”Arbeitsgruppe NMR - MRI- MR-MicroImaging“

Molecule (Lipid)

nmselforganization

Liposomes

50-500nm

Biosensor3D-MR-Micro-Imaging of a survival plant (submillimeter)

Technology

Application

•Life and Materials Sciences

•Drug Encapsulation

•Online Observation of Destruction of Tumors

•Seeds: Protection, Germination

•Composite Materials

•Drug penetration through the skin (online observation)

........

A

PD Dr. Frank Volke Magnetic Resonance

3He / 1H lung coil for

Siemens Scanner 1,5 T

Technical Solutions (e.g.)

PD Dr. Frank Volke Magnetic Resonance

MRI-Endoskop:

NMR-Spektroskopie und MRI

PD Dr. Frank Volke Magnetic Resonance

• MRI-Endoskop (endoluminal) Cooperation: Univ.-Heidelberg/MR Inside

• Foldable coil, stabilization, positioning via optical channel, works with Siemens 1,5 T clinical Scanner. Very good homogeneity and resolution (compare MRI Image and microscope Image)

2 mm

NMR-MRI-µMRI

Biofilms (flow, structure, forces)

B. Manz, F. Volke, D. Goll, H. Horn, Measuring Local Flow Velocities and Biofilm Structure in Biofilm Systems with Magnetic Resonance Imaging (MRI),Biotechnology and Bioengineering 84, 424-432 (2003).

13C-1H correlation spectrum(MAS at 8000 Hz) of a purePOPC /water dispersion (2H2O)at 310 K

Volke, HRMAS in Biomembrane ResearchBRUKER REPORT 145: ISSN 0724-0185

NOESY 1H

NMR-MRI-µMRI

Xenopus laevis

• Single cell imaging at 600 MHz Proton-NMR (14,1 T)

• The effect of strong magnetic fields on cells

Daniel Mietchen @

PD Dr. Frank Volke Magnetic Resonance

Rimwith QuiecentCells

Necrotic zone

NutrientSolution

Proliferating CellsMC Cell

Multicellular Tumor Spheroid

NMR Micro Imaging (on-line). Effect of Agents on Tumor Spheroids

On-line monitoring of drug effects during several hours

Imaging of structural and motional properties

Diagnostic tool

Dr. Frank Volke; IBMT,

Cells

MCTS

In vivoMicro imaging

Calculated Diffusion Images

0,0 mm 3,0 mm

0,0 mm

3,0 mm

11 h nach HEMAD[10-9 m²s-2 ]

0

40,0 mm 3,0 mm

0,0 mm

3,0 mm

16 h nach HEMAD[10-9 m²s-2 ]

0

4

Jörg Breidt

vor HEMA

PD Dr. Frank Volke Magnetic Resonance

NMR-MRI-µMRI

Quo vadis ?

•Miniaturization!!!

•Combination of MR with Complementary Methods

(US,Laser, IR, OCT)

•Micro-flow Imaging

•Lab-on-chip (Micro-coils)

•Broad range of composite materials, incl. organic ones: e.g.

•Cryobiology, Hydration

•Biofilms, Surface characterization

•Drug-encapsulation

PD Dr. Frank Volke Magnetic Resonance