Lower position

Middle position

Down Position sensorPosition sensorPosition sensor

Position sensorPosition sensorPosition sensor

Position SensorPosition SensorPosition Sensor

3POS SENSOR board UT-7185

Closed sampler unit MS-820V

Warmer unit ZY-820V

Containerwith

heater

for MP for MD (Diluter)

Up and Down Turn

TurnUp and Down(Needle) Up and Down(Nozzle)

Right and Left Back and Forth

Upper position

Upper position

Lower position

Lower position

Manual sampling nozzle

DRIVER4 board

H8SCPU

Center piping unitJQ-822V

Inlet/outlet unitJQ-820V

RBC WBC

LED board

LED board

LED board

board

Apertu

reca

p

Apertu

reca

p

WBCRBC

Sub bath unit MB-820V

Position sensorPosition sensorPosition sensor

Depressure needle Middle position

Upper position

Middle position

Lower position

Sample tube

For mixing

Sampling nozzle

Sampling nozzle

Sampling nozzle

Depressure needle

2

MANOMETER

MP CONNECTION board

1. GENERAL


Hydraulic System Diagram

1. GENERAL


In

dex

Mod

el N

umbe

rRe

mar

ks1

YK

-C

O09

02

YK

-C

O09

03

YK

-C

O09

04

YK

-C

O08

55

YK

-B

O25

06

YK

-D

O16

07

YK

-A

D21

08

YK

-A

C35

09

YK

-A

D17

010

YK

-A

C30

011

YK

-A

D30

012

YK

-A

C28

013

YK

-A

C20

0

In

dex

Mod

el N

umbe

rRe

mar

ks1

YK

-C

O08

52

YK

-C

O12

03

YK

-B

D19

04

YK

-A

D32

0

In

dex

Mod

el N

umbe

rRe

mar

ks1

YK

-D

O30

52

YK

-C

O30

53

YK

-D

O27

04

YK

-C

O33

05

YK

-A

C40

06

YK

-B

C45

07

YK

-C

D17

0

In

dex

Mod

el N

umbe

rRe

mar

ksY

K-

0000

3Tu

be w

ith g

rom

met

(615

mm

long

)

Tube List

Not

e: T

o u

se 7

.5 m

tube

, cut

it in

the

optim

al le

ngth

.

In

dex

Mod

el N

umbe

rRe

mar

ks1

YK

-C

O13

02

YK

-B

D12

03

YK

-B

O06

54

YK

-A

O18

05

YK

-D

O15

06

YK

-B

C25

07

YK

-B

C32

58

YK

-C

O32

09

YK

-D

O11

010

YK

-D

O16

011

YK

-D

O21

012

5591

4730

7.5

m tu

be13

5591

4755

7.5

m tu

be14

5591

4710

07.

5 m

tube

1555

9147

230

7.5

m tu

be16

5341

0221

07.

5 m

tube

1753

4102

230

7.5

m tu

be18

YK

-C

L30

0

Inde

xM

odel

Num

ber

Rem

arks

1Y

K-

CO

180

2Y

K-

CO

180

3Y

K-

0000

2Tu

be w

ith g

roun

d te

rmin

al (1

80m

m lo

ng)

4Y

K-

OO

090

5Y

K-

DO

160

6Y

K-

DO

160

7Y

K-

CC

140

8Y

K-

CC

150

9Y

K-

CD

640

10Y

K-

CD

280

11Y

K-

CO

150

12Y

K-

CO

150

13Y

K-

CD

180

14Y

K-

CD

200

15Y

K-

CO

090

16Y

K-

CO

090

17Y

K-

CO

050

18Y

K-

0000

1Tu

be w

ith c

apac

itor a

nd g

roun

dte

rmin

al (2

40 m

m lo

ng)

19Y

K-

A

270

20Y

K-

AO

140

In

dex

Mod

el N

umbe

r

Rem

arks

1

YK

- O

O

085

2

YK

- O

O

084

3

YK

- C

O

095

4

YK

- O

O

195

5

YK

- A

O

400

6

YK

- C

O

140

7

YK

- C

O

085

8

YK

- O

O

125

9

YK

- D

O

080

10

Y

K-

CO

23

5

11

YK

- O

O

090

12

Y

K-

DO

09

0

13

YK

- D

D

100

14

Y

K-

AC

15

0

15

YK

- A

O

300

16

Y

K-

DO

08

0

17

YK

- C

O

080

18

L=

55

19

YZ-

0282

Pi

nch

valv

e tu

be se

t Q

ty 2

eac

h L=

60

1. GENERAL


Inde

xM

odel

Num

ber

Rem

arks

1Y

K-

FO13

02

YK

-A

O15

03

YK

-A

O12

04

YK

-FO

075

5Y

K-

DF

110

6Y

K-

CF

120

7Y

K-

FO16

08

YK

-FO

170

9Y

K-

BF

140

10Y

K-

BO

180

11Y

K-

BO

140

12Y

K-

OO

120

13Y

K-

FO14

014

YK

-B

O15

015

YK

-A

O16

016

YK

-A

O13

017

YK

-B

O23

018

YK

-C

D13

019

YK

-A

C12

020

YK

-A

L50

0

Inde

xM

odel

Num

ber

Rem

arks

1Y

K-

CD

150

2Y

K-

CC10

03

YK

-A

C33

04

YK

-D

K33

05

YK

-B

C31

06

YK

-D

O03

07

YK

-CC

100

8Y

K-

DK

320

9Y

K-

CC10

010

YK

-B

C29

0

Inde

xM

odel

Num

ber

Rem

arks

1Y

K-

AC

150

2Y

K-

AO

680

3Y

K-

BO

530

4Y

K-

BO

660

5Y

K-

BO

700

6Y

K-

CO20

07

YK

-D

O13

08

YK

-O

O11

09

YK

-O

O12

010

YK

-O

O12

011

YK

-O

O40

012

YK

-O

O45

0

Inde

xM

odel

Num

ber

Rem

arks

1Y

K-

CO19

02

YK

-A

O17

03

YK

-A

O17

04

YK

-A

O17

0

In

dex

Mod

el N

umbe

rR

emar

ks1

YK

-O

O21

02

YK

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110

3Y

K-

BC

300

4Y

K-

CD13

05

YK

-A

C16

06

YK

-A

D35

0

In

dex

Mod

el N

umbe

rR

emar

ksA

YK

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B48

0B

YK

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A35

0C

YK

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O40

0D

YK

-A

O48

0E

YK

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O48

0F

YK

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O95

0G

YK

-B

O75

0H

YK

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C52

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YK

-A

C50

0J

YK

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D90

0K

YK

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K60

0L

YK

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D75

0M

YK

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O10

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YK

-A

D75

0O

5326

3947

07.

5 m

tube

Stan

dard

Acc

esso

ryY

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0000

4Tu

be fo

r HEM

OLY

NA

C3

(400

mm

long

)Y

K-

0000

5Tu

be fo

r HEM

OLY

NA

C5 (4

00 m

m lo

ng)

Not

e: T

o u

se 7

.5 m

tube

or 1

00 m

tube

,cu

t it

in th

e op

timal

leng

th.

In

dex

Mod

el N

umbe

rRe

mar

ks1

YK

-A

C47

02

5326

3920

07.

5 m

tube

353

2639

200

7.5

m tu

be4

YZ-

0293

Silic

one

tube

set (

L=30

, Qty

3)

Technical Reference Manual MEK-8222 2C.1

Section 2 Replaceable Parts List

Units and Boards .................................................................................................................2.2Chassis ...............................................................................................................................2.4JQ-820V Inlet/Outlet Unit ....................................................................................................2.6JQ-821V Left Side Piping Unit .............................................................................................2.7JQ-822V Center Piping Unit ................................................................................................2.8JQ-823V 4-channel Liquid Sensor Unit ................................................................................2.9MB-820V Sub Bath Unit .................................................................................................... 2.10MC-820V CBC Measuring Unit .......................................................................................... 2.12MC-821V Flow Cytometry Piping Unit ............................................................................... 2.14MF-820V Flow Cell Unit ..................................................................................................... 2.16MO-820V Laser Optical Unit .............................................................................................. 2.17MP-520V Pump Unit .......................................................................................................... 2.18MP-820V Complex Pump Unit ........................................................................................... 2.20MP-821V Sample Pump Unit ............................................................................................. 2.22MP-822V Sheath Pump Unit .............................................................................................. 2.24MS-820V Closed Sampler Unit .......................................................................................... 2.26MS-821V Open Sampler Unit ............................................................................................ 2.28MS-822V Table Unit ........................................................................................................... 2.29PV-820V Display Unit ........................................................................................................ 2.30ZY-820V Warmer Piping Unit .............................................................................................. 2.31ZK-821V/VG CCD Bar Code Reader Unit (Option) ............................................................. 2.32

2. REPLACEABLE PARTS LIST


When ordering parts or accessories from your nearest Nihon Kohden Corporationdistributor, please quote the NK code number and part name which are listed inthis service manual, and the name or model of the unit in which the required partis located. This will help us to promptly attend to your needs. Always use NihonKohden parts and accessories to assure maximum performance from yourinstrument.



Units and Boards

Rank Index NK Code No. Qty DescriptionD 100 JQ-820V 1 Inlet/outlet unitD 200 JQ-821V 1 Left side piping unitD 300 JQ-822V 1 Center piping unitC 400 JQ-823V 1 4-channel liquid sensor unitC 500 MB-820V 1 Sub bath unitC 600 MC-820V 1 CBC measuring unitD 700 MC-821V 1 Flow cytometry piping unitC 800 MO-820V 1 Laser optical unitC 900 MP-520V 3 Pump unitC 1000 MP-820V 1 Complex pump unitC 1100 MP-821V 1 Sample pump unitC 1200 MP-822V 1 Sheath pump unitC 1300 MS-820V 1 Closed sampler unitC 1400 MS-821V 1 Open sampler unitC 1500 MS-822V 1 Table unitC 1600 PV-820V 1 Display unitD 1700 RK-820V 1 ChassisC 1800 MF-820V 1 Flow cell unitD 1900 ZY-820V 1 Warmer unitC 2000 SC-820VJ 1 Power supply unit (for J type)C 2100 SC-820VK 1 Power supply unit (for K type)C A UT-7146 1 MASTER boardC B UT-7147 1 SLAVE CBC boardC C UT-7148 1 SLAVE MS boardC D UT-7153 1 DRIVER2 boardC E UT-7155 1 DRIVER4 boardC F UT-7160 1 POWER boardC G UT-7161 1 INTERFACE board

RankA Requires replacement within about 1 to 3 months.B Requires replacement within about 6 to 12 months.C Requires replacement when this component has deterioration or damage.D Requires replacement which must be done by service personnel trained in Nihon Kohden or by distributor.



1400

600

1000

1300

400800

1800

900

1500

900

12001100

100

700

300

E

D B

C

A

G

F



Chassis

Rank Index NK Code No. Qty Description

C 7 532639 PharMed tube 1/16 1/8 (7.5 m)(Sampling tube)

C 8 6113-042788A 1 Sampling cupD 9 6111-006457D 1 Top coverD 10 6113-040923C 1 Right coverD 11 6113-040861C 1 Left coverD 12 6111-006484C 1 Front cover

1400 1300

400

900

1500

600

900

87

100

1700




1600

20002100

F

C

A

B

G

D

E

12

11

1700

10

9

1700

700

1100

7

1200

500

200

1900

3001000

1800

800



103

103

231

4

102

104

104

101

101

105

106

103

103

JQ-820V Inlet/Outlet Unit

Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveD 101 2114-072518C 2 Diluent tube joint (tube joint 6)D 102 2114-051042A 2 Nut for diluent tube jointC 103 6114-000591B 4 Detergent/waste tube jointD 104 6114-000074 4 Nut for detergent/waste tube jointD 105 6114-067447B 1 Hemolynac 3 tube joint (tube joint 9)D 106 6114-126134 1 Hemolynac 5 tube joint

* Total quantity per instrument

RankA Requires replacement within about 1 to 3 months.B Requires replacement within about 6 to 12 months.

C Requires replacement when this component has deterioration ordamage.

D Requires replacement which must be done by service personnel trainedin Nihon Kohden or by distributor.



JQ-821V Left Side Piping Unit

Rank Index NK Code No. Qty DescriptionC 4 XP-513V 19* 3-way valve



4

4

4

4

4

4

4



JQ-822V Center Piping Unit

Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveC 301 6113-045321A 1 Liquid chamberC 302 534316 1 O-ring P-30 (Silicone)C 303 2114-065847 1 Rubber packingC 304 2114-080812 1 StopperC 305 6113-043154 1 Liquid chamber capD H UT-7171 1 LIQUID LEVEL SENSOR board



H

305

302

301

303304

1

3

2

4



JQ-823V 4-channel Liquid Sensor Unit

Rank Index NK Code No. Qty DescriptionC 401 6113-034476A 4 Bubble sensorC I UT-7179 1 4WAY LIQUID SENSOR board


I

401



MB-820V Sub Bath Unit

Rank Index NK Code No. Qty DescriptionD 501 553242 2 Stepping motorC 502 YZ-0283 2 Sub bath 2 assy


501

502



MC-820V CBC Measuring Unit


Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveC 5 XP-512V 17* 2-way valveC 601 6113-043305A 1 Air trapC 602 6114-126143 1 Air trap capC 603 315375A 1 O-ring P-8 (Red silicone)C 604 6114-095505D 1 Aperture cap (aperture: 100 m in dia.)C 605 6114-095514D 1 Aperture cap (aperture: 80 m in dia.)C 606 553198 1 O-ring P-10A (Silicone)C 607 553233 1 O-ring P-10A (Red Silicone)C 608 556827 2 O-ring P-10AC 609 YS-020B6 2 BathC 610 6114-036641 1 Hemolysing reagent inlet capA 611 T802 2 Hemoglobin filterB 612 2114-082062B 2 Filter packing SC 613 YZ-0286 2 Counter assyC 614 YZ-0287 2 Vacuum chamber (electrode excluded)C 615 YZ-0288 2 Air chamberC J UT-7143 1 PRE AMP board




614

613

614

615

615

609

610

612

611 6

02 603

601

607

605

608 6

0660

460

8

13

2

1

3

4

5

J

2

611

612

13

2

5

5

a

a

613



MC-821V Flow Cytometry Piping Unit

Rank Index NK Code No. Qty Description C 1 553144 100* O-ring P-3 C 2 6114-095942B 100* Tube fitting nut C 3 6114-095951A 100* Tube stopper C 4 XP-513V 19* 3-way valve C 5 XP-512V 16* 2-way valve C 701 6113-043305A 1 Air trap C 702 6114-126143 1 Air trap cap C 703 315375A 1 O-ring P-8 (Red silicone) C 704 6113-042271 1 Mixing chamber C 705 6114-120327A 1 Mixing chamber cap C 706 393174 1 O-ring P-16 (Silicone) C 707 459264 1 O-ring AS568-004 (Silicone) D 708 723611 3 Pinch valve TPV-N040

B 709-1 709-2

YZ-0282 2 each Pinch valve tube set (709-1: L60, 709-2: L55)

C 710 YZ-0293 1 Silicone tube set (for flow cell unit) D 711 6114-123048A 1 Tube joint ID05 D 712 6114-122994A 1 Tube joint ID1F D 713 6114-123003A 4 Tube joint ID1 D 714 2114-051015B 1 Tube ring D 715 6114-015495B 1 Valve joint A C K UT-7152 1 DRIVER1 board





709-

1

709-

2

705

706

704

707

5

55

713

45

713

708K

708

708

713

713

710

711 2 3 1

702

703

701

709-

1

712



1803

1802

1804

1801

8011805

1806

1806

MF-820V Flow Cell Unit

Rank Index NK Code No. Qty DescriptionC 801 532639 PharMed tube 1/16 1/8 (7.5 m)D 1801 295538 1 O-ring AS568-003D 1802 6114-124118 1 O-ring 1 4 (Silicone)D 1803 530597A 1 Flow cellD 1804 6114-129318 1 Flow cell baseD 1805 565586 2 Steel ball 6D 1806 358524A 2 O-ring P-6 (Silicone)




MO-820V Laser Optical Unit

Rank Index NK Code No. Qty DescriptionC 710 YZ-0293 1 Silicone tube set (for flow cell unit)

C 801 532639 PharMed tube 1/161/8 (7.5m)


1800

710

801



MP-520V Pump Unit

Rank Index NK Code No. Qty DescriptionD 6 551895 21* Photo-interruptorA 901 T462 3 Pump tube assyC 902 YS-003B43 3 Pump rotor assy



901 902

6



MP-820V Complex Pump Unit


Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveC 4-2 XP-503V 3* 3-way valveC 1001 487296 2 Universal couplingC 1002 6113-034485B 1 Cylinder blockD 1003 6114-048495B 1 Cylinder bottom coverD 1004** 6114-108341 1 10 mL pistonD 1005 6144-006261A 1 50 L piston assyD 1006** 6114-083456B 1 10 mL piston guideD 1007** 487313 1 Cap sealC 1008 535903 1 BALSEAL ID20 (X55409B)C 1009 6114-123396 1 50 L piston guideC 1010 315348A 1 O-ring P-5 (Silicone)C 1011 536029 1 BALSEAL ID1.4 (X55408A)C 1012 6113-041085A 3 Cylinder blockD 1013 6114-125839A 3 Cylinder bottom coverD 1014 6114-114272B 3 2 mL pistonC 1015 535868 2 BALSEAL ID8 (X203528A)D 1016 6144-006279B 2 Ball screw assyC 1017 1 BALSEAL ID8 (-)D L UT-7177 1 MD/MP connection board set

* Total quantity per instrument** 1004, 1006 and 1007 are not replaceable locally. When 1004, 1006 or 1007

replacement is necessary, you must replace the assembly which includes 1004,1006 and 1007. The assembly model number will be registered in the nearfuture. Contact Nihon Kohden about the availability.



2

13

1012

1015

1008

1002

101010091011

1001

1001

4

4-24-2

4-2

1006

1004

1003

1007

1014

1013

1005

1016

1016

L

L

1017



1101

1102

1104

132

5

5

6

6

1106 1103

1105

MP-821V Sample Pump Unit

Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 5 XP-512V 17* 2-way valveD 6 551895 21* Photo-interruptorC 1101 487296 1 Universal couplingC 1102 6113-043145A 1 Cylinder blockD 1103 6114-114503B 1 Cylinder bottom coverC 1104 544234 1 BALSEAL ID3 (X200886C)D 1105 6114-114539B 1 250 L pistonD 1106 6114-114548A 1 Lead screw P2





MP-822V Sheath Pump Unit


Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 5 XP-512V 17* 2-way valveC 1201 6113-045321A 1 Liquid chamberC 1202 534316 1 O-ring P-30 (Silicone)C 1203 2114-065847 2 Rubber packingC 1204 2114-080812 2 StopperC 1205 6113-043154 1 Liquid chamber capC 1206 107938 3 O-ring P-6C 1207 534102 PharMed tube 3/32 7/32 (7.5m)C 1208 559147 PharMed tube 3 5 (7.5m)C 1209 541371 1 Check valveC 1210 548195 1 Air filterD 1211 534174 1 CompressorD 1212 548159 10 Hose clipD 1213 534192 1 RegulatorC 1214 6113-043305A Air trapC 1215 6114-126143 Air trap capC 1216 315375A O-ring P-8 (Red silicone)C M UT-7158 1 SHEATH CONTROL boardD H UT-7171 1 LIQUID LEVEL SENSOR board




1203

1204

1201

1202

1205

2 3

1

1207

1208

120

9 120

8

1207

1206

1

1208

1210

5

1203

12

04

a

a

M

5

5

5

5

3 2

1207

1208

1211

1212

1213

H

1214

1216

1215



MS-820V Closed Sampler Unit

Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveD 6 551895 21* Photo-interruptorC 1301 534637 1 Timing belt (Polyurethane rubber)C 1302 534655 1 Timing belt 40 S2M 126C 1303 534673 1 Timing belt 40 S2M 184C 1304 534887 1 Timing belt 40 S2M 256C 1305 534992 1 Timing belt 40 S2M 328C 1306 6114-115075B 1 Rinse unitC 1307 6114-115084B 1 Sampling nozzle guideC 1308 295538 1 O ring AS568-003C 1309 315366 1 O ring AS568-004C 1310 6114-122227C 1 Waste cupB 1311 2114-080153A 1 Waste cup packingB 1312 YZ-0261 1 Sampling nozzle setB 1313 YZ-0262 1 Cap pierce nozzle setA 1314 T802 1 Hemoglobin filter assyD 1315 6114-028285C 1 Roller headD 1316 6114-130397A 1 Monovette roller headD 1317 534441 1 DC motorD 1318 6114-114878B 1 Lead screw (MIX)D 1319 6113-039818B 2 Sampler ball screwC N UT-7154 1 DRIVER3 boardD O UT-7185 1 3POS SENSOR board





1312

13071308130613091313

2 1314

1311

1310

1301

13024

4

4

1303

1305 1304132

N

O

6

1319

6

6

1315

1318

1317



1401

1402

1407

1406

1404

1405

1403

P

1408

6

MS-821V Open Sampler Unit

Rank Index NK Code No. Qty DescriptionD 6 551895 21* Photo-interruptorC 1401 553153 1 Timing belt 40 S2M 176C 1402 553162 1 Timing belt 40 S2M 272C 1403 6113-034467B 1 Manual rinse unitC 1404 6114-102418A 1 Manual rinse unit capC 1405 6114-108475 1 Seat spacerC 1406 315366 1 O ring AS568-004

A 1407YZ-0193(T444B)

1 Manual sampling nozzle

D 1408 6113-026823C 1 Lead screwD P UT-7065 1 SENSOR board







1502

1501

S

1503

1504

Q

R

MS-822V Table Unit

Rank Index NK Code No. Qty DescriptionC 1501 535164 1 Timing belt 40 S2M 460C 1502 535173 1 Timing belt 40 S2M 796D 1503 6111-007171 1 Rack tableD 1504 6124-035659 1 Table caution labelD Q UT-7086 1 SENSOR3 boardD R UT-7157 1 TABLE SENSOR boardC S UT-7156 1 DRIVER5 board






PV-820V Display Unit

Rank Index NK Code No. Qty DescriptionD 1601 601707 1 Color LCDD 1602 601716 1 Touch screenD 1603 539463 1 Inverter boardD 1604 6121-001176C 1 Display front panelD 1605 6111-006475B 1 Display front coverD T UT-7178 1 Front panel board set


1604

1605

16011602

T

T

T

1603



ZY-820V Warmer Piping Unit

Rank Index NK Code No. Qty DescriptionC 1 553144 100* O-ring P-3C 2 6114-095942B 100* Tube fitting nutC 3 6114-095951A 100* Tube stopperC 4 XP-513V 19* 3-way valveD 1901 536983 1 Tubed warmerD U UT-7159 1 WARMER CONTROL board



4

4

1901

U

1

2

3



ZK-821V/VG CCD Bar Code Reader Unit (Option)

Rank Index NK Code No. Qty DescriptionD 2201 6144-010933B 1 Sample tube roller


2201

mek8222 trm b

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