mikes cv form 1 - ohio university

Michael D. Boyle

1

CURRICULUM VITAE

Name: Michael D. P. Boyle

Education:

1960-1967 Royal Belfast Academical Institution, Belfast, Northern Ireland

1967-1971 B.Sc. (with honors) Biochemistry, University of Glasgow, Glasgow, Scotland

1971-1974 Ph.D., Chester Beatty Research Institute, Belmont, Sutton, Surrey, England

(Thesis: Mechanism(s) of Tumor Cell Destruction by Alloimmune

Macrophages, In Vitro)

Brief Chronology of Employment:

1974-1976 Visiting Fellow, Humoral Immunity Section, Laboratory

of Immunobiology (formerly Immunochemistry Section,

Biology Branch), DCBD, National Cancer Institute, NIH,

Bethesda, Maryland

1976-1980 Expert, Humoral Immunity Section, Laboratory of

Immunobiology, DCBD, National Cancer Institute, NIH,

Bethesda, Maryland

1980 Visiting Scientist, Humoral Immunity Section, Laboratory of

Immunobiology, DCBD, National Cancer Institute, NIH

Bethesda, Maryland

1981-1985 Associate Professor, Department of Immunology and

Medical Microbiology, (Tenure 1983)

Associate Professor, Department of Pediatrics

University of Florida, College of Medicine,

Gainesville, Florida

1985-1988 Professor, Department of Immunology and Medical Microbiology,

Professor, Department of Pediatrics

University of Florida, College of Medicine, Gainesville, Florida

1988-1990 Adjunct Professor, Department of Immunology and

Medical Microbiology, Professor, Department of Pediatrics

University of Florida, College of Medicine


1988-2002 Professor, Department of Microbiology and Immunology

Medical College of Ohio, Toledo, Ohio

11/96-4/97 Visiting Professor, Department of Medicine, University of Florida,


1998-2002 Adjunct Professor of Bioengineering, Department of Bioengineering,

The University of Toledo College of Engineering

Michael D. Boyle

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2002-2012 William J. von Liebig Professor of Biomedical Sciences, Department of

Biology, Juniata College, Huntingdon, Pennsylvania.

Research Interests:

Molecular Pathogenesis

Immunochemistry

Biotechniques

Wound Healing

Tumor Biology

Honors and Other Special Scientific Recognition

1971-1974 Holder of Institute of Cancer Research Studentship

1976-1990 Member, Foundation for the Advancement of Education in the Sciences

1977-present Member, American Association of Immunologists

1977-1992 Member, American Association for Cancer Research

1982-1988 Member, Southeastern Immunology Conference

1984 Stop! Children's Cancer Service Award

1985 Recipient of the University of Florida College of Medicine, Basic Research Prize

1985-1990 Member, American Association of Veterinary Immunology

1986-present Member, American Society of Microbiologists

1987 Senior Faculty Research Award, University of Florida, Chapter of Sigma Xi

1988-present Member Lancefield Society

1989-present Full Member Sigma Xi

1995-1997 American Society for Microbiology Foundation Lecturer

1999 Recipient of the Graduate Dean's Award for mentoring.

1999-2002 Alternate Counselor, American Society for Microbiology, Division E

(Immunology)

2003-2004 Counselor, American Society for Microbiology, Division E

(Immunology)

2003-2004 Ad hoc Nominating Committee to elect Members at Large on the Council

Policy Committee, American Society of Microbiology

2004-2005 President Allegheny Branch, American Society of Microbiology.

2008-2009 Co-director of the newly created Juniata College Center for the Scholarship of

Teaching and Learning

2010. Recipient of the Beachley Award for outstanding teaching.

Appointments to University Committees

1981 Search Committee for faculty member in the School of Veterinary Medicine

Michael D. Boyle

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1983-1985 Member, Senate University of Florida

1984 Search Committee for faculty member in the school of Pharmacy,

University of Florida, College of Pharmacy

1985 Search Committee for faculty member in the School of Veterinary

Medicine University of Florida

1984-1986 Search Committee for Chairman, Department of Immunology and

Medical Microbiology, University of Florida College of Medicine.

1985 Member, Associate Professor Promotion and Tenure Committee

University of Florida College of Medicine

1986-1987 Member Selection Committee for College of Medicine Basic Research

Prize, University of Florida College of Medicine

1987-1988 Member, Associate Professor Promotion and Tenure

Committee, University of Florida, College of Medicine

1988-1989 Member L.C.M.E. Subcommittee on Basic Science and Graduate Education

1993-2002 Department Representative to the Academic Senate, Medical College of Ohio

1995-1996 Member Academic Senate Steering Committee

1995-1999 Member Conflict of Interest Committee; Chairman 1997-1999

1996 Member L.C.M.E. Subcommittee on Clinical Departments

1997-1998 Member Search Committee for Faculty Member in the Department of

Microbiology and Immunology

1997-1998 Member Tenure Committee, Medical College of Ohio

1998 Member Search Committee for Director, Center for Classroom of the Future

1998-1999 Member Evaluation Subcommittee of Medical School Curriculum Committee

1998-1999 Member Search Committee for Assistant Professor of Microbiology and

Immunology

1998-1999 Member Search Committee for Chair, Department of Pathology

1999 Member Selection Committee M.C.O. Research Award

1999-2002 Member Senate Faculty Affairs Committee

2001-2002 Co-Chair, M.C.O. and University of Findlay disaster-preparedness and

bioterrorism working group.

2001-2002 Member M.C.O. Bioterrorism response group.

2002-2009 Member and Chair of the von Liebig Committee.

2006-2012 Member of the Honorary Degree Committee Juniata College.

2009-2012 Convener of the Juniata College grants group.

Editorial and Peer-review Activities

Editor

Michael D. Boyle

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1990 Michael D. P. Boyle, Editor, Bacterial immunoglobulin-binding proteins,

Volumes I and II, Academic Press, San Diego, California.

1993 Michael D. P. Boyle, Editor, Bacterial IgG-binding proteins, ImmunoMethods.

1993 Michael D. P. Boyle, Editor, Analysis of host-pathogen interactions. Implications for

pathogenesis, Special Issue Journal of Microbiological Methods.

2000 Michael D. P. Boyle and Richard Lottenberg, Co-Editors, Analysis of the role of the

plasminogen system in microbial pathogenesis, Methods, in press.

Editorial boards

1983-1987 Associate Editor, Molecular and Cellular Biochemistry

1986-2009 Member, Editorial Board, Journal of Microbiological Methods

1986-2012 Member Editorial Board Biotechniques

1989-1990 Member Research Section Editorial Board, Diagnostics and Clinical Testing

1993-2012 Member, Editorial Board, Methods (formerly ImmunoMethods)

Journal Review

Ad Hoc reviewer

Journal of Immunology Molecular Immunology

Journal of Infectious Disease Science

Journal of Immunological Methods Cancer Research

Infection and Immunity Gene

Journal of the National Cancer Institute Protein Engineering

Canadian Journal of Microbiology Journal of Bacteriology

European Journal of Biochemistry Microbiology

Biochemistry Proteomics

Proceedings of the National Academy of Sciences

Grant review:

Member N.C.I. Program Project Site Visit team, University of Philadelphia, 1989

Member N.I.H. Special Experimental Immunology Study Section, August 1988

Member NIDR Study Section for Research Centers in Oral Biology, 1992-1993

Member N.I.H. SBIR Study Section, September 1992

Member NIH SBIR Study Section, November 2000

Member American Heart Association Southern and Ohio Valley Research Consortium Peer

Review Committee 2001

Ad Hoc Reviewer:

N.I.H. - Bacteriology and Mycology Study Section

N.S.F. -Cellular Physiology Section; Engineering Directorate; S.B.I.R. program

United States Department of Agriculture

Michael D. Boyle

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American Heart Association, National; Florida Affiliate; Ohio Affiliate

American Cancer Society, National; Florida Affiliate; Ohio Affiliate

March of Dimes

Veterans Administration Merit Review Program

Department of Energy CRDA-program

Wellcome Foundation

Israeli Medical Research Council

Pennsylvania Greenhouse

Undergraduate medical and allied health education

Organizational activities

1981-1988 Joint Coordinator, Principles of Immunology for Graduate

Students, University of Florida, College of Medicine

1981-1988 Joint Coordinator, Phase A Microbiology for Medical and Dental

Students, University of Florida, College of Medicine

1983 Dean's Task Force for program in Cell Biology, University of

Florida, College of Medicine

1986 Member Review Group for Curriculum for undergraduate students

enrolled in the College of Liberal Arts and Sciences, University of Florida

1988-2002 Coordinator, Immunology Segment of the Microbiology course for

2nd year Medical Students, Medical College of Ohio

1996 Member Analysis and Synthesis Working Group for Curriculum 2000

Medical College of Ohio

1997-1999 Member Curriculum Review Task Force, Medical College of Ohio

1997-1999 Member Curriculum Committee Years 1 and 2. Medical College of Ohio

1997-1999 Member Curriculum Implementation Group, Medical College of Ohio

1997-1998 Director Immunity and Infection Core for New Curriculum

1999 Co-organizer AMA workshop on Patient Oriented Problem Solving Packages

(POPS) to teach immunology

1999-2002 Co-editor POPS Educational Review Advisory Board

2008-2010 Center for Scholarship and Learning Center Advisory Board.

2012- External Advisor for Juniata College’s Howard Hughes Medical Institute

Undergraduate Award.

Teaching responsibilities

Michael D. Boyle

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Lectures, small group exercises, problem-based learning and laboratory exercises for medical and

dental students in the areas of Immunology, host defense and Microbiology.

Lectures and nurses on topics related to immunology and host defense.

Undergraduate courses in microbiology, with laboratory, and immunology for majors.

Freshman seminar and scientific writing for majors.

Non-major courses on infectious disease and society and the ethical and societal implications of the

human genome project.

Graduate Education

Organizational activities

1981-1988 Full member University of Florida Graduate Faculty

1985-1988 Graduate Coordinator, Department of Immunology and

Medical Microbiology, University of Florida College of Medicine

1986 Judge for Medical guild Graduate Student Research Prize, University

of Florida College of Medicine

1986-1988 Member M.D.-Ph.D. Student Training Program, University of

Florida College of Medicine

1988-2002 Full member Graduate Faculty Medical College of Ohio

1988-2002 Coordinator, Immunology for Graduate Students, Medical College of Ohio

1993-2002 Member Graduate Executive Committee, Medical College of Ohio

1995-2002 Member M.D.-Ph.D. Committee, Medical College of Ohio

1996 Judge, MCO Graduate Student Forum

1996-2002 Member, Bioengineering Center, University of Toledo

1997-2002 Member of Molecular Basis of Disease Ph.D. Program

Teaching responsibilities

Lectures, small group exercises and laboratory rotations for graduate students on topics related to

biotechnology, immunology and host defense. Act as major advisor and serve on graduate student

committees.

Graduate Students (major advisor)

1981-1983 Kathleen J. Reis, Ph.D. dissertation title: "Identification of Bacterial

Fc Receptors and Characterization of a Group C Streptococcal Fc Receptor."

1982-1985 Ervin Faulmann, Ph.D. dissertation title: "Regulation of Serum Protease Activity:

Action of C1-inhibitor on the Activity of Serum C1 and Nerve Growth Factor."

1982-1985 Michele S. Yarnall, Ph.D. dissertation title: "Isolation and Characterization of

Group A Streptococcal Fc Receptors."

Michael D. Boyle

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1983-1985 David Yarnall, MS thesis title: "The Role of C8 and C9 in Determining the

Range of Targets that can be Lysed by Complement."

1985-1989 L. Jeannine Brady, Ph.D. dissertation title: "Isolation and Characterization of

Bacterial IgA Fc Receptors."

1985-1989 Christopher C. Broder, Ph.D. dissertation title: "Characterization of

Bacterial Plasmin Receptors."

1988-1991 Ronald Otten, Ph.D. dissertation title: "Analysis of selective binding activities

of bacterial Fc binding proteins."

1990-1993 Hong Wang, Ph.D. dissertation title: "Plasmin receptors and the invasive

potential of bacteria."

1991-1995 Todd Pack, Ph.D. dissertation title: "Analysis of heterogeneity of bacterial

IgG3-binding proteins."

1992-1997 Roberta Raeder, Ph. D. dissertation title: "The role of surface proteins reactive with

IgG on the pathogenesis of group A streptococcal disease."

1993-1998 Sybil D'Costa, Ph.D. dissertation title: "Analysis of group A streptococcal plasmin-

binding proteins."

1994-1998 Zhu Qing Li, Ph.D. dissertation title: "Molecular mechanism and biological

significance of the interaction between group A streptococci and the human

plasmin(ogen) system."

1999-2002 Myrna Saouda, dissertation title "Analysis of the role of SpeB in group A

streptococcal infections.

1999-2002 Tara Smith, dissertation title "Global regulation of virulence factors in

Streptococcus pyogenes, group A."

2000-2001 Abbey Carr, M.S. thesis title "Analysis of the functional properties of the Oxygen-

stable hemolysin (SLS) of Streptococcus pyogenes."

Undergraduate research.

2002-2012 Mentored 30 undergraduates in research projects at Juniata College. 90% of

these students continued their careers in either graduate school or medical

school.

Michael D. Boyle

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Research-related activities

Academic

1971-1974 Assistant Radiation Control Officer, Department of Tumor Immunology,

Chester Beatty Research Institute, Sutton, Surrey, England

1985-1986 Program Planning Committee for Health Center Academic Wing,

University of Florida, College of Medicine

1989-1992 Institutional Committee on the Care and Use of Laboratory Animals,


1990-1991 Search Committee for Director of Laboratory Animal Medicine,


1990-1992 Member Dean's Committee on Research Space, Medical College of Ohio

1994- present Member Patent Committee, Medical College of Ohio

1994- present Member, Space Utilization Committee, NW Ohio Technology Park

1996-1997 Chairman ad hoc Committee on Intellectual Property Policy, Medical

College of Ohio

1997-1999 Member Medical College of Ohio Research Strategic Planning Committee

1997- 1998 Member New Programs and Technology Subcommittee Medical College,

of Ohio

1999-2002 Medical College of Ohio Liaison to Edison Biotechnology Center

2000-2002 Medical College of Ohio representative on the Board of Regents task force

on Biotechnology

Academic – Industrial

1985 University of Florida Health Center representative to the Governor's Task Force

on Biotechnology

1985-1988 Department of Pediatrics representative to the University of Florida, College of

Medicine Advisory Committee on Biotechnology

1990 Testified to National Biotechnology Panel on funding for Academic

Biotechnology, September 1990

1999-2002 Member of the Board of Directors, Center for Technology

Commercialization, Toledo, Ohio

1999-2002 Member, Technology Advisory Group, Center for Technology

Commercialization, Toledo, Ohio

1999 Member Edison Incubator Director's Council

1999-2000 Member, National Business Incubator Association

1999-2002 Medical College of Ohio Coordinator for SBIR/STTR Grant Writing

Consortium [Director, Pat Snyder, BioStart, Cincinnati, OH]

Michael D. Boyle

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Technology transfer activities

1986-1993 President and Founder of Gator Microbiologicals Inc. a biotechnology

company formed to transfer bacterial IgG-binding protein technology

from my academic laboratory to the market place.

Gator Microbiologicals was taken over byReceptorPro in 1993.

2009-present Member board and chairman of scientific advisory board of PEARL.

Consultant

1989-present Consultant to Blake Laboratories, Great Neck, New York

1993-2000 Consultant to ReceptorPro, Toledo, Ohio

1996 Consultant NW Ohio Venture Fund, Toledo, Ohio

1996-present Consultant - Morphogenesis, Alachua, Florida

2007-present Consultant – APD Life Sciences, State College, PA.

Patents

US Patent # Title Issue date

US patent #4,883,754 Bacterial Fc receptors Issued November 28, 1989

US patent #4,900,660 Streptococcal FcRc Issued February 13, 1990

US patent #4,945,157 Novel extraction procedure for

protein G Issued July 31, 1990

US patent #4,948,725 Type VI Fc receptors Issued August 19, 1990

US patent #4,977,082 Type VI Fc receptors Issued December 11, 1990

US patent #5,071,756 Bacterial Fc Receptors Issued December 10, 1991

US patent #5,085,894 Novel type VI bacterial Fc

receptors Issued February 4, 1992

US patent #5,082,931 Type II bacterial Fc receptors Issued January 21, 1992

US patent #5,098,827 Novel bacterial markers for

pathogenic group B streptococci Issued March 24, 1992

US patent #5,237,050 Bacterial plasmin receptors as

fibrinolytic agents Issued August 17, 1993

US patent #5,328,996 Bacterial plasmin receptors as

fibrinolytic agents Issued July 12, 1994

U.S. patent #5,472,696 Antigen of group B streptococci Issued December 5, 1995

U.S. patent #5,721,339 Gamma antigen of pathogenic

group B streptococci Issued February 24, 1998

U.S. patent #6,136,323

Bacterial plasmin receptors as

fibrinolytic agents Issued October 24, 2000

U.S. patent #61/283,417 General method for generating Pending

Michael D. Boyle

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ultra-high affinity binding

proteins.

Funding History: (*indicates award involving industrial collaboration)

Date Agency Title Amount P.I.

1981-1982 National Institutes of

Health-BSRG

Antibody complement

killing of nucleated cells

$3,000 Michael D. P. Boyle

1982-1983 American Cancer

Society (ACS-82-

067)

Susceptibility of leukemic

cells to antibody and

complement


1982-1983 Milheim Foundation

(09551-00)

Purging of tumor cells from

bone marrow


1983-1985 American Heart

Association, Florida

Affiliate

Clq Receptors and

Pathogenesis of Bacterial

Endocarditis



Health

Role of Fc Reactive

Proteins in the Pathogenesis

of Poststreptococcal

Nephritis



Health

Elimination of Neuroblasts

from Bone Marrow with

Antibody and Complement


*1986-1987 Cilco Activation of Complement

Pathways by Intraoccular

Lens



Health

Role of Fc Reactive

Proteins in the Pathogenesis

of Poststreptococcal

Nephritis


1985-1988 National Science

Foundation

Use of Bacterial Fc Reactive

Proteins in

Immunotechnology



Association Florida

Affiliate

Role of Plasmin Receptors

in Bacterial Endocarditis

Program Project Grant with

Drs. Lottenberg, Gee, and

Shands

$228,391 Richard Lottenberg

Principal Investigator

Michael D. P. Boyle

Co-Prin. Investigator


Health

Islet Cell Autoantibodies

and the Natural History of

IDD

$312,389 Noel McClaren

Principal

Investigator

Michael D. Boyle,

Co-prin. Investigator

*1989-1990 Blake Laboratories Isolation and

characterization of a group

B streptococcal IgA-binding


Michael D. Boyle

11

protein

Michael D. Boyle

12


*1989-1990 Blake Laboratories Cloning a Group B

streptococcal IgA-binding

protein

$37,500 Ervin Faulmann,

Principal

Investigator

Michael D. Boyle,



Health

Characterization of a

streptococcal plasmin

receptor

$427,254

Subcon.

$100,227

Richard Lottenberg,

Principal

Investigator

Michael D. Boyle,



Association Ohio

Affiliate

Use of bacterial plasmin

receptor - plasmin

complexes as plasminogen

activators



Health-BSRG

Role of type II IgG-

binding proteins in group

A streptococcal infections


*1993 AMVAX, Inc. Isolation of a non-IgA-

binding form of the group

B

streptococcal beta antigen


*1993-1995 Ohio Edison

Biotechnology Center,

Biomedical

Partnership Grant

Production of

streptococcal protein G


*1994-1995 Blake Laboratories Preparation of

recombinant protein B


*1994-1995 National Institutes of

Health - STTR

Bacterial binding proteins

for human IgM



Health


streptococcal plasmin

receptor

$628,300 Richard Lottenberg,

Principal

Investigator Michael

D. P. Boyle, Co-Prin.

Investigator


Association (Ohio

Affiliate)

Influence of cytokines on

group A streptococcal

infections

$90,000 Dennis W. Metzger,

Principal

Investigator Michael

D. P. Boyle, Co-Prin.

Investigator

1993-1999

National Institutes of

Health

Human IgG subclass-

specific bacterial binding

proteins


Michael D. Boyle

13



Health STTR program

Phase II

Bacterial binding proteins

for human IgM


In collaboration with

ReceptorPro.

1998-2000 American Medical

Association

Patient-Oriented Problem

Solving (POPS) system

for teaching immunology

$48,000 P. Small, M.D.

Principal

Investigator

Michael D. P. Boyle

Co-Investigator

1999-2000 Ohio Department of

Development

Life Sciences Incubator

Feasibility Study



Health

Interaction of group A

streptococci with human

plasminogen system



Health (RO1)

Immunoproteomics $450,000 Michael D. P. Boyle,

*2002-

2004

National Institutes of

Health STTR Program

Identification of a bacterial

IgE-binding protein

$180,000

(sub-

contract)

In collaborator with

Biocheck Labs,

Toledo Ohio

*2002-

2004

Life Sciences

Greenhouse of

Central Pennsylvania

Identification of a bacterial

IgY-binding protein

$71,000


Biocheck Labs,

Toledo Ohio


Health (R03)

Immunoproteomics and

detection of viral disease

$100,000

In collaboration with

Dr. Mark Buller, St

Louis University

2004 Center for

Educational

Technology

Implications of the NIH

Roadmap for

Undergraduate Life

Sciences Education

$14,734 Michael D. P. Boyle,

*2005 Life Sciences

Greenhouse of

Central Pennsylvania

Business Opportunity

Validation Grants

$25,000


Biocheck Labs,

Toledo Ohio

*2005 National Institutes of

Health/SBIR

Low-cost Affinity system

for Preparation of

plasma/serum for

Biomarker Analysis

$41,576 In collaborator with

APDLS, State

College, PA.


Association)


Putative Strepcoagulase

$120,000

Michael D. P. Boyle


Health (R21)

Characterization of

streptococcal IDES


*2010 PEARL Biosciences

State College PA.

Genetically engineer high

affinity IgG binding protein


Michael D. Boyle

14

*2011-

2012

PEARL Biosciences

State College PA.

IgA binding protein $23,765 Michael D. P. Boyle

2011-2012 National Science

Foundation

RCN UBE Transforming

Undergraduate Education

through Increased Faculty

Access to NextGen

Sequencing Runs


BIBLIOGRAPHY

Michael D. P. Boyle

Peer-reviewed articles

1. Boyle, M.D.P. and Ormerod, M.G. (1975) The destruction of tumor cells by alloimmune

peritoneal cells: Mechanism of action of activated macrophages in vitro. J.

Reticuloendothel. Soc. 17:73-83.

2. Boyle, M.D.P. and Ormerod, M.G. (1975) The destruction of allogeneic tumor cells by

peritoneal macrophages from immune mice: Purification of lytic effectors. Cell. Immunol.

13:247-258.

3. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1975) Lysis of tumor cells by antibody and

complement. III. Lack of correlation between antigen movement and cell lysis. J.

Immunol. 115:473-475.

4. Boyle, M.D.P. and Ormerod, M.G. (1976) Destruction of allogenic tumor cells by

peritoneal macrophages: Production of lytic effectors by immune mice. Transplantation

21:242-246.


complement. VI. Enhanced killing of enzyme pretreated tumor cells. J. Immunol.

116:661-668.

6. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Detection of complement-dependent

antibody to tumor cells in sera of strain-2 guinea pigs cured of their tumors by BCG

treatment. J. Natl. Can. Inst. 56:623-626.

7. Jolley, G.M., Boyle, M.D.P. and Ormerod, M.G. (1976) The destruction of allogenic

tumor cells by antibody and adherent cells from peritoneal cavities of mice. Cell.

Immunol. 22:262-270.

Michael D. Boyle

15

8. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1976) Studies on the terminal stages of

antibody-complement mediated killing of a tumor cell. I. Evidence for the existence of an

intermediate T*. J. Immunol. 116:1272-1275.


antibody-complement mediated killing of a tumor cell. II. Inhibition of transformation of

T* to dead cells by 3'5' cAMP. J. Immunol. 116:1276-1279.


antibody-complement mediated killing of a tumor cell. III. Effect of membrane active

agents. J. Immunol. 117:106-109.


complement. VII. Complement-dependent 86Rb release--A non-lethal event? J. Immunol.

117:1346-1350.

12. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) Effect of Concanavalin A on the

function activity of hemolytic antibody. Immunol. Commun. 6:283-296.

13. Boyle, M.D.P. and Ohanian, S.H. (1977) Quantitation of antibody-complement mediated

lysis of tumor cells. J. Immunol. Methods. 15:135-146.

14. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) Effect of Concanavalin A on the

classical complement pathway. J. Immunol. 118:1622-1625.

15. Boyle, M.D.P., Langone, J.J., Ohanian, S.H. and Borsos, T. (1977) Effect of

Concanavalin A on the killing of tumor cells by antibody and complement. J. Immunol.

118:1626-1630.

16.* Schlager, S.I., Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1977) Effect of inhibiting

DNA, RNA, and protein synthesis of tumor cells on their susceptibility to killing by

antibody and complement. Cancer Res. 37:1432-1437.

17. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1977) 125 I Protein A: Applications to the

quantitative determination of fluid phase and cell-bound IgG. J. Immunol. Methods.

28:281-293.

18. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Complement-dependent cytotoxic

antitumor antibody. I. Immunoglobulin class determined by interaction with Protein A or

Concanavalin A. J. Natl. Can. Inst. 60:411-418.

19. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1978) Effect of protease treatment on the

sensitivity of tumor cells to antibody-GPC killing. Clin. Immunol. Immunopathol. 10:84-

94.

Michael D. Boyle

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20. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1978) Studies on the terminal stages of

immune hemolysis. III. Distinction between the insertion of C9 and the formation of a

transmembrane channel. J. Immunol. 120:1721-1725.

21. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Studies on the interaction between

Protein A and immunoglobulin G. I. Effect of Protein A on the functional activity of IgG.

J. Immunol. 121:327-332.

22. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1978) Studies on the interaction between

Protein A and immunoglobulin G. II. Composition and activity of complexes formed

between Protein A and IgG. J. Immunol. 121:333-338.

23. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1978) Effect of lectins on the hemolytic

activity of complement components. Immunochemistry 15:465-470.

24. Langone, J.J., Boyle, M.D.P. and Borsos, T. (1979) A solid phase immunoassay for

human immunoglobulin E. Use of 125I Protein A as the tracer. Analyt. Biochem. 93:207-

215.

25. Boyle, M.D.P., Ohanian, S.H. and Borsos, T. (1979) Antigenic distinction between human

C9 and guinea pig C9. Clin. Immunol. Immunopathol. 13:103-113.

26. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1979) Studies on the terminal stages of

immune hemolysis. IV. Effect of metal salts. J. Immunol. 122:1209-1213.

27. Lo, T.N. and Boyle, M.D.P. (1979) Relationship between the intracellular cyclic

adenosine 3':5'-monophosphate level of tumor cells and their sensitivity to killing by

antibody and complement. Cancer Res. 39:3158-3162.

28. Boyle, M.D.P., Langone, J.J. and Borsos, T. (1979) 1,10 Phenanthroline mediated

hemolysis--Evidence that 1,10 phenanthroline does not substitute specifically for C9.

Molec. Immunol. 16:307-312.

29. Boyle, M.D.P. and Borsos, T. (1979) Studies on the terminal stages of immune hemolysis.

V. Evidence that not all complement-produced transmembrane channels are equal. J.

Immunol. 122:71-76.

30. Boyle, M.D.P. and Langone, J.J. (1979) Complement-dependent cytotoxic antitumor

antibody. II. Quantitative determination of cell bound IgM. J. Natl. Can. Inst. 62:1537-

1544.

31. Boyle, M.D.P., Gee, A.P. and Borsos T. (1979) Studies on the terminal stages of immune

hemolysis. VI. Osmotic blockers of differing Stokes' radii detect complement-induced

transmembrane channels of differing size. J. Immunol. 122:77-82.

Michael D. Boyle

17

32. Gee, A.P., Borsos, T. and Boyle, M.D.P. (1979) Interaction between components of the

human classical complement pathway and immobilized Cibacron Blue F3GA. J. Immunol.

Methods. 30:119-126.

33. Boyle, M.D.P. and Langone, J.J. (1980) A simple procedure to use whole serum as a

source of either IgG or IgM specific antibody. J. Immunol. Methods. 32:51-58.

34. Boyle, M.D.P. and Borsos, T. (1980) The terminal stages of immune hemolysis-A brief

review. Molec. Immunol. 17:425-432.

35. Gee, A.P., Boyle, M.D.P. and Langone, J.J. (1980) Effect of Concanavalin A on the

hemolytic activity of the components of the classical complement pathway. Molec.

Immunol. 17:143-146.

36. Boyle, M.D.P. and Ohanian, S.H. (1980) Evidence for the influence of the initial

complement components on the assembly and activity of the membrane attack complex. J.

Immunol. 124:2824-2827.

37. Gee, A.P., Boyle, M.D.P. and Borsos, T. (1980) Distinction between C8-mediated and

C8/C9-mediated hemolysis on the basis of independent 86Rb and hemoglobin release. J.

Immunol. 124:1905-1910.

38. Okada, M., Boyle, M.D.P. and Borsos, T. (1980) Evidence for a temperature-dependent

and temperature-independent pathway in the generation of complement-mediated

transmembrane channels. Biochem. Biophys. Res. Commun. 94:406-412.

39. DeLisi, C., Boyle, M.D.P. and Borsos, T. (1980) Analysis of the colloid osmotic step of

complement-mediated immune hemolysis. J. Immunol. 125:2055-2062.

40. DeLisi, C., Boyle, M.D.P. and Borsos, T. (1980) Mathematical analysis of the reaction of

EAC1-8 with C9: Identification of parameters defining conditions for molecular titration.

J. Immunol. 125:2334-2338.

41. Boyle, M.D.P., Gee, A.P., Okada, M. and Borsos, T. (1980) Differences in cell-bound C8

sites on chicken erythrocytes measured by their reactivity with guinea pig and human C9.

J. Immunol. 125:2818-2822.

42. Boyle, M.D.P. (1981) Inhibition by zinc of the binding of C1 to antigen-antibody

complexes. Biochem. Biophys. Res. Commun. 103:856-862.

43. Boyle, M.D.P., Gee, A.P. and Borsos, T. (1981) Heterogeneity in the size and stability of

transmembrane channels produced by whole complement. Clin. Immunol. Immunopathol.

20:287-295.

Michael D. Boyle

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44. Gee, A.P., Boyle, M.D.P. and Borsos, T. (1981) Differences in the functional activity of

human and guinea pig C1 based on their reactivity with IgG at low temperatures.

Molecular Immunology 18:609-613.

45. Boyle, M.D.P. and Young, M. (1982) Nerve growth factor: Activation of the classical

complement pathway by specific substitution for component C1. Proc. Natl. Acad. Sci.

USA 79:2519-2522.

46. Reis, K.J., Ayoub, E.M. and Boyle, M.D.P. (1983) Detection of receptors for the Fc

region of IgG on Streptococci. J. Immunol. Methods 59:83-94.

47. Gee, A.P., Boyle, M.D.P., Lawman, M.J. P., Munger, K.L. and Young M. (1983) Nerve

growth factor: Stimulation of polymorphonuclear leukocyte chemotaxis in vitro. Proc.

Natl. Acad. Sci. 80:7215-7218.

48. Barrett, D.J. and Boyle, M.D.P. (1984) Restoration of complement function in vitro by

plasma infusion in factor I (C3b inactivator) deficiency. J. Pediatr. 104:76-81.

49. Reis, K.J., Boyle, M.D.P., and Ayoub, E.M. (1984) Identification of distinct Fc-receptor

molecules on streptococci and staphylococci. J. Clin. Lab. immunol. 13:75-80.

50. Boyle, M.D.P. (1984) Is the membrane attack complex of complement an enzyme? Mol.

Cell. Biochem. 61:5-1553.

51. Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1984) Streptococcal Fc receptors: I.

Isolation and partial characterization of the receptor from a group C streptococcus. J.

Immunol. 132:3091-3097.

52. Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1984) Streptococcal Fc receptors: II.

Comparison of the reactivity of a receptor from a group C streptococcus with

staphylococcal protein A. J. Immunol. 132:3098-3102.

53. Lawman, M.J.P., Thurmond, M.C., Reis, K.J., Gauntlett, D.R., and Boyle, M.D.P. (1984)

Solid phase radioimmunoassay for the detection of immunoglobulins against bovine

Brucella abortus. Vet. Immunol. Immunopathol. 6:291-305.

54. Lawman, M.J.P., Boyle, M.D.P., Gee, A.P., and Young, M. (1984) A rapid technique for

measuring leukocyte chemotaxis in vitro. J. Immunol. Methods 69:197-206.

55. Reis, K.J., Yarnall, M., Ayoub, E.M. and Boyle, M.D.P. (1984) Effect of mouse passage

on Fc receptor expression by group A streptococci. Scand. J. Immunol. 20:433-439.

56. Boyle, M.D.P. (1984) Applications of bacterial Fc receptors in immunotechnology.

Biotechniques 2:334-340.

Michael D. Boyle

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57. Gee, A.P., van Hilten, J., Graham-Pole, J., and Boyle, M.D.P. (1985) Antibody

complement killing of neuroblasts. Progress in Clinical and Biological Research 175:471-

483.

58. Boyle, M.D.P., Lawman, M.J.P., Gee, A.P., and Young, M. (1985) Nerve growth factor:

A chemotactic factor for polymorphonuclear leukocytes in vitro. J. Immunol. 134:564-568.

59. Yarnall, M., Reis, K.J., Ayoub, E.M., and Boyle, M.D.P. (1985) An immunoblotting

technique for the detection of bound and secreted bacterial Fc receptors. J. Microbiol.

Methods. 3:83-93.

60. Gee, A.P., Rolfe, A.E., Worthington-White, D., Graham-Pole, J., and Boyle, M.D.P.

(1985) A rapid alternative to the clonogenic assay for measuring antibody and complement

mediated killing of tumor cells. Clin. Immunol. Immunopathol. 34:263-271.

61. Young, M., Gee, A.P., Munger, K.L., Lawman, M.J.P., and Boyle, M.D.P. (1985)

Leukocyte chemotaxis mediated by nerve growth factor. A non-proteolytic function of the

proteolytic active site. Mol. Cell. Biochem. 66:65-69.

62. Gee, A.P., Bruce, K.M., Morris, T.M. and Boyle, M.D.P. (1985) Evidence for an anti-

complementary factor associated with human bone marrow cells. J. Natl. Cancer Inst.

75:441-445.

63. Reis, K.J., Ayoub, E.M. and Boyle, M.D.P. (1985) A rapid method for the isolation and

characterization of a homogeneous population of streptococcal Fc receptors. J. Microbiol.

Methods 4:45-58.

64. Boyle, M.D.P., Wallner, W.A., von Mering, G.O., Reis, K.J., and Lawman, M.J.P. (1985)

Interaction of bacterial Fc receptors with goat immunoglobulin. Mol. Immunol. 22:1115-1121.

65. Lawman, M.J.P., Boyle, M.D.P., Gee, A.P. and Young, M. (1985) Nerve growth factor

accelerates the early cellular events associated with wound healing. Exp. Mol. Pathol.

43:274-281.

66. Lawman, M.D.P., Joiner, S., Gauntlett, D.R., and Boyle, M.D.P. (1985) A rapid

purification for bovine IgG2. Evidence for IgG2a and IgG2b sub-classes. Compar.

Immunol. Microbiol. and Infect. Dis. 8:1-8.

67. Yarnall, M., and Boyle, M.D.P. (1986) Identification of a unique receptor on a group A

streptococcus for the Fc region of human IgG3. J. Immunol. 136:2670-2673.

68. Yarnall, M., and Boyle, M.D.P. (1986) Influence of Dipeptides on Interaction of

Immunoglobulins with Bacterial Fc Receptors. Biochem. Biophys. Res. Comm. 135:1105-

1111.

Michael D. Boyle

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69. Yarnall, M. and Boyle, M.D.P. (1986) Isolation and partial characterization of a type II Fc

receptor from a group A streptococcus. Mol. and Cell Biochem. 70:57-66.

70. Lawman, M.J.P., Ball, D.R., Hoffman, E.M., Des Jardin, L.E., and Boyle, M.D.P. (1986)

Production of Brucella abortus specific protein A reactive antibodies (IgG2) in infected and

vaccinated cattle. Vet. Microbiol. 12:43-53.

71. Yarnall, M., Ayoub, E.M., and Boyle, M.D.P. (1986) Analysis of Surface Receptor

Expression on Bacteria Isolated from Patients with Endocarditis. J. Gen. Microbiology

132:2049-2051.

72. von Mering, G.O. and Boyle, M.D.P. (1986) Comparison of type III Fc receptors

associated with group C or group G streptococci. Mol. Immunol. 23:811-821.

73. Yarnall, D.P. and Boyle, M.D.P. (1986) Restriction in the Lytic Efficiency of Complement

on Different Erythrocyte Targets: A Re-examination of the Activities of Horse C8 and C9.

Mol. Immunol., 23:823-829.

74. Yarnall, M., and Boyle, M.D.P. (1986) Isolation and Characterization of Type IIa and

Type IIb Streptococcal Fc Receptors. Scand J. Immunol. 24:549-557.

75. Gee, A.P., Bruce, K.M., van Hilten, J., Siden, E.J., Braylan, R.C., Bauer, P.C., and Boyle,

M.D.P. (1987) Selective Loss of Expression of a cALLa Associated antigen on a Human

Leukemic Cell Line Induced by Treatment with Monoclonal Antibody and Complement. J.

Natl. Cancer Inst. 78:29-35.

76. Boyle, M.D.P. and Gee, A.P. (1987) Low antigen density tumor cells - an obstacle to

effective autologous bone marrow purging? Cancer Invest. 5:113-118.

77. Faulmann, E., Young, M., and Boyle, M.D.P. (1987) Interaction of nerve growth factor

and C1 inhibitor. J. Immunol. 138:4336-4340.

78. Faulmann, E., and Boyle, M.D.P. (1987) Regulation of activation of C1 in serum.

Molecular Immunol. 24:655-660.

79. Boyle, M.D.P., Chiodo, V.A., Lawman, M.J.P., Gee, A.P., and Young, M. (1987)

Urokinase: A chemotactic factor for polymorphonuclear leukocytes in vitro. J. Immunol.

139:169-174.

80. Boyle, M.D.P. and Reis, K.J. (1987) Bacterial Fc receptors. Biotechnology 5:697-703.

81. Lottenberg, R., Broder, C., and Boyle, M.D.P. (1987) Identification of a specific receptor

for plasmin on a group A streptococcus. Infect. Immun. 55:1914-1928.

Michael D. Boyle

21

82. Wallner, W.A., Lawman, M.J.P., and Boyle, M.D.P. (1987) Reactivity of bacterial Fc

receptors with bovine immunoglobulins: Implications and limitations for quantitative

immunochemistry. Appl. Microbiol. Biotechnol. 27:168-173.

83. Reis, K.J., Siden, E.J., and Boyle, M.D.P. (1988) Selective Colony Blotting to Expand

Bacterial Surface Receptors: Applications to Receptors for Rat Immunoglobulins.

Biotechniques 6:130-136.

84. Gee, A.P. and Boyle M.D.P. (1988) Purging Tumor Cells From Bone Marrow by Use of

Antibody and Complement: A Critical Appraisal. J. Natl. Cancer Inst. 80:154-159.

85. Reis, K.J., von Mering, G.O., Karis, M.A., Faulmann, E.L., Lottenberg, R., and Boyle,

M.D.P. (1988) Enzyme-labeled type III bacterial Fc receptors - a versatile tracer for

immunoassay. J. Immunol. Methods 107:273-280.

86. Feher, S.L., Boyle, M.D.P., and Halliwell, R.E.W. (1988) Identification of protein A from

Staphylococcus intermedius isolated from canine skin. Am. J. Vet. Res. 49:697-701.

87. Broeseker, T.A., Boyle, M.D.P., and Lottenberg, R. (1988) Characterization of the

interaction of human plasmin with its specific receptor on a group A streptococcus.

Microbial Pathogenesis 5:19-27.

88. Brady, L.J., Daphtary, U.D., Ayoub, E.M., and Boyle, M.D.P. (1988) Two novel antigens

associated with group B streptococci identified by a rapid two stage radioimmunoassay. J.

Infect. Dis. 158:965-972.

89. Young, M., Blanchard, M.H., Sessions, F., and Boyle, M.D.P. (1988) The subunit structure

of high molecular weight mouse nerve growth factor. Biochemistry 27:6675-6681.

90. DesJardin, L.E., Lottenberg, R., and Boyle, M.D.P. (1989) Group A streptococci bind

human plasmin but not other structurally related proteins. Thrombosis Res. 55:187-193.

91. Brady, L.J., and Boyle, M.D.P. (1989) Identification of non-immunoglobulin A-Fc-

binding forms and low-molecular-weight secreted forms of the group B streptococ

antigen. Infect. Immun. 57:1573-1581.

92. Broder, C.C., Lottenberg, R., and Boyle, M.D.P. (1989) Mapping of the domain of human

plasmin recognized by its unique group A streptococcal receptor. Infect. Immun. 57:2597-

2605.

93. Faulmann, E.L., Otten, R.A., Barrett, D.J., and Boyle, M.D.P. (1989) Immunological

Applications of Type III Fc Binding Proteins: Comparison of Different Sources of Protein

G. J. Immunological Methods 123:269-281.

Michael D. Boyle

22

94. Coleman, S.E., Brady, L.J., and Boyle, M.D.P. (1990) Colloidal gold immunolabelling of

Immun., 58:332-340.

95. Heath, D.G., Boyle, M.D.P. and Cleary, P.P. (1990) Isolated DNA repeat region from

fcrA76, the Fc-binding protein gene from an M-type 76 strain of group A streptococci,

encodes a protein with Fc-binding activity. Mol Microbiol., 4:2071-2079.

96. Raeder, R., Otten, R.A., and Boyle, M.D.P. (1991) Isolation and partial characterization of

a type IV bacterial immunoglobulin binding protein. Mol Immunol., 28:661-671.

97. Raeder, R., Faulmann, E.L., and Boyle, M.D.P. (1991) Evidence for functional

heterogeneity in IgG Fc binding proteins associated with group A streptococci. J.

Immunol., 146:1247-1253.

98. Chun, C.Y., Brady, L. J., Boyle, M.D.P., Dillon, H. C., and Ayoub, E. M. (1991) Group B

streptococcal C protein-associated antigens: Association with neonatal sepsis. J. Infect.

Dis.,163:786-791.

99. Raeder, R., Otten, R.A., and Boyle, M.D.P. (1991) Comparison of albumin receptors

expressed on bovine and human group G streptococci Infect. Immun., 59:609-616.

100. Broder, C.C., Lottenberg, R., von Mering, G.O., Johnston, K.H., and Boyle, M.D.P. (1991)

Isolation of a prokaryotic plasmin receptor: Relationship to a plasminogen activator

produced by the same micro-organism. J. Biol. Chem. 266:4922-4928.

101. Faulmann, E.L., and Boyle, M.D.P. (1991) A simple preparative procedure to extract and

purify protein G from group G streptococci. Preparative Biochem. 21:75-86.

102. Faulmann, E. L., Duvall, J. L., and Boyle, M. D. P. (1991) Protein B: A versatile bacterial

Fc-binding protein selective for human IgA, Biotechniques 10:748-755.

103. Raeder, R. A. and Boyle, M. D. P., (1991) Detection of rheumatoid-like factors in serum of

chickens immunized with bacterial immunoglobulin binding proteins. J. Immunol. Methods

138:201-209.

104. Otten, R. A. and Boyle, M. D. P. (1991) The mitogenic activity of type III bacterial

immunoglobulin binding proteins (protein G) for human peripheral blood lymphocytes is

not related to their ability to react with human serum albumin or IgG , J. Immunol.

146:2588-2595.

105. Otten, R. A. and Boyle, M. D. (1991) Characterization of protein G expressed by human

group C and G streptococci, J. Microbiol. Methods 13:185-200.

106. Otten, R. A., Raeder, R. A., Heath, D. G., Lottenberg, R., Cleary, P. P., and Boyle,

Michael D. Boyle

23

M. D. P. (1992) Identification of two type IIa IgG-binding proteins expressed by a single

group A streptococcus. J. Immunol. 148:3174-3182..

107. Lottenberg, R., DesJardin, L. E., Wang, H. and Boyle, M.D.P. (1992) Streptokinase

producing streptococci grown in human plasma acquire unregulated cell-associated plasmin

activity. J. Infect. Dis. 166:436-440.

108. Lottenberg, R., Broder, C.C., Boyle, M.D.P., Kain, S. J., Schroeder, B.L. and Curtiss, R.

(1992) Cloning, sequence analysis, and expression in Escherichia coli of a streptococcal

plasmin receptor. J. Bact. 174:5204-5210.

109. Raeder, R., Otten, R., Chamberlin, L., and Boyle, M.D.P. (1992) Functional and

serological analysis of type II IgG-binding proteins expressed by pathogenic group A

streptococci. J. Clin. Microbiol., 30:3074-3081.

110. Raeder, R. and Boyle, M.D.P. (1993) Association between expression of immunoglobulin

G-binding proteins by group A streptococci and virulence in a mouse skin infection model.

Infect. Immun., 61:1378-1384.

111. Boyle, M.D.P. and Raeder, R. (1993) Analysis of heterogeneity of IgG-binding proteins

expressed by group A streptococci. ImmunoMethods, 2:41-55.

112. Raeder, R. and Boyle, M.D.P. (1993) Association of type II IgG-binding protein

expression and survival of group A streptococci in human blood. Infect. Immun., 61:3696-

3702.

113. Boyle, M.D.P., Faulmann, E.L., and Metzger, D.W. (1993) Use of Antibodies for Analysis

of Bacterial Proteins, J. Microbiol. Methods, 18:289-303.

114. Boyle, M.D.P. (1993) Analysis of host-bacterial interactions - implications for

pathogenesis, J. Microbiol. Methods, 18:163-165.

115. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1994) Analysis of plasmin (ogen)

acquisition by clinical isolates for group A streptococci incubated in human plasma. J.

Infect. Dis. 169:143-149.

116. Lottenberg, R., Minning-Wenz, D. and Boyle, M.D.P. (1994) Capturing host plasmin

(ogen): a common mechanism for invasive pathogens? Trends in Microbiol. 2:20-24.

117. Boyle, M.D.P., Hawlitzky, J. Raeder, R. and Podbielski, A. (1994) Analysis of the genes

encoding two unique type IIa IgG-binding proteins expressed by a single group A

streptococcus. Infect. Immun, 62:1336-1347.

Michael D. Boyle

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118. Pack, T.D., Otten, R.A., Raeder, R.H. and Boyle, M.D.P. (1994) Identification of two

functional forms of IgG3-binding protein expressed by group A streptococci. Infect.

Immun, 62:2104-2107.

119. Podbielski, A., Hawlitzky, J., Pack, T.D., Flosdorff, A. and Boyle, M.D.P. (1994) A group

A streptococcal Enn protein potentially resulting from intergenomic recombination exhibits

atypical immunoglobulin-binding characteristics. Mol. Microbiol., 12:725-736.

120. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1995) A role for fibrinogen in the

streptokinase-dependent acquisition of plasmin(ogen) by group A streptococci J. Infect.

Dis. 171:85-92.

121. Boyle, M.D.P. (1995) Variation of multifunctional surface binding proteins - a virulence

strategy for group A streptococci? J. Theoretical Biol., 173:415-426.

122. Boyle, M.D.P., Weber-Heynemann, J., Raeder, R. and Podbielski, A. (1995) Characterization of

a gene coding for a type IIo bacterial IgG-binding protein. Mol. Immunol., 32:669-678.

123. Wang, H., Lottenberg, R. and Boyle, M.D.P. (1995) Analysis of the Interaction of Group

A Streptococci with Fibrinogen, Streptokinase and Plasminogen. Microbial Pathogen.,

78:153-166.

124. Raeder, R. and Boyle, M.D.P. (1995) Analysis of IgG-binding expression by invasive

isolates of Streptococcus pyogenes. Clin. Diagnos. Lab. Immunol., 2:484-486.

125. Raeder, R. and Boyle, M.D.P. (1995) Distinct profiles of IgG-binding protein expression

by invasive M1 serotype isolates of Streptococcus pyogenes. Clin. Diagnos. Lab.

Immunol., 2:478-483.

126. Metzger, D.W., Raeder, R., Van Cleave, V.H. and Boyle, M.D.P. (1995) Protection of

mice from group A streptococcal skin infection by interleukin 12. J. Infect. Dis., 171:1643-

1645.

127. Pack, T.D. and Boyle, M.D.P. (1995) Characterization of a type II'o group A streptococcal

immunoglobulin-binding protein. Mol. Immunol., 32:1235-1243.

128. Podbielski, A., Schnitzler, N., Beyhs, P. and Boyle, M.D.P. (1996) M-related protein

(Mrp) contributes to group A streptococcal resistance to phagocytosis by human

granulocytes. Mol. Microbiol., 19:429-441.

129. Christner, R. and Boyle, M.D.P. (1996) Role of staphylokinase in the acquisition of

plasmin(ogen)-dependent enzymatic activity by staphylococci. J. Infect. Dis., 173:104-

112.

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130. Pack, T.D., Podbielski, A., and Boyle, M.D.P. (1996) Identification of an amino acid signature sequence predictive of protein G-inhibitable IgG3-binding activity in group A streptococcal IgG-binding proteins. Gene, 171:65-70.

131. Raeder, R. and Boyle, M.D.P. (1996) Properties of IgG-binding proteins expressed by

Streptococcus pyogenes isolates are predictive of invasive potential. J. Infect. Dis.,

173:888-895.

132. Krebs, B., Kaufhold, A., Boyle, M.D.P. and Podbielski, A. (1996) Different alleles of the

fcrA/mrp gene of Streptococcus pyogenes encode M-related proteins exhibiting an identical

immunoglobulin-binding pattern. Med. Microbiol. Immunol., 185:39-47.

133. Tsivitse, M. and Boyle, M.D.P. (1996) Identification of distinct IgG-subclass binding

domains within a group A streptococcal type IIo IgG-binding protein. Can. J. Microbiol.,

42:1172-1175.

134. Christner, R., Li, Z., Raeder, R., Podbielski, A. and Boyle, M.D.P. (1997) Identification of

key gene products required for acquisition of plasmin-like enzymatic activity by group A

streptococci. J. Infect. Dis., 175:1115-1120.

135. Boyle, M.D.P. and Lottenberg, R. (1997) Plasminogen Activation by Invasive Human

Pathogens. Thrombosis Haemostasis, 77:1-10.

136. Talanin, N.Y., Shelley, W.B., Raeder, R., Shelley, E.D. and Boyle, M.D.P. (1997)

Detection of streptococcal class I M protein in psoriasis by confocal immunofluorescent

microscopy. Acta Dermator-Venereologica, 77:175-180.

137. D’Costa, S., Wang, H., Metzger, D. and Boyle, M.D.P. (1997) Group A streptococcal

isolate 64/14 expresses surface plasmin-binding structures in addition to Plr. Res. in

Microbiol., 148:559-572.

138. Schmidt, K-H., Podbielski, A., Raeder, R. and Boyle, M.D.P. (1997) Inactivation of single

genes within the virulence regulon of an M2 group A streptococcal isolate in differences in

virulence for chicken embryos and for mice. Mol. Pathogen., 23:347-355.

139. Li, Z., Gutierrez, J. A., Bleiweis, A.S., and Boyle, M.D.P. (1997) Efficient Insertional

Mutagenesis In Group A Streptococci Mediated By Tn917 Transposon. J. Micro. Methods.,

30:205-215.

140. Boyle, M.D.P., Raeder, R., Flosdorff, A., and Podbielski, A. (1998) Role of emm and mrp

genes in the virulence of group A streptococcal isolate 64/14 in a mouse model of skin

infection. J. Infect. Dis., 177:991-997.

141. Boyle, M.D.P., Vogel, L., Sisson, S., and Raeder. R. (1998) Do chickens immunized with

bacterial IgG-binding proteins produce rheumatoid factor-like antibodies? Scand. J. of

Immunol., 47:218-222.

Michael D. Boyle

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142. Donabedian, H., and Boyle, M.D.P. (1998) Clot formation by group A streptococci.

Infect. Immun., 60:2362-2364.

143. D'Costa, S., and Boyle, M.D.P. (1998) Interaction of a group A streptococcus within

human plasma results in assembly of a surface plasminogen activator that contributes to

occupancy of surface plasmin-binding structures. Microb. Path., 24:341-349.

144. Raeder, R., Woischnik, M., Podbielski, A., and Boyle, M.D.P. (1998) A secreted

streptococcal cysteine protease can cleave a surface expressed M1 protein and alter the

immunoglobulin-binding properties. Res. Microbiol., 149:539-548.

145. Li, Z., Ploplis, V., French, E.L., and Boyle, M.D.P. (1999) Interaction between group A

streptococci and the plasmin(ogen) system promotes virulence in a mouse skin infection

model. J. Infect. Dis., 179:907-914.

146. Li, Z., Sledjeski, D., Kreikemeyer, B., Podbielski, A. and Boyle, M.D.P. (1999)

Identification of pel, a S. pyogenes locus that affects both surface and secreted proteins. J.

Bacteriol., 181:6019-6027.

147. Schroeder, B., Boyle, M.D.P., Sheerin, B.R., Asbury, A.C. and Lottenberg, R. (1999)

Species-specificity of plasminogen activation and the acquisition of surface-associated

proteolytic activity by group C streptococci grown in plasma. Infect. Immun. 67:6487-

6495.

148. Raeder, R., Barker-Merrill, L., Lester, T., Boyle, M.D.P. and Metzger, D.W. (2000) A

pivotal role for interferon-gamma in protection against group A streptococcal skin

infection. J. Infect. Dis., 181:639-645.

149. Raeder, R., Horokopathis, E., Hollingshead, S., and Boyle, M.D.P. (2000) Absence of

SpeB Production in Virulent Large Capsular Forms of Group A Streptococcal Strain 64.

Infect. Immun., 68:744-751.

150. Yamakami, K., Yoshizawa, N., Wakabayashi, K., Takeuchi, A., Tadakuma, T. and Boyle,

M.D.P. (2000) The potential role for nephritis associated plasmin receptor in acute post-

streptococcal glomerulonephritis. Methods, 21:185-197.

151. D'Costa, S., and Boyle, M.D.P. (2000) Interaction of group A streptococci expressing Glu-

plasminogen-binding M proteins with the human plasminogen system. Methods, 21:165-

177.

152. Kreikemeyer, B., Boyle, M.D.P., Buttaro, B.A., Heinemann, M. and Podbielski, A. (2000)

Group A streptococcal growth phase-associated virulence factor regulation by a novel

operon (Fas) with homologies to two-component type regulators requires a small RNA

molecule. Mol. Micro., 39:392-406.

Michael D. Boyle

27

153. D'Costa, S., Romer, T.G. and Boyle, M.D.P. (2000) Analysis of expression of a cytosolic

enzyme on the surface of Streptococcus pyogenes. Biochem. Biophys. Res. Commun.,

278:826-832.

154. Boyle, M.D.P., Romer, T.G., Meeker, A.K. and Sledjeski, D. (2001) Use of surface

enhanced laser desorption ionization protein chip system to analyze streptococcal exotoxin

B activity secreted by Streptococcus pyogenes. J. Microb. Meth., 46:87-97.

155. Sledjeski, D.D., Coco, C., Corriher, C. and Boyle, M.D.P. (2001) Isolation of human

plasma-inducible, growth phase- and temperature-regulated gene fusions in Streptococcus

pyogenes using a Tn917-lacZ transposon. J. Micro. Meth., 46:107-117.

156. Bai, H., Buller, R.M.L., Chen, N. and Boyle, M.D.P. (2001) Viral binding proteins as

surrogates in immunoassays for immune mediators. BioTechniques, 32.160-171.

157. Saouda, M., Wu, W., Conran, P. and Boyle, M.D.P. (2001) Streptococcal pyrogenic

exotoxin B (SpeB) enhances tissue damage initiated by other Streptococcus pyogenes

products. J. Infect. Dis., 184:723-731.

158. Carr, A., Sledjeski, D.D., Podbielski, A., Boyle, M.D.P. and Kreikemeyer, B. (2001)

Similarities between complement-mediated and streptolysin S-mediated hemolysis. J. Biol.

Chem., 276:41790-41796.

159. Eberhard, T. H., Sledjeski, D.D. and Boyle, M.D.P. (2001) Mouse skin passage of a

Streptococcus pyogenes Tn917 mutant of sagA/pel restores virulence, beta-hemolysis and

sagA/pel expression without altering the position or sequence of the transposon. BMC

Microbiol. 1:33..

160 Saouda, M., Romer T. and Boyle, M.D.P. (2002) Application of immuno-mass

spectrometry to analysis of a bacterial virulence factor. BioTechniques, 32:916-92.

161. Romer T., Rescellah M. and Boyle, M.D.P. (2002) Use of surface enhanced laser

desorption ionization time-of-flight mass spectrometry to analyze post-translational

modification of bacterial virulence factors. Research Signpost. 37:1-18

162. Romer T. and Boyle, M.D.P. (2003) Application of immunoproteomics to analysis of

post-translational processing of the anti-phagocytic M protein of Streptococcus pyogenes.

Proteomics. 3:29-35.

163. Smith T., Sledjeski, D.D. and Boyle, M.D.P. (2003) Regulation of protein H

expression in M1 serotype isolates of Streptococcus pyogenes. FEMS Microbiology

Letters 219:9-15.

Michael D. Boyle

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164. Smith T., Sledjeski, D.D. and Boyle, M.D.P. (2003) Streptococcus pyogenes infection in

mouse skin leads to a time-dependent up-regulation of protein H expression. Infect.

Immun. 71:6079-6082.

165. Rezcallah MS, Boyle MD, Sledjeski DD.(2004) Mouse skin passage of Streptococcus

pyogenes results in increased streptokinase expression and activity. Microbiology

150:365-371.

166. White A.A. and Boyle M.D.P. (2004) 15:1785-1793. Raw data analysis: are students

aware of all the variables? Microbiology Education Review in press.

167. Yoshizawa, N., Wakabayaski, K., Takeuchi, A., Yamakami, K., Tadakuma, T. and Boyle,

M.D.P. (2004) Nephritis-associated Plasmin Receptor and Acute Poststreptococcal

Glomerulonephritis: Characterization of the Antigen and Associated Immune Response.

J. Am. Soc. Nephrology. 15:1785-1793.

168. Hess J., L Blazer, T. Romer, L Faber, R. M. Buller and Boyle, M.D. P(2005)

Immunoproteomics, J. Chromatog. B. 815: 65–75.

169. Blazer L, Denning E, Hemmis C, Boyle M.D. and Mulfinger L. (2005) Monitoring Peptide

Modification by SELDI-TOF Mass Spectrometry , American Laboratory, 37:15-18.

170. Coyle E, Blazer L., White A.A., Hess J.L. and Boyle M.D. (2005) Practical applications of

high-affinity, albumin-binding proteins from a group G streptococcal isolate. Applied

Microbiology and Biotechnology. 27:1-7.

171. Hess JL, Boyle MD. 2006 Fibrinogen fragment D is necessary and sufficient to anchor a

surface plasminogen-activating complex in Streptococcus pyogenes. Proteomics. 6:375-8.

172. Hess JL, Porsch EA, Shertz CA Boyle MD. (2007) Immunoglobulin-cleavage by the

streptococcal cysteine protease IdeS can be detected using protein G capture and mass

spectrometry. J Microbiological Methods , 70:284-91.

173. Boyle MD, Lottenberg R Bacterial adaptation and infection. Inflamm Allergy Drug

Targets. 2010 9(4):238-48.

174. Porsch EA, Shertz CA, Boyle MD. Novel Sample Preparation for Mass Spectral Analysis

of Complex Biological Samples. Curr Proteomics 2010 Jul;7(2):90-101.

175. Buonaccorsi VP, Boyle MD, Grove D, Praul C, Sakk E, Stuart A, Tobin T, Hosler J,

Carney SL, Engle MJ, Overton BE, Newman JD, Pizzorno M, Powell JR, Trun N.

GCAT-SEEKquence: genome consortium for active teaching of undergraduates through

Michael D. Boyle

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increased faculty access to next-generation sequencing data.CBE Life Sci Educ. 2011

10:342-5.

Chapters in Books and Other Non-Peer Reviewed Articles

1. Boyle, M.D.P. and Boros, T. (1983) Tissue damage caused by the direct and indirect

action of complement. In Reticuloendothelial Society Treatise on Immunopathology (eds.

H. Friedman, S. Reichard and M. Escobar). Plenum Press, Chapter 3, pp. 43-76.

2. Boyle, M.D.P., Faulmann, E.L., and Andres, J.M. (1988) Complement dependent

inflammatory reactions mediated by Type III bacterial Fc receptors. In Inflammatory

Bowel Disease: Current Status and Future Approaches (ed. R.P. MacDermott). Excerpta

Medica. 775:371-376.

3. Boyle, M.D.P., Lawman, M.J.P., Gee, A.P., and Young, M. (1988) Measurement

leukocyte chemotaxis In Vivo. (G. DiSabato, ed.) Methods in Enzymology, 162:101-114.

4. Boyle, M.D.P. (1990) The type I bacterial immunoglobulin binding protein:

Staphylococcal Protein A. In Bacterial Immunoglobulin Binding Proteins Volume I

(M.D.P. Boyle, ed.) Academic Press, Chapter 2 pp. 17-28.

5. Boyle, M.D.P. (1990) Complement Activation and Bacterial Immunoglobulin Binding

Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle, ed.)

Academic Press, Chapter 25 pp. 295-304.

6. Boyle, M.D.P. and Reis, K.J. (1990) Antigenic Relationships Among Bacterial IgG

Binding Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,

ed.) Academic Press, Chapter 15 pp. 175-186.

7. Brady, J.L. and Boyle, M.D.P. (1990) IgA Fc Binding Proteins Associated with Group B

Streptococci. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,


8. Faulmann, E.L. and Boyle, M.D.P. (1990) Type IIa and Type IIb Immunoglobulin

Binding Proteins Associated With Group A Streptococci. In Bacterial Immunoglobulin

Binding Proteins Volume I (M.D.P. Boyle, ed.) Academic Press, Chapter 6 pp. 69-83.

9. Reis, K.J. and Boyle, M.D.P. (1990) Type III Fc Immunoglobulin Binding Proteins:

Isolation and Characterization from a Group C Streptococcus. In Bacterial

Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle, ed.) Academic Press, Chapter

8 pp. 101-112.

Michael D. Boyle

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10. Reis, K.J., Salpeter, J. and Boyle, M.D.P. (1990) Type IV Bacterial Immunoglobulin

Binding Proteins. In Bacterial Immunoglobulin Binding Proteins Volume I (M.D.P. Boyle,


11. Reis, K.J. and Boyle, M.D.P. (1990) Isolation and Characterization of a Type VI Bacterial

Immunoglobulin Binding Protein. In Bacterial Immunoglobulin Binding Proteins Volume

I (M.D.P. Boyle, ed.) Academic Press. Chapter 14 pp. 165-174.

12. Boyle, M.D.P., Faulmann, E.L., Otten, R. and Heath, D. (1990) Streptococcal

Immunoglobulin Binding Proteins. E. M. Ayoub, G. H. Cassell, W. C. Branche, T. J. Henry

(eds.) Microbial Determinants of Virulence and Host Response. A.S. M. Press Washington

D.C. pp. 19-44. Feb. 1989.

13. Boyle, M.D., Lawman, M.J.P., and Gee, A.P. (1990) Application of Fc binding proteins

for the detection of specific antibodies. In Bacterial Immunoglobulin Binding Proteins

Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 181-195.

14. Boyle, M.D.P. (1990) Bacterial immunoglobulin binding proteins and complement. In

Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic

Press, pp. 369-391.

15. Boyle, M.D.P. (1990) Introduction to bacterial immunoglobulin binding proteins. In

Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic

Press, pp. 1-21.

16. Boyle, M.D.P., and Faulmann, E.L. (1990) Application of bacteria expressing

immunoglobulin binding proteins to immunoprecipitation reactions. In Bacterial

Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp.

273-289.

17. Boyle, M.D.P., Reis, K.J., and Faulmann, E.L. (1990) Isolation and functional

characterization of bacterial immunoglobulin binding proteins. In Bacterial

Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 71-

89.

18. Boyle, M.D.P., Reis, K.J., Otten, R.A., and Faulmann, E.L. (1990) Extraction and

monitoring of soluble immunoglobulin binding proteins. In Immunoglobulin Binding

Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 49-70.

19. Brady, L., J. Musselman, C. Chun, E.M. Ayoub, and Boyle, M.D.P. (1990) Use of Fc-

binding proteins to identify cell surface and secreted antigens associated with group B

streptococci, pp. 161-179.

Michael D. Boyle

31

20. Lawman, M.J.P., Gee, A.P., Lawman, P.D., and Boyle, M.D.P. (1990) Measurement of in

vivo leucocyte chemotaxis mediated by Fc-binding proteins. In Bacterial Immunoglobulin

Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 405-415..

21. Otten, R.A., and Boyle, M.D.P. (1990) Bacterial immunoglobulin binding proteins -

future trends. In Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle,

ed.) Academic Press, pp. 425-452.

22. Reis, K.J., and Boyle, M.D.P. (1990) Application of enzyme labeled IgG binding proteins

in immunoassay. In Bacterial Immunoglobulin Binding Proteins Volume II (M.D.P. Boyle,

ed.) Academic Press, pp. 145-160.

23. Reis, K.J., and Boyle, M.D.P. (1990) Detection and enhancement of expression of

bacterial cell surface immunoglobulin binding proteins. In Bacterial Immunoglobulin

Binding Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 23-47.

24. Reis, K.J., and Boyle, M.D.P. (1990) Production of polyclonal antibodies to

immunoglobulin binding proteins. In Bacterial Immunoglobulin Binding Proteins Volume

II (M.D.P. Boyle, ed.) Academic Press, pp. 105-124.

25. Reis, K.J., Lawman, M.J.P., and Boyle, M.D.P. (1990) Use of bacteria expressing

immunoglobulin binding proteins in coagglutination assays. In Immunoglobulin Binding

Proteins Volume II (M.D.P. Boyle, ed.) Academic Press, pp. 291-299.

26. Boyle, M.D.P., Faulmann, E. L., and Metzger, D. W. (1993) Applications of bacterial

immunoglobulin-binding proteins to the purification of immunoglobulins. In Molecular

Interactions and Bioseparation (T.T. Ngo, ed.) Plenum Press, pp. 91-112.

27. Boyle, M.D.P. (1993) Bacterial IgG-binding proteins. (M.D.P. Boyle, ed.)

ImmunoMethods, 2:1-3.

28. Boyle, M.D.P. and Metzger, D.W. (1994) Antibody-binding bacterial proteins and

immunoreagents. In Antibody Techniques. A Guide for Nonimmunologists (E.P. Lillehoj

and V.S. Malik, eds.) Academic Press, pp. 177-209.

29. Pack, T.D. and Boyle, M.D.P. (1995) The enn gene of group A streptococcal isolate A992

encodes an IgG3-binding protein. Dev. Biol. Stand. 85: 203-207.

30. Boyle, M.D.P. (1998) Bacterial IgG-binding proteins. Encyclopedia of Immunology. 2nd

edition, pp. 323-327.

31. Boyle, M.D.P. (2000) Immunoglobulin-binding proteins expressed by gram-positive

bacteria. In: Effects of Microbes on the Immune System (Madeleine W. Cunningham,

ed.), Lippincott, Williams & Wilkins, pp.195-217

Michael D. Boyle

32

32. Boyle, M.D.P. and Lottenberg, R. (2000) Approaches to analysis of the role of the

plasmin(ogen) system. Editorial in Special Issue of Methods, 21:99-102.

33. Boyle, M.D.P., Raeder, R. and Podbielski, A. (2001) Ig-Binding Proteins: a Review.

EOS, 20:37-42.

34. Kreikemeyer, B., Boyle, M.D.P., Elsner, A. and Podbielski. A. (2000) Characterization of

a potential signaling peptide affecting group A streptococcal virulence factor expression.

In: Streptococcal and Streptococcal Diseases: Entering the new Millennium. (Diana R.

Martin, John R. Tagg, eds.), Proceedings of the XIV Lancefield International Symposium

on Streptococci and Streptococcal Diseases, pp. 375-379.

35. Yoshizawa, N., Yamakami, K., Fujino M., Oda T., Tamuri, K., Matsumoto K., Sugisaki T.,

and Boyle, M.D.P. (2000) The potential role for NAPLR in acute post-streptococcal

glomerulonephritis. In: Streptococcal and Streptococcal Diseases: Entering the new

Millennium. (Diana R. Martin, John R. Tagg, eds.), Proceedings of the XIV Lancefield

International Symposium on Streptococci and Streptococcal Diseases, pp. 667-671.

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